DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the...DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies.展开更多
选取牛、羊线粒体12S rRNA基因的特异性引物,以新鲜牛、羊乳样品提取模板DNA,建立了基于DNA结合染料(SYBR Green I)的实时荧光定量PCR方法,用于羊乳制品中牛乳成分的掺假鉴别检测。结果表明,该方法最低可检出新鲜羊奶中掺入2.5%的牛乳成...选取牛、羊线粒体12S rRNA基因的特异性引物,以新鲜牛、羊乳样品提取模板DNA,建立了基于DNA结合染料(SYBR Green I)的实时荧光定量PCR方法,用于羊乳制品中牛乳成分的掺假鉴别检测。结果表明,该方法最低可检出新鲜羊奶中掺入2.5%的牛乳成分,并应用于11份市售羊乳制品样本中的牛、羊源性成分的鉴别。基于DNA结合染料的定量PCR检测无需电泳分离和设计标记DNA探针,具有快速、灵敏、低成本和高通量等优点,可以作为羊乳及其深加工制品中牛乳源性成分掺入检测的有效手段之一。展开更多
文摘DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies.
文摘选取牛、羊线粒体12S rRNA基因的特异性引物,以新鲜牛、羊乳样品提取模板DNA,建立了基于DNA结合染料(SYBR Green I)的实时荧光定量PCR方法,用于羊乳制品中牛乳成分的掺假鉴别检测。结果表明,该方法最低可检出新鲜羊奶中掺入2.5%的牛乳成分,并应用于11份市售羊乳制品样本中的牛、羊源性成分的鉴别。基于DNA结合染料的定量PCR检测无需电泳分离和设计标记DNA探针,具有快速、灵敏、低成本和高通量等优点,可以作为羊乳及其深加工制品中牛乳源性成分掺入检测的有效手段之一。
