Salmon sperm DNA was used as template to assembly and nucleate CdS nanoparticles. Transmission electron microscopy (TEM) images showed that the CdS nanoparticles formed unique nanostructure which present regular and p...Salmon sperm DNA was used as template to assembly and nucleate CdS nanoparticles. Transmission electron microscopy (TEM) images showed that the CdS nanoparticles formed unique nanostructure which present regular and parallel chains along DNA molecular chain. The width of every chain was about 3 nm. Raman and X ray photoelectron energy spectroscopy (XPS) confirmed that the nucleation sites of CdS nanoparticles were phosphate acid groups of DNA.展开更多
CdS nanoparticles were synthesized by using two complementary 2′ phosphorothioate oligo DNA, oligoG 10 SH and oligoC 10 SH as stabalizers. Because the thiol group was modified at phosphate site between C1 and C2 of 5...CdS nanoparticles were synthesized by using two complementary 2′ phosphorothioate oligo DNA, oligoG 10 SH and oligoC 10 SH as stabalizers. Because the thiol group was modified at phosphate site between C1 and C2 of 5′ end, the spatial exposure of DNA residue at C1 site was preponderant compating with the spatial exposure of the nanoparticles when the DNA renatured. The experimental result showed DNA renaturation ratio is about 50% and CdS nanoparticles were assembled into parallel quadrilateral array as the DNA renaturation progressed.展开更多
Monolayer of octadecylamine（ODA） and salmon sperm DNA or salmon sperm DNA-Cd complex were studied on air-water interface by π-A isotherm. After being co-transferred onto substrates by Langmuir-Blodgett technique, a...Monolayer of octadecylamine（ODA） and salmon sperm DNA or salmon sperm DNA-Cd complex were studied on air-water interface by π-A isotherm. After being co-transferred onto substrates by Langmuir-Blodgett technique, atomic force microscope（AFM） measurements show the DNA molecules are packed into lines in the film, due to the interactions between the ODA and DNA molecules. By exposing the ODA and DNA-Cd complex LB film to H2S, needle-like CdS nanoparticles were formed along the DNA lines as characterized by transmission electron microscope（TEM）. Electron diffraction（ED） image indicates that the so prepared needle-like CdS is a new kind of nanostructured materials.展开更多
We report a novel method for rapid,colorimetric detection of a specific deoxyribonucleic acid(DNA)sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two prim...We report a novel method for rapid,colorimetric detection of a specific deoxyribonucleic acid(DNA)sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers.Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple.This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary,and is a promising tool for rapid disease diagnostics and gene sequencing.展开更多
Biologically,there exist two kinds of syntheses:photosynthesis and ATP-driven biosynthesis.The light harvesting of photosynthesis is known to achieve an efficiency of〜95%by the quantum energy transfer of photons.Howev...Biologically,there exist two kinds of syntheses:photosynthesis and ATP-driven biosynthesis.The light harvesting of photosynthesis is known to achieve an efficiency of〜95%by the quantum energy transfer of photons.However,how the ATP-driven biosynthesis reaches its high efficiency still remains unknown.Deoxynucleotide triphosphates(dNTPs)in polymerase chain reaction(PCR)adopt the identical way of ATP to release their energy,and thus can be employed to explore the ATP energy process.Here,using a gold nanoparticle(AuNP)enhanced PCR(AuNP-PCR),we demonstrate that the energy released by phosphoanhydride-bond(PB)hydrolysis of dNTPs is in form of photons(PB-photons)to drive DNA replication,by modulating their resonance with the average inter-AuNP distance(D).The experimental results show that both the efficiency and yield of PCR periodically oscillate with D increasing,indicating a quantized process,but not simply a thermal one.The PB-photon wavelength is further determined to 8.4 pm.All these results support that the release,transfer and utilization of bioenergy are in the form of photons.Our findings of ATP-energy quantum conversion will open a new avenue to the studies of high-efficiency bioenergy utilization,biochemistry,biological quantum physics,and even brain sciences.展开更多
Assembling and ordering nanomaterials into desirable patterns are considerably significant,since the properties of nanomaterials depend not only on the size and shape,but also on the spatial arrangement among the coll...Assembling and ordering nanomaterials into desirable patterns are considerably significant,since the properties of nanomaterials depend not only on the size and shape,but also on the spatial arrangement among the collective building blocks.In this work,the DNA self-assembly technology of hybridization chain reaction(HCR) provided a convenient method to yield long double-strand DNA(dsDNA) to install gold nanoparticles(AuNPs) into one dimensional assembly along the skeleton of dsDNA.Interestingly,the tunable length of AuNPs assemblies along dsDNA chain could be achieved by adjusting the reaction time of HCR,which is based on the formation of covalent bond between Au and the-SH group of DNA.Compared with weak light scattering of single AuNP,these AuNPs assemblies could be clearly imaged under the dark field microscopy,indicating that the light scattering was greatly improved after assembling.展开更多
A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magne...A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays.展开更多
基金ProjectsupportedbytheNationalNaturalScienceFoundationofChina (No .60 1710 2 2 )andtheExcellentYoungTeachersProgramofMinistryofEducation People’sRepublicofChina .
文摘Salmon sperm DNA was used as template to assembly and nucleate CdS nanoparticles. Transmission electron microscopy (TEM) images showed that the CdS nanoparticles formed unique nanostructure which present regular and parallel chains along DNA molecular chain. The width of every chain was about 3 nm. Raman and X ray photoelectron energy spectroscopy (XPS) confirmed that the nucleation sites of CdS nanoparticles were phosphate acid groups of DNA.
文摘CdS nanoparticles were synthesized by using two complementary 2′ phosphorothioate oligo DNA, oligoG 10 SH and oligoC 10 SH as stabalizers. Because the thiol group was modified at phosphate site between C1 and C2 of 5′ end, the spatial exposure of DNA residue at C1 site was preponderant compating with the spatial exposure of the nanoparticles when the DNA renatured. The experimental result showed DNA renaturation ratio is about 50% and CdS nanoparticles were assembled into parallel quadrilateral array as the DNA renaturation progressed.
文摘Monolayer of octadecylamine（ODA） and salmon sperm DNA or salmon sperm DNA-Cd complex were studied on air-water interface by π-A isotherm. After being co-transferred onto substrates by Langmuir-Blodgett technique, atomic force microscope（AFM） measurements show the DNA molecules are packed into lines in the film, due to the interactions between the ODA and DNA molecules. By exposing the ODA and DNA-Cd complex LB film to H2S, needle-like CdS nanoparticles were formed along the DNA lines as characterized by transmission electron microscope（TEM）. Electron diffraction（ED） image indicates that the so prepared needle-like CdS is a new kind of nanostructured materials.
基金This work was supported by the“Bairen Jihua”program of Chinese Academy of Sciences,the Chinese Academy of Sciences/State Administration of Foreign Experts Affairs(CAS/SAFEA)International Partnership Program for Creative Research Teams and Suzhou Bureau of Science and Technology.
文摘We report a novel method for rapid,colorimetric detection of a specific deoxyribonucleic acid(DNA)sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers.Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple.This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary,and is a promising tool for rapid disease diagnostics and gene sequencing.
基金the National Key Research and Development Program of China(No.2018YFE0205501)the National Natural Science Foundation of China Projects(Nos.51763019,U1832125,31630029)the National Supercomputer Center in Tianjin.
文摘Biologically,there exist two kinds of syntheses:photosynthesis and ATP-driven biosynthesis.The light harvesting of photosynthesis is known to achieve an efficiency of〜95%by the quantum energy transfer of photons.However,how the ATP-driven biosynthesis reaches its high efficiency still remains unknown.Deoxynucleotide triphosphates(dNTPs)in polymerase chain reaction(PCR)adopt the identical way of ATP to release their energy,and thus can be employed to explore the ATP energy process.Here,using a gold nanoparticle(AuNP)enhanced PCR(AuNP-PCR),we demonstrate that the energy released by phosphoanhydride-bond(PB)hydrolysis of dNTPs is in form of photons(PB-photons)to drive DNA replication,by modulating their resonance with the average inter-AuNP distance(D).The experimental results show that both the efficiency and yield of PCR periodically oscillate with D increasing,indicating a quantized process,but not simply a thermal one.The PB-photon wavelength is further determined to 8.4 pm.All these results support that the release,transfer and utilization of bioenergy are in the form of photons.Our findings of ATP-energy quantum conversion will open a new avenue to the studies of high-efficiency bioenergy utilization,biochemistry,biological quantum physics,and even brain sciences.
基金supported by the National Natural Science Foundation of China(21535006,21405123)
文摘Assembling and ordering nanomaterials into desirable patterns are considerably significant,since the properties of nanomaterials depend not only on the size and shape,but also on the spatial arrangement among the collective building blocks.In this work,the DNA self-assembly technology of hybridization chain reaction(HCR) provided a convenient method to yield long double-strand DNA(dsDNA) to install gold nanoparticles(AuNPs) into one dimensional assembly along the skeleton of dsDNA.Interestingly,the tunable length of AuNPs assemblies along dsDNA chain could be achieved by adjusting the reaction time of HCR,which is based on the formation of covalent bond between Au and the-SH group of DNA.Compared with weak light scattering of single AuNP,these AuNPs assemblies could be clearly imaged under the dark field microscopy,indicating that the light scattering was greatly improved after assembling.
基金supported by the National Natural Science Foundation of China (21025522 & 20890021)the National Natural Science Foundation of China for Creative Research Groups (20821063)the National Basic Research Program of China (2007CB936404)
文摘A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays.
