分光光度法测定DTPA-HSA冻干品中Sn(Ⅱ)含量

建立了测定ＤＴＰＡ－ＨＳＡ冻干品中亚锡含量的分光光度法。对测定条件进行了选择，最大吸收波长为４６０ｎｍ，摩尔吸光系数为３．２×１０３ｍ２／ｍｏｌ；方法的回收率为１０５．９％±２．２％，相对标准偏差＜５％；Ｓｎ（Ⅱ）的浓度在０～２．４ｍｇ／Ｌ内符合比尔定律。

放射性核素标记DIPA-HSA的研究

介绍了用DTPA(二乙三胺五醋酸)作为双官能团螯合剂与HSA(人血白蛋白)进行偶联,以提高放射性核素标记的HSA的体内稳定性的方法。合成了DTPA酸酐,并将其以固态形式加入HSA水溶液,再经Sephadex G50凝胶柱纯化,得到DTPA-HSA。用^(114)In^m标记的方法,测定了在不同反应介质中DTPA与HSA偶联反应的偶联率。^(114)In^m由^(113)In堆照生产。在邻苯二甲酸盐(pH5)、磷酸盐、Hepes等几种缓冲体系中制备了DTPA-HSA的^(99)Tc^m标记物。小鼠体内分布实验表明,以邻苯二甲酸盐为缓冲介质制备的^(99)Tc^m标记物体内稳定性最好。当HSA浓度为15mg/ml,DTPA与HSA摩尔比(n_(DTPA)/n_(HSA))大于3,Sn^(21)-DTPA摩尔比小于0.5时,标记比较合适。

Lymphoscintigraphy Using Dynamic Imaging and SPECT/CT in Chylothorax

Lymphoscintigraphy readily provides confirmation of chylothorax but not detailed localization of the leakage site. A 77-year-old woman developed traumatic chylothorax and underwent lymphoscintigraphy with radiolabeled albumin to identify the site of lymph leakage. Dynamic imaging demonstrated appearance of focal activity presumably in the upper mediastinum, followed by spread to the left hemithorax. Subsequently, SPECT/CT showed that the leakage site was located in the left side of the upper mediastinum, and this location was confirmed during video-assisted thoracoscopic surgery. Dynamic imaging demonstrated the site of first appearance of abnormal activity, and SPECT/CT enabled detailed localization of the abnormal activity with anatomic correlation. The combination of dynamic imaging with SPECT/CT appears to be recommendable for lymphoscintigraphic assessment of chylothorax.

^（99）Tc^m－DTPA－HSA注射液的放射化学纯度分析

建立了９９Ｔｃｍ－ＤＴＰＡ－ＨＳＡ注射液的放射化学纯度分析方法。确定了分别以８５％甲醇、Ｖ（水）：Ｖ（９５％乙醇）：Ｖ（氢氧化铵）＝５：２：１为溶剂，ＷｈａｔｍａｎＮｏ．１纸为载体的双系统纸层析法。该方法简单、快速。

新的心血池显像剂^（99）Tc^m－DTPA－HSA的实验研究

￣（９９）Ｔｃ￣ｍ－ＤＴＰＡ－ＨＳＡ心血池显像剂于２０只家兔进行实验研究，并与￣（９９）Ｔｃ￣ｍ－ＲＢＣ（体内法）及￣（９９）Ｔｃ￣ｍ－ＨＳＡ进行比较。由￣（９９）Ｔｃ￣ｍ－ＤＴＰＡ－ＨｓＡ所得心血地显像的图像清晰。生物学分布研究表明，￣（９９）Ｔｃ￣ｍ－ＤＴＰＡ－ＨＳＡ在肝和肺的地聚较￣（９９）Ｔｃ￣ｍ－ＲＢＣ及￣（９９）Ｔｃ￣ｍ－ＨＳＡ略低。心／肝放射性比值，￣（９９）Ｔｃ￣ｍ－ＤＴＰＡ－ＨＳＡ为０．８５，￣（９９）Ｔｃ￣ｍ－ＲＢＣ为０．９５，￣（９９）Ｔｃ￣ｍ－ＨＳＡ为０．６２。动物研究结果证明，ＤＴＰＡ与ＨＳＡ螯合后制成的心血池显像剂稳定性好，所得心血池显像清晰，且仅一次注射，值得进一步研究。