It is well known that the function of membrane protein is closely related to its own structure. Besides, there is also connection of membrane protein with its surrounding lipids, other membrane proteins, and the cytos...It is well known that the function of membrane protein is closely related to its own structure. Besides, there is also connection of membrane protein with its surrounding lipids, other membrane proteins, and the cytosolic components in the living cells. Such interaction is often important to performance and regulation of its normal physiological function. In this note, by using a specific inhibitor of anion transport protein (Band 3), DIDS (4,4’-diisothiocyno-stilbene-2,2’-disulfonate), we have studied whether or not it could also affect the glucose transport property of glucose transport protein (GluT-1) in the red cell membranes. By this way we want to confirm whether there is any interaction between these two kinds of membrane proteins in the membranes, by which the function of mem-brane protein would be regulated.展开更多
In order to learn the ways and possible utilization mechanisms of dissolved inorganic carbon (DIC) in marine phytoplankton species under carbon-replete or -limited conditions, the activity of extracellular carbonic an...In order to learn the ways and possible utilization mechanisms of dissolved inorganic carbon (DIC) in marine phytoplankton species under carbon-replete or -limited conditions, the activity of extracellular carbonic anhydrase (CA) was assayed in different pH, CO 2 and DIC concentrations. Extracellular CA in Amphidinium carterae and Prorocentrum minimum was detected under carbon-replete conditions, while in Melosira sp., Phaeodactylum tricornutum, Skeletonema costatum, Thalassiosira rotula, Emiliania huxleyi and Pleurochrysis carterae, CA activity was assayed under conditions of carbon limitation. No CA activity was found even under carbon-limited conditions in Chaetoceros compressus, Glenodinium foliaceum, Coccolithus pelagicus, Gephrocapsa oceanica and Heterosigma akashiwo. In species without extracellular CA activity, the direct HCO - 3 uptake was investigated using a pH drift technique and the anion exchange inhibitor 4′4′-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS) in a closed system. The result showed that direct HCO - 3 transport might occur by an anion exchange mechanism in species Coc. pelagicus and G. oceanica. Of the 13 species investigated, only H. akashiwo did not have the potential for direct uptake or extracellular CA-catalyzed HCO - 3 utilization.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘It is well known that the function of membrane protein is closely related to its own structure. Besides, there is also connection of membrane protein with its surrounding lipids, other membrane proteins, and the cytosolic components in the living cells. Such interaction is often important to performance and regulation of its normal physiological function. In this note, by using a specific inhibitor of anion transport protein (Band 3), DIDS (4,4’-diisothiocyno-stilbene-2,2’-disulfonate), we have studied whether or not it could also affect the glucose transport property of glucose transport protein (GluT-1) in the red cell membranes. By this way we want to confirm whether there is any interaction between these two kinds of membrane proteins in the membranes, by which the function of mem-brane protein would be regulated.
文摘In order to learn the ways and possible utilization mechanisms of dissolved inorganic carbon (DIC) in marine phytoplankton species under carbon-replete or -limited conditions, the activity of extracellular carbonic anhydrase (CA) was assayed in different pH, CO 2 and DIC concentrations. Extracellular CA in Amphidinium carterae and Prorocentrum minimum was detected under carbon-replete conditions, while in Melosira sp., Phaeodactylum tricornutum, Skeletonema costatum, Thalassiosira rotula, Emiliania huxleyi and Pleurochrysis carterae, CA activity was assayed under conditions of carbon limitation. No CA activity was found even under carbon-limited conditions in Chaetoceros compressus, Glenodinium foliaceum, Coccolithus pelagicus, Gephrocapsa oceanica and Heterosigma akashiwo. In species without extracellular CA activity, the direct HCO - 3 uptake was investigated using a pH drift technique and the anion exchange inhibitor 4′4′-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS) in a closed system. The result showed that direct HCO - 3 transport might occur by an anion exchange mechanism in species Coc. pelagicus and G. oceanica. Of the 13 species investigated, only H. akashiwo did not have the potential for direct uptake or extracellular CA-catalyzed HCO - 3 utilization.
