Relationship Between Grain Yield and Yield Components in Super Hybrid Rice

Chinese super hybrid rice breeding project has developed many new varieties with great yield potential. It is controversial which yield component should be emphasized in super hybrid rice production. The present study was conducted to compare super hybrid rice with common hybrid and super inbred rice and analyze contributions of yield components to grain yield of super hybrid rice under experimental conditions, and evaluate relationships between grain yield and yield components of super hybrid rice in farmer’s paddy fields. Field experiments were done in Changsha, Guidong, and Nanxian, Hunan Province, China, from 2007 to 2009. Eight super hybrid varieties, one common hybrid variety, and one super inbred variety were grown in each location and year. Rice production investigation was undertaken in high-yielding （Guidong）, moderate-yielding （Nanxian）, and low-yielding （Ningxiang） regions of Hunan Province, China, in 2009. Grain yield and yield components were measured in both the field experiments and rice production investigation. Super hybrid rice varieties outyielded common hybrid and super inbred varieties across three locations and years. Yield potential has been increased by 11.4% in super hybrid rice varieties compared with common and super inbred varieties. The higher yield of super hybrid varieties was attributed to improvement in panicle size. Panicles per m2 had the highest positive contribution to grain yield with the exception under yield level of 10.0 to 12.0 t ha-1, and was positively related to grain yield in farmer’s field at all of the high-, moderate-, and low-yielding regions. Our study suggests that panicle per m2 ought to be emphasized in super hybrid rice production.

Practices and Prospects of Super Hybrid Rice Breeding

The great progress in super rice breeding both in China and other countries has been made in recent years. However, there were three main problems in super rice breeding： 1） the super rice varieties were still rare; 2） most super rice varieties exhibited narrow adaptability; and 3） current breeding theories emphasized too much on the rice growth model, but they were unpractical in guidance for rice breeding. According to the authors＇ experience on the rice breeding, the breeding strategies including three steps （super parent breeding, super hybrid rice breeding and super hybrid rice seed production） were proposed, and the objectives of each step and the key technologies to achieve the goals were elucidated in detail. The super parent of hybrid rice should exhibit excellent performance in all agronomic traits, with the yield or sink capacity reached the level of the hybrid rice control in regional trials. The super hybrid rice combination should meet the following criteria： good rice quality, wide adaptation, lodging resistance, resistance to main insects and diseases, and the yield exceeded above 8% over the control varieties in the national and provincial regional trials. To achieve the goal, the technical strategies, such as selecting optimal combination of the parents, increasing selection pressure, paying more attention to harmony of ideal plant type, excellent physiological traits and all the agronomic traits, should be emphasized. The yield of seed production should reach 3.75 t/ha and 5.25 t/ha for the super hybrid rice combinations derived from early-season and middle-season types of male sterile lines, respectively. The main technologies for raising seed production yield included selecting optimum seed production site, using the male sterile line with large sink capacity and good outcrossing characteristics, and improving the amount of the pollen by intensive cultivation of the male parent. According to the technologies of the three-step breeding on super hybrid rice, two super rice parents, including a male parent 996 and a thermo（photo）-genic male sterile [T（P）GMS] line C815S, were bred. Furthermore, a super early hybrid rice combination, Luliangyou 996, which could be used as a double-season early rice variety in middle and lower reaches of the Yangtze River, China, was bred by using the super rice varlet3, 996 as the male parent, and several hybrid rice combinations with higher yield than control variety in regional trials both of Hunan Province and state were bred with the T（P）GMS line C815S as the female parent.

Yield potential and stability in super hybrid rice and its production strategies

China＇s Super Hybrid Rice Breeding Program has made significant progress over the past two decades. In this paper, we reviewed our studies on the yield potential and stability in super hybrid rice and discussed the strategies for super hybrid rice production. The results of our studies show that rice yield potential has been increased by 12% in super hybrid cultivars as compared with ordinary hybrid and inbred cultivars. The higher grain yields in super hybrid rice cultivars are attributed to larger panicle size coupled with higher biomass production or higher harvest index. However, grain yields in super hybrid rice cultivars vary widely among locations depending on soil and climatic factors. Therefore, it is important to tailor target yield to local conditions in super hybrid rice production. The target yield for super hybrid rice production can be determined by the average yield method or the regression model method. Improving soil quality is critical to achieving the target yield in super hybrid rice production. Favorable crop rotations such as rice-oilseed rape and novel soil management practices, such as biochar addition, are effective approaches to improve soil quality. It is needed to develop simplified cultivation tech- nologies for super hybrid rice to meet the changes in socioeconomic environments during the period of transition. There are such technologies as no-tillage direct seeding and mechanized transplanting at high hill density with single seedling per hill.

Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers

Five super hybrid rice combinations, i.e. HYS-1/R105, Pei'ai 64S/E32, Liangyoupeijiu (Pei'ai 64S/9311), 88S/0293, and J23A/Q611, and their parental lines were tested by means of SSR analysis. A total of 144 SSR primer pairs distributed on 12 rice chromosomes were used, out of which 47 detected polymorphism among the tested rice lines. Among all these primers, RM337 and RM154 produced polymorphic patterns in four or more of the tested experimental materials respectively, and they could distinguish among most rice genotypes tested. Twenty-four primer pairs, two on each rice chromosome, were selected to make a reference SSR marker-based fingerprinting for the rice lines. For most of the primer pairs, F1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish the F1 from its parental lines. In addition, 5 primer pairs were selected as special primer pairs for five hybrid rice combinations respectively. By combining the rapid, simple method on DNA extraction, it is suggested that SSR technique has wide prospective in variety authentication and purity identification.

Yield and tillering response of super hybrid rice Liangyoupeijiu to tillage and establishment methods

Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of tillage(conventional and no-tillage system) and crop establishment methods(transplanting at a spacing of 20 cm × 20 cm with one seedling per hill and direct seeding at a seeding rate of 22.5 kg ha-1). Our results revealed that, at maximum tillering(Max.) and at maturity(MA) stages, direct seeding(DS) resulted in 22% more tillers than transplanting(TP) irrespective of tillage system. Tiller mortality reached a peak between panicle initiation(PI) and booting(BT) stages, and was 16% higher under conventional tillage(CT) than under no-tillage(NT). Transplanting required 29% more time for the completion of tillering and less for DS. Tillering rate was 43% higher in DS than TP under either CT or NT. There was a positive correlation between panicle number per m2and maximum tiller number per m2, but not panicle-bearing tiller rate. The panicle bearing tiller rate was higher under DS than TP and higher under NT than CT. Tiller dry weight gradually increased up to heading(HD) stage, and was 14% higher under TP than DS. Leaf area(cm2tiller-1) gradually increased from Max. to HD stage and then decreased by 34% in conventional tillage transplanting(CTTP) and 45% in no-tillage transplanting(NTTP) from 12DAH–24DAH(days after heading), but was similar(35%) under DS under either CT or NT. Grain yield was higher under CTTP owing to the larger sink size(heavier panicle, more spikelets in per cm length of panicle) than under DS.

Effects of Nitrogen Fertilizer Level on Chlorophyll Fluorescence Characteristics in Flag Leaf of Super Hybrid Rice at Late Growth Stage

To compare the effects of slow-release nitrogen fertilizer at six different levels on the flag leaf chlorophyll fluorescence characteristics of super hybrid rice, a field fertilization experiment was conducted with super hybrid rice Y Liangyou 1 as a test material. The photosynthetic electron transport rate （ETR）, effective quantum yield （EQY）, photochemical quenching coefficient （qp）, and non-photochemical quenching coefficient （NPQ） of flag leaves were measured at the initial heading, full heading, 10 d after full heading and 20 d after full heading stages. Results showed that the values of ETR, EQY and qp increased with rice development from initial heading to 20 d after full heading, whereas the NPQ decreased. During the measured stages, ETR, EQY and qp increased initially and then decreased as nitrogen application amount increased, but they peaked at different nitrogen fertilizer levels. The maximum ETR and EQY values appeared at the treatment of 135 kg/hm2 N. In conclusion, the optimum nitrogen amount for chlorophyll fluorescence characteristics of super hybrid rice was 135-180 kg/hm2.

Comparative Analysis on Rice Plant Type of Two Super Hybrids and Shanyou63

The characteristics of plant type and physiological indices for photosynthesis were analyzed using two super hybrid rices, Liangyoupeijiu and LiangyouE32 as materials, and an indica hybrid, Shan-you63 as a control. Based on the present analysis and various theories and breeding practices on rice plant type, a model of plant ideotype for super hybrid rice (indica) in the lower reaches of Yangtze River Valley was proposed. This was: a length of 35 - 40 cm for top leaf with a width of 2 cm, and that of 50 - 55 cm and 55-60 cm, respectively, for the second and third leaves from top, meanwhile, having a leaf angle of 5°, 10°and 15° respectively, and a curvature of 1 - 1. 5 cm-1for the leaf face at heading stage; the uppermost three leaves keeping their activities for as long as 70 d, which could lead a LAI of 3 at full ripeness; loose plant type with thin (SLW=2.5-3 g cm-2, dry weight) and curve-slant leaves during early growing stage, and compact plant type by thick (SLW=4 - 5 g cm-2) and erect leaves during the middle and late growing stages; with a coefficient of light extinction of 0. 3 - 0. 4, which allowed for an optimal LAI high of 8-10 during the middle growth period; plant height of 110-120 cm, with 2 - 4 cm basal internode and a long uppermost internode occupying 45% of total stem length; 25 - 28 cm panicle with 8-10 spikelets per centimeter showing bend-type in ripening; a rich chlorophyll which led to a high net photosynthetic rate, and tolerance to light shading and photooxidation which is of benefit to increasing the adaptability to varying light conditions.

Analysis on Dry Matter Production Characteristics of Super Hybrid Rice

Six middle-season indica hybrid rice combinations, including five super hybrid rice combinations with the high yield about 10.5 t/ha and a check hybrid rice combination Shanyou 63 with a yield potential about 9.5 t/ha, were used as materials to study the dry matter production characteristics. The super hybrid rice showed a high ability in dry matter production and accumulation and its yield enhanced with the increase of dry matter accumulation. The advantage period of dry matter production in the super hybrid rice was mainly at the middle and late growth stages compared with the check. The grain yield had no significant correlation with the dry matter accumulation before the elongation stage while had a significantly positive correlation with the dry matter accumulation from the elongation to maturity stages in super hybrid rice. There were more dry matter in vegetative organs at the heading stage in the super hybrid rice but its contribution to yield （apparent conversion percentage） was averagely 4.3 percent points lower than that in the check. For crop growth rate （CGR）, the comparative advantage of super hybrid rice was at the middle and late stages, especially after flowering. Moreover, as the rising of leaf area index （LAI） and leaf area duration （LAD）, CGR enhanced. The total LAD and the mean of lAD per day of super hybrid rice was about 14.79% and 10.31% higher than those of the check, respectively. The results indicate that the high yield of super hybrid rice mostly comes from the products of photosynthesis after heading, which is shown by the increased CGR at middle and later stages. It is suggested that LAD character might be used to better explain the advantage in the dry matter production of super hybrid rice than LAI.

Effect of Nitrogen Regimes on Grain Yield,Nitrogen Utilization,Radiation Use Efficiency,and Sheath Blight Disease Intensity in Super Hybrid Rice

Poor nitrogen use efficiency in rice production is a critical issue in China. Site-specific N managements （SSNM） such as real-time N management （RTNM） and fixed-time adjustable-dose N management （FTNM） improve fertilizer-N use efficiency of irrigated rice. This study was aimed to compare the different nitrogen （N） rates and application methods （FFP, SSNM, and RTNM methods） under with- and without-fungicide application conditions on grain yield, yield components, solar radiation use efficiency （RUE）, agronomic-nitrogen use efficiency （AEN）, and sheath blight disease intensity. Field experiments were carried out at Liuyang County, Hunan Province, China, during 2006 and 2007. A super hybrid rice Liangyou 293 （LY293） was used as experimental material. The results showed that RTNM and SSNM have great potential for improving agronomic-nitrogen use efficiency without sacrificing the grain yield. There were significant differences in light interception rate, sheath blight disease incidence （DI） and the disease index （ShBI）, and total dry matter among the different nitrogen management methods. The radiation use efficiency was increased in a certain level of applied N. But, the harvest index （HI） decreased with the increase in applied N. There is a quadratic curve relationship between grain yield and applied N rates. With the same N fertilizer rate, different fertilizer-N application methods affected the RUE and grain yield. The fungicide application not only improved the canopy light interception rate, RUE, grain filling, and harvest index, but also reduced the degree of sheath blight disease. The treatment of RTNM under the SPAD threshold value 40 obtained the highest yield. While the treatment of SSNM led to the highest nitrogen agronomic efficiency and higher rice yield, and decreased the infestation of sheath blight disease dramatically as well. Nitrogen application regimes and diseases control in rice caused obvious effects on light interception rate, RUE, and HI. Optimal N rate is helpful to get higher light interception rate, RUE, and HI. Disease control with fungicide application decreased and delayed the negative effects of the high N on rice yield formation. SSNM and RTNM under the proper SPAD threshold value obtained high-yield with high efficiency and could alleviate environmental pollution in rice production.

Voltage Stabilization of Hybrid Micro-Grid Using Super Capacitors

Scarcity of fossil fuel resources has motivated the researchers to develop renewable energy based power projects. Instead of using a single or independent renewable energy source, it is preferable to use the combination of such energy sources in a distributed way to compensate the power fluctuations of the system and this leads to the concept of hybrid micro-grid energy. Voltage stability is an important parameter for the secure operation of the hybrid-micro grid, and IEEE 1547 Standard defines the limit of the voltage for the successful operation of the micro-grid. Although Vanadium Redox Batteries (VRBs) can help the system to stabilize the voltage when voltage sag occurs when a heavy load is suddenly connected to the system, this stabilization process takes some time. This paper discusses the application of super capacitors to the hybrid micro-grid system, as a higher energy density element, to help the system quickly recover its transient voltage.

Effects of Plant Density and Nitrogen Application Rate on Grain Yield and Nitrogen Uptake of Super Hybrid Rice

The nitrogen uptake, yield and its components for two super-high-yielding hybrid rice combinations, Guodao 6 and Eryou 7954 were investigated under different plant densities （15, 18, and 21 plants/m^2） and different nitrogen application rates （120, 150, 180, and 210 kg/hm^2）. The experiment was conducted on loam soil during 2004-2006 at the experimental farm of the China National Rice Research Institute in Hangzhou, China. In these years, the two hybrid rice cleady showed higher yield at a plant density of 15 plants/m^2 with a nitrogen application rate of 180 kg/hm^2. Guodao 6 produced an average grain yield of 10 215.6 kg/hm^2 across the three years, while the yield of Eryou 7954 was 9 633.0 kg/hm^2. With fewer plants per unit-area and larger plants in the plots, the two hybrid rice produced more panicles per plant in three years. The highest nitrogen uptake of the two hybrid rice was at a plant density of 15 plants/m^2 with a nitrogen application rate of 180 kg/hm^2. Further increasing nitrogen application rate was not advantageous for nitrogen uptake in super-high-yielding rice under the same plant density.

Composite Electrodeposited PbO2/Co3O4 on a Ti Substrate as Positive Electrode Materials for a Hybrid Supercapacitor

PbO2/Co3O4 composites were prepared on a Ti substrate by means of a composite electrodeposition method in Pb2 plating solution containing dissolved nano-Co3O4 particles.X-ray diffraction(XRD),scanning electron microscopy(SEM),energy dispersive spectrometry(EDS)and transmission electron microscopy(TEM)were used to characterize the chemical composition and morphology of the PbCh/CogC^composites.The electrochemical and capacitanee performance of the composites were investigated by cyclic voltammetry(CV),charge-discharge tests and electrochemical impedance(EIS).The results indicate that the composites comprise rutile phase CO3O4 and 0?PbC)2?In addition,the surface of the composite electrode is rough and porous.The PbO2/Co3O4 composites exhibit a high specific capacitance up to 215 F/g,which is ten times higher than that of the pure-PbO?and two times higher than that of the pure-Co3O4 in 1 mol/L NaOH electrolytes.

Effects of Mutation and Hybridization on Breeding of Super High Yield Wheat Varieties

In order to develop high quality wheat varieties with super high yield, a theory which improved the genetic difference between parents by performing radi- ation mutation on them to enhance heterosis was proposed. The dry seeds treated and planted last year were radiated frequently by X- ray from 2003 to 2004. Then the dry seeds were radiated by high energy X ray combined with nuclear magnetic resonance from 2005 to 2007. We have treated the dry seeds repeatedly for 3 years and gained a variety of aberrant plants. These mutants were used as parents that were hybridized with the best plants in field. It was shown that a lot of mutant plants appeared in the f＇llial generations. After that, we selected big grains and super big grains among them. The result showed that, middle stem plants with big grains and low stem plants with big grains appeared. This phenomenon might be due to the breakthrough of linkage relationship between big grain trait and high stem trait. With the technique practice and materials, the new thoughts and methods were provided for induction and utilization of heterosis of wheat, which created a va- riety of seed selection and breeding materials for breeding super wheat varieties.

Human Face Super-Resolution Based on Hybrid Algorithm

Aiming at the problems of image super-resolution algorithm with many convolutional neural networks, such as large parameters, large computational complexity and blurred image texture, we propose a new algorithm model. The classical convolutional neural network is improved, the convolution kernel size is adjusted, and the parameters are reduced;the pooling layer is added to reduce the dimension. Reduced computational complexity, increased learning rate, and reduced training time. The iterative back-projection algorithm is combined with the convolutional neural network to create a new algorithm model. The experimental results show that compared with the traditional facial illusion method, the proposed method can obtain better performance.

Research on 48 V Super Capacitor Micro Hybrid System with 12 V Power Supply Multiplexing Function

48 V lithium battery micro hybrid system is the most fuel economy vehicle which can be mass produced at present.However,with the irreversible internal resistance increase of the key component 48 V lithium battery,and the capacity continues to decline,the system performance deteriorate.Worst case could be the system not functional in the middle and later age of vehicle life cycle.This paper studies the feasibility of using 48 V super capacitor as the replacement to 48 V lithium battery,and uses a 12 V module of 48 V super capacitor as the traditional 12 V power supply,further reducing the number of components or reducing the demand for parts of 48 V micro hybrid system.This paper analyses the 48 V super capacitor micro hybrid system scheme,based on which a prototype is built,and carries out the vehicle comparative test.The results show that:(1)The performance of 48 V super capacitor micro hybrid system perform comparably with 48 V lithium battery micro hybrid system,and 12 V multiplexing function does not cause power loss of super capacitor;(2)The SOC fluctuation of super capacitor is larger than that of lithium battery,but it can satisfy all test conditions through the strategy;(3)The voltage mutation of super capacitor is smaller than that of lithium battery.It can greatly reduce the impact of voltage on vehicle electrical appliances.The 48 V super capacitor micro hybrid system with 12 V multiplexing function is of great significance.

Below-diffraction-limited hybrid recording using silicon thin film super-resolution structure

We report on new experimental results for below-diffraction-limited hybrid recording. In our experiments, by means of focused laser assisted magnetic recording, the magnetic domains within TbFeCo thin films are obtained under an external perpendicular direct magnetic field. For a single magnetic medium, the domain size is mainly determined by the focused spot, which is about 620 nm for the laser wavelength λ=406 nm, and a numerical aperture of the lens of 0.80. However, when a silicon thin film structure is inserted between the substrate and the magnetic medium, the recording domains can be reduced obviously. By optimizing the experimental condition, even the size can be reduced to about 100 nm, which is below the diffraction limit, i.e. about 1/6 of the spot size. This is very useful for improving the hybrid recording density in practical applications.

Study on the Practice and High-yielding Mechanism of Super-sparse-cultivation Associated with Maximum-tiller Seedling of Hybrid Rice

In this paper, a new cultivation practice-super-sparse-cultivation associated with maximum-tiller seedling (SSCMTS) of hybrid rice was proposed and its high-yielding mechanism was studied. The results showed that the practice of SSCMTS in hybrid rice could not only increase grain yield but also save seeds and labor. It was a new high-yielding way for the late transplanting seedlings and heavy panicle type hybrid rice cultivars to further utilize the high-yielding potential of hybrid rice cultivars. The increasing number of spikelets and ideal grain -filling were the direct factors for the high yield of SSCMTS in hybrid rice, and those high-yielding factors relied on high quality seedlings, sturdy individuals, high quality population and vigorous later growth.

两种产量水平下超级杂交稻氮素吸收利用特性

探明不同产量水平下超级杂交稻的氮素吸收利用特性,可为我国西南稻区超级杂交稻高产栽培和育种提供理论和实践依据。以2个超级杂交稻品种(‘德优4727’‘泸优727’)和2个高产常规稻品种(‘金农丝苗’‘黄华占’)为材料,于2018—2020年在四川省德阳市(高产点)和泸州市(中产点)两个生态点进行大田和盆栽试验,研究两种产量水平下超级杂交稻氮素吸收、转运及利用效率的差异。结果表明:大田条件下不同生态点间超级杂交稻产量、氮素吸收积累利用特性差异显著。高产点超级杂交稻产量、氮肥偏生产力较中产点分别增加8.3%~23.2%、8.3%~23.1%。高产点超级杂交稻播种(SO)—幼穗分化(PI)、PI—齐穗(HD)阶段氮素吸收量和氮素吸收速率(除2018年幼穗分化—齐穗外)均高于中产点,HD—成熟(MA)阶段仍保持较高的氮素吸收量。高产点超级杂交稻成熟期氮素总吸收量较中产点增加15.6%~33.7%。尽管高产点超级杂交稻氮素收获指数较中产点平均增加4.6%(2018年除外),但成熟期仍有大量氮素滞留在超级杂交稻的秸秆中,造成高产点氮素籽粒生产效率较中产点平均减少11.3%。方差分析表明,盆栽条件下土壤、土壤×地点、土壤×品种互作对超级杂交稻产量、氮素吸收量、氮素籽粒生产效率影响不显著。高产点盆栽超级杂交稻产量、氮素吸收量、氮素籽粒生产效率变化趋势与大田试验相似。高产点SO—PI平均温度高于中产点,HD—MA平均温度低于中产点;高产点SO—PI、PI—HD和HD—MA太阳辐射积累量(除2018年PI-HD外)均高于中产点。相关分析表明,高产点籽粒产量与PI—HD氮素吸收量、氮素收获指数呈显著正相关;氮素籽粒生产效率与SO—PI的氮素吸收量和平均温度呈显著负相关。中产点籽粒产量与氮素籽粒生产效率、总吸氮量呈显著正相关;氮素籽粒生产效率与SO—PI和HD—MA的平均温度呈显著负相关。因此,不同产量水平超级杂交稻产量和氮素吸收利用特性差异主要与不同生育期内的平均温度和太阳辐射有关。