Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured ...Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured illumination can be obtained by either laser interference or projection of fringe patterns.Here,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM system.The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were studied.With the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil objectives.Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated.Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.展开更多
We present a digital micromirror device（DMD） based superpixel method for focusing light through scattering media by modulating the complex field of incident light. Firstly, we numerically and experimentally investig...We present a digital micromirror device（DMD） based superpixel method for focusing light through scattering media by modulating the complex field of incident light. Firstly, we numerically and experimentally investigate focusing light through a scattering sample using the superpixel methods with different target complex fields.Then, single-point and multiple-point focusing experiments are performed using this superpixel-based complex modulation method. In our experiment, up to 71.5% relative enhancement is realized. The use of the DMDbased superpixel method for the control of the complex field of incident light opens an avenue to improve the enhancement of focusing light through scattering media.展开更多
An ultrafast spectrum programmable femtosecond laser may enhance the performance of a wide variety of scientific applications,e.g.,multi-photon imaging.In this paper,we report a digital micromirror device(DMD)-based u...An ultrafast spectrum programmable femtosecond laser may enhance the performance of a wide variety of scientific applications,e.g.,multi-photon imaging.In this paper,we report a digital micromirror device(DMD)-based ultrafast pulse shaper,i.e.,DUPS,for femtosecond laser arbitrary amplitude shaping-the first time a programmable binary device reported to shape the amplitudes of ultrafast pulses spectrum at up to 32 kHz rate over a broad wavelength range.The DUPS is highly effcient,compact,and low cost based on the use of a DMD in combination with a transmission grating.Spatial and temporal dispersion introduced by the DUPS is compensated by a quasi-4-f setup and a grating pair,respectively.Femtosecond pulses with arbitrary spectrum shapes,including rectangular,sawtooth,triangular,double-pulse,and exponential profile,have been demonstrated in our experiments.A feedback operation process is implemented in the DUPS to ensure a robust and repeatable shaping process.The total effciency of the DUPS for amplitude shaping is measured to be 27%.展开更多
Laser shaping was introduced to maskless projection soft lithography by using digital micro-mirror device (DMD). The predesigned intensity pattern was imprinted onto the DMD and the input laser beam with a Gaussian or...Laser shaping was introduced to maskless projection soft lithography by using digital micro-mirror device (DMD). The predesigned intensity pattern was imprinted onto the DMD and the input laser beam with a Gaussian or quasi-Gaussian distribution will carry the pattern on DMD to etch the resin. It provides a method of precise control of laser beam shapes and?photon-induced curing behavior of resin. This technology provides an accurate micro-fabrication of microstructures used for micro-systems. As a virtual mask generator and a binary-amplitude spatial light modulator, DMD is equivalent to the masks in the conventional exposure system. As the virtual masks and shaped laser beam can be achieved flexibly, it is a good method of precision soft lithography for 2D/3D microstructures.展开更多
Structured illumination microscopy(SIM)achieves super-resolution(SR)by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction.The traditiona...Structured illumination microscopy(SIM)achieves super-resolution(SR)by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction.The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain,it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary,besides,the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts.Here,we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets,and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets(the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function(OTF)).Experiments on reconstructing raw datasets including nonbiological,biological,and simulated samples demonstrate that our method has SR capability,high reconstruction speed,and high robustness to aberration and noise.展开更多
基金The study was funded by the National Key Technologies R&D Program of China(2018YFC0114800 and 2017YFC0109900)the Natural Science Foundation of China(NSFC)(61405238)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20141206)the Key Technologies R&D Program of Jiangsu Province(BE2018666).
文摘Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured illumination can be obtained by either laser interference or projection of fringe patterns.Here,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM system.The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were studied.With the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil objectives.Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated.Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.
基金Supported by the Natural Science Foundation of Beijing under Grant Nos 2162033 and 7182091the National Natural Science Foundation of China under Grant No 21627813
文摘We present a digital micromirror device（DMD） based superpixel method for focusing light through scattering media by modulating the complex field of incident light. Firstly, we numerically and experimentally investigate focusing light through a scattering sample using the superpixel methods with different target complex fields.Then, single-point and multiple-point focusing experiments are performed using this superpixel-based complex modulation method. In our experiment, up to 71.5% relative enhancement is realized. The use of the DMDbased superpixel method for the control of the complex field of incident light opens an avenue to improve the enhancement of focusing light through scattering media.
基金This work is partially supported by the HKSAR Innovation and Technology Commission(ITC)Innovation and Technology Fund(ITF),ITS/179/16FP,as well as the HKSAR Research Grants Council,General Research Fund,Project No.14202815.
文摘An ultrafast spectrum programmable femtosecond laser may enhance the performance of a wide variety of scientific applications,e.g.,multi-photon imaging.In this paper,we report a digital micromirror device(DMD)-based ultrafast pulse shaper,i.e.,DUPS,for femtosecond laser arbitrary amplitude shaping-the first time a programmable binary device reported to shape the amplitudes of ultrafast pulses spectrum at up to 32 kHz rate over a broad wavelength range.The DUPS is highly effcient,compact,and low cost based on the use of a DMD in combination with a transmission grating.Spatial and temporal dispersion introduced by the DUPS is compensated by a quasi-4-f setup and a grating pair,respectively.Femtosecond pulses with arbitrary spectrum shapes,including rectangular,sawtooth,triangular,double-pulse,and exponential profile,have been demonstrated in our experiments.A feedback operation process is implemented in the DUPS to ensure a robust and repeatable shaping process.The total effciency of the DUPS for amplitude shaping is measured to be 27%.
文摘Laser shaping was introduced to maskless projection soft lithography by using digital micro-mirror device (DMD). The predesigned intensity pattern was imprinted onto the DMD and the input laser beam with a Gaussian or quasi-Gaussian distribution will carry the pattern on DMD to etch the resin. It provides a method of precise control of laser beam shapes and?photon-induced curing behavior of resin. This technology provides an accurate micro-fabrication of microstructures used for micro-systems. As a virtual mask generator and a binary-amplitude spatial light modulator, DMD is equivalent to the masks in the conventional exposure system. As the virtual masks and shaped laser beam can be achieved flexibly, it is a good method of precision soft lithography for 2D/3D microstructures.
基金funded by the National Natural Science Foundation of China(62125504,61827825,and 31901059)Zhejiang Provincial Ten Thousand Plan for Young Top Talents(2020R52001)Open Project Program of Wuhan National Laboratory for Optoelectronics(2021WNLOKF007).
文摘Structured illumination microscopy(SIM)achieves super-resolution(SR)by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction.The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain,it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary,besides,the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts.Here,we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets,and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets(the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function(OTF)).Experiments on reconstructing raw datasets including nonbiological,biological,and simulated samples demonstrate that our method has SR capability,high reconstruction speed,and high robustness to aberration and noise.
