笔者前期对植物乳杆菌DMDL 9010(Lactiplantibacillus plantarum DMDL 9010,LP9010)已进行全基因组测序,其NCBI登录号为CP063986-CP063988。该文通过研究LP9010在不同培养条件和培养基成分下的理化指标变化,结合全基因组序列初步分析LP9...笔者前期对植物乳杆菌DMDL 9010(Lactiplantibacillus plantarum DMDL 9010,LP9010)已进行全基因组测序,其NCBI登录号为CP063986-CP063988。该文通过研究LP9010在不同培养条件和培养基成分下的理化指标变化,结合全基因组序列初步分析LP9010降解亚硝酸盐的特性。结果表明,LP9010具有高效降解亚硝酸盐的能力,其亚硝酸盐降解率随着pH的降低而升高;温度为37℃有利于LP9010降解亚硝酸盐;LP9010在营养充分的条件下降解亚硝酸盐的效果最佳,且氧环境和生长因子对其无显著影响(p>0.05)。相应地,在LP9010全基因组中发现了酸耐受(nhaC、gadA、argG和glnR)、冷热耐受(cfa、cspA、hrcA、htpX、cplC、clpE、clpP、clpX和clpL)、氧耐受(catB、trxB、trxA、gor、msrC、msrA和msrB)以及糖转运(pstI、Crr、NagE、ScrA、MtlA、ManX、ManY、ManZ、GatA、GatB、GatC、FruA和FruB)相关的基因,尤其是发现了亚硝酸盐还原酶基因(lmrB和pgl)。总之,LP9010具有高效降解亚硝酸盐的能力和降解亚硝酸盐相关的基因。此结果为植物乳杆菌降解亚硝酸盐的机理提供了新的思路。展开更多
In order to secure the massive heterogeneous medical data for the complex scenarios and improve the information sharing efficiency in healthcare system,a distributed medical data ledger model(DMDL)is proposed in this ...In order to secure the massive heterogeneous medical data for the complex scenarios and improve the information sharing efficiency in healthcare system,a distributed medical data ledger model(DMDL)is proposed in this paper.This DMDL model has adopted the blockchain technology including the function decoupling,the distributed consensus,smart contract as well as multi-channel communication structure of consortium blockchain.The DMDL model not only has high adaptability,but also meets the requirements of the medical treatment processes which generally involve multientities,highly private information and secure transaction.The steps for processing the medical data are also introduced.Additionally,the methods for the definition and application of the DMDL model are presented for three specific medical scenarios,i.e.,the management of the heterogeneous data,copyright protection for medical data and the secure utilization of sensitive data.The advantage of the proposed DMDL model is demonstrated by comparing with the models which are being currently adopted in healthcare system.展开更多
文摘笔者前期对植物乳杆菌DMDL 9010(Lactiplantibacillus plantarum DMDL 9010,LP9010)已进行全基因组测序,其NCBI登录号为CP063986-CP063988。该文通过研究LP9010在不同培养条件和培养基成分下的理化指标变化,结合全基因组序列初步分析LP9010降解亚硝酸盐的特性。结果表明,LP9010具有高效降解亚硝酸盐的能力,其亚硝酸盐降解率随着pH的降低而升高;温度为37℃有利于LP9010降解亚硝酸盐;LP9010在营养充分的条件下降解亚硝酸盐的效果最佳,且氧环境和生长因子对其无显著影响(p>0.05)。相应地,在LP9010全基因组中发现了酸耐受(nhaC、gadA、argG和glnR)、冷热耐受(cfa、cspA、hrcA、htpX、cplC、clpE、clpP、clpX和clpL)、氧耐受(catB、trxB、trxA、gor、msrC、msrA和msrB)以及糖转运(pstI、Crr、NagE、ScrA、MtlA、ManX、ManY、ManZ、GatA、GatB、GatC、FruA和FruB)相关的基因,尤其是发现了亚硝酸盐还原酶基因(lmrB和pgl)。总之,LP9010具有高效降解亚硝酸盐的能力和降解亚硝酸盐相关的基因。此结果为植物乳杆菌降解亚硝酸盐的机理提供了新的思路。
文摘In order to secure the massive heterogeneous medical data for the complex scenarios and improve the information sharing efficiency in healthcare system,a distributed medical data ledger model(DMDL)is proposed in this paper.This DMDL model has adopted the blockchain technology including the function decoupling,the distributed consensus,smart contract as well as multi-channel communication structure of consortium blockchain.The DMDL model not only has high adaptability,but also meets the requirements of the medical treatment processes which generally involve multientities,highly private information and secure transaction.The steps for processing the medical data are also introduced.Additionally,the methods for the definition and application of the DMDL model are presented for three specific medical scenarios,i.e.,the management of the heterogeneous data,copyright protection for medical data and the secure utilization of sensitive data.The advantage of the proposed DMDL model is demonstrated by comparing with the models which are being currently adopted in healthcare system.