期刊文献+
共找到37篇文章
< 1 2 >
每页显示 20 50 100
环F_4[v]/(v^2+v)上DNA码
1
作者 陈磊 李锦 《大学数学》 2019年第2期1-4,共4页
文章研究了环R=F_4[v]/(v^2+v)上的DNA码.基于环R上长度为n的线性码的代数结构,给出了环R上长度为n的线性码是可逆的DNA码的一个充要条件.同时,给出了环R上长度为n的线性码是可逆补DNA码的一个充要条件.
关键词 dna码 可逆的dna码 可逆补dna码 非链环
下载PDF
一种构造GC常重量DNA码的方法 被引量:3
2
作者 梁静 李红菊 +1 位作者 赵凤 丁健 《电子与信息学报》 EI CSCD 北大核心 2019年第10期2423-2427,共5页
GC重量是DNA码的一个重要参数,如何构造满足GC常重量约束的DNA码是一个有趣的问题。该文通过在DNA码与四元码之间建立一个双射,将构造满足GC常重量约束的DNA码转化为构造GC常重量四元码。通过代数的方法,构造了3类满足GC常重量约束的DN... GC重量是DNA码的一个重要参数,如何构造满足GC常重量约束的DNA码是一个有趣的问题。该文通过在DNA码与四元码之间建立一个双射,将构造满足GC常重量约束的DNA码转化为构造GC常重量四元码。通过代数的方法,构造了3类满足GC常重量约束的DNA码。 展开更多
关键词 四元 dna码 GC重量
下载PDF
一种基于前向纠错码的图像DNA加密存储算法 被引量:1
3
作者 姚翔宇 苏燕青 +2 位作者 昝乡镇 许鹏 刘文斌 《信息安全学报》 CSCD 2023年第6期28-36,共9页
近年来DNA(DeoxyriboNucleic Acid)存储发展迅速,实现数字图像DNA存储和安全传输成为有待解决的问题。因此该文提出了一种面向DNA存储的基于前向纠错码的图像加密算法。首先使用动态约瑟夫遍历算法对图像像素点进行行置换和列置换,以消... 近年来DNA(DeoxyriboNucleic Acid)存储发展迅速,实现数字图像DNA存储和安全传输成为有待解决的问题。因此该文提出了一种面向DNA存储的基于前向纠错码的图像加密算法。首先使用动态约瑟夫遍历算法对图像像素点进行行置换和列置换,以消除明文图像相邻像素之间的相关性。其次,使用图像分解方法将明文图像分解为8个子图,然后再重新组合,实现了对图像像素值的置换,从而进一步消除明文图像的纹理特征和破坏其统计学特征。再次,对图像进行全局扩散,使明文的微小变化以扩散的形式影响密文,以抵抗差分攻击。最后使用可纠错DNA编码表将图像加密编码为DNA序列,合成后进行存储。算法将明文图像加密成DNA序列并存储,这种存储方式与传统存储介质相比更为安全。同时,可纠错DNA码使得密文可以在DNA存储环境中可靠读取。该文使用3张常用图像包括lena_gray、peppers_gray、baboon_gray,测试算法的安全性以及在DNA存储环境下的鲁棒性。仿真结果表明,该方法可以有效抵御多种密码学攻击,并且在DNA存储环境下对碱基错误和序列缺失等问题表现出良好的鲁棒性。 展开更多
关键词 图像加密 约瑟夫遍历 图像分解 可纠错dna码
下载PDF
基于微卫星标记的山西糜子资源DNA二维码创建 被引量:1
4
作者 杨哲 陈凌 +2 位作者 王海岗 王瑞云 乔治军 《山西农业科学》 2022年第4期504-509,共6页
通过鉴定糜子种质资源,构建分子身份证,旨在为其保护遗传多样性提供理论依据,以来源于山西黄土高原生态区的21份糜子资源为材料,利用12个SSR标记(六碱基、五碱基和四碱基核苷酸重复序列分别为1、4、7对)筛选出9个遗传稳定性高、重复性... 通过鉴定糜子种质资源,构建分子身份证,旨在为其保护遗传多样性提供理论依据,以来源于山西黄土高原生态区的21份糜子资源为材料,利用12个SSR标记(六碱基、五碱基和四碱基核苷酸重复序列分别为1、4、7对)筛选出9个遗传稳定性高、重复性好的标记,进行遗传多样性分析,利用相关软件构建材料的DNA二维码。结果表明,21份材料在12个位点的遗传多样性参数的观测等位基因平均为3.0000;有效等位基因介于2.5412~2.9959,平均为2.8412;多态性信息含量介于0.6010~0.7720,平均为0.7076;多样性指数介于0.9958~1.0979,平均为1.0661;观测杂合度介于0.5294~0.8883,平均为0.7058;期望杂合度介于0.6238~0.6842,平均为0.6646;Nei′s期望杂合度介于0.6065~0.6662,平均为0.6469。3个核心标记(RYW5、RYW8、RYW11)与其他标记相比相似系数过高,进行剔除处理;基于9个标记(RYW1、RYW2、RYW3、RYW4、RYW6、RYW7、RYW9、RYW10和RYW12)可以将21份材料全部区分。利用条形码在线软件与在线二维码技术分别构建了21份糜子资源的条形码和二维码。 展开更多
关键词 糜子 微卫星 dna二维 种质资源
下载PDF
Identification of 24 Species of Calyptratae Entering Ningbo Port Using DNA Barcoding Technique 被引量:1
5
作者 吴薇 夏德峰 郑炜 《Agricultural Science & Technology》 CAS 2015年第2期235-241,共7页
[Objective] A study on the classification of 24 species of Calyptratae entering Ningbo port using DNA barcoding technique was carried out.[Method] The CO I genes of the 24 species of Calyptratae were first sequenced.B... [Objective] A study on the classification of 24 species of Calyptratae entering Ningbo port using DNA barcoding technique was carried out.[Method] The CO I genes of the 24 species of Calyptratae were first sequenced.Based on the comparison and analysis of the obtained sequences,the phylogenetic tree was constructed using MEGA6.0.[Result] The cluster analysis showed the classification of the 24 species of Calyptratae was consistent with the morphological classification at the family and genus levels.However,the cluster analysis could not fully distinguish the closely-related species.[Discussion] The DNA barcoding technique cannot be singly used for classifying and identifying Calyptratae.It should be combined with morphological classification methods,and can be treated as a beneficial supplement for morphological classification methods. 展开更多
关键词 FLY dna barcode Entering
下载PDF
Three newly recorded species of Acerentomata(Hexapoda:Protura) from China,with analysis of DNA barcodes 被引量:1
6
作者 卜云 钱昌元 栾云霞 《Entomotaxonomia》 CSCD 2017年第1期1-14,共14页
Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Sh... Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Shrubovych & Bernard,2012.The important morphological characters of Chinese specimens are described in detail.An updated key to Chinese Verrucoentomon species is provided.In addition,their DNA barcodes are sequenced and analyzed. 展开更多
关键词 proturans key dna barcodes maxillary gland taxonomy
下载PDF
Discriminant Classification Model of DNA Sequence 被引量:1
7
作者 王显金 阳军 《Agricultural Science & Technology》 CAS 2011年第6期781-784,共4页
[Objective] The research aimed to construct the discriminant classification model of DNA sequence by combining with the biology knowledge and the mathematical method.[Method] According to the polarity nature of side c... [Objective] The research aimed to construct the discriminant classification model of DNA sequence by combining with the biology knowledge and the mathematical method.[Method] According to the polarity nature of side chain radical in the amino acid,the classification information of amino acid which represented the sequence characteristic from the content and array situation of base was extracted from the different sequences that the amino acid content was different.The four-dimension vector was used to represent.Mahalanobis distance and Fisher discriminant methods were used to classify the given sequence.[Result] In the model,the back substitution rates of sample obtained by two kinds of classification methods were both 100%,and the consistent rate of classification was 90%.[Conclusion] In the model,the calculation method was simple,and the accuracy of classification result was higher.It was superior to the discriminant classification model which was only based on the base content. 展开更多
关键词 dna sequence CODON Discriminant analysis FREQUENCY
下载PDF
PCR Amplification System of DNA Barcoding Genes ITS, ITS2 and rbcL from Xanthium
8
作者 胡伟毅 汪连军 盛志超 《Agricultural Science & Technology》 CAS 2013年第9期1212-1214,共3页
[Objective] This study aimed to provide reference and reduce the workload for screening standard DNA barcoding genes of plants. [Method] Three DNA barcoding genes ITS, ITS2 and rbcL were amplified from seven Xanthium ... [Objective] This study aimed to provide reference and reduce the workload for screening standard DNA barcoding genes of plants. [Method] Three DNA barcoding genes ITS, ITS2 and rbcL were amplified from seven Xanthium species under the same PCR condition: PCR amplification was started with initial denaturation at 95 ℃ for 4 min, followed by 35 cycles of denaturation at 94 ℃ for 30 s, annealing at 52 ℃ for 45 s, and extension at 72 ℃ for 45 s; the amplification was completed by holding the reaction mixture at 72 ℃ for 10 min to allow complete extension of PCR, and the PCR products were stored at 4 ℃. [Result] Three DNA barcoding genes ITS, ITS2 and rbcL were all amplified successfully. [Conclusion] This study indicates that PCR amplification conditions for DNA barcoding genes ITS,ITS2 and rbcL in plants may be consistent. 展开更多
关键词 dna barcoding Xanthium ITS ITS2 RBCL
下载PDF
利用SSR创建中国北方黍稷资源DNA身份证 被引量:2
9
作者 胡玉璐 姜百灵 +4 位作者 陈凌 王海岗 曹晓宁 王瑞云 乔治军 《山西农业科学》 2022年第4期478-485,共8页
利用SSR标记,分析中国北方黍稷种质资源的区别与联系,根据DNA碱基的不同,制作出不同的DNA身份证,即构建字符串DNA分子身份证、条形码DNA分子身份证和二维码DNA分子身份证,为黍稷资源管理和保护提供依据。用14对高基元SSR(六碱基4对、五... 利用SSR标记,分析中国北方黍稷种质资源的区别与联系,根据DNA碱基的不同,制作出不同的DNA身份证,即构建字符串DNA分子身份证、条形码DNA分子身份证和二维码DNA分子身份证,为黍稷资源管理和保护提供依据。用14对高基元SSR(六碱基4对、五碱基10对)引物对8个省共20份黍稷资源进行分子身份证的构建,利用PowerMarker 3.25、PopGen 1.32软件计算遗传多样性参数,利用ID Analysis 4.0进行引物组合优化。结果表明,20份材料在10个位点共检测出等位变异10个;Shannon多样性指数(I)为0.7029(RYW7)~1.0880(RYW2),平均为0.9588;观测杂合度(Ho)为0.5000(RYW7)~0.8235(RYW12),平均为0.6866;有效等位变异(Ne)为1.6623(RYW7)~2.7923(RYW12),平均为2.4553;期望观测杂合度(He)为0.4113(RYW7)~0.6862(RYW2),平均为0.6024;多态性信息含量(PIC)为0.4886(RYW3)~0.7766(RYW8),平均为0.6298;Nei’s基因多样性指数(Nei)为0.3984(RYW7)~0.6598(RYW2),平均为0.5828。 展开更多
关键词 黍稷 SSR dna条形 dna二维 中国北方
下载PDF
Application of DNA Barcode Technology to the Identification of Exotic Mosquitoes at Ningbo Port 被引量:1
10
作者 吴薇 王刚 +2 位作者 徐翮飞 赵彤言 夏才军 《Agricultural Science & Technology》 CAS 2017年第12期2454-2457,共4页
[Objective] The study aimed to solve the problem of morphological identi- fication difficulties and propose solutions for the identification of foreign mosquitoes that are difficult to identify. [Method] Based on the ... [Objective] The study aimed to solve the problem of morphological identi- fication difficulties and propose solutions for the identification of foreign mosquitoes that are difficult to identify. [Method] Based on the sequencing, alignment and anal- ysis of COl gene fragment, DNA barcode technology was used to identify 7 exotic mosquitoes, and the phylogenetic analysis was performed using MEGA6.0 and DNASTAR software. Then the morphological characteristics of the mosquitoes were reviewed. [Result[ These mosquitoes were Armigeres subalbatus, Culex gefidus, Anopheles gambiae, and Culiseta incidens. [Conclusion] DNA barcode technology is a useful supplement to the morphological classification method of mosquitoes. 展开更多
关键词 Mosquito: dna barcode ENTRY
下载PDF
Color image encryption algorithm based on chaotic mapping and DNA encoding 被引量:1
11
作者 SHI Ting GAO Li 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2021年第1期68-73,共6页
A color image encryption method combining deoxyribonucleic(DNA)encoding and hyperchaotic mapping is proposed to solve the problems of simple structure,low complexit and low security of the existing encryption system f... A color image encryption method combining deoxyribonucleic(DNA)encoding and hyperchaotic mapping is proposed to solve the problems of simple structure,low complexit and low security of the existing encryption system for low-dimensional chaotic mapping encoding system and a single DNA encoding system.Firstly,according to the information of the plaintext images,the initial values of all chaotic maps and the random matrices with the same size as the plaintext images are iteratively generated.Then,the generated initial values and random matrices are divided into the sub-blocks with the same size.The DNA encoding mode of each sub-block and the DNA operation rules between the sub-blocks are determined by the dynamic hyperchaotic sequence.Finally,the diffusion operation is adopted to achieve a better encryption effect.The simulation results indicate that the proposed encryption algorithm can resist a variety of attacks due to its high complexity,strong security and large key space. 展开更多
关键词 image encryption chaotic mapping deoxyribonucleic(dna)encoding dna computing
下载PDF
Crane flies(Diptera: Tipuloidea: Tipulidae) from Dayaoshan National Nature Reserve, China and analysis of DNA barcodes, with description of one new species in the genus Indotipula
12
作者 门秋雷 薛国喜 +1 位作者 孙梦溦 孙卓 《Entomotaxonomia》 CSCD 2017年第4期251-264,共14页
This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guang... This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guangxiensis Yang Yang, 1988 and Holorusia basiflava Yang Yang, 1993 and female of Pselliophora xanthopimplina Enderlein, 1921 are redescribed and illustrated with new morphological characters. The females of P. guangxiensis and H. basiflava are described and illustrated for the first time. A key for separating known species of Indotipula Edwards, 1931 from China is provided. DNA barcodes of all species in this study are provided and analyzed. 展开更多
关键词 NEMATOCERA dna barcodes REDESCRIPTION taxonomy key
下载PDF
A new record of blue-spotted seabream Pagrus caeruleostictus from Chinese coastal waters documented from morphology and DNA barcoding 被引量:1
13
作者 陈咏霞 刘静 吴仁协 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第2期500-505,共6页
A new record of Pagrus caeruleostictus(Valenciennes,1830),collected from the Beibu Gulf,South China Sea in April 2013,was documented based on morphology and cytochrome oxidase I subunit(COI) gene barcoding analyses. I... A new record of Pagrus caeruleostictus(Valenciennes,1830),collected from the Beibu Gulf,South China Sea in April 2013,was documented based on morphology and cytochrome oxidase I subunit(COI) gene barcoding analyses. It can be distinguished by a combination of the following characteristics: head scaled to above eyes; cheeks with 5 or 6 rows of scales; lateral line scales 51–52; 5 rows of scales above the lateral line; 4 and 6 cuspidate teeth in front of upper and lower jaws,respectively,followed by 2 rows of blunter teeth posteriorly; gill rakers on first arch 12 to 15; D XI–XII +9–11; A III +8–9; the first two dorsal spines very short,the third to fifth extended,filamentous in the young; the first pelvic ray filamentous; silvery pink with dark blue spots on back and sides; caudal fin pinkish; other fins bluish or pinkish; the blue spots generally disappear in large specimens. The present report of P. caeruleostictus suggests that its distribution in Chinese coastal waters may be attributed to human effort,since this species is unlikely to have expanded naturally from the eastern Atlantic to the South China Sea,with no other records from the Indian or Pacific Oceans. We recommend that a precautionary approach should be adopted for the management of P. caeruleostictus. 展开更多
关键词 SPARIDAE Pagrus caeruleostietus cytochrome oxidase I subunit (COI) Chinese Seas
下载PDF
Which Are More Random:Coding or Noncoding DNA Sequences?
14
作者 WUFang ZHENGWei-Mou 《Communications in Theoretical Physics》 SCIE CAS CSCD 2002年第5期637-640,共4页
Evidence seems to show that coding DNA is more random than noncoding DNA, but other conflictingevidence also exists. Based on the third-base degeneracy of codons, we regard the third position of codons as a 'noisy... Evidence seems to show that coding DNA is more random than noncoding DNA, but other conflictingevidence also exists. Based on the third-base degeneracy of codons, we regard the third position of codons as a 'noisy'position. By deleting one fixed position of non-overlapping triplets in a given sequence, three masked sequences may bededuced from the sequence. We have investigated the block-to-site mutual information functions of coding and noncodingsequences in yeast without and with the masking. Characteristics that distinguish coding from noncoding DNA havebeen found. It is observed that the strong correlations in the coding regions may be blocked by the third base of codons,and the proper masking can extract the correlations. Distribution of dimeric tandem repeats of unmasked sequences isalso compared with that of masked sequences. 展开更多
关键词 dna sequences CORRELATION
下载PDF
DNA barcode assessment of Ceramiales(Rhodophyta) in the intertidal zone of the northwestern Yellow Sea 被引量:1
15
作者 杜国英 吴菲菲 +2 位作者 郭皓 薛红凡 茅云翔 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第3期685-695,共11页
A total of 142 specimens of Ceramiales (Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species, 14 genera, ... A total of 142 specimens of Ceramiales (Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species, 14 genera, and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers, namely, partial large subunit rRNA gene (LSU), universal plastid amplicon (UPA), and partial mitochondrial cytochrome c oxidase subunit I gene (COl). No intraspecific divergence was found in our collection for these markers, except for a 1-3 bp divergence in the COI of Ceramium kondoi, Syrnphyocladia latiuscula, and Neosiphoniajaponica. Because short DNA markers were used, the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in GenBank or BOLD (Barcode of Life Data) Systems. Three specimens were presumed as Heterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification, which was the first record in the investigated area, implying that it might he a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers, Heterosiphonia japonica converged with Dasya spp. and was distant from the other Heterosiphonia spp., implying that H.japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species, but the COI had a higher ability to discriminate between species. 展开更多
关键词 dna barcoding CERAMIALES red algae large subunit rRNA gene (LSU) universal plastid amplicon (UPA) cytochrome c oxidase subunit I gene (COI)
下载PDF
Design of Queue for DNA-based Computer
16
作者 李汪根 丁永生 《Journal of Donghua University(English Edition)》 EI CAS 2006年第2期47-50,共4页
This paper discusses the design of the queue for DNA-based computer on the point view of data structure. The nucleotide encodings for all components of the queue are given out formally. The linear double-stranded DNA ... This paper discusses the design of the queue for DNA-based computer on the point view of data structure. The nucleotide encodings for all components of the queue are given out formally. The linear double-stranded DNA molecules are used as the storage structure of the queue, and the basic bio-operations over the queue are described. Furthermore, the comparison between the queue of the electronic computer and that of DNA-based computer are elucidated. To prove the feasibility of our work, nucleotide encodings for an instance of queue are given out. All the biological technology mentioned in this paper can be practically implemented in the laboratory. Based on this work, other data structures could be developed in DNA-based computer. 展开更多
关键词 data structure QUEUE dna-based computer.
下载PDF
A new record of a flathead fish (Teleostei: Platycephalidae) from China based on morphological characters and DNA barcoding 被引量:9
17
作者 秦岩 宋娜 +4 位作者 邹建伟 张朝晖 程光平 高天翔 张秀梅 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2013年第3期617-624,共8页
A new record of Platycephalus sp.1 (sensu Nakabo, 2002) was documented based on morphological characters and DNA barcoding. We collected 174 specimens of the genus Platycephalus from Chinese coastal waters of Dongyi... A new record of Platycephalus sp.1 (sensu Nakabo, 2002) was documented based on morphological characters and DNA barcoding. We collected 174 specimens of the genus Platycephalus from Chinese coastal waters of Dongying, Qingdao, Zhoushan, and Beihai. Samples were identified as Platycephalus sp. 1 morphologically. The coloration, meristic traits, and morphometric measurements are consistent with previously published records. In brief, it is an orange-brown flathead fish with dark brown spots scattered on head and body, lateral line scales 83 to 99 with one or two spine-bearing anteriormost pored scale, no yellow blotch on the caudal fin. Cytochrome oxidase I subunit (COI) gene fragments were sequenced for phylogenetic analysis. The mean evolutionary distance within the species Platycephalus sp. 1 was 0.1%. Net evolutionary distances between Platycephalus sp. 1 and other species of Platycephalus ranged from 10.8% to 19.7%, which is much greater than the threshold for species delimitation. The COl sequence analysis strongly supports the validity ofPlatyceohalus sp. 1 at genetic level. 展开更多
关键词 Platycephalus sp. 1 Chinese coastal waters new record morphological characters cytochromeoxidase I subunit (COI)
下载PDF
Study on DNA Immunization by Recombinants Encoding Japanese Encephalitis Virus prME and E Proteins 被引量:1
18
作者 冯国和 赵桂珍 +3 位作者 Takegami Tsutomu 窦晓光 乔光彦 周子文 《Journal of Microbiology and Immunology》 2003年第1期85-90,共6页
To study the expression characteristic of Japanese encephalitis virus (JEV) prME and E proteins and the efficacy of DNA immunization by different recombinant plasmids containing JEV prME (2001 bp) and E (1500 bp) gene... To study the expression characteristic of Japanese encephalitis virus (JEV) prME and E proteins and the efficacy of DNA immunization by different recombinant plasmids containing JEV prME (2001 bp) and E (1500 bp) genes, two recombinants (pJME and pJE) containing JEV prME and E genes fused with FLAG were constructed and then transfected into HepG2 and COS-1 cells by liposome fusion. The expression feature of FLAG-prME (about 72 kDa) and FLAG-E (about 54 kDa) proteins in transfected cells were analyzed by Western blot and two antibody systems (anti-FLAG and anti-E). BALB/c mice were immunized with 100 μg of two kinds of recombinants by intramuscular injection, and JEV JaGAr-01 strains (10 5 PFU/100 μl)were given to BALB/c mice by intraperioneal injection 3 wk after twice DNA immunization by a lethal virus challenge. BALB/c mice were observed for 21 days after challenge. 80% plaque reduction neutralization test was performed to titrate neutralization antibody before and after viral challenge. It was found that the expression of proteins associated with pJME and pJE was determined in transfected cells with anti-FLAG and a new protein of 11 kDa was detected in HepG2 and COS-1 cells transfected with pJME. Only E (53 kDa) protein was identified as transfected with pJME using anti-E. Higher level of neutralization antibodies and the efficacy of protective immunity were induced with pJME immunization, and were similar to those induced by inactivated Japanese encephalitis vaccine, but were better than those induced with pJE. It concludes that the expression level from prM to E proteins of JEV is different in vitro, and the in vitro expression efficiency of pJME was better than that of pJE. FLAG-prME protein expressed by pJME could be cleaved by peptidase from host. The efficacy of DNA immunization is correlated to the expression characterization of related proteins expressed in vitro. 展开更多
关键词 Japanese encephalitis virus Recombinant plasmid Protein expression dna immunization
下载PDF
Genome-wide Identification and Analysis of DNA Methyltransferases in Grape 被引量:2
19
作者 Fengshan REN Liying YANG +3 位作者 Ling SU Lei GONG Pengfei WANG Yongmei WANG 《Agricultural Science & Technology》 CAS 2017年第10期1781-1787,1794,共8页
DNA methylation is an important epigenetic regulation mechanism, which is catalyzed by DNA methyltransferases. In this study, eight DNA methyltransferase genes were identified in grape genome to analyze the selective ... DNA methylation is an important epigenetic regulation mechanism, which is catalyzed by DNA methyltransferases. In this study, eight DNA methyltransferase genes were identified in grape genome to analyze the selective pressure, gene expression and codon usage bias. The results showed grape DNA methyltransferase MET subfamily underwent relatively strong purifying selection during evolution, while chromomethylase CMT subfamily underwent positive selection during evolution. Under different abiotic(heat, drought or cold) stresses, the expression level of many grape DNA methyltransferase genes changed significantly. The expression level of these genes might be related with cis-regulatory elements of their promoters. The results of codon usage bias analysis showed that synonymous codon bias existed in grape DNA methyltransferase gene family, which might be affected by mutation pressure. These results laid a solid foundation for in-depth study of DNA methyltransferases in grape. 展开更多
关键词 dna methylation dna methyltransferase GRAPE Selective pressure Codon usage bias
下载PDF
Evaluating the Discriminatory Power of DNA Barcodes in Panicoideae, Poaceae
20
作者 Jeongran Lee Chang-Seok Kim In-Yong Lee 《Journal of Agricultural Science and Technology(B)》 2014年第7期533-544,共12页
DNA barcoding is a powerful technique for species identification with little morphological knowledge, by using short sections of DNA from a specific region of the genome. Two core barcode markers, rbcL and matK, and a... DNA barcoding is a powerful technique for species identification with little morphological knowledge, by using short sections of DNA from a specific region of the genome. Two core barcode markers, rbcL and matK, and a supplementary nuclear ribosomal internal transcribed spacer region were used to examine the effectiveness of the markers for Poaceae barcoding using 133 individuals of 36 taxa across 23 genera of Korean Panicoideae. We also aimed to establish a DNA barcode database for the major weeds of Korean Panicoideae. All three markers revealed a good level of amplification and sequencing success. As a single DNA marker, the ITS region achieved the highest species resolution, followed by matK. Resolving power was increased when nrlTS was incorporated into the core barcode markers. The best resolving power was obtained with a combination of matK + ITS with 89.7%, followed by rbcL + matK + ITS with 89.3%. Thus, rbcL may be not necessary as a DNA barcode for Panicoideae species identification, when considering cost and effectiveness. Instead, a combination of matK + ITS is proposed as the most suitable DNA barcode for the species identification of Panicoideae, Poaceae. We conclude that DNA barcoding using a combination of matK + ITS could be one of powerful techniques for the identification of Poaceae species, The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use. 展开更多
关键词 Plant barcode POACEAE core barcode markers NRITS resolving power.
下载PDF
上一页 1 2 下一页 到第
使用帮助 返回顶部