运用一对特异性引物扩增并测定了南海硬骨鱼类40个物种89个样本的线粒体DNA的细胞色素c氧化酶Ⅰ(COⅠ)约660bp的部分序列,即COⅠ条码序列。综合比较了《中国鱼类系统检索》、《海洋鱼类志》、FishBase鱼类形态学分类库、ITIS综合分类学...运用一对特异性引物扩增并测定了南海硬骨鱼类40个物种89个样本的线粒体DNA的细胞色素c氧化酶Ⅰ(COⅠ)约660bp的部分序列,即COⅠ条码序列。综合比较了《中国鱼类系统检索》、《海洋鱼类志》、FishBase鱼类形态学分类库、ITIS综合分类学信息系统和生物条码系统(The Barcode of Life Data System,BOLD)的相应形态学与DNA序列资料,在揭示完善我国鱼类检索系统必要性基础上,探讨了COⅠ条码序列在硬骨鱼类辅助物种鉴别和适用性,并对运用该条码序列库完善我国鱼类检索系统的可能途径进行了初步摸索。结果表明,COⅠ条码序列获取便捷,广泛适用硬骨鱼类物种鉴别,并可用于低级分类阶元的系统进化分析。本研究结果可为分子生物学辅助分类、市场监督、资源有序利用和保护提供参考。展开更多
[Objective] A study on the classification of 24 species of Calyptratae entering Ningbo port using DNA barcoding technique was carried out.[Method] The CO I genes of the 24 species of Calyptratae were first sequenced.B...[Objective] A study on the classification of 24 species of Calyptratae entering Ningbo port using DNA barcoding technique was carried out.[Method] The CO I genes of the 24 species of Calyptratae were first sequenced.Based on the comparison and analysis of the obtained sequences,the phylogenetic tree was constructed using MEGA6.0.[Result] The cluster analysis showed the classification of the 24 species of Calyptratae was consistent with the morphological classification at the family and genus levels.However,the cluster analysis could not fully distinguish the closely-related species.[Discussion] The DNA barcoding technique cannot be singly used for classifying and identifying Calyptratae.It should be combined with morphological classification methods,and can be treated as a beneficial supplement for morphological classification methods.展开更多
Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Sh...Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Shrubovych & Bernard,2012.The important morphological characters of Chinese specimens are described in detail.An updated key to Chinese Verrucoentomon species is provided.In addition,their DNA barcodes are sequenced and analyzed.展开更多
文摘运用一对特异性引物扩增并测定了南海硬骨鱼类40个物种89个样本的线粒体DNA的细胞色素c氧化酶Ⅰ(COⅠ)约660bp的部分序列,即COⅠ条码序列。综合比较了《中国鱼类系统检索》、《海洋鱼类志》、FishBase鱼类形态学分类库、ITIS综合分类学信息系统和生物条码系统(The Barcode of Life Data System,BOLD)的相应形态学与DNA序列资料,在揭示完善我国鱼类检索系统必要性基础上,探讨了COⅠ条码序列在硬骨鱼类辅助物种鉴别和适用性,并对运用该条码序列库完善我国鱼类检索系统的可能途径进行了初步摸索。结果表明,COⅠ条码序列获取便捷,广泛适用硬骨鱼类物种鉴别,并可用于低级分类阶元的系统进化分析。本研究结果可为分子生物学辅助分类、市场监督、资源有序利用和保护提供参考。
文摘[Objective] A study on the classification of 24 species of Calyptratae entering Ningbo port using DNA barcoding technique was carried out.[Method] The CO I genes of the 24 species of Calyptratae were first sequenced.Based on the comparison and analysis of the obtained sequences,the phylogenetic tree was constructed using MEGA6.0.[Result] The cluster analysis showed the classification of the 24 species of Calyptratae was consistent with the morphological classification at the family and genus levels.However,the cluster analysis could not fully distinguish the closely-related species.[Discussion] The DNA barcoding technique cannot be singly used for classifying and identifying Calyptratae.It should be combined with morphological classification methods,and can be treated as a beneficial supplement for morphological classification methods.
基金supported by the National Natural Science Foundation of China(31471958,31272298)the Youth Innovation Promotion Association of the CAS(2013183)the Open Project of Key Laboratory of Insect Developmental and Evolutionary Biology,CAS(2009DP17321409)
文摘Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Shrubovych & Bernard,2012.The important morphological characters of Chinese specimens are described in detail.An updated key to Chinese Verrucoentomon species is provided.In addition,their DNA barcodes are sequenced and analyzed.
