The heterocyclic compound 2-amino-N-(2-chloro-6-methylphenyl)thiazole-5-carboxamide(1),designed using 2-chloro-6-methylaniline(2)as the start material,was successfully obtained via multiple synthesis route and finally...The heterocyclic compound 2-amino-N-(2-chloro-6-methylphenyl)thiazole-5-carboxamide(1),designed using 2-chloro-6-methylaniline(2)as the start material,was successfully obtained via multiple synthesis route and finally characterized by IR,1H NMR,and single-cystal X-ray crystallography.To select the suitable candidates for tissue regeneration,the induction activity of the compound on the dental pulp mesenchymal stem cells(DP-MSCs)was evaluated.Firstly,the DP-MSCs cells were isolated and the cell colon formation ability was measured after compound treatment with cell colony determination.After that,the RT-PCT assay was conducted and the relative expression level of the genes related with cell osteogenesis and proliferation was further detected.展开更多
文摘The heterocyclic compound 2-amino-N-(2-chloro-6-methylphenyl)thiazole-5-carboxamide(1),designed using 2-chloro-6-methylaniline(2)as the start material,was successfully obtained via multiple synthesis route and finally characterized by IR,1H NMR,and single-cystal X-ray crystallography.To select the suitable candidates for tissue regeneration,the induction activity of the compound on the dental pulp mesenchymal stem cells(DP-MSCs)was evaluated.Firstly,the DP-MSCs cells were isolated and the cell colon formation ability was measured after compound treatment with cell colony determination.After that,the RT-PCT assay was conducted and the relative expression level of the genes related with cell osteogenesis and proliferation was further detected.
