A water soluble β-cyclodextrin (β-CD) functionalized [60]fullerene (C60-CD) was synthesized.The C60-CD-induced generation of reactive oxygen species (ROS),and DNA-cleavage ability and cytotoxicity of C60-CD were stu...A water soluble β-cyclodextrin (β-CD) functionalized [60]fullerene (C60-CD) was synthesized.The C60-CD-induced generation of reactive oxygen species (ROS),and DNA-cleavage ability and cytotoxicity of C60-CD were studied upon visible light irradiation,demonstrating that the compound is very promising in the applications of photodynamic therapy.The histological analyses demonstrate that C60-CD has no acute or subacute toxicity to living body.展开更多
Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a ...Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a technical challenge. As a crucial structural component of biofilm, extracellular DNA（eDNA） can facilitate initial bacterial adhesion, subsequent development, and final maturation. Inspired by the mechanistic pathways of natural enzymes（deoxyribonuclease）, here we report a novel antibacterial surface by employing cerium（Ce（Ⅳ）） ion to mimic theDNA-cleavage ability of natural enzymes. In this process, the coordination chemistry of plant polyphenols and metal ions was exploited to create an in situ metal-phenolic film on substrate surfaces. Tannic acid（TA） works as an essential scaffold and Ce（Ⅳ） ion acts as both a cross-linker and a destructor of eDNA. The Ce（Ⅳ）-TA modified surface exhibited highly enhanced bacteria repellency and biofilm inhibition when compared with those of pristine or Fe（Ⅲ）-TA modified samples. Moreover, the easily produced coatings showed high stability under physiological conditions and had nontoxicity to cells for prolonged periods of time. This as-prepared DNA-cleavage surface presents versatile and promising performances to combat biomaterial-associated infections.展开更多
基金This study was supported by the Natural Science Foundation of China (Nos.51033002 and 51273090)and the Natural Science Foundation of Jiangsu Province (No.BK2010303).
文摘A water soluble β-cyclodextrin (β-CD) functionalized [60]fullerene (C60-CD) was synthesized.The C60-CD-induced generation of reactive oxygen species (ROS),and DNA-cleavage ability and cytotoxicity of C60-CD were studied upon visible light irradiation,demonstrating that the compound is very promising in the applications of photodynamic therapy.The histological analyses demonstrate that C60-CD has no acute or subacute toxicity to living body.
基金financially supported by the Research Program Funds of Jilin University (Nos.419080500665 and 451170301076)the Natural Science Foundation of Shandong Province (No.ZR2015EM036)
文摘Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a technical challenge. As a crucial structural component of biofilm, extracellular DNA（eDNA） can facilitate initial bacterial adhesion, subsequent development, and final maturation. Inspired by the mechanistic pathways of natural enzymes（deoxyribonuclease）, here we report a novel antibacterial surface by employing cerium（Ce（Ⅳ）） ion to mimic theDNA-cleavage ability of natural enzymes. In this process, the coordination chemistry of plant polyphenols and metal ions was exploited to create an in situ metal-phenolic film on substrate surfaces. Tannic acid（TA） works as an essential scaffold and Ce（Ⅳ） ion acts as both a cross-linker and a destructor of eDNA. The Ce（Ⅳ）-TA modified surface exhibited highly enhanced bacteria repellency and biofilm inhibition when compared with those of pristine or Fe（Ⅲ）-TA modified samples. Moreover, the easily produced coatings showed high stability under physiological conditions and had nontoxicity to cells for prolonged periods of time. This as-prepared DNA-cleavage surface presents versatile and promising performances to combat biomaterial-associated infections.
