Dof(DNA binding with one finger)蛋白是一类植物特异性转录因子,通常含有200~400个氨基酸和2个主要结构域。该家族成员的N-末端为高度保守的单锌指Dof结构域,具有与DNA和蛋白质相互作用的双重功能,其C末端的氨基酸序列则较为多变,是...Dof(DNA binding with one finger)蛋白是一类植物特异性转录因子,通常含有200~400个氨基酸和2个主要结构域。该家族成员的N-末端为高度保守的单锌指Dof结构域,具有与DNA和蛋白质相互作用的双重功能,其C末端的氨基酸序列则较为多变,是Dof蛋白重要的特异转录调控结构域。研究表明,Dof蛋白作为转录激活物或阻遏物参与了多方面的植物生长发育过程。随着基因组测序技术的发展,已有大量的Dof基因从植物基因组数据库中鉴定出来。该文对近年来国内外有关Dof基因家族的结构特点、全基因组鉴定、蛋白互作以及生物学功能等方面的研究进展进行综述,以期为Dof转录因子的深入研究提供参考。展开更多
The Dof (DNA-binding with one finger) proteins are a class of plant-specific transcription factors that can trigger several processes involved in plant growth and development, as well as in stress responses. Here, we ...The Dof (DNA-binding with one finger) proteins are a class of plant-specific transcription factors that can trigger several processes involved in plant growth and development, as well as in stress responses. Here, we performed a systematic bioinformatics analysis to characterize all Dof genes in common bean, which included analysis of the genome sequence, conserved protein domains, chromosomal locations, subcellular locations, phylogenetic relationships, gene duplications, and gene expression profiles in different tissues. Bioinformatics analysis revealed 36 putative genes related to PvDof that were classified into seven subfamilies (A, B1, B2, C1, C2, D1, and, D2) by comparative phylogenetic analysis. Based on our genome duplication analysis, a total of 36 genes were found to be distributed on all 11 chromosomes, and they expanded through gene duplication in tandem, suggesting the involvement of segmental duplication events in the evolutionary process. Synteny events and phylogenetic comparisons of the Dof proteins of common bean with those of A. thaliana, O. sativa, and G. max L. led to the identification of several orthologous and paralogous genes, which provided further insight into the diversity of the evolutionary characteristics of genes of this family in other plant species. Expression profiles revealed that most of the PvDof genes were expressed in different tissues, indicating that PvDof genes may be involved in various physiological functions during plant development. The results of this study provide additional information and potential biotechnological resources for further understanding the molecular basis of this gene family and consequently improvement of common bean crops.展开更多
水稻(Oryza sativa L.)OsDOF23是植物特异性转录因子DOF(DNA binding with one finger)蛋白家族的一员,但其具体的生物学功能和调节机制不明。本研究以OsDOF23为研究对象,预测了OsDOF23蛋白的理化性质和结构特性,分析了OsDOF23基因的组...水稻(Oryza sativa L.)OsDOF23是植物特异性转录因子DOF(DNA binding with one finger)蛋白家族的一员,但其具体的生物学功能和调节机制不明。本研究以OsDOF23为研究对象,预测了OsDOF23蛋白的理化性质和结构特性,分析了OsDOF23基因的组织表达图谱,及其在水稻群体中的单核苷酸多态性(single nucleotide polymorphisms,SNP)和插入缺失标记(insertion-deletion,InDel)。此外,成功克隆并在大肠杆菌(Escherichia coli)中表达纯化了OsDOF23蛋白的C末端,并以其为抗原制作了OsDOF23的多克隆抗体,用于后续的免疫沉淀实验。经免疫沉淀和液相色谱串联质谱技术检测分析,从水稻总蛋白中得到与OsDOF23共沉淀的蛋白质组信息。经筛选得到37个可能与DOF23互作的蛋白,对这些蛋白进行功能富集分析发现,从生物过程来说,其主要富集于蛋白质代谢和运输功能方面;从分子功能来说,其主要富集于核酸结合活性和转运活性方面。这些结果表明OsDOF23蛋白可能通过与蛋白质代谢、核酸结合和转运等相关功能蛋白互作发挥其生物学功能。展开更多
Dof(DNA binding with one finger)转录因子是一类植物特有的、进化上高度保守的基因家族,对植物多方面的生理过程均发挥调控作用[1]。根据Dof家族中的一个B06基因的cDNA保守序列设计引物,对8个品种花生的B06基因进行PCR扩增克隆相似基...Dof(DNA binding with one finger)转录因子是一类植物特有的、进化上高度保守的基因家族,对植物多方面的生理过程均发挥调控作用[1]。根据Dof家族中的一个B06基因的cDNA保守序列设计引物,对8个品种花生的B06基因进行PCR扩增克隆相似基因,并测定序列。实验成功克隆出四粒红和073103的完整B06基因和其余6个品种的大部分B06基因,其中073103含有一个内含子,其余7个品种不含内含子。通过比较8个品种间B06基因的序列差异与品种性状间的关系,并通过生物信息学对基因及编码蛋白进行分析,探究B06蛋白可能的功能。结果发现:典型的B06蛋白由176个氨基酸组成,在N-端附近37个氨基酸区域处形成保守的单锌指DOF结构;推测在DOF区域后的59-67氨基酸处有核定位信号序列;蛋白序列中有多个N-糖基化、蛋白激酶C磷酸化、酪蛋白激酶Ⅱ磷酸化、N-十四烷酰基化位点,可以接受不同途径的信号刺激,修饰前后发生定位或活性的变,推测Bo6蛋白可能与植物对外界刺激的反应相关。结合性状分析,推测B06蛋白与花生种子中的脂肪和蛋白质含量高低无明显联系。试验为进一步深入研究B06蛋白的功能奠定了基础。展开更多
文摘Dof(DNA binding with one finger)蛋白是一类植物特异性转录因子,通常含有200~400个氨基酸和2个主要结构域。该家族成员的N-末端为高度保守的单锌指Dof结构域,具有与DNA和蛋白质相互作用的双重功能,其C末端的氨基酸序列则较为多变,是Dof蛋白重要的特异转录调控结构域。研究表明,Dof蛋白作为转录激活物或阻遏物参与了多方面的植物生长发育过程。随着基因组测序技术的发展,已有大量的Dof基因从植物基因组数据库中鉴定出来。该文对近年来国内外有关Dof基因家族的结构特点、全基因组鉴定、蛋白互作以及生物学功能等方面的研究进展进行综述,以期为Dof转录因子的深入研究提供参考。
文摘The Dof (DNA-binding with one finger) proteins are a class of plant-specific transcription factors that can trigger several processes involved in plant growth and development, as well as in stress responses. Here, we performed a systematic bioinformatics analysis to characterize all Dof genes in common bean, which included analysis of the genome sequence, conserved protein domains, chromosomal locations, subcellular locations, phylogenetic relationships, gene duplications, and gene expression profiles in different tissues. Bioinformatics analysis revealed 36 putative genes related to PvDof that were classified into seven subfamilies (A, B1, B2, C1, C2, D1, and, D2) by comparative phylogenetic analysis. Based on our genome duplication analysis, a total of 36 genes were found to be distributed on all 11 chromosomes, and they expanded through gene duplication in tandem, suggesting the involvement of segmental duplication events in the evolutionary process. Synteny events and phylogenetic comparisons of the Dof proteins of common bean with those of A. thaliana, O. sativa, and G. max L. led to the identification of several orthologous and paralogous genes, which provided further insight into the diversity of the evolutionary characteristics of genes of this family in other plant species. Expression profiles revealed that most of the PvDof genes were expressed in different tissues, indicating that PvDof genes may be involved in various physiological functions during plant development. The results of this study provide additional information and potential biotechnological resources for further understanding the molecular basis of this gene family and consequently improvement of common bean crops.
文摘水稻(Oryza sativa L.)OsDOF23是植物特异性转录因子DOF(DNA binding with one finger)蛋白家族的一员,但其具体的生物学功能和调节机制不明。本研究以OsDOF23为研究对象,预测了OsDOF23蛋白的理化性质和结构特性,分析了OsDOF23基因的组织表达图谱,及其在水稻群体中的单核苷酸多态性(single nucleotide polymorphisms,SNP)和插入缺失标记(insertion-deletion,InDel)。此外,成功克隆并在大肠杆菌(Escherichia coli)中表达纯化了OsDOF23蛋白的C末端,并以其为抗原制作了OsDOF23的多克隆抗体,用于后续的免疫沉淀实验。经免疫沉淀和液相色谱串联质谱技术检测分析,从水稻总蛋白中得到与OsDOF23共沉淀的蛋白质组信息。经筛选得到37个可能与DOF23互作的蛋白,对这些蛋白进行功能富集分析发现,从生物过程来说,其主要富集于蛋白质代谢和运输功能方面;从分子功能来说,其主要富集于核酸结合活性和转运活性方面。这些结果表明OsDOF23蛋白可能通过与蛋白质代谢、核酸结合和转运等相关功能蛋白互作发挥其生物学功能。
文摘Dof(DNA binding with one finger)转录因子是一类植物特有的、进化上高度保守的基因家族,对植物多方面的生理过程均发挥调控作用[1]。根据Dof家族中的一个B06基因的cDNA保守序列设计引物,对8个品种花生的B06基因进行PCR扩增克隆相似基因,并测定序列。实验成功克隆出四粒红和073103的完整B06基因和其余6个品种的大部分B06基因,其中073103含有一个内含子,其余7个品种不含内含子。通过比较8个品种间B06基因的序列差异与品种性状间的关系,并通过生物信息学对基因及编码蛋白进行分析,探究B06蛋白可能的功能。结果发现:典型的B06蛋白由176个氨基酸组成,在N-端附近37个氨基酸区域处形成保守的单锌指DOF结构;推测在DOF区域后的59-67氨基酸处有核定位信号序列;蛋白序列中有多个N-糖基化、蛋白激酶C磷酸化、酪蛋白激酶Ⅱ磷酸化、N-十四烷酰基化位点,可以接受不同途径的信号刺激,修饰前后发生定位或活性的变,推测Bo6蛋白可能与植物对外界刺激的反应相关。结合性状分析,推测B06蛋白与花生种子中的脂肪和蛋白质含量高低无明显联系。试验为进一步深入研究B06蛋白的功能奠定了基础。