针对DHCR和DR(direct hot charging rolling and direct rolling)工艺的特点,提出了一种包括批量计划层、作业调度层和动态调度层的DHCR和DR一体化计划调度三层体系结构。批量计划层包括合同计划优化模块和一体化批量计划优化模块,作业...针对DHCR和DR(direct hot charging rolling and direct rolling)工艺的特点,提出了一种包括批量计划层、作业调度层和动态调度层的DHCR和DR一体化计划调度三层体系结构。批量计划层包括合同计划优化模块和一体化批量计划优化模块,作业调度层包括作业调度优化模块,动态调度层包括基于规则的动态调度主控模块和实时控制器。分析了各层的结构、功能、特点和若干设计指导思想。按照该体系结构开发了武钢二热轧DHCR生产管理系统,生产计划的75%可实现直装轧制(平均装炉温度在700℃以上),加热炉产能提高了30%,同时减少了计划编制和调度所需要的时间。实际应用结果表明,该体系结构是行之有效的。展开更多
24-脱氢胆固醇还原酶（3β-hydroxysterolΔ~24-reductase,DHCR24）可催化链甾醇转化为胆固醇,参与多种脂代谢异常相关疾病（肝病、心血管疾病和链甾醇血症等）的发生发展。丙型肝炎病毒（hepatitis C virus,HCV）感染导致肝脏脂代谢紊...24-脱氢胆固醇还原酶（3β-hydroxysterolΔ~24-reductase,DHCR24）可催化链甾醇转化为胆固醇,参与多种脂代谢异常相关疾病（肝病、心血管疾病和链甾醇血症等）的发生发展。丙型肝炎病毒（hepatitis C virus,HCV）感染导致肝脏脂代谢紊乱以及肝脏炎症,并且可通过增强子诱导DHCR24表达增多[1-2]。展开更多
Background: 24-dehydrocholesterol reductase(Dhcr24) catalyzes the last step of cholesterol biosynthesis, which is required for normal development and antiapoptotic activities of tissues. We found that Dhcr24 expressio...Background: 24-dehydrocholesterol reductase(Dhcr24) catalyzes the last step of cholesterol biosynthesis, which is required for normal development and antiapoptotic activities of tissues. We found that Dhcr24 expression decreased in the cTnTR141 Wdilated cardiomyopathy(DCM) transgenic mice. Therefore, we tested whether rescued expression of Dhcr24 could prevent the development of DCM and its possible mechanism.Methods: Heart tissue specific transgenic overexpression mice of Dhcr24 was generated, then was crossed to c TnTR141 Wmouse to obtain the double transgenic mouse(DTG). The phenotypes were demonstrated by the survival, cardiac geometry and function analysis, as well as microstructural and ultrastructural observations based on echocardiography and histology examination. The pathway and apoptosis were analysed by western blotting and TUNEL assay in vivo and in vitro.Results: We find that Dhcr24 decreased in hearts tissues of cTnTR141 Wand LMNAE82 KDCM mice. The transgenic overexpression of Dhcr24 significantly improves DCM phenotypes in cTnTR141 Wmice, and activates PI3K/Akt/HKII pathway, followed by a reduction of the translocation of Bax and release of cytochrome c, caspase-9 and caspase-3 activation and myocyte apoptosis. Knockdown the expression of Dhcr24 reduces the activation of PI3K/Akt/HKII pathway and inhibition of the mitochondrial-dependent apoptosis. The anti-apoptotic effect of Dhcr24 could be completely removed by the inhibition of PI3K pathway and partly removed by the HKII inhibitor in H9c2 cell line.Conclusion: Compensatory expression of Dhcr24 protect against DCM through activated PI3K/Akt/HKII pathway and reduce Bax translocation. This is the first investigation for the molecular mechanism of Dhcr24 participate in development of DCM.展开更多
随着宝钢分公司十一五项目陆续投产,公司的能源平衡受到了严峻考验。DHCR计划(Direct Hot Charge Rolling直接热装计划)作为一种不改变现有工艺、设备状况条件下的生产组织方法,能够有效地兼顾热轧工序产能提升和节能降耗双重需求。但由...随着宝钢分公司十一五项目陆续投产,公司的能源平衡受到了严峻考验。DHCR计划(Direct Hot Charge Rolling直接热装计划)作为一种不改变现有工艺、设备状况条件下的生产组织方法,能够有效地兼顾热轧工序产能提升和节能降耗双重需求。但由于DHCR因为钢种单一且受合同结构的影响,计划单重通常较小。如果单纯地提高DHCR计划数量必将导致计划单重明显下降进而引起热轧辊耗上升,这种情况持续下去可能在热轧工序产生"节能不降耗"的奇怪现象。只有同时提高DHCR的计划单重才可以把DHCR的优势充分发挥出来。本文以DHCR计划实施入手,对影响DHCR计划单重的因素进行分析并提出相应的解决办法。展开更多
This paper presented the application of the direct hot charge rolling （DHCR） technology in Ningbo Steel. Five aspects have been systematically addressed and analyzed, which include sales order, production schedule p...This paper presented the application of the direct hot charge rolling （DHCR） technology in Ningbo Steel. Five aspects have been systematically addressed and analyzed, which include sales order, production schedule programming, steelmaking,continuous casting and hot-rolling. The average DHCR product rate has reached 55% and the average slab charging temperature 699℃. As a result, the production cost, the energy consumption and the CO2emission have been significantly reduced. It is concluded that a breakthrough in application of the DHCR technology has been achieved at Ningbo Steel.展开更多
Zika virus(ZIKV)evolves non-structural proteins to evade immune response and ensure efficient replication in the host cells.Cholesterol metabolic enzyme 7-dehydrocholesterol reductase(DHCR7)was recently reported to im...Zika virus(ZIKV)evolves non-structural proteins to evade immune response and ensure efficient replication in the host cells.Cholesterol metabolic enzyme 7-dehydrocholesterol reductase(DHCR7)was recently reported to impact innate immune responses in ZIKV infection.However,the vital non-structural protein and mechanisms involved in DHCR7-mediated viral evasion are not well elucidated.In this study,we demonstrated that ZIKV infection facilitated DHCR7 expression.Notably,the upregulated DHCR7 in turn facilitated ZIKV infection and blocking DHCR7 suppressed ZIKV infection.Mechanically,ZIKV non-structural protein 4B(NS4B)interacted with DHCR7 to induce DHCR7 expression.Moreover,DHCR7 inhibited TANK-binding kinase 1(TBK1)and interferon regulatory factor 3(IRF3)phosphorylation,which resulted in the reduction of interferon-beta(IFN-β)and interferon-stimulated genes(ISGs)productions.Therefore,we propose that ZIKV NS4B binds to DHCR7 to repress TBK1 and IRF3 activation,which in turn inhibits IFN-βand ISGs,and thereby facilitating ZIKV evasion.This study broadens the insights on how viral non-structural proteins antagonize innate immunity to facilitate viral infection via cholesterol metabolic enzymes and intermediates.展开更多
文摘针对DHCR和DR(direct hot charging rolling and direct rolling)工艺的特点,提出了一种包括批量计划层、作业调度层和动态调度层的DHCR和DR一体化计划调度三层体系结构。批量计划层包括合同计划优化模块和一体化批量计划优化模块,作业调度层包括作业调度优化模块,动态调度层包括基于规则的动态调度主控模块和实时控制器。分析了各层的结构、功能、特点和若干设计指导思想。按照该体系结构开发了武钢二热轧DHCR生产管理系统,生产计划的75%可实现直装轧制(平均装炉温度在700℃以上),加热炉产能提高了30%,同时减少了计划编制和调度所需要的时间。实际应用结果表明,该体系结构是行之有效的。
文摘24-脱氢胆固醇还原酶（3β-hydroxysterolΔ~24-reductase,DHCR24）可催化链甾醇转化为胆固醇,参与多种脂代谢异常相关疾病（肝病、心血管疾病和链甾醇血症等）的发生发展。丙型肝炎病毒（hepatitis C virus,HCV）感染导致肝脏脂代谢紊乱以及肝脏炎症,并且可通过增强子诱导DHCR24表达增多[1-2]。
基金The present work was supported by the National Science and Technology Support Project(2015BAI08B01)CAMS Innovation Fund for Medical Sciences(CAMS-I2M,2016-I2M-1-015)Beijing Natural Science Foundation(5172027)
文摘Background: 24-dehydrocholesterol reductase(Dhcr24) catalyzes the last step of cholesterol biosynthesis, which is required for normal development and antiapoptotic activities of tissues. We found that Dhcr24 expression decreased in the cTnTR141 Wdilated cardiomyopathy(DCM) transgenic mice. Therefore, we tested whether rescued expression of Dhcr24 could prevent the development of DCM and its possible mechanism.Methods: Heart tissue specific transgenic overexpression mice of Dhcr24 was generated, then was crossed to c TnTR141 Wmouse to obtain the double transgenic mouse(DTG). The phenotypes were demonstrated by the survival, cardiac geometry and function analysis, as well as microstructural and ultrastructural observations based on echocardiography and histology examination. The pathway and apoptosis were analysed by western blotting and TUNEL assay in vivo and in vitro.Results: We find that Dhcr24 decreased in hearts tissues of cTnTR141 Wand LMNAE82 KDCM mice. The transgenic overexpression of Dhcr24 significantly improves DCM phenotypes in cTnTR141 Wmice, and activates PI3K/Akt/HKII pathway, followed by a reduction of the translocation of Bax and release of cytochrome c, caspase-9 and caspase-3 activation and myocyte apoptosis. Knockdown the expression of Dhcr24 reduces the activation of PI3K/Akt/HKII pathway and inhibition of the mitochondrial-dependent apoptosis. The anti-apoptotic effect of Dhcr24 could be completely removed by the inhibition of PI3K pathway and partly removed by the HKII inhibitor in H9c2 cell line.Conclusion: Compensatory expression of Dhcr24 protect against DCM through activated PI3K/Akt/HKII pathway and reduce Bax translocation. This is the first investigation for the molecular mechanism of Dhcr24 participate in development of DCM.
文摘随着宝钢分公司十一五项目陆续投产,公司的能源平衡受到了严峻考验。DHCR计划(Direct Hot Charge Rolling直接热装计划)作为一种不改变现有工艺、设备状况条件下的生产组织方法,能够有效地兼顾热轧工序产能提升和节能降耗双重需求。但由于DHCR因为钢种单一且受合同结构的影响,计划单重通常较小。如果单纯地提高DHCR计划数量必将导致计划单重明显下降进而引起热轧辊耗上升,这种情况持续下去可能在热轧工序产生"节能不降耗"的奇怪现象。只有同时提高DHCR的计划单重才可以把DHCR的优势充分发挥出来。本文以DHCR计划实施入手,对影响DHCR计划单重的因素进行分析并提出相应的解决办法。
文摘This paper presented the application of the direct hot charge rolling （DHCR） technology in Ningbo Steel. Five aspects have been systematically addressed and analyzed, which include sales order, production schedule programming, steelmaking,continuous casting and hot-rolling. The average DHCR product rate has reached 55% and the average slab charging temperature 699℃. As a result, the production cost, the energy consumption and the CO2emission have been significantly reduced. It is concluded that a breakthrough in application of the DHCR technology has been achieved at Ningbo Steel.
基金supported by the National Natural Science Foundation of China(81730061,81802008)the Guangdong Basic and Applied Basic Research Foundation(2021A1515011272).
文摘Zika virus(ZIKV)evolves non-structural proteins to evade immune response and ensure efficient replication in the host cells.Cholesterol metabolic enzyme 7-dehydrocholesterol reductase(DHCR7)was recently reported to impact innate immune responses in ZIKV infection.However,the vital non-structural protein and mechanisms involved in DHCR7-mediated viral evasion are not well elucidated.In this study,we demonstrated that ZIKV infection facilitated DHCR7 expression.Notably,the upregulated DHCR7 in turn facilitated ZIKV infection and blocking DHCR7 suppressed ZIKV infection.Mechanically,ZIKV non-structural protein 4B(NS4B)interacted with DHCR7 to induce DHCR7 expression.Moreover,DHCR7 inhibited TANK-binding kinase 1(TBK1)and interferon regulatory factor 3(IRF3)phosphorylation,which resulted in the reduction of interferon-beta(IFN-β)and interferon-stimulated genes(ISGs)productions.Therefore,we propose that ZIKV NS4B binds to DHCR7 to repress TBK1 and IRF3 activation,which in turn inhibits IFN-βand ISGs,and thereby facilitating ZIKV evasion.This study broadens the insights on how viral non-structural proteins antagonize innate immunity to facilitate viral infection via cholesterol metabolic enzymes and intermediates.
