Myoblast differentiation is an essential process during skeletal muscle development.C2C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro.Dehydrogenase/reductase(SDR family...Myoblast differentiation is an essential process during skeletal muscle development.C2C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro.Dehydrogenase/reductase(SDR family)member 3(DHRS3)is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of retinol.Previous experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts differentiation.However,the effect of DHRS3 on mouse muscle cell differentiation was unclear.The objective of current study was to determine if DHRS3 affected muscle cell differentiation,and if DHRS3 was involved in muscle regeneration.Protein expression was determined by western blot and immunofluorescence analysis.The activation and inhibition of DHRS3 increased and decreased C2C12 myoblast differentiation respectively,which indicated that DHRS3 could affect C2C12 myoblast differentiation.DHRS3 expression was significantly changed during muscle regeneration,with the regeneration of muscle injury,the expression of DHRS3 tended to increase first and then decrease.It suggested that DHRS3 might be involved in muscle regeneration.In summary,this study confirmed the involvement of DHRS3 in C2C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development.展开更多
For a long time,it has been argued that the theories and practices devoted to urban planning and management should conform to the fundamental role of planning policies in the production of urban space,but not merely t...For a long time,it has been argued that the theories and practices devoted to urban planning and management should conform to the fundamental role of planning policies in the production of urban space,but not merely the spatial distribution of the produced services.Towards this wider connotation,this study introduces the notion of Railwayscape,grounded on the theory of‘The Production of Space’,to examine the role of railway station districts as catalysts of urban development through the social production of urban space.The present research sets out to establish the notion of Railwayscape and apply it in a railway heritage,i.e.,the Darjeeling Himalayan Railway(DHR)and its point of inception,Siliguri City,India.Accordingly,a criteria-based evaluation of four railway station districts(New Jalpaiguri,Siliguri Town,Siliguri Junction,and Sukna)in Siliguri and its surroundings was performed.The information regarding the selected four railway station districts is obtained through field observation and key informant consultation,supplemented by published literature and remote sensing data.This evaluation is succeeded by the strengths,weaknesses,opportunities and threats(SWOT)analysis accentuating the potential strengths,weaknesses,opportunities and threats associated with the selected four railway station districts and their prospects to become the potential Railwayscape.The results of this research show that there is no railway station district in Siliguri that can fully meet the demands of the locals and tourists,therefore,relfecting a lack of awareness of the historical values of these districts.The results also indicate that there are significant differences in the relative potentials of railway station districts to become the Railwayscape in urban environment.The outcomes of this research,therefore,are expected to encourage policy-making insitutions and practitioners to realise the‘place value’of some railway station districts and their potentials to yield better economic,social and structural virtue for a wide range of actors.展开更多
基金Supported by National Natural Science Foundation of China (No. 31100943 )Shenzhen Nanshan Science and Technology Research Fund (No.2010012)Shenzhen City Science and Technology Project (No.201102136) ~~
基金Supported by the Natural Science Foundation of Heilongjiang Province(C2017025)。
文摘Myoblast differentiation is an essential process during skeletal muscle development.C2C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro.Dehydrogenase/reductase(SDR family)member 3(DHRS3)is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of retinol.Previous experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts differentiation.However,the effect of DHRS3 on mouse muscle cell differentiation was unclear.The objective of current study was to determine if DHRS3 affected muscle cell differentiation,and if DHRS3 was involved in muscle regeneration.Protein expression was determined by western blot and immunofluorescence analysis.The activation and inhibition of DHRS3 increased and decreased C2C12 myoblast differentiation respectively,which indicated that DHRS3 could affect C2C12 myoblast differentiation.DHRS3 expression was significantly changed during muscle regeneration,with the regeneration of muscle injury,the expression of DHRS3 tended to increase first and then decrease.It suggested that DHRS3 might be involved in muscle regeneration.In summary,this study confirmed the involvement of DHRS3 in C2C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development.
基金University Grants Commission for the Junior Research Fellowship(Ref No.190510006296).
文摘For a long time,it has been argued that the theories and practices devoted to urban planning and management should conform to the fundamental role of planning policies in the production of urban space,but not merely the spatial distribution of the produced services.Towards this wider connotation,this study introduces the notion of Railwayscape,grounded on the theory of‘The Production of Space’,to examine the role of railway station districts as catalysts of urban development through the social production of urban space.The present research sets out to establish the notion of Railwayscape and apply it in a railway heritage,i.e.,the Darjeeling Himalayan Railway(DHR)and its point of inception,Siliguri City,India.Accordingly,a criteria-based evaluation of four railway station districts(New Jalpaiguri,Siliguri Town,Siliguri Junction,and Sukna)in Siliguri and its surroundings was performed.The information regarding the selected four railway station districts is obtained through field observation and key informant consultation,supplemented by published literature and remote sensing data.This evaluation is succeeded by the strengths,weaknesses,opportunities and threats(SWOT)analysis accentuating the potential strengths,weaknesses,opportunities and threats associated with the selected four railway station districts and their prospects to become the potential Railwayscape.The results of this research show that there is no railway station district in Siliguri that can fully meet the demands of the locals and tourists,therefore,relfecting a lack of awareness of the historical values of these districts.The results also indicate that there are significant differences in the relative potentials of railway station districts to become the Railwayscape in urban environment.The outcomes of this research,therefore,are expected to encourage policy-making insitutions and practitioners to realise the‘place value’of some railway station districts and their potentials to yield better economic,social and structural virtue for a wide range of actors.
