Diagnostic and therapeutic applications of water-immersion colonoscopy

Colonoscopy techniques combining or replacing air insufflation with water infusion are becoming increasinglypopular.They were originally designed to reduce colonic spasms,facilitate cecal intubation,and lower patient discomfort and the need for sedation.These maneuvers straighten the rectosigmoid colon and enable the colonoscope to be inserted deeply without causing looping of the colon.Water-immersion colonoscopy minimizes colonic distension and improves visibility by introducing a small amount of water.In addition,since pain during colonoscopy indicates risk of bowel perforation and sedation masks this important warning,this method has the potential to be the favored insertion technique because it promotes patient safety without sedation.Recently,this water-immersion method has not only been used for colonoscope insertion,but has also been applied to therapy for sigmoid volvulus,removal of lesions,lower gastrointestinal bleeding,and therapeutic diagnosis of abnormal bowel morphology and irritable bowel syndrome.Although a larger sample size and prospective head-to-head-designed studies will be needed,this review focuses on the usefulness of waterimmersion colonoscopy for diagnostic and therapeutic applications.

Evidence-Based Dampness-Heat ZHENG(Syndrome) in Cancer: Current Progress toward Establishing Relevant Animal Model with Pancreatic Tumor

Cancer is one of the deadliest diseases affecting the health of human beings. With limited therapeutic options available, complementary and alternative medicine has been widely adopted in cancer management and is increasingly becoming accepted by both patients and healthcare workers alike. Chinese medicine characterized by its unique diagnostic and treatment system is the most widely applied complementary and alternative medicine. It emphasizes symptoms and ZHENG(syndrome)-based treatment combined with contemporary disease diagnosis and further stratifies patients into individualized medicine subgroups. As a representative cancer with the highest degree of malignancy, pancreatic cancer is traditionally classified into the "amassment and accumulation". Emerging perspectives define the core pathogenesis of pancreatic cancer as "dampness-heat" and the respective treatment "clearing heat and resolving dampness" has been demonstrated to prolong survival in pancreatic cancer patients, as has been observed in many other cancers. This clinical advantage encourages an exploration of the essence of dampness-heat ZHENG(DHZ) in cancer and investigation into underlying mechanisms of action of herbal formulations against dampness-heat. However, at present, there is a lack of understanding of the molecular characteristics of DHZ in cancer and no standardized and widely accepted animal model to study this core syndrome in vivo. The shortage of animal models limits the ability to uncover the antitumor mechanisms of herbal medicines and to assess the safety profile of the natural products derived from them. This review summarizes the current research on DHZ in cancer in terms of the clinical aspects, molecular landscape, and animal models. This study aims to provide comprehensive insight that can be used for the establishment of a future standardized ZHENG-based cancer animal model.

壮药湿毒清洗剂治疗湿疮(湿热浸淫证)的临床疗效观察

目的:观察壮医湿毒清洗剂治疗湿疮(湿热浸淫证)的临床疗效。方法:选取2021年4月至2022年9月就诊于广西中医药大学附属瑞康医院的湿疮(湿热浸淫证)患者80例,随机分为治疗组及对照组各40例。治疗组用壮药湿毒清洗剂湿敷,对照组用复方黄柏液湿敷,两组均以14 d为1个疗程。1个疗程后,比较两组患者的临床疗效,治疗前后中医证候积分、湿疹面积及严重度指数(EASI)、瘙痒程度评分、皮肤病生活质量指数(DLQI)评分,以及血清免疫球蛋白E(IgE)、肿瘤坏死因子-α(TNF-α)、白介素-4(IL-4)及IL-6表达水平。结果:治疗组和对照组的临床总有效率分别为83.33%(30/36)及54.29%(19/35),治疗组优于对照组(P<0.05);治疗后,两组患者的中医证候积分、EASI评分、瘙痒程度评分、DLQI评分均较治疗前降低(P<0.01),且治疗组患者中医证候积分、EASI评分及DLQI评分均低于对照组(P<0.05);治疗后,两组患者血清中IgE、TNF-α、IL-4、IL-6水平均较治疗前降低(P<0.05),且治疗组患者血清中IgE、TNF-α、IL-6水平均低于对照组(P<0.05)。结论:壮药湿毒清洗剂治疗湿疮(湿热浸淫证)有较好的临床疗效,且可明显降低患者血清中炎症因子IgE、TNF-α、IL-6、IL-4水平,这可能是壮药湿毒清洗剂治疗湿疮(湿热浸淫证)的机制之一。

Efficacy and Safety of Guihuang Formula in Treating Type Ⅲ Prostatitis Patients with Dampness-Heat and Blood Stasis Syndrome: A Randomized Controlled Trial

Objective: To observe the efficacy and safety of Guihuang Formula(GHF) in treating patients with type Ⅲ prostatitis and Chinese medicine syndrome of dampness-heat and blood stasis. Methods: Sixty-six type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome were randomly divided into the treatment group(GHF) and the control group(tamsulosin) using a random number table, with 33 cases each group. The treatment group received GHF twice a day, and the control group received tamsulosin 0.2 mg once daily before bedtime. Patients in both groups received treatment for 6 weeks and was followed up for 2 weeks. The outcomes included the National Institute of Health Chronic Prostatitis Symptom Index(NIH-CPSI) score, Chinese Medicine Symptoms Score(CMSS), expressed prostatic secretions(EPS) and adverse events(AEs). Results: After treatment, the NIH-CPSI total score and domain scores of pain discomfort, urination and quality of life decreased significantly from the baseline in both groups(P<0.05). The CMSS score decreased in both groups(P<0.05). The white blood cell(WBC) count decreased and lecithin body count increased in both groups(P<0.05). GHF showed a more obvious advantage in reducing the pain discomfort and quality of life domain scores of NIH-CPSI, reducing the CMSS score, increasing the improvement rate of the WBC and lecithin body counts, compared with the control group(P<0.05). There were no significant differences in decreasing urination domain score of NIH-CPSI between two groups(P>0.05). In addition, no serious AEs were observed. Conclusion: GHF is effective in treating type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome without serious AEs.(Registration No. ChiCTR1900026966)

自拟祛湿双花饮联合枸地氯雷他定治疗岭南湿疹湿热浸淫型临床观察

目的探讨自拟祛湿双花饮联合枸地氯雷他定治疗岭南湿热浸淫型湿疹的疗效。方法选取2021年1月—2022年12月收治的100例湿疹患者,随机分成对照组和观察组,各50例。对照组选用枸地氯雷他定口服,观察组在对照组基础上加用自拟祛湿双花饮口服。比较2组具体治疗效果。结果治疗后,2组疗效、湿疹面积及严重程度指数(EASI)、瘙痒评分均优于治疗前(P<0.05),且观察组均优于对照组(P<0.05);2组不良反应差异无统计学意义;观察组复发率低于对照组(P<0.05)。结论自拟祛湿双花饮联合枸地氯雷他定治疗岭南地区湿热浸淫型湿疹较单用枸地氯雷他定治疗效果更佳。

Effective Common Chinese Herbal Medicines Used in Treating Chronic Cholecystitis with Liver-Gallbladder Dampness-Heat Syndrome: A Review of Clinical Studies in the Past 10 Years

The objective of the study is to identify the effective common Chinese herbal medicines used in treating chronic cholecystitis with liver-gallbladder dampness-heat syndrome(CCLGDHS) through reviewing relevant clinical studies published in the past 10 years. Data were collected from Science Direct and Chinese National Knowledge Infrastructure. Data screening was carried out for the abstracts and full texts of the data. The top 15 Chinese herbal medicines with the highest occurring frequency were selected, statistically analyzed, and classified by their medicinal properties, actions, and indications according to the Chinese Pharmacopoeia 2015 edition. The top 15 effective common Chinese herbal medicines comprise Chai Hu, Huang Qin, Jin Qian Cao, Bai Shao, Yin Chen, Yu Jin, Chuan Lian Zi, Yan Hu Suo, Zhi Shi, Ban Xia, Bai Zhu, Pu Gong Ying, Gan Cao, Zhi Zi, and Qing Pi. The predominant natures were cold, cool, and warm. This combination can clear stagnant heat, warm Yang, and regulate Qi dynamics. In addition, bitter, pungent, and sweet were the predominant flavors. They can clear dampness-heat, regulate Qi dynamics to relieve cramps and pain, as well as tonify the deficiency. Along with entering the liver and gallbladder meridians, these herbal medicines also entered the spleen, stomach, and lung meridians to prevent potential disease transmission. The combinatorial medicinal actions of the effective common Chinese herbal medicine highlight the importance of the holistic concept of traditional Chinese medicine when treating CCLGDHS. In addition, the inclusion of activating blood to promote blood circulation, relieving cramps and alleviating pain, and tonifying the spleen and stomach represents a new finding in the treatment principle for CCLGDHS.

Haoqin Qingdan Decoction(蒿芩清胆汤)and Ribavirin Therapy Downregulate CD14 and Toll-Like Receptor 4 in Febrile Disease with Dampness-Heat Syndrome in A Mouse Model

Objective： To evaluate the effect of Chinese medicine Haoqin Qingdan Decoction （蒿芩清胆汤, HQD） for febrile disease dampness-heat syndrome （FDDHS）. Methods： Forty mice were divided into four groups, including normal control, FDDHS （induced by Radix et Rhizoma Rhei recipe and influenza virus A1 FM1 model）, HQD, and the ribavirin groups （10 in each）. The normal control and FDDHS groups were administered normal saline. HQD and the ribavirin groups were administered HQD and ribavirin intragastrically once daily at a dose of 64 g/（kg.d） and 0.07 g/（kg.d）, respectively for 7 days. Lethargy, rough hair, diarrhea, tongue color and sole color were evaluated for pathological changes in morphology. The tongue and lung tissues were collected for histology. The CD14 and toll-like receptor 4 （TLR4） expression levels were measured using real-time quantitative polymerase chain reaction. Results： More than 80% of the FDDHS mice showed hypokinesia and lethargy, and pathological changes associated with rough hair, diarrhea, tongue color and sole color. With advanced treatment for 7 days, the thick greasy tongue fur of the HQD and ribavirin groups were thinner than that of the FDDHS group （P〈0.05）, and it was the thinnest in the ribavirin group as compared with that in other groups （P〈0.05）. The CD14 and TLR4 expression levels in the lung tissues of HQD and ribavirin groups significantly delined compared with the model group （P〈0.05 or P〈0.01）. CD14 was down-regulated more remarkably in the HQD group compared with the ribavirin group （P〈0.05）, whereas the converse was true with TLR4 （P〈0.05）. Conclusions： We established a FDDHS mouse model showing systemic clinical symptoms. Both HQD and ribavirin can inhibit the expression of CD14 and TLR4 in FDDHS mice, while the effect of ribavirin might be much more violent. The expression changes of CD14 and TLR4 consistently refers to lipopolysaccharide, the commonly and hotly inducing factor in FDDHS.

寒、热方剂对束缚水浸应激型胃溃疡寒、热证模型大鼠血清TXB_2、NT含量的影响

目的:探讨水浸应激型胃溃疡寒、热证模型的形成机制及寒、热方剂对该模型的作用机理及其方证相应的客观规律。方法:采用寒、热因素结合水浸应激法建立大鼠胃溃疡寒、热证模型,采用酶联免疫法检测受试大鼠血栓素B2(TXB2)和神经降压素(NT)的含量。结果:各模型组与空白组相比,TXB2含量均有不同程度的升高,且差异显著(P<0.01)。且水浸应激型胃溃疡寒、热证之病证结合模型较单证、单病模型TXB2含量明显升高。各模型组与空白组比较NT含量均明显降低,有显著性差异(P<0.05或P<0.01)。但各模型组间比较无明显差异。大黄黄连泻心汤、理中丸可分别显著降低热模型组、寒模型组TXB2含量,升高NT含量(P<0.05或P<0.01)。结论:通过"方证相应"理论验证了"病证结合"胃溃疡寒、热证模型的可行性,大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过降低血清TXB2、升高血清NT的含量而实现。

气压联合热冷水交替浸浴治疗脑卒中肩手综合征的疗效

目的:观察间歇性气压治疗联合热冷水交替浸浴治疗脑卒中肩手综合征Ⅰ期的临床疗效。方法:将脑卒中肩手综合征Ⅰ期患者72例随机分为观察组和对照组各36例,2组患者均进行神经内科常规治疗及运动康复训练,观察组加用间歇性气压治疗联合热冷水交替浸浴治疗。治疗前后进行疼痛评定(VAS)、手部肿胀测量、运动功能评定(FMA)、日常生活活动能力指数(BI)评定。结果:治疗4周后,2组VAS评分及手部肿胀体积均较治疗前明显下降(P<0.05,0.01),且观察组更低于对照组(P<0.01);治疗后2组FMA及BI评分均较治疗前明显升高(P<0.05,0.01),且观察组更高于对照组(P<0.01)。结论:间歇性气压治疗联合热冷水交替浸浴可明显减轻肩手疼痛、肿胀,提高上肢运动功能,改善日常生活活动能力,是治疗脑卒中肩手综合征的有效方法。

寒、热方剂对水浸应激型胃溃疡寒、热证模型大鼠胃组织SOD活性、MDA含量的影响

目的:探讨水浸应激型胃溃疡寒、热证模型的自由基代谢机制及寒、热方剂对该模型的自由基代谢的影响,阐明其方证相应的客观规律。方法:采用寒因素(冰水,0.3mol/LNaOH液)、热因素(8%干辣椒粉的60%乙醇混悬液)分别结合水浸应激法,建立实验性大鼠胃溃疡寒、热证模型。采用黄嘌呤氧化酶法(羟氨法)、硫代巴比妥酸法检测胃组织中SOD活力及MDA含量。结果:SOD方面,水应激胃溃疡组、水应激胃溃疡寒证组、水应激胃溃疡热证组与空白比较活力下降,有显著性差异(P<0.01),但寒证与热证间比较无明显差异。MDA方面,水应激胃溃疡组、水应激胃溃疡热证组、单纯热证组与与空白比较含量明显升高,有显著性差异(P<0.01),且以水应激胃溃疡热证组升高最为显著。大黄黄连泻心汤、理中丸可选择性针对相应的寒性、热性模型体现出对自由基代谢的调节作用。结论:通过"方证相应"理论验证了"病证结合"胃溃疡寒、热证模型的可行性。水浸应激型胃溃疡寒、热证模型存在自由基代谢异常。MDA可作为水浸应激型胃溃疡热证的微观指征。

五苓散冷热浸泡结合康复训练对脑卒中后肩手综合征患者的影响

目的探讨免煎中药加味五苓散冷热浸泡结合康复训练对脑卒中后肩手综合征患者的影响。方法将入选的病例80例随机分成常规组和观察组各40例。常规组按神经内科常规治疗护理、良肢位设计、针灸、康复训练;观察组在常规组基础上,采用免煎中药加味五苓散冷热交替浸泡患手,并同时对患手进行按摩、拍打、活动掌手指关节,治疗4周后评定疗效。结果观察组消肿止痛效果、上肢运动功能与日常生活活动能力与常规组比较,差异有显著意义(P<0.05)。结论免煎中药加味五苓散冷热浸泡结合康复训练,能提高脑卒中后肩手综合征患者康复治疗的效果。

大黄黄连泻心汤、理中丸对消炎痛型及水浸应激型胃溃疡寒热证模型大鼠血清NO和ET含量的影响

目的探讨消炎痛型及水浸应激型胃溃疡中医寒热证模型的形成机制,以及寒热方剂对胃溃疡寒热证模型的基本作用机理和方证相应的客观规律。方法采用寒热因素结合腹腔注射消炎痛法和水浸应激法,分别建立大鼠胃溃疡寒、热证模型,用酶联免疫法检测大黄黄连泻心汤、理中丸对受试大鼠血清中NO和ET含量的影响。结果 NO在水浸应激型胃溃疡和消炎痛型溃疡寒证中含量降低,在热证中含量明显升高(P<0.05或P<0.01)。大黄黄连泻心汤可显著降低热性溃疡组大鼠血清NO的含量,理中丸可显著升高寒性溃疡组大鼠血清NO的含量(P<0.05或P<0.01)。水浸应激型和消炎痛型溃疡寒证中ET含量升高,热性方剂理中丸能降低ET含量。结论 通过"方证相应"理论验证了"病证结合"的胃溃疡寒热模型复制成功。大黄黄连泻心汤对水浸应激型胃溃疡热证、理中丸对水浸应激型胃溃疡寒证的预防作用,及二者对消炎痛型胃溃疡的治疗作用,可通过调节大鼠血清中NO的水平,降低ET的含量而实现。

凉血解毒利湿汤治疗急性湿疹(湿热浸淫证)的临床观察

目的观察凉血解毒利湿汤治疗急性湿疹(湿热浸淫证)的疗效及对血清IgE、肿瘤坏死因子-α(TNF-α)与白介素-4(IL-4)表达的影响。方法 80例患者按照随机数字表法分为治疗组和对照组各40例。对照组给予口服西替利嗪治疗,治疗组给予口服西替利嗪和凉血解毒利湿汤加减治疗。比较两组患者治疗后的临床疗效和体征、症状、舌象、脉象积分,血清Ig E、TNF-α和IL-4炎性细胞因子变化及不良反应。结果治疗组的总有效率92.50%优于对照组的72.50%(P<0.05)。治疗后两组体征、症状、舌象、脉象积分与治疗前比较均降低(均P<0.05),且治疗组优于对照组(均P<0.05)。治疗后,两组血清IgE、TNF-α、IL-4均低于治疗前(均P<0.05),且治疗组低于对照组(均P<0.05)。两组均出现大便稀、胃痛、口干、嗜睡、乏力等轻微不良反应,差别不大(P>0.05)。结论凉血解毒利湿汤治疗急性湿疹(湿热浸淫证)可减轻炎症反应,提高临床疗效,减少中医证候积分,不良反应轻。

大黄黄连泻心汤、理中丸对消炎痛型及水浸应激型胃溃疡寒热证模型大鼠胃组织EGF、TGF-α含量的影响

目的:探讨消炎痛型、水浸应激型胃溃疡中医寒热证模型的形成机制及寒热方剂对胃溃疡寒热证模型的基本作用机理和方证相应的客观规律。方法:采用寒热因素结合腹腔注射消炎痛和水浸应激法,分别建立大鼠胃溃疡寒、热证模型,用酶联免疫法检测大黄黄连泻心汤、理中丸对受试大鼠胃组织中EGF和TGF-α含量的影响。结果:各模型组大鼠胃组织EGF和TGF-α含量显著降低,与空白组比较具有显著性差异(P<0.05或P<0.01);大黄黄连泻心汤可显著升高热性溃疡组大鼠胃组织EGF和TGF-α的含量,理中丸可显著升高寒性溃疡组大鼠胃组织EGF和TGF-α的含量(P<0.05或P<0.01)。结论:通过"方证相应"理论验证了"病证结合"的胃溃疡寒热模型复制成功,大黄黄连泻心汤可以治疗热性消炎痛型胃溃疡,预防热性水浸应激型胃溃疡的形成;理中丸可以治疗寒性消炎痛型胃溃疡,预防寒性水浸应激型胃溃疡的形成,均可通过升高大鼠胃组织中EGF和TGF-α的含量而实现。

改良冷热中药交替浸泡疗法对脑卒中后肩手综合征的疗效观察

目的:观察改良冷热中药交替浸泡疗法对脑卒中后肩手综合征的临床疗效。方法:将80例脑卒中的患者随机分为治疗组与对照组,两组均接受常规治疗及康复训练,对照组进行冷热交替浸泡治疗,治疗组进行改良的冷热中药交替浸泡的疗法。1次/d,20min/次,两组疗程均为4周。两组患者于治疗前后分别进行视觉模拟评分(VAS)、简化Fugl-Meyer运动功能评测、表面肌电测试。结果:对照组与治疗组在治疗前的各项观察指标相比较均无统计学意义(P>0.05)。治疗4周后,治疗组上肢及手的运动功能改善较对照组明显(P<0.05),治疗组腕手部疼痛的视觉模拟评分较对照组改善明显(P<0.05),治疗组表面肌电旋前圆肌肌力较对照组增强程度大(P<0.05)。结论:改良冷热中药交替浸泡对脑卒中后肩手综合征的疗效明显优于仅进行冷热交替浸泡的治疗方法。新型中西医结合的康复治疗方法对于脑卒中后肩手综合征的康复疗效更优。

大黄黄连泻心汤、理中丸对水浸应激型胃溃疡寒热证模型大鼠脑组织5-HT和NE含量的影响

目的:探讨水浸应激型胃溃疡中医寒热证模型的形成机制及寒热方剂对胃溃疡寒热证模型的基本作用机理和方证相应的客观规律。方法:采用寒热因素结合水浸应激法,建立大鼠胃溃疡寒、热证模型,用酶联免疫法检测大黄黄连泻心汤、理中丸对受试大鼠脑组织5-HT和NE含量的影响。结果:寒水与空白、热水组比较,5-HT含量升高明显(P<0.01);热水与空白、寒水、单水组比较NE含量明显升高,有显著性差异(P<0.05或P<0.01);理中丸能够降低寒性溃疡组5-HT含量,大黄黄连泻心汤能够降低热性溃疡组NE含量。结论:通过"方证相应"理论验证了"病证结合"的胃溃疡寒热模型复制成功;热性方剂理中丸预防水浸应激型溃疡寒证的作用机制可能与降低5-HT含量有关;寒性方剂大黄黄连泻心汤预防水浸应激型溃疡热证的作用机制可能与减少NE含量有关。

大黄黄连泻心汤、理中丸对模型大鼠胃组织PGE_2含量的影响

目的:探讨消炎痛型及水浸应激型胃溃疡中医寒热证模型的形成机制及寒热方剂对胃溃疡寒热证模型的基本作用机理和方证相应的客观规律。方法:采用寒热因素结合腹腔注射消炎痛和水浸应激法,分别建立大鼠胃溃疡寒、热证模型,用酶联免疫法检测大黄黄连泻心汤、理中丸对受试大鼠胃组织PGE2含量的影响。结果:各模型组大鼠胃组织PGE2含量显著降低,与空白组比较具有显著性差异(P<0.05或P<0.01);大黄黄连泻心汤可显著升高热性溃疡组大鼠胃组织PGE2的含量,理中丸可显著升高寒性溃疡组大鼠胃组织PGE2的含量(P<0.05或P<0.01)。结论:通过"方证相应"理论验证了"病证结合"的胃溃疡寒热模型复制成功,大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过升高大鼠胃组织PGE2的含量而实现。

病证结合制备寒凝血瘀型子宫内膜异位症大鼠模型

目的:制备具有中医"寒凝血瘀"表现的子宫内膜异位症大鼠模型,并进行观察研究。方法:采用自体移植手术加冰水浸泡法制备大鼠模型。将SD雌性大鼠的子宫内膜片段移植于大鼠腹壁内侧面;对照组只作腹壁切开术,而不作内膜移植。分别于造模后4周、6周、8周各随机取9只,观察大鼠中医临床表征、移植内膜外观,取异位内膜行病理学观察,腹主动脉取血,分别行血液流变学和凝血功能检测。结果:移植物大体呈小囊状,色白透明或暗红,显微镜下小囊具有子宫内膜的基本组织结构,模型动物具有中医"寒凝血瘀"的临床症状及子宫内膜异位症的病理改变。结论:本方法简单可靠,模型成功率高,对中医药治疗子宫内膜异位症的研究有应用价值。

针灸联合桂枝汤泡洗治疗中风恢复期肩手综合征临床观察

目的 探讨针灸联合桂枝汤泡洗治疗中风恢复期肩手综合征(SHS)的临床疗效。方法 前瞻性非随机选取医院康复医学科2016年1月至2018年6月收治的中风恢复期SHS患者96例,按患者意愿分为对照组(41例)和观察组(55例)。两组患者均予针灸治疗,观察组患者加用加减桂枝汤泡洗。结果 观察组总有效率为94.55%,显著高于对照组的75.61%(P<0.05);与治疗前比较,两组患者治疗后的视觉模拟评分(VAS)及内皮素-1(ET-1)、缓激肽(BK)水平均显著降低,Fugl-Meyer运动功能评定量表(FMA)评分、降钙素基因相关肽(CGRP)、一氧化氮(NO)水平均显著升高,且观察组患者上述指标改善程度均显著优于对照组(P<0.05);两组均未发生严重不良反应。结论 针灸联合桂枝汤泡洗治疗中风恢复期SHS,可缓解疼痛,提高上肢运动功能,改善其各项实验室指标。

哈维氏弧菌和白斑综合症病毒对墨吉明对虾仔虾的致病性

研究了不同浓度哈维氏弧菌与白斑综合症病毒单独、合并浸泡感染,以及不同温度和盐度条件下单独、合并浸泡感染对墨吉明对虾仔虾死亡情况和WSSV携带量的影响。结果表明:高浓度哈维氏弧菌浸泡感染仔虾,48 h死亡率可达100%,高浓度WSSV浸泡感染仔虾,96 h死亡率可达97%;盐度突变可以降低弧菌与病毒浸泡感染对仔虾的致病性;高温条件下(32℃)弧菌对仔虾的致病性较强,低温条件下(26℃)病毒对仔虾的致病性较强;盐度26、水温26℃条件下,合并感染组死亡率较WSSV单独感染组低;哈维氏弧菌感染可以抑制WSSV在对虾体内的复制。