Objective To investigate the multiple iron metabolism-related genes expression, its regulation by iron and the expression correlation among the genes in rat tissues. Methods Two groups （n=30） of Sprague-Dawley femal...Objective To investigate the multiple iron metabolism-related genes expression, its regulation by iron and the expression correlation among the genes in rat tissues. Methods Two groups （n=30） of Sprague-Dawley female weanling rats were fed with a control diet and an iron deficient diet respectively for 4 weeks. All rats were then sacrificed, and blood and tissue samples were collected. The routine blood examination was performed with a veterinary automatic blood cell analyzer. Elemental iron levels in liver, spleen and serum were determined by atomic absorption spectrophotometry. The mRNA expression of genes was detected by real-time fluorescence quantitative PCR. Results After 4 weeks, the hemoglobin （Hb） level and red blood cell （RBC） count were significantly lower in the iron deficient group compared with those in the control group. The iron levels in liver, spleen and serum in the iron deficient group were significantly lower than those in the control group. In reference to small intestine, the relative expression of each iron-related gene varied in the different tissues. Under the iron deficiency, the expression of these genes changed in a tissue-specific manner. The expression of most of the genes significantly correlated in intestine, spleen and lung, but few correlated in liver, heart and kidney. Conclusion Findings from our study provides new regulation by iron and correlation among the mRNA divalent metal transporter 1, ferritin, iron regulation protein, hepcidin, ferroportin 1 and hephaestin in intesti understandings about the relative expression, expressions of transferrin receptors 1 and 2, proteins 1 and 2, hereditary hemochromatosis ne, liver, spleen, kidney, heart, and lung of rat.展开更多
MEMS 技术显示了其在微波领域的巨大应用潜力。利用 RF MEMS 开关制造的 RF MEMS 移相器具有插损小、功耗低、宽带宽、体积小等优点,因此成为研究的热点。本文对国际上 R F M E M S的研究和发展进行了比较全面的综述,并介绍了... MEMS 技术显示了其在微波领域的巨大应用潜力。利用 RF MEMS 开关制造的 RF MEMS 移相器具有插损小、功耗低、宽带宽、体积小等优点,因此成为研究的热点。本文对国际上 R F M E M S的研究和发展进行了比较全面的综述,并介绍了 DMTL 移相器,该移相器在 DC￣30GHz 范围内具有较好的线性度,在20GHz 时相移为0°/11.6° /32.6°/48.5°,反射损失好于-11dB,插损小于 -1.8dB。最后分析了各种移相器的特点和设计、制造的难点,对 MEMS 移相器的发展进行了展望。展开更多
基金supported by the National Natural Science Foundation of China (No.30800909)the Fundamental Research Funds for the Central Universities
文摘Objective To investigate the multiple iron metabolism-related genes expression, its regulation by iron and the expression correlation among the genes in rat tissues. Methods Two groups （n=30） of Sprague-Dawley female weanling rats were fed with a control diet and an iron deficient diet respectively for 4 weeks. All rats were then sacrificed, and blood and tissue samples were collected. The routine blood examination was performed with a veterinary automatic blood cell analyzer. Elemental iron levels in liver, spleen and serum were determined by atomic absorption spectrophotometry. The mRNA expression of genes was detected by real-time fluorescence quantitative PCR. Results After 4 weeks, the hemoglobin （Hb） level and red blood cell （RBC） count were significantly lower in the iron deficient group compared with those in the control group. The iron levels in liver, spleen and serum in the iron deficient group were significantly lower than those in the control group. In reference to small intestine, the relative expression of each iron-related gene varied in the different tissues. Under the iron deficiency, the expression of these genes changed in a tissue-specific manner. The expression of most of the genes significantly correlated in intestine, spleen and lung, but few correlated in liver, heart and kidney. Conclusion Findings from our study provides new regulation by iron and correlation among the mRNA divalent metal transporter 1, ferritin, iron regulation protein, hepcidin, ferroportin 1 and hephaestin in intesti understandings about the relative expression, expressions of transferrin receptors 1 and 2, proteins 1 and 2, hereditary hemochromatosis ne, liver, spleen, kidney, heart, and lung of rat.
文摘 MEMS 技术显示了其在微波领域的巨大应用潜力。利用 RF MEMS 开关制造的 RF MEMS 移相器具有插损小、功耗低、宽带宽、体积小等优点,因此成为研究的热点。本文对国际上 R F M E M S的研究和发展进行了比较全面的综述,并介绍了 DMTL 移相器,该移相器在 DC￣30GHz 范围内具有较好的线性度,在20GHz 时相移为0°/11.6° /32.6°/48.5°,反射损失好于-11dB,插损小于 -1.8dB。最后分析了各种移相器的特点和设计、制造的难点,对 MEMS 移相器的发展进行了展望。
