DEVELOPMENT OF DOUBLE-BARRELED CALCIUM ION-SELECTIVE MICROELECTRODE

A new PVC matrix membrane double-barreled calcium ion-selective microelectrode based on liquid ion exchanger has been designed and constructed.The tip diameter of about 2.5μm as well as lower selectivity coefficients for K+,Na and Mg2+are adequate for in-tracellular measurements of Ca activities.The inner wall of the selective channel was made to be hydrophobic by treatment withalkyl-alcohols.By means of this microelectrode some physiologicalphenomena related to Ca2+activities have been studied,and Caconcentrations in clinical microsamples have also been determined.

Applications of the double-barreled data in whole-genome shotgun sequence assembly and analysis

Double-barreled (DB) data have been widely used for the assembly of large ge- nomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly pro- cedure and report on novel applications during the following data-mining processes such as ac- quiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.

Comparison of outcomes following three surgical techniques for patients with severe jejunoileal atresia

Background:Severe jejunoileal atresia is associated with prolonged parenteral nutrition,higher mortality and secondary surgery.However,the ideal surgical management of this condition remains controversial.This study aimed to compare the outcomes of patients with severe jejunoileal atresia treated by three different procedures.Methods:From January 2007 to December 2016,105 neonates with severe jejunoileal atresia were retrospectively reviewed.Of these,42 patients(40.0%)underwent the Bishop–Koop procedure(BK group),49(46.7%)underwent primary anastomosis(PA group)and 14(13.3%)underwent Mikulicz double-barreled ileostomy(DB group).Demographics,treatment and outcomes including mortality,morbidity and nutrition status were reviewed and were compared among the three groups.Results:The total mortality rate was 6.7%,showing no statistical difference among the three groups(P=0.164).The BK group had the lowest post-operative complication rate(33.3%vs 65.3%for the PA group and 71.4%for the DB group,P=0.003)and re-operation rate(4.8%vs 38.8%for the PA group and 14.3%for the DB group,P<0.001).Compared with the BK group,the PA group showed a positive correlation with the complication rate and re-operation rate,with an odds ratio of 4.15[95%confidence interval(CI):1.57,10.96]and 12.78(95%CI:2.58,63.29),respectively.The DB group showed a positive correlation with the complication rate when compared with the BK group,with an odds ratio of 7.73(95%CI:1.67,35.72).The weight-for-age Z-score at stoma closure was–1.22(95%CI:–1.91,–0.54)in the BK group and–2.84(95%CI:–4.28,–1.40)in the DB group(P=0.039).Conclusions:The Bishop–Koop procedure for severe jejunoileal atresia had a low complication rate and re-operation rate,and the nutrition status at stoma closure was superior to double-barreled enterostomy.The Bishop–Koop procedure seems to be an appropriate choice for severe jejunoileal atresia.