The detection of cytokines plays an important role in clinical diagnosis and immune mechanism research of chicken diseases.In this work,a novel and ultrasensitive chemiluminescent(CL)imaging array immunosensor was pro...The detection of cytokines plays an important role in clinical diagnosis and immune mechanism research of chicken diseases.In this work,a novel and ultrasensitive chemiluminescent(CL)imaging array immunosensor was proposed to detect multiple chicken cytokines based on DNAzyme@CuS nanoparticles(DNAzyme@CuSNPs)dual mimic enzyme signal amplification strategy.DNAzyme@CuSNPs owns excellent peroxidase property,which was modified with second antibody(Ab_(2))to prepare DNAzyme@CusNPs detection probe,and demonstrated high catalysis CL imaging signal due to synergistic catalysis.Chicken interleukin-4(ChIL-4)and chicken interferon-y(ChIFN-y)were used as model analysis samples,the DNAzyme@CusSNPs-based CL imaging immunosensor achieved simultaneous and high-throughput detection of ChIL-4 and ChIFN-y with wide linear range of 10^(-3)-10^(2) ng/mL,and the detection limits are 0.41 pg/mL and 0.36 pg/mL,respectively.The multiplex chicken cytokines CL imaging array immunosensor shows a high sensitivity,wide linear range,excellent specificity and acceptable stability.This research opens dual mimic enzyme signal-amplified strategy to develop sensitive CL imaging immunoassay for chicken diseases detection application.展开更多
基金the financial support from the National Natural Science Foundation of China(Nos.21575125 and 21475116)the Natural Science Foundation of Jiangsu Province(Nos.BK20221370 and BK20191434)+2 种基金Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004)Priority Academic Program Development of Jiangsu Higher Education Institution(PAPD),Project for Science and Technology of Yangzhou(No.YZ2022074)Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX22_3462).
文摘The detection of cytokines plays an important role in clinical diagnosis and immune mechanism research of chicken diseases.In this work,a novel and ultrasensitive chemiluminescent(CL)imaging array immunosensor was proposed to detect multiple chicken cytokines based on DNAzyme@CuS nanoparticles(DNAzyme@CuSNPs)dual mimic enzyme signal amplification strategy.DNAzyme@CuSNPs owns excellent peroxidase property,which was modified with second antibody(Ab_(2))to prepare DNAzyme@CusNPs detection probe,and demonstrated high catalysis CL imaging signal due to synergistic catalysis.Chicken interleukin-4(ChIL-4)and chicken interferon-y(ChIFN-y)were used as model analysis samples,the DNAzyme@CusSNPs-based CL imaging immunosensor achieved simultaneous and high-throughput detection of ChIL-4 and ChIFN-y with wide linear range of 10^(-3)-10^(2) ng/mL,and the detection limits are 0.41 pg/mL and 0.36 pg/mL,respectively.The multiplex chicken cytokines CL imaging array immunosensor shows a high sensitivity,wide linear range,excellent specificity and acceptable stability.This research opens dual mimic enzyme signal-amplified strategy to develop sensitive CL imaging immunoassay for chicken diseases detection application.
