Analysis and Study on Quality Control of Yao Medicine Damnacanthus macrophyllus Based on Methods in Chinese Pharmacopoeia

[Objectives]To establish a quality analysis method for Yao medicine Damnacanthus macrophyllus Sieb.ex Miq.var.giganteus(Makino)Koidz..[D.indicus Gaertn.f.var.giganteus Makino].[Methods]The moisture,total ash and alcohol extracts of D.macrophyllus medicinal materials were determined according to the experimental methods in the Chinese Pharmacopoeia(2020).[Results]All the 10 batches of D.macrophyllus medicinal materials from different collection times and different regions in Guangxi had the same plant morphological characteristics.The experimental results indicate that the moisture was 9.81%-11.52%,the total ash content was 7.37%-9.53%,the acid-insoluble ash content was 1.10%-1.97%,and the extract was 8.25%-10.22%.[Conclusions]This study provides a scientific basis for establishing a quality standard for Yao medicine D.macrophyllus medicinal materials,and it is expected to better utilize and develop D.macrophyllus.

虎刺中总黄酮的含量测定

研究超声波辅助技术测定虎刺中总黄酮含量的方法。通过线性关系试验、精密度试验、稳定性试验、回收率试验和测定样品中总黄酮的含量,研究了利用超声波辅助技术测定虎刺中总黄酮含量的方法。在线性关系试验中,总黄酮浓度在8~60μg/ml,线性范围良好。在精密度试验中,RSD值为0.53%,表明精密度较好。在稳定性试验中,RSD值为1.12%,表明在0~60 min之间的稳定性较好。在回收率试验中,其平均回收率为99.9%,RSD为0.55%（n=5）。说明该方法具有良好的加样回收率。用该法测得的虎刺中总黄酮的平均含量为1.413%。该方法具有精密度、稳定性均较好,回收率高等优点,可以作为虎刺中总黄酮含量的测定方法。

反相高效液相色谱法测定不同产地虎刺中齐墩果酸和熊果酸的含量

目的:建立反相高效液相色谱法测定不同产地虎刺中齐墩果酸和熊果酸的含量。方法:虎刺样品以95%乙醇溶液超声提取,分析柱选用Kromasil C18色谱柱,以甲醇-2.0%磷酸水溶液(90∶10,v/v)进行洗脱,柱温:25℃,流速:0.8 mL/min,检测波长:210 nm。结果:齐墩果酸在0.124～1.24μg范围内,与峰面积呈良好的线性关系(r=0.9997),平均回收率为97.6%(RSD=2.2%);熊果酸在0.192～1.92μg范围内,与峰面积呈良好的线性关系(r=0.9999),平均回收率为102.4%(RSD=1.9%)。结论:该二种成分均以湖北产虎刺中含量最高,方法操作简便,精密度高,可为虎刺的质量控制和开发利用提供实验依据。

均匀设计优选绣花针多糖的提取工艺

目的优选绣花针中多糖的提取工艺。方法用均匀设计方法设计水提取工艺,以提取物中多糖含量为测定指标,考察提取温度,提取时间,料液比3个因素对有效成分多糖提取的影响。结果最佳工艺为提取温度90℃,提取时间2 h,料液比1:50(g/m l),绣花针多糖的提取率为14.59%。结论提取温度、提取时间及料液比对绣花针多糖的提取率有影响。