Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage

Inflammation plays an important role in nerve defects caused by intracerebral hemorrhage. Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase （Mincle/Syk） signaling pathway is a potential new target for treating cerebral hemorrhage. In this study, we aimed to determine whether acupuncture through Baihui （DU20） to Qubin （GBT） is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway. An intracerebral hemorrhage rat model was established by autol- ogous blood infusion into the caudate nucleus. Acupuncture through Baihui to Qubin was performed for 30 minutes, once every 12 hours, for a total of three times. Piceatannol （34.62 mg/kg）, a Syk inhibitor, was intraperitoneally injected as a control. Modified neurological severity score was used to assess neurological function. Brain water content was measured. Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Minde, Syk, and CARD9. Real-time polymerase chain reaction was used to determine interleukin-1[~ mRNA levels. Hematoxylin-eosin staining was performed to observe histopathological changes. Our re- suits showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARDg, and interkeukin-1β Moreover, this effect was similar to piceatannol. These find- ings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway.

Electroacupuncture at Baihui (DU20) acupoint upregulates mRNA expression of NeuroD molecules in the brains of newborn rats suffering in utero fetal distress

Neuro D plays a key regulatory effect on differentiation of neural stem cells into mature neurons in the brain.Thus,we assumed that electroacupuncture at Baihui（DU20） acupoint in newborn rats exposed to in utero fetal distress would influence expression of Neuro D.Electroacupuncture at Baihui was performed for 20 minutes on 3-day-old（Day 3） newborn Sprague-Dawley rats exposed to in utero fetal distress;electroacupuncture parameters consisted of sparse and dense waves at a frequency of 2–10 Hz.Real-time fluorescent quantitative PCR results demonstrated that m RNA expression of Neuro D,a molecule that indicates Neuro D,increased with prolonged time in brains of newborn rats,and peaked on Day 22.The level of m RNA expression was similar between Day 16 and Day 35.These findings suggest that electro acupuncture at Baihui acupoint could effectively increase m RNA expression of molecules involved in Neuro D in the brains of newborn rats exposed to in utero fetal distress.

Electroacupuncture reduces injury to the blood-brain barrier following cerebral ischemia/reperfusion injury

This study used electroacupuncture at Renzhong （DU26） and Baihui （DU20） in a rat model of cerebral ischemia/reperfusion injury. Neurological deficit scores, western blotting, and reverse transcription-PCR results demonstrated that electroacupuncture markedly reduced neurological deficits, decreased corpus striatum aquaporin-4 protein and mRNA expression, and relieved damage to the blood-brain barrier in a rat model of cerebral ischemia/reperfusion injury. These results suggest that electroacupuncture most likely protects the blood-brain barrier by regulating aquaporin-4 expression following cerebral ischemia/reperfusion injury.

百会穴、大椎穴在血管性痴呆治疗中的作用机制探讨

血管性痴呆(VD)是世界第二大痴呆疾病,也是唯一可以防治的痴呆类型。近年来随着人口老龄化与脑卒中发病率的升高,VD的发生率也呈逐年上升之势。针灸作为一种传统的治疗方法,在治疗缺血性脑病方面具有较为肯定的临床疗效。百会和大椎作为各类针灸疗法治疗VD的核心取穴,作用关键,在临床中运用极广,然而现代研究尤其是其作用机理的探讨尚显匮乏,鲜见报道。文章拟从中医理论依据、现代研究及作用机制方面,对百会穴、大椎穴在血管性痴呆的治疗作用作一探讨,以期为临床治疗提供理论参考。

电针肾俞、膈俞、百会穴对拟血管性痴呆小鼠脑缺血、缺氧、脑水肿的影响

目的 :观察电针肾俞、膈俞、百会穴对拟血管性痴呆小鼠脑缺血、缺氧、脑水肿的影响。方法 :复制脑缺血再灌注拟血管性痴呆小鼠模型 ,电针肾俞、膈俞、百会穴 ,分别于术后 7d、1 5d、30 d,与喜德镇对照治疗 ,记录小鼠张口喘气时间 ,计算脑指数及脑含水量。结果 :造模导致了小鼠脑缺血、缺氧 ,早期还出现脑组织水肿 ,表现为张口喘气时间缩短 ,脑指数和脑含水量增加。而电针和喜德镇均可延长张口喘气时间、降低脑指数和脑含量 ,具有抗脑缺氧和减轻脑水肿程度的作用 ,但疗效以电针为优。结论 :电针肾俞、膈俞、百会穴对该模型出现的脑缺血、缺氧状态 ,及早期的脑组织水肿有显著改善作用 ,此可能为针刺治疗 VD作用机理之一。

论百会穴穴性及临床应用

百会穴是督脉的重要穴位,督脉由此入络脑腑,故可醒脑神、补脑髓;该穴更是督脉与诸经交会之所,有升阳举陷的治疗作用。历代医家对此穴有精辟的理论阐述及丰富的临床使用经验,现代医疗临床上更是根据其经脉络属关系逐步扩大其临床应用,在经典中医理论的指导下辨证选穴,不断总结实践经验,并上升为理论观点以指导临床。除此之外,现代医学对百会穴亦有一定的研究成果,对其生理反应和病理作用也有一定的阐述,根据西医学的对症原则应用百会穴一样取得了较好的临床效果。由此可见百会穴是临床上的常用穴、特效穴,几千年来一直广泛应用于临床,并取得明显的治疗效果,该文主要将近年来百会穴的临床应用情况以及程为平教授对百会穴的临床使用经验整理并进行初步探讨,以便更好的指导临床。

针刺四神聪、百会治疗失眠68例

目的:观察针刺四神聪、百会治疗失眠的临床疗效。方法:选取2014年12月—2016年10月在河南中医药大学第三附属医院治疗的失眠患者133例,随机分为治疗组68例和对照组65例。治疗组给予针刺四神聪、百会为主治疗,对照组给予口服艾司唑仑片治疗。比较两组治疗前后匹兹堡睡眠质量指数量表(PSQI)评分,观察两组临床疗效。结果:治疗组治疗后PSQI评分优于对照组,差异有统计学意义(P<0.05);治疗组有效率为91.17%,对照组有效率为78.46%,两组比较差异有统计学意义(P<0.05)。结论:以四神聪、百会为主针刺治疗失眠较艾司唑仑片有更好的改善睡眠效果。

百会穴久留针治疗椎动脉型颈椎病疗效观察

目的:观察百会穴久留针治疗椎动脉型颈椎病的疗效。方法:将120例椎动脉型颈椎病患者按就诊顺序随机分为治疗组和对照组各60例。治疗组采用百会穴久留针8h和局部穴位电针疗法,对照组采用百会穴一般留针时间30m in和局部穴位电针疗法。结果:治疗组治愈率为70.0%,总有效率为98.3%;对照组治愈率为45.0%,总有效率为86.7%。两组比较,治愈率和总有效率差异均有显著意义(P<0.01,P<0.05)。结论:百会穴久留针治疗椎动脉型颈椎病有较好疗效。

Anti-aging effects of moxa cone moxibustion As a free radical scavenger complement

Therapies that complement free radical scavenging are an important approach for treating aging in the brain. In the present study, two formulations of moxa cone moxibustion were applied at acupoints Zusanfi （ST 36） and Xuanzhong （GB 39）, and at acupoints Baihui （DU 20） and Guanyuan （RN 4）, in D-galactose-induced senile mice. The results revealed that moxa cone moxibustion improved total superoxide dismutase and Cu/Zn-superoxide dismutase activity in the homogenates of the cerebral tissue, as well as ameliorating deficits in neuronal morphology and neuronal density in the cerebral cortex and hippocampal CA3. Moxa cone moxibustion also enhanced learning and memory functions of senile mice. Moxa cone moxibustion at Zusanli, Xuanzhong, Baihuiand Guanyuan acupoints can thus be used to complement free radical scavengers, with efficacy that is equal to that of electroacupuncture at Zusanliand Xuanzhong, and superior to that of nimodipine treatment.

同伴支持小组协同护理模式对肠造口患者自我护理和适应水平的影响

目的探讨同伴支持小组协同护理管理模式对永久肠造口患者早期自我护理和适应水平的影响。方法将116例首次行永久肠造口手术出院后的患者随机分为对照组60例和观察组56例,对照组实施常规延续性护理,观察组在此基础上增加同伴支持小组协同护理。出院时、6个月后应用自我护理能力测定量表和中文版造口患者社会心理适应量表进行效果评价。结果干预后两组患者自我护理水平和造口社会适应水平较干预前显著提高(均P<0.05),其中观察组干预效果显著优于对照组(均P<0.05)。结论同伴支持小组协同护理管理模式能够较好地提高首次行永久肠造口患者早期自我护理能力和社会生活适应水平。

针刺百会穴对睡眠剥夺大鼠模型脑电图α波、β波频率的影响

目的:通过睡眠剥夺大鼠模型脑电图α波、β波的频率,分析针刺百会穴对失眠的治疗效果。方法:采用水平台法建立睡眠剥夺大鼠模型,分为实验组、假穴位组和模型组,以正常喂养的大鼠作为空白对照组。实验组行百会穴针刺治疗,假穴位组行百会穴左侧旁0.2cm的假穴位针刺治疗,模型组、空白组不予以治疗。在72h睡眠剥夺结束后记录各组大鼠脑电波数据,观察针刺百会穴对睡眠剥夺大鼠模型脑电图α波、β波频率的影响。结果:针刺前,实验组、假穴位组、模型组大鼠α波、β波频率无统计学差异(P>0.05),且α波均低于空白组,β波均高于空白组,说明睡眠剥夺可影响正常脑电波频率,提升大鼠大脑皮层兴奋性;针刺假穴位组大鼠脑电图变化与模型组比较无统计学差异(P>0.05),且治疗后大鼠的狂躁表现无显著改善,没有立刻进入睡眠;针刺治疗中,实验组大鼠α波频率上升、β波频率下降,趋向于正常,且治疗后立刻进入睡眠状态,说明针刺百会穴可降低睡眠剥夺模型的大脑皮层兴奋度。结论:针刺百会穴对睡眠剥夺大鼠模型具有安神作用。

基于数据挖掘分析百会、大椎穴的临床应用

目的：通过数据挖掘，总结分析百会、大椎穴配伍在临床应用中的情况。方法：根据中国学术期刊网络出版总库、中国科技期刊数据库，检索1982至2016年采用督脉穴位百会、大椎配伍为主穴治疗的疾病，进行归类总结。结果：百会、大椎多与足三里、肾俞、水沟等胃经、膀胱经、督脉穴位配伍，主治疾病多达33种，涉及多个系统，以电针及普通针刺为主要治疗手段。结论：百会、大椎配伍其他穴位对于神经系统疾病的治疗发挥重要作用。

High-frequency electroacupuncture evidently reinforces hippocampal synaptic transmission in Alzheimer＇s disease rats

The frequency range of electroacupuncture in treatment of Alzheimer＇s disease in rats is commonly 2-5 Hz（low frequency） and 50-100 Hz（high frequency）. We established a rat model of Alzheimer＇s disease by injecting β-amyloid 1-42（Aβ1-42） into the bilateral hippocampal dentate gyrus to verify which frequency may be better suited in treatment. Electroacupuncture at 2 Hz or 50 Hz was used to stimulate Baihui（DU20） and Shenshu（BL23） acupoints. The water maze test and electrophysiological studies demonstrated that spatial memory ability was apparently improved, and the ranges of long-term potentiation and long-term depression were increased in Alzheimer＇s disease rats after electroacupuncture treatment. Moreover, the effects of electroacupuncture at 50 Hz were better than that at 2 Hz. These findings suggest that high-frequency electroacupuncture may enhance hippocampal synaptic transmission and potentially improve memory disorders in Alzheimer＇s disease rats.

Hippocampal gene expression in a rat model of depression after electroacupuncture at the Baihui and Yintang acupoints

Preliminary basic research and clinical findings have demonstrated that electroacupuncture ther- apy exhibits positive effects in ameliorating depression. However, most studies of the underlying mechanism are at the single gene level; there are few reports regarding the mechanism at the whole-genome level. Using a rat genomic gene-chip, we profiled hippocampal gene expression changes in rats after electroacupuncture therapy. Electroacupuncture therapy alleviated depres- sion-related manifestations in the model rats. Using gene-chip analysis, we demonstrated that electroacupuncture at Baihui （DU20） and Yintang （EX-HN3） regulates the expression of 21 genes. Real-time PCR showed that the genes Vgf, lgf2, Trnp32, Loc500373, Hifla, Folrl, Nrnb, and Rtn were upregulated or downregulated in depression and that their expression tended to nor- malize after electroacupuncture therapy. These results indicate that electroacupuncture at Baihui and Yintang modulates depression by regulating the expression of particular genes.

针刺水沟穴、百会穴联合足运感区对脑卒中恢复期患者肢体功能、颅内血流动力学的影响

目的:观察针刺水沟穴、百会穴联合足运感区对脑卒中恢复期患者肢体功能、颅内血流动力学的影响。方法:选取163例脑卒中恢复期患者,按随机数字表法分为足运感区组81例及联合针刺组82例。足运感区组针刺足运感区治疗,联合针刺组给予针刺水沟穴、百会联合足运感区进行治疗。比较2组临床疗效,比较2组治疗前后神经营养素-3(NT-3)、神经生长因子(NGF)水平、中医证候评分、颅内血流动力学[收缩峰血流速度(Vs)、舒张末血流速度(Vd)、平均血流速度(Vm)]指标值、美国国立卫生研究院卒中量表(NIHSS)评分的变化。结果:治疗后,2组NT-3、NGF水平均较治疗前上升,联合针刺组NT-3、NGF水平高于足运感区组(P<0.05)。治疗后,2组中医证候主症评分、总分均较治疗前下降(P<0.05),联合针刺组主症评分、总分均低于足运感区组(P<0.05)。治疗后,2组Vs、Vd、Vm指标值均较治疗前上升(P<0.05),联合针刺组Vs、Vd、Vm指标值高于足运感区组(P<0.05)。治疗后,2组NIHSS上肢评分、NIHSS下肢评分均较治疗前下降,联合针刺组NIHSS上肢评分、NIHSS下肢评分均低于足运感区组(P<0.05)。联合针刺组临床疗效为97.56%,足运感区组为91.12%,2组比较,差异有统计学意义(P<0.05)。结论:针刺水沟穴、百会联合针刺足运感区治疗脑卒中恢复期,能恢复患者上下肢肢体功能,改善颅内血流动力学,临床疗效较好。

Comparative Study of the Specificities of Needling Acupoints DU20, DU26 and HT7 in Intervening Vascular Dementia in Different Areas in the Brain on the Basis of Scale Assessment and Cerebral Functional Imaging

Objective： Using methods of clinical scale assessment and cerebral functional imaging to compare the relative specificity of needling acupoints Baihui （DU20）, Shuigou （DU26） and Shenmen （HTT） in intervening vascular dementia （VD） in different areas in the brain. Methods： Fifty patients with VD were randomized into 5 groups. Needling on conventionally used acupoints of hand and foot three Yang-meridians aiming at hemiplegia was applied to the patients in Group A, and needling on DU20 to Group B, on DU26 to Group C, on HT7 to Group D and on all the three to Group E was applied additionally. Assessments of Mini Mental State Examination （MMSE）, Activities of Daily Living （ADL） and Family Attitude Questionnaire （FAQ） were made. And the positron emission computerized tomography （PET） and single photon emission computerized tomography （SPECT） examinations were conducted in 5 selected patients from each group before and after treatment. Results： Needling on conventional acupoints plus DU20 could effect the inner temporal system, thalamencephalon system and prefrontal cortical system to improve memory and executive capacity of VD patients; conventional acupoints plus DU26 could effect more to the prefrontal cortical system to obviously elevate the executive capacity; that plus HT7 would reveal an effect similar to but rather weaker than plus DU20, and effect more to memory; and that plus all the three simultaneously could effect rather roundly multiple aspects of the nervous system related to intellectual activities, to elevate the recognition and enhance the executive capacity. Conclusion： Needling on various acupoints like DU20, DU26 and HT7 have effects on different brain areas.

Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse

Drugs can cause obvious damage to the brain. To verify the relationship between acupuncture, neurotrophic factor expression and brain cell structural changes, this study established a rat model of heroin relapse using intramuscular injection of increasing amounts of heroin. During the detoxification period, rat models received acupuncture at Baihui （DU20） and Dazhui （DU14）. Electron microscopy demonstrated that the structure of the ventral tegmental area in heroin relapse rats gradually became normalized after acupuncture treatment. Immunohistochem- ical staining exhibited that the expression of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor increased in the ventral tegmental area following acupuncture. Moreover, the effects were similar to that of methadone, a type of medicine called an opioid. Results suggested that acupuncture at Baihui and Dazhui protected brain neurons against injury in rats with heroin relapse by promoting brain-derived neurotrophic factor and glial cell line-de-rived neurotrophic factor expression.

电针结合传统针刺百会、大椎穴对脑缺血模型大鼠GAP-43表达的影响

目的:探讨针刺百会、大椎对脑缺血模型大鼠GAP-43表达的影响,探索其对神经功能再生的影响机制,为临床针刺百会、大椎治疗缺血性脑血管病提供实验依据。方法:选取SPF级雄性大鼠150只,体质量230~270 g,采用随机数字法随机分为五个大组,每组30只大鼠。各组又随机平均分为术后3 d、7 d、21 d 3个不同时段亚组,每组10只。采用线栓法制备脑缺血模型。电针组针刺主穴后,接G6805-2型电针仪,每天1次,每次30 min。针刺组予针刺干预,不接电针,每5 min行针1次,每天1次,电针结合传统针刺组,行电针及传统针刺方法,每天1次。模型组术后不予处理,假手术组使实验大鼠大脑中动脉暴露,不予栓线。各组大鼠在造模后3 d、7 d、21 d 3个不同时段采用神经功能缺损评分及免疫组化法检测脑缺血周围组织GAP-43蛋白表达情况,采用JD801形态学图像分析系统进行分析,对结果进行统计学分析。结果:各时间段模型组神经功能评分与假手术组比较显著升高,差异具有统计学意义(P<0.05);在各时间段其他组与模型组比较,电针组、传统针刺组、电针结合传统针刺组神经功能缺损评分均较模型组降低,差异具有统计学意义(P<0.05);术后第7天和第21天,与电针组、传统针刺组比较,电针结合传统针刺组大鼠神经功能缺损评分降低,差异具有统计学意义(P<0.05)。各时间段模型组脑组织GAP-43蛋白的免疫表达与假手术组比较显著升高,差异具有统计学意义(P<0.05)。在各时间段其他组与模型组比较,电针组、传统针刺组、电针结合传统针刺组大鼠相应区域GAP-43免疫活性较模型组增高,差异具有统计学意义(P<0.05);术后第3天、第7天和第21天,与电针组、传统针刺组比较,电针结合传统针刺大鼠GAP-43免疫活性显著增高,差异具有统计学意义(P<0.05)。结论:电针结合传统针刺百会、大椎能促进各时间点脑缺血大鼠脑组织内GAP-43表达,改善脑血管的缺血状态。

A Comparative Study on the Acupoints of Specialty of Baihui, Shuigou and Shenmen in Treating Vascular Dementia

Objective： To study the relative specialty of the acupoints of Baihui （DU20）, Shuigou （DU26） and Shenmen （HTT） in treating vascular dementia （VD）. Methods： Fifty patients suffering from VD were divided into 5 groups randomly and treated by conventional acupuncture for hemiplegia, or conventional acupuncture plus needling in DU20, DU26, HT7 respectively or simultaneously. Clinical symptoms were observed, and the scales such as Hasegawa＇s dementia scale-recension （HDS-R）, activities of daily living （ADL） and functional activities questionnaire （FAQ） were used for assessment before and after acupuncture treatment. The scores were processed statistically. Results： DU20 and HT7 could improve the abilities of memory, orientation, reaction and reduce adamancy and trance. Bedsides, DU 20 was helpful in improving the abilities of understanding, calculating and social adapting. While DU26 is helpful in treating retardation, failure in daily activities, trance and poor memory. The combination of the 3 acupoints could improve the intelligence and social adapting ability of VD patients. Conclusion： DU20, DU26 and HT7 had its own effect in treating VD, but the effect of three points used in combination was the best.

电针刺激癫痫大鼠“大椎”“百会”后对海马小胶质细胞及神经保护作用的研究

目的:研究电针刺激对癫痫发作后脑内海马小胶质细胞和神经元损伤的影响。方法:制备锂-匹罗卡品癫痫大鼠模型,通过电针刺激大鼠"大椎""百会"2个穴位,采用免疫组化及Nissl染色方法,分别计数各组海马CA1区的OX-42阳性细胞数量、神经元数量及形态变化。结果:电针刺激组中OX-42阳性细胞的数量较单纯癫痫组降低46.7%(P<0.05),同时电针刺激组神经元损伤情况较单纯癫痫组显著改善(P<0.05)。结论:电针刺激癫痫大鼠"大椎""百会"两穴位可抑制小胶质细胞介导的炎性反应,具有神经保护作用。