Development and ripening of tomato fruit are precisely controlled by transcriptional regulation, which de- pends on the orchestrated accessibility of regulatory proteins to promoters and other c/s-regulatory DNA eleme...Development and ripening of tomato fruit are precisely controlled by transcriptional regulation, which de- pends on the orchestrated accessibility of regulatory proteins to promoters and other c/s-regulatory DNA elements. This accessibility and its effect on gene expression play a major role in defining the develop- mental process. To understand the regulatory mechanism and functional elements modulating morpholog- ical and anatomical changes during fruit development, we generated genome-wide high-resolution maps of DNase I hypersensitive sites （DHSs） from the fruit tissues of the tomato cultivar ＂Moneymaker＂ at 20 days post anthesis as well as break stage. By exploring variation of DHSs across fruit development stages, we pinpointed the most likely hypersensitive sites related to development-specific genes. By detecting binding motifs on DHSs of these development-specific genes or genes in the ascorbic acid biosynthetic pathway, we revealed the common regulatory elements contributing to coordinating gene transcription of plant ripening and specialized metabolic pathways. Our results contribute to a better understanding of the regulatory dynamics of genes involved in tomato fruit development and ripening.展开更多
THE β-like globin gene cluster in human is very homologous to that in mouse. The locuscontrol region (LCR) which is contained in 20 kb upstream of the E-globin gene consists offour erythrold-specif1c DNaseⅠhypersens...THE β-like globin gene cluster in human is very homologous to that in mouse. The locuscontrol region (LCR) which is contained in 20 kb upstream of the E-globin gene consists offour erythrold-specif1c DNaseⅠhypersensitive sites (HS1-HS4) (flg. 1 ). It has beenknown that LCR is critical for the high-level expression of globin genes; its deletion might re-sult in disorder of globin gene expression, even induce severe anemia. Recently,展开更多
文摘Development and ripening of tomato fruit are precisely controlled by transcriptional regulation, which de- pends on the orchestrated accessibility of regulatory proteins to promoters and other c/s-regulatory DNA elements. This accessibility and its effect on gene expression play a major role in defining the develop- mental process. To understand the regulatory mechanism and functional elements modulating morpholog- ical and anatomical changes during fruit development, we generated genome-wide high-resolution maps of DNase I hypersensitive sites （DHSs） from the fruit tissues of the tomato cultivar ＂Moneymaker＂ at 20 days post anthesis as well as break stage. By exploring variation of DHSs across fruit development stages, we pinpointed the most likely hypersensitive sites related to development-specific genes. By detecting binding motifs on DHSs of these development-specific genes or genes in the ascorbic acid biosynthetic pathway, we revealed the common regulatory elements contributing to coordinating gene transcription of plant ripening and specialized metabolic pathways. Our results contribute to a better understanding of the regulatory dynamics of genes involved in tomato fruit development and ripening.
文摘THE β-like globin gene cluster in human is very homologous to that in mouse. The locuscontrol region (LCR) which is contained in 20 kb upstream of the E-globin gene consists offour erythrold-specif1c DNaseⅠhypersensitive sites (HS1-HS4) (flg. 1 ). It has beenknown that LCR is critical for the high-level expression of globin genes; its deletion might re-sult in disorder of globin gene expression, even induce severe anemia. Recently,
