The aim of this study was to evaluate the visible loss of sugar cane and the damage to the knuckles, using the John Deere 3520 harvester on three different travel speeds (3.0;4.0 and 5.0 km·h-1), in DIC with five...The aim of this study was to evaluate the visible loss of sugar cane and the damage to the knuckles, using the John Deere 3520 harvester on three different travel speeds (3.0;4.0 and 5.0 km·h-1), in DIC with five repetitions in Campos dos Goytacazes, Rio de Janeiro/Brazil. Each treatment was composed by six lines of harvested cane stumps, with a length of 290 m each. In these six lines, the remains of sugar cane left in the field were collected by placing the sampling frame in two central lines every 50 m, and separating 40 m of edge. The sampling area was surrounded by 2 m wide and 10 m long, totaling 20 m2. To calculate the damage to stumps, a visual methodology by [1], was used, which classifies the damage grade, ranging from 1 to 4. Fifty stumps were assessed randomly for each speed, in a sampling area of 1800 m2. The data were submitted to ANOVA and Tukey test at 5%, in order to compare the effect of different speeds on the losses and damages. There was no significant difference regarding the loss or damage by comparing the different speeds. Therefore, it is more advantageous to use the speed of 5.0 km·h-1, harvesting more in less time and causing the same level of damage.展开更多
Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their...Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their seasonal biomass production can be managed to complement forage grasses. Our research objectives were to evaluate both warm- and cool-season annual forage legumes as green manure for biomass, N content, ability to enhance soil organic carbon (SOC) and soil N, and impact on post season forage grass crops. Nine warm-season forage legumes (WSL) were spring planted and incorporated as green manure in the fall. Forage rye (Secale cereale L.) was planted following the incorporation of WSL treatments. Eight cool-season forage legumes (CSL) were fall planted in previously fallow plots and incorporated as green manure in late spring. Sorghum-sudangrass (Sorghum bicolor x Sorghum bicolor var. sudanense) was planted over all treatments in early summer after forage rye harvest and incorporation of CSL treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were evaluated for dry matter and N concentration. Soil cores were taken from each plot, split into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil C and N were measured using combustion analysis. Nylon mesh bags containing plant samples were buried at 15 cm and used to evaluate decomposition rate of above ground legume biomass, including change in C and N concentrations. Mungbean (Vigna radiata L. [Wilczek]) had the highest shoot biomass yield (6.24 t DM ha<sup>-1</sup>) and contributed the most total N (167 kg∙ha<sup>-1</sup>) and total C (3043 kg∙ha<sup>-1</sup>) of the WSL tested. Decomposition rate of WSL biomass was rapid in the first 10 weeks and very slow afterward. Winter pea (Pisum sativum L. spp. sativum), arrow leaf clover (Trifolium vesiculosum Savi.), and crimson clover (Trifolium incarnatum L.) were the most productive CSL in this trial. Austrian winter pea produced 8.41 t DM ha<sup>-1</sup> with a total N yield of 319 kg N ha<sup>-1</sup> and total C production of 3835 kg C ha<sup>-1</sup>. The WSL treatments had only small effects on rye forage yield and N concentration, possibly due to mineralization of N from a large SOC pool already in place. The CSL treatments also had only minimal effects on sorghum-sudangrass forage production. Winter pea, arrow leaf and crimson clover were productive cool season legumes and could be useful as green manure crops. Mungbean and cowpea (Vigna unguiculata [L.] Walp.) were highly productive warm season legumes but may include more production risk in green manure systems due to soil moisture competition.展开更多
Sign language includes the motion of the arms and hands to communicate with people with hearing disabilities.Several models have been available in the literature for sign language detection and classification for enha...Sign language includes the motion of the arms and hands to communicate with people with hearing disabilities.Several models have been available in the literature for sign language detection and classification for enhanced outcomes.But the latest advancements in computer vision enable us to perform signs/gesture recognition using deep neural networks.This paper introduces an Arabic Sign Language Gesture Classification using Deer Hunting Optimization with Machine Learning(ASLGC-DHOML)model.The presented ASLGC-DHOML technique mainly concentrates on recognising and classifying sign language gestures.The presented ASLGC-DHOML model primarily pre-processes the input gesture images and generates feature vectors using the densely connected network(DenseNet169)model.For gesture recognition and classification,a multilayer perceptron(MLP)classifier is exploited to recognize and classify the existence of sign language gestures.Lastly,the DHO algorithm is utilized for parameter optimization of the MLP model.The experimental results of the ASLGC-DHOML model are tested and the outcomes are inspected under distinct aspects.The comparison analysis highlighted that the ASLGC-DHOML method has resulted in enhanced gesture classification results than other techniques with maximum accuracy of 92.88%.展开更多
Currently,individuals use online social media,namely Facebook or Twitter,for sharing their thoughts and emotions.Detection of emotions on social networking sites’finds useful in several applications in social welfare...Currently,individuals use online social media,namely Facebook or Twitter,for sharing their thoughts and emotions.Detection of emotions on social networking sites’finds useful in several applications in social welfare,commerce,public health,and so on.Emotion is expressed in several means,like facial and speech expressions,gestures,and written text.Emotion recognition in a text document is a content-based classification problem that includes notions from deep learning(DL)and natural language processing(NLP)domains.This article proposes a Deer HuntingOptimizationwithDeep Belief Network Enabled Emotion Classification(DHODBN-EC)on English Twitter Data in this study.The presented DHODBN-EC model aims to examine the existence of distinct emotion classes in tweets.At the introductory level,the DHODBN-EC technique pre-processes the tweets at different levels.Besides,the word2vec feature extraction process is applied to generate the word embedding process.For emotion classification,the DHODBN-EC model utilizes the DBN model,which helps to determine distinct emotion class labels.Lastly,the DHO algorithm is leveraged for optimal hyperparameter adjustment of the DBN technique.An extensive range of experimental analyses can be executed to demonstrate the enhanced performance of the DHODBN-EC approach.A comprehensive comparison study exhibited the improvements of the DHODBN-EC model over other approaches with increased accuracy of 96.67%.展开更多
Hyperspectral(HS)image classification is a hot research area due to challenging issues such as existence of high dimensionality,restricted training data,etc.Precise recognition of features from the HS images is importa...Hyperspectral(HS)image classification is a hot research area due to challenging issues such as existence of high dimensionality,restricted training data,etc.Precise recognition of features from the HS images is important for effective classification outcomes.Additionally,the recent advancements of deep learning(DL)models make it possible in several application areas.In addition,the performance of the DL models is mainly based on the hyperparameter setting which can be resolved by the design of metaheuristics.In this view,this article develops an automated red deer algorithm with deep learning enabled hyperspec-tral image(HSI)classification(RDADL-HIC)technique.The proposed RDADL-HIC technique aims to effectively determine the HSI images.In addition,the RDADL-HIC technique comprises a NASNetLarge model with Adagrad optimi-zer.Moreover,RDA with gated recurrent unit(GRU)approach is used for the identification and classification of HSIs.The design of Adagrad optimizer with RDA helps to optimally tune the hyperparameters of the NASNetLarge and GRU models respectively.The experimental results stated the supremacy of the RDADL-HIC model and the results are inspected interms of different measures.The comparison study of the RDADL-HIC model demonstrated the enhanced per-formance over its recent state of art approaches.展开更多
Sentiment Analysis(SA)is one of the subfields in Natural Language Processing(NLP)which focuses on identification and extraction of opinions that exist in the text provided across reviews,social media,blogs,news,and so...Sentiment Analysis(SA)is one of the subfields in Natural Language Processing(NLP)which focuses on identification and extraction of opinions that exist in the text provided across reviews,social media,blogs,news,and so on.SA has the ability to handle the drastically-increasing unstructured text by transform-ing them into structured data with the help of NLP and open source tools.The current research work designs a novel Modified Red Deer Algorithm(MRDA)Extreme Learning Machine Sparse Autoencoder(ELMSAE)model for SA and classification.The proposed MRDA-ELMSAE technique initially performs pre-processing to transform the data into a compatible format.Moreover,TF-IDF vec-torizer is employed in the extraction of features while ELMSAE model is applied in the classification of sentiments.Furthermore,optimal parameter tuning is done for ELMSAE model using MRDA technique.A wide range of simulation analyses was carried out and results from comparative analysis establish the enhanced effi-ciency of MRDA-ELMSAE technique against other recent techniques.展开更多
Minerals are critical in maintaining health and physiological function in wildlife. Geographic variation in soil and forage mineral concentration may predispose wildlife to mineral imbalances, where a common symptom i...Minerals are critical in maintaining health and physiological function in wildlife. Geographic variation in soil and forage mineral concentration may predispose wildlife to mineral imbalances, where a common symptom is restricted somatic growth. We investigated if mineral imbalances could explain localized differences in morphology of white-tailed deer (Odocoileus virginianus) occurring in geographically proximate sites with similar management, climate, and habitat. We collected serum samples and morphological measurements from free-ranging white-tailed deer captured during 2011-2019 from coastal and inland rangeland sites in South Texas, USA. We measured mineral concentrations in serum from captured deer at each location. Asymptotic deer body mass and antler size averaged 8% - 20% smaller for deer at the coastal compared to the inland site. The proportion of deer with deficient levels of serum copper was greater at the coastal site (66% versus 14%). Our results suggest regional mineral deficiencies in deer may limit antler and body development. Wildlife managers should be aware of all aspects of wildlife nutrition and the importance of considering nutrients beyond energy and protein.展开更多
Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b ge...Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies.展开更多
A study was conducted on the identifications of the degraded samples of sika deer (Cervus nippon) and red deer (Cervus elaphus) by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s...A study was conducted on the identifications of the degraded samples of sika deer (Cervus nippon) and red deer (Cervus elaphus) by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical (0.026±0.006), which was smaller than the smallest nucleotide distance between eastern red deer and sika deer (0.036). Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.展开更多
[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 p...[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.展开更多
Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine ...Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.展开更多
基金FAPERJ,for the financial support and for the execution of the research study.
文摘The aim of this study was to evaluate the visible loss of sugar cane and the damage to the knuckles, using the John Deere 3520 harvester on three different travel speeds (3.0;4.0 and 5.0 km·h-1), in DIC with five repetitions in Campos dos Goytacazes, Rio de Janeiro/Brazil. Each treatment was composed by six lines of harvested cane stumps, with a length of 290 m each. In these six lines, the remains of sugar cane left in the field were collected by placing the sampling frame in two central lines every 50 m, and separating 40 m of edge. The sampling area was surrounded by 2 m wide and 10 m long, totaling 20 m2. To calculate the damage to stumps, a visual methodology by [1], was used, which classifies the damage grade, ranging from 1 to 4. Fifty stumps were assessed randomly for each speed, in a sampling area of 1800 m2. The data were submitted to ANOVA and Tukey test at 5%, in order to compare the effect of different speeds on the losses and damages. There was no significant difference regarding the loss or damage by comparing the different speeds. Therefore, it is more advantageous to use the speed of 5.0 km·h-1, harvesting more in less time and causing the same level of damage.
文摘Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their seasonal biomass production can be managed to complement forage grasses. Our research objectives were to evaluate both warm- and cool-season annual forage legumes as green manure for biomass, N content, ability to enhance soil organic carbon (SOC) and soil N, and impact on post season forage grass crops. Nine warm-season forage legumes (WSL) were spring planted and incorporated as green manure in the fall. Forage rye (Secale cereale L.) was planted following the incorporation of WSL treatments. Eight cool-season forage legumes (CSL) were fall planted in previously fallow plots and incorporated as green manure in late spring. Sorghum-sudangrass (Sorghum bicolor x Sorghum bicolor var. sudanense) was planted over all treatments in early summer after forage rye harvest and incorporation of CSL treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were evaluated for dry matter and N concentration. Soil cores were taken from each plot, split into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil C and N were measured using combustion analysis. Nylon mesh bags containing plant samples were buried at 15 cm and used to evaluate decomposition rate of above ground legume biomass, including change in C and N concentrations. Mungbean (Vigna radiata L. [Wilczek]) had the highest shoot biomass yield (6.24 t DM ha<sup>-1</sup>) and contributed the most total N (167 kg∙ha<sup>-1</sup>) and total C (3043 kg∙ha<sup>-1</sup>) of the WSL tested. Decomposition rate of WSL biomass was rapid in the first 10 weeks and very slow afterward. Winter pea (Pisum sativum L. spp. sativum), arrow leaf clover (Trifolium vesiculosum Savi.), and crimson clover (Trifolium incarnatum L.) were the most productive CSL in this trial. Austrian winter pea produced 8.41 t DM ha<sup>-1</sup> with a total N yield of 319 kg N ha<sup>-1</sup> and total C production of 3835 kg C ha<sup>-1</sup>. The WSL treatments had only small effects on rye forage yield and N concentration, possibly due to mineralization of N from a large SOC pool already in place. The CSL treatments also had only minimal effects on sorghum-sudangrass forage production. Winter pea, arrow leaf and crimson clover were productive cool season legumes and could be useful as green manure crops. Mungbean and cowpea (Vigna unguiculata [L.] Walp.) were highly productive warm season legumes but may include more production risk in green manure systems due to soil moisture competition.
基金Princess Nourah bint Abdulrahman University Researchers Supporting Project Number(PNURSP2023R263)Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia+1 种基金The authors would like to thank the Deanship of Scientific Research at Umm Al-Qura Universitysupporting this work by Grant Code:22UQU4310373DSR54.
文摘Sign language includes the motion of the arms and hands to communicate with people with hearing disabilities.Several models have been available in the literature for sign language detection and classification for enhanced outcomes.But the latest advancements in computer vision enable us to perform signs/gesture recognition using deep neural networks.This paper introduces an Arabic Sign Language Gesture Classification using Deer Hunting Optimization with Machine Learning(ASLGC-DHOML)model.The presented ASLGC-DHOML technique mainly concentrates on recognising and classifying sign language gestures.The presented ASLGC-DHOML model primarily pre-processes the input gesture images and generates feature vectors using the densely connected network(DenseNet169)model.For gesture recognition and classification,a multilayer perceptron(MLP)classifier is exploited to recognize and classify the existence of sign language gestures.Lastly,the DHO algorithm is utilized for parameter optimization of the MLP model.The experimental results of the ASLGC-DHOML model are tested and the outcomes are inspected under distinct aspects.The comparison analysis highlighted that the ASLGC-DHOML method has resulted in enhanced gesture classification results than other techniques with maximum accuracy of 92.88%.
基金Princess Nourah bint Abdulrahman University Researchers Supporting Project Number (PNURSP2022R281)Princess Nourah bint Abdulrahman University,Riyadh,Saudi ArabiaDeanship of Scientific Research at Umm Al-Qura University for supporting this work by Grant Code: (22UQU4340237DSR61).
文摘Currently,individuals use online social media,namely Facebook or Twitter,for sharing their thoughts and emotions.Detection of emotions on social networking sites’finds useful in several applications in social welfare,commerce,public health,and so on.Emotion is expressed in several means,like facial and speech expressions,gestures,and written text.Emotion recognition in a text document is a content-based classification problem that includes notions from deep learning(DL)and natural language processing(NLP)domains.This article proposes a Deer HuntingOptimizationwithDeep Belief Network Enabled Emotion Classification(DHODBN-EC)on English Twitter Data in this study.The presented DHODBN-EC model aims to examine the existence of distinct emotion classes in tweets.At the introductory level,the DHODBN-EC technique pre-processes the tweets at different levels.Besides,the word2vec feature extraction process is applied to generate the word embedding process.For emotion classification,the DHODBN-EC model utilizes the DBN model,which helps to determine distinct emotion class labels.Lastly,the DHO algorithm is leveraged for optimal hyperparameter adjustment of the DBN technique.An extensive range of experimental analyses can be executed to demonstrate the enhanced performance of the DHODBN-EC approach.A comprehensive comparison study exhibited the improvements of the DHODBN-EC model over other approaches with increased accuracy of 96.67%.
文摘Hyperspectral(HS)image classification is a hot research area due to challenging issues such as existence of high dimensionality,restricted training data,etc.Precise recognition of features from the HS images is important for effective classification outcomes.Additionally,the recent advancements of deep learning(DL)models make it possible in several application areas.In addition,the performance of the DL models is mainly based on the hyperparameter setting which can be resolved by the design of metaheuristics.In this view,this article develops an automated red deer algorithm with deep learning enabled hyperspec-tral image(HSI)classification(RDADL-HIC)technique.The proposed RDADL-HIC technique aims to effectively determine the HSI images.In addition,the RDADL-HIC technique comprises a NASNetLarge model with Adagrad optimi-zer.Moreover,RDA with gated recurrent unit(GRU)approach is used for the identification and classification of HSIs.The design of Adagrad optimizer with RDA helps to optimally tune the hyperparameters of the NASNetLarge and GRU models respectively.The experimental results stated the supremacy of the RDADL-HIC model and the results are inspected interms of different measures.The comparison study of the RDADL-HIC model demonstrated the enhanced per-formance over its recent state of art approaches.
基金We acknowledge Taif University for Supporting this study through Taif University Researchers Supporting Project number(TURSP-2020/173)Taif University,Taif,Saudi Arabia.
文摘Sentiment Analysis(SA)is one of the subfields in Natural Language Processing(NLP)which focuses on identification and extraction of opinions that exist in the text provided across reviews,social media,blogs,news,and so on.SA has the ability to handle the drastically-increasing unstructured text by transform-ing them into structured data with the help of NLP and open source tools.The current research work designs a novel Modified Red Deer Algorithm(MRDA)Extreme Learning Machine Sparse Autoencoder(ELMSAE)model for SA and classification.The proposed MRDA-ELMSAE technique initially performs pre-processing to transform the data into a compatible format.Moreover,TF-IDF vec-torizer is employed in the extraction of features while ELMSAE model is applied in the classification of sentiments.Furthermore,optimal parameter tuning is done for ELMSAE model using MRDA technique.A wide range of simulation analyses was carried out and results from comparative analysis establish the enhanced effi-ciency of MRDA-ELMSAE technique against other recent techniques.
文摘Minerals are critical in maintaining health and physiological function in wildlife. Geographic variation in soil and forage mineral concentration may predispose wildlife to mineral imbalances, where a common symptom is restricted somatic growth. We investigated if mineral imbalances could explain localized differences in morphology of white-tailed deer (Odocoileus virginianus) occurring in geographically proximate sites with similar management, climate, and habitat. We collected serum samples and morphological measurements from free-ranging white-tailed deer captured during 2011-2019 from coastal and inland rangeland sites in South Texas, USA. We measured mineral concentrations in serum from captured deer at each location. Asymptotic deer body mass and antler size averaged 8% - 20% smaller for deer at the coastal compared to the inland site. The proportion of deer with deficient levels of serum copper was greater at the coastal site (66% versus 14%). Our results suggest regional mineral deficiencies in deer may limit antler and body development. Wildlife managers should be aware of all aspects of wildlife nutrition and the importance of considering nutrients beyond energy and protein.
基金This work received supportsfrom Natural Science Foundation of Yun nan Science Technology Committee granted to SuBingpartlys
文摘Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies.
文摘A study was conducted on the identifications of the degraded samples of sika deer (Cervus nippon) and red deer (Cervus elaphus) by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical (0.026±0.006), which was smaller than the smallest nucleotide distance between eastern red deer and sika deer (0.036). Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.
基金Supported by Youth Foundation of Education Department in Sichuan Province (07ZB060)Youth Science and Technology Innovation Fund in Sichuan Agricultural University~~
文摘[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.
文摘Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.