二甲烯丙基色氨酸合成酶的生物信息学分析

以二甲烯丙基色氨酸合成酶(DMATS)为研究对象,通过生物信息学方法共检索到37种微生物中的77条DMATS蛋白质序列。使用MEGA v4.0软件构建DMATS系统进化树发现,这些同源序列可分为两大簇,在第一簇中全为真菌的聚类,而第二簇中是细菌和真菌的聚类,初步分析可能是由于细菌和真菌的进化速度不同或DMATS基因是一个独立复制事件。在进行DMATS的信号肽结构预测时发现,只有Ajellomyces capsulata的DMATS(登录号:C6HC16)含有信号肽结构,由此可知,Ajellomyces capsulata(C6HC16)的DMATS可能是分泌蛋白质。通过使用MEME v4.9进行分析,最终得到了7条保守的motifs序列,这些motifs可能对DMATS发挥功能具有重要作用。

1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲的合成及荧光猝灭法测定海产品中的微量汞

合成了荧光试剂1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲(DMAT),其水溶液在448 nm处发射强荧光,通过研究DMAT在水溶液中对重金属离子的识别作用,发现DMAT对汞离子的识别具有高选择性、高灵敏性,且DMAT和汞(Ⅱ)形成配位比为1∶1的稳定络合物,产生荧光猝灭现象。据此建立了测定汞(Ⅱ)含量的1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲荧光分光光度法,其线性范围是0~9.9×10^-6 moL·L^-1,检出限为6.3×10^-8 moL·L^-1。该法用于海产品花蛤和虾头中微量汞的测定,方法简单,结果满意。

1，3－二甲基－6－氮杂胸腺嘧啶的C＝N双键与丙酮C＝O双键的光加成反应研究

以１，３－二甲基－６－氮杂胸腺嘧啶（ＤＭＡＴ）为底物，研究其５，６位的Ｃ＝Ｎ双键与丙酮Ｃ＝Ｏ双键的光加成反应，从反应产物中分离出了用热化学方法难以合成的氮、氧杂环丁烷的衍生物及其它副产物。该文亦对反应机理作了初步探讨。

Lessons from Partial Hospital Evacuations after the 2016 Central Tottori Earthquake

Objective: This study investigated the reasons, timing, procedures, and priorities of evacuations implemented by hospitals after the 2017 Central Tottori Earthquake to determine whether the evacuations were conducted appropriately. Methods: We collected patient and hospital data from the Disaster Medical Assistance Team (DMAT) coordination headquarters at the Prefectural Office. Requests for the transfer of 13 patients were analyzed. Results: The 13 patients were evacuated at night over seven hours, during which aftershocks occurred and falling debris was a high risk. We determined that none of the affected regions had emergency needs. Therefore, patient transport could have been conducted the following morning by bus and helicopter. Furthermore, patient transport could be efficiently carried out without physicians accompanying the patients. Nonetheless, consideration should be made regarding the handling of patient issues while in transit. Where a high number of patients are transported, hospitals would need to request the assistance of DMAT and Self-Defense Forces units earlier. Conclusions: Although all patients were successfully evacuated without incident with a few DMAT, It took for 7 hours to transfer 13 patients So hospitals should consider transporting patients during the day if there is no risk of building collapse. Hospitals should also prepare a plan for evacuation priority before the next disaster.