[Objectives]To study the antioxidant activity of different solvent extracts of Gynura segetum in vitro,so as to provide experimental basis for further study and utilization of medicinal resources of G.segetum.[Methods...[Objectives]To study the antioxidant activity of different solvent extracts of Gynura segetum in vitro,so as to provide experimental basis for further study and utilization of medicinal resources of G.segetum.[Methods]The antioxidant activity of different solvent extracts of G.segetum was determined by UV-vis spectrophotometer using DPPH free radical scavenging method and ABTS^+free radical scavenging method.[Results]The scavenging rates of DPPH and ABTS^+by acetone extract were high,reaching 98.9%and 98.3%,respectively;the scavenging rate of DPPH and ABTS^+by water extract was the lowest,and the scavenging rates were 76.7%and 77.5%,respectively.The order of ABTS^+radical scavenging rate of samples extracted with different extractants was as follows:S 5(acetone extract)>S 1(ethyl acetate extract)>S 6(ethanol extract)>S 4(n-butanol extract)>S 3(methanol extract)>S 2(distilled water extract);the order of DPPH radical scavenging rate of samples extracted with different extractants was as follows:S 5(acetone extract)>S 1(ethyl acetate extract)>S 4(n-butanol extract)>S 6(ethanol extract)>S 3(methanol extract)>S 2(distilled water extract).[Conclusions]The extracts of acetone,ethyl acetate,n-butanol,anhydrous ethanol,methanol and distilled water from the roots of G.segetum had obvious scavenging effects on DPPH and ABTS^+,all had antioxidant components,and acetone had the highest antioxidant effect as an extractant.展开更多
[Objectives] To study in vitro antioxidant activity of extracts from Eleutherine plicata Herb by different extraction methods.[Methods]1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical scavenging method,2,2'-Azino...[Objectives] To study in vitro antioxidant activity of extracts from Eleutherine plicata Herb by different extraction methods.[Methods]1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical scavenging method,2,2'-Azinobis( 3-ehtylbenzothiazolin-6-sulfnic acid) diammonium salt( ABTS) free radical scavenging method,and iron ion reduction ability determination method were applied to measure the extracts of Eleutherine plicata Herb by ethanol extraction,water extraction,and ethanol extraction after water extraction,and comparison was made with positive control Vc( vitamin C). [Results]The ethanol extract,water extract,and ethanol extract after water extraction of Eleutherine plicata Herb had a high DPPH free radical,ABTS free radical scavenging activity,and reducing activity. [Conclusions]E. plicata Herb is a natural antioxidant with high potential.展开更多
文摘[Objectives]To study the antioxidant activity of different solvent extracts of Gynura segetum in vitro,so as to provide experimental basis for further study and utilization of medicinal resources of G.segetum.[Methods]The antioxidant activity of different solvent extracts of G.segetum was determined by UV-vis spectrophotometer using DPPH free radical scavenging method and ABTS^+free radical scavenging method.[Results]The scavenging rates of DPPH and ABTS^+by acetone extract were high,reaching 98.9%and 98.3%,respectively;the scavenging rate of DPPH and ABTS^+by water extract was the lowest,and the scavenging rates were 76.7%and 77.5%,respectively.The order of ABTS^+radical scavenging rate of samples extracted with different extractants was as follows:S 5(acetone extract)>S 1(ethyl acetate extract)>S 6(ethanol extract)>S 4(n-butanol extract)>S 3(methanol extract)>S 2(distilled water extract);the order of DPPH radical scavenging rate of samples extracted with different extractants was as follows:S 5(acetone extract)>S 1(ethyl acetate extract)>S 4(n-butanol extract)>S 6(ethanol extract)>S 3(methanol extract)>S 2(distilled water extract).[Conclusions]The extracts of acetone,ethyl acetate,n-butanol,anhydrous ethanol,methanol and distilled water from the roots of G.segetum had obvious scavenging effects on DPPH and ABTS^+,all had antioxidant components,and acetone had the highest antioxidant effect as an extractant.
基金Supported by Science and Technology Project of Sichuan Province(2015-JY0226)
文摘[Objectives] To study in vitro antioxidant activity of extracts from Eleutherine plicata Herb by different extraction methods.[Methods]1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical scavenging method,2,2'-Azinobis( 3-ehtylbenzothiazolin-6-sulfnic acid) diammonium salt( ABTS) free radical scavenging method,and iron ion reduction ability determination method were applied to measure the extracts of Eleutherine plicata Herb by ethanol extraction,water extraction,and ethanol extraction after water extraction,and comparison was made with positive control Vc( vitamin C). [Results]The ethanol extract,water extract,and ethanol extract after water extraction of Eleutherine plicata Herb had a high DPPH free radical,ABTS free radical scavenging activity,and reducing activity. [Conclusions]E. plicata Herb is a natural antioxidant with high potential.
