[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 p...[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.展开更多
应用琼酯糖凝胶电泳、S1酶处理及电镜技术 ,从黄瓜线粒体中分离并鉴定了三种环形 DNA类质粒 ,依其分子由大到小 ,分别称之为 p C1、p C2和 p C3.以类质粒凝胶电泳带的荧光扫描值与 DNA类质粒的分子长度之比作为类质粒拷贝数的参照值 ,...应用琼酯糖凝胶电泳、S1酶处理及电镜技术 ,从黄瓜线粒体中分离并鉴定了三种环形 DNA类质粒 ,依其分子由大到小 ,分别称之为 p C1、p C2和 p C3.以类质粒凝胶电泳带的荧光扫描值与 DNA类质粒的分子长度之比作为类质粒拷贝数的参照值 ,对三种类质粒进行荧光扫描分析 ,结果表明 p C3拷贝数明显高于 p C1和 p C2 ;p C2的拷贝数略高于 p展开更多
基金Supported by Youth Foundation of Education Department in Sichuan Province (07ZB060)Youth Science and Technology Innovation Fund in Sichuan Agricultural University~~
文摘[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.
文摘应用琼酯糖凝胶电泳、S1酶处理及电镜技术 ,从黄瓜线粒体中分离并鉴定了三种环形 DNA类质粒 ,依其分子由大到小 ,分别称之为 p C1、p C2和 p C3.以类质粒凝胶电泳带的荧光扫描值与 DNA类质粒的分子长度之比作为类质粒拷贝数的参照值 ,对三种类质粒进行荧光扫描分析 ,结果表明 p C3拷贝数明显高于 p C1和 p C2 ;p C2的拷贝数略高于 p