Background: Renal osteodystrophy (ROD) is a bone disorder resulting from chronic kidney disease (CKD) and related metabolic diseases. Dickkopf-related protein-1 (DKK-1) is critical in regulating bone biology. This stu...Background: Renal osteodystrophy (ROD) is a bone disorder resulting from chronic kidney disease (CKD) and related metabolic diseases. Dickkopf-related protein-1 (DKK-1) is critical in regulating bone biology. This study aimed to evaluate the serum DKK-1 level as a bone marker in children with CKD who undergo regular hemodialysis (HD). Subjects and Methods: This case-control study involved 40 children with CKD on HD and 40 healthy children as controls. The study measured serum DKK-1 levels and performed a dual-energy X-ray absorptiometry scan (DEXA) in line with routine laboratory investigations. Results: There was a significant increase in the serum level of DKK-1 in the patient group compared to the control group. The DKK-1 levels were 2540.65 (2215.4 - 2909.2) pg/ml and 1110.45 (885.45 - 1527.65) pg/ml, respectively, with a p-value of less than 0.001. In the hemodialysis group, 25 patients (62.5%) had low bone mineral density (BMD) with a Z-score of under -2.0. Eighteen of these patients had low BMD in both the neck of the femur and lumbar spines. Additionally, there was a significant increase in serum DKK-1 level in patients with low BMD (2567.35 (2303.8 - 3108.1) pg/ml) compared to patients with normal BMD (2454 (1859 - 2820) pg/ml) (p = 0.041). There was also a significant positive correlation between DKK1 level and phosphorus, alkaline phosphatase, and Parathormone serum levels. In conclusion, the study indicates a clear correlation between DKK-1 and BMD in children undergoing maintenance hemodialysis. DKK1 is a promising biomarker for CKD-MBD.展开更多
背景与目的肺癌是我国最常见的恶性肿瘤,肺腺癌(lung adenocarcinoma,LUAD)是肺癌的主要类型,严重威胁着人民的生命健康,目前关于血清分泌型蛋白1(Dikkopf 1,DKK1)在LUAD中的作用研究较少,本研究旨在通过生物信息学方法探究DKK1在LUAD...背景与目的肺癌是我国最常见的恶性肿瘤,肺腺癌(lung adenocarcinoma,LUAD)是肺癌的主要类型,严重威胁着人民的生命健康,目前关于血清分泌型蛋白1(Dikkopf 1,DKK1)在LUAD中的作用研究较少,本研究旨在通过生物信息学方法探究DKK1在LUAD发生发展中的作用及潜在的预后价值。方法应用基因型组织表达(genotype-tissue expression,GTEx)、癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库和肿瘤与免疫系统交互网站(tumor-immune system interactions database,TISIDB)等多个数据库,对DKK1在LUAD中的表达、临床病理特征、免疫细胞浸润、预后和甲基化等进行分析,同时应用LinkedOmics数据库分析DKK1的共表达基因及其功能富集。收集2016年至2017年于新疆医科大学附属肿瘤医院行手术治疗的59例石蜡包埋的LUAD患者病理样本,通过免疫组织化学试验(immunohistochemistry,IHC)进行表达预后验证。结果生信分析结果显示DKK1在LUAD组织的表达水平高于正常组织,晚期癌症中的表达高于早期阶段,实验验证后发现59例LUAD中阴性表达15例(25.4%),弱阳性表达18例(30.5%),强阳性表达26例(44.1%)。DKK1的不同表达情况与甲基化、预后以及多种免疫细胞的活动相关。功能富集显示DKK1可能参与表皮发育、细胞-基质连接等过程,京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析表明DKK1与ABC转运蛋白相关。生物信息学分析及临床病例标本显示DKK1高表达与LUAD患者较差的预后有关。结论DKK1在LUAD中高表达,与患者预后不良有关,并且DKK1与肿瘤免疫细胞浸润和通路密切相关。DKK1可能是LUAD潜在的预后标志物和免疫治疗新靶点。展开更多
文摘Background: Renal osteodystrophy (ROD) is a bone disorder resulting from chronic kidney disease (CKD) and related metabolic diseases. Dickkopf-related protein-1 (DKK-1) is critical in regulating bone biology. This study aimed to evaluate the serum DKK-1 level as a bone marker in children with CKD who undergo regular hemodialysis (HD). Subjects and Methods: This case-control study involved 40 children with CKD on HD and 40 healthy children as controls. The study measured serum DKK-1 levels and performed a dual-energy X-ray absorptiometry scan (DEXA) in line with routine laboratory investigations. Results: There was a significant increase in the serum level of DKK-1 in the patient group compared to the control group. The DKK-1 levels were 2540.65 (2215.4 - 2909.2) pg/ml and 1110.45 (885.45 - 1527.65) pg/ml, respectively, with a p-value of less than 0.001. In the hemodialysis group, 25 patients (62.5%) had low bone mineral density (BMD) with a Z-score of under -2.0. Eighteen of these patients had low BMD in both the neck of the femur and lumbar spines. Additionally, there was a significant increase in serum DKK-1 level in patients with low BMD (2567.35 (2303.8 - 3108.1) pg/ml) compared to patients with normal BMD (2454 (1859 - 2820) pg/ml) (p = 0.041). There was also a significant positive correlation between DKK1 level and phosphorus, alkaline phosphatase, and Parathormone serum levels. In conclusion, the study indicates a clear correlation between DKK-1 and BMD in children undergoing maintenance hemodialysis. DKK1 is a promising biomarker for CKD-MBD.
文摘背景与目的肺癌是我国最常见的恶性肿瘤,肺腺癌(lung adenocarcinoma,LUAD)是肺癌的主要类型,严重威胁着人民的生命健康,目前关于血清分泌型蛋白1(Dikkopf 1,DKK1)在LUAD中的作用研究较少,本研究旨在通过生物信息学方法探究DKK1在LUAD发生发展中的作用及潜在的预后价值。方法应用基因型组织表达(genotype-tissue expression,GTEx)、癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库和肿瘤与免疫系统交互网站(tumor-immune system interactions database,TISIDB)等多个数据库,对DKK1在LUAD中的表达、临床病理特征、免疫细胞浸润、预后和甲基化等进行分析,同时应用LinkedOmics数据库分析DKK1的共表达基因及其功能富集。收集2016年至2017年于新疆医科大学附属肿瘤医院行手术治疗的59例石蜡包埋的LUAD患者病理样本,通过免疫组织化学试验(immunohistochemistry,IHC)进行表达预后验证。结果生信分析结果显示DKK1在LUAD组织的表达水平高于正常组织,晚期癌症中的表达高于早期阶段,实验验证后发现59例LUAD中阴性表达15例(25.4%),弱阳性表达18例(30.5%),强阳性表达26例(44.1%)。DKK1的不同表达情况与甲基化、预后以及多种免疫细胞的活动相关。功能富集显示DKK1可能参与表皮发育、细胞-基质连接等过程,京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析表明DKK1与ABC转运蛋白相关。生物信息学分析及临床病例标本显示DKK1高表达与LUAD患者较差的预后有关。结论DKK1在LUAD中高表达,与患者预后不良有关,并且DKK1与肿瘤免疫细胞浸润和通路密切相关。DKK1可能是LUAD潜在的预后标志物和免疫治疗新靶点。
