Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-Ethyl-N’-Nitro-N-Nitrosoguanidine (ENNG) . The mean values and standa...Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-Ethyl-N’-Nitro-N-Nitrosoguanidine (ENNG) . The mean values and standard deviations of nuclear DNA and the lesions in the area of different groups were as follows: normal gastric mucosa 10.03±2.30 (arbitrary unit, AU) and 28.76±5.85 (urn2) , atrophic gastritis 12.04 ±3.34 AU and 28.69±8.02μm^2; mild dysplasia 13.52±3.73 AU and 28.23±8.12μm^2; moderate dysplasia 20.88±4.57 AU and 47.58± 10.74μm^2; severe dysplasia 24.01±4.48 AU and 56.64±12.53μm^2; well-differentiated adenocarcinoma 33.07±9.38 AU and 72.99±15.57μm^2, respectively. The nuclear DNA content and nuclear area showed a significant statistical difference between normal gastric mucosa and gastric carcinoma (P【0.01) . The nuclear area of gastric carcinoma increased with DNA content (r =0.73, P【0.01) , and the distribution patterns of DNA content in the histogram showed that diploidy was decreased and polyploidy was increased in cancer. These findings indicated that DNA ploidy patterns and nuclear area could be a useful index in differentiated carcinoma and precancerous lesions.展开更多
文摘Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-Ethyl-N’-Nitro-N-Nitrosoguanidine (ENNG) . The mean values and standard deviations of nuclear DNA and the lesions in the area of different groups were as follows: normal gastric mucosa 10.03±2.30 (arbitrary unit, AU) and 28.76±5.85 (urn2) , atrophic gastritis 12.04 ±3.34 AU and 28.69±8.02μm^2; mild dysplasia 13.52±3.73 AU and 28.23±8.12μm^2; moderate dysplasia 20.88±4.57 AU and 47.58± 10.74μm^2; severe dysplasia 24.01±4.48 AU and 56.64±12.53μm^2; well-differentiated adenocarcinoma 33.07±9.38 AU and 72.99±15.57μm^2, respectively. The nuclear DNA content and nuclear area showed a significant statistical difference between normal gastric mucosa and gastric carcinoma (P【0.01) . The nuclear area of gastric carcinoma increased with DNA content (r =0.73, P【0.01) , and the distribution patterns of DNA content in the histogram showed that diploidy was decreased and polyploidy was increased in cancer. These findings indicated that DNA ploidy patterns and nuclear area could be a useful index in differentiated carcinoma and precancerous lesions.
