Objective： To study the toxicity features of high glucose on the endothelial cell cycle and the influence of Dan Gua-Fang , a Chinese herbal compound prescription, on the reproductive cycle of vascular endothelial ce...Objective： To study the toxicity features of high glucose on the endothelial cell cycle and the influence of Dan Gua-Fang , a Chinese herbal compound prescription, on the reproductive cycle of vascular endothelial cells cultivated under a high glucose condition; to reveal the partial mechanisms of Dan Gua- Fang in the prevention and treatment of endothelial injury caused by hyperglycemia in diabetes mellitus （DM）; and offer a reference for dealing with the vascular complications of DM patients with long-term high blood glucose. Methods： Based on the previous 3-（4,5）-dimethylthiahiazo （z-yl）-3-5-diphenytetrazoliumromide （MI-I-） experiment, under different medium concentrations of glucose and Dangua liquor, the endothelial cells of vein-304 （ECV-304） were divided into 6 groups as follows： standard culture group （Group A, 5.56 mmol/L glucose）; 11300 herb-standard group （Group B）; high glucose culture group （Group C, 16.67 mmol/L glucose）; 1/150 herb-high glucose group （Group D）; 1/300 herb-high glucose group （Group E）; and 1/600 herb-high glucose group （Group F）. The cell cycle was assayed using flow cytometry after cells were cultivated for 36, 72 and 108 h, respectively. Results： （1） The percentage of cells in the Go/G1 phase was significantly increased in Group C compared with that in Group A （P〈0.05）, while the percentage of S-phase （S%） cells in Group C was significantly reduced compared with Group A （P〈0.05）; the latter difference was dynamically related to the length of growing time of the endothelial cells in a high glucose environment. （2） The S% cells in Group A was decreased by 30.25% （from 40.23% to 28.06%） from 36 h to 72 h, and 12.33% （from 28.06% to 24.60%） from 72 h to 108 h; while in Group C, the corresponding decreases were 23.05% and 21.87%, respectively. The difference of S% cells between the two groups reached statistical significance at 108 h （P〈0.05）, （3） The percentage difference of cells in the Gz/M phase between Group C and Group A was statistically significant at 72 h （P〈0.01）. （4） 1/300 Dan Gua-Fang completely reversed the harmful effect caused by 16.67 mmol/L high glucose on the cell cycle; moreover it did not disturb the cell cycle when the cell was cultivated in a glucose concentration of 5.56 mmol/L. Conclusions： High glucose produces an independent impact on the cell cycle. Persistent blocking of the cell cycle and its arrest at the Go/G1 phase are toxic effects of high glucose on the endothelial cell cycle. The corresponding variation of the arrest appears in the S phase. 1/300 Dan Gua-Fang completely eliminates the blockage of high glucose on the endothelial cell cycle.展开更多
Objective：To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang（丹瓜方）,a Chinese herbal compound prescription on endothelial cells of vein（ECV304） cultivated in mediums of different glucose concentr...Objective：To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang（丹瓜方）,a Chinese herbal compound prescription on endothelial cells of vein（ECV304） cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions,in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus. Methods：The research was designed as three stages.The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums,which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine.Results：（1） Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums.（2） Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting ＂pseudopod-like＂ structure formation in cultivated ECV304,but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang.（3） Colchicine reduced and even vanished the ＂pseudopod-like＂ structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang.The ＂pseudopod- like＂ structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed, but it was not the case in hyperglycemia only without Dan Gua-Fang groups.（4） Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine.The cell revived to its normal state fast after colchicine was removed.Conclusion：Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.展开更多
基于嵌入式Internet技术Web管理技术,针对各种设备域网络DAN(Device Area Network)的一致化网络管理,提出了一个对异类设备域网络DAN进行一致管理的Web模型,解决了家庭、企业设备环境中异类网络的一致性管理、Web化管理等问题。该模型...基于嵌入式Internet技术Web管理技术,针对各种设备域网络DAN(Device Area Network)的一致化网络管理,提出了一个对异类设备域网络DAN进行一致管理的Web模型,解决了家庭、企业设备环境中异类网络的一致性管理、Web化管理等问题。该模型是一个以嵌入式设备网关和嵌入式网络网关为主框架的四层结构,集成了Java技术、SNMP协议(Simple Network Management Protocol)、WBDM(Web-based Device Management)管理模型中的EWS(Embedded Web Server)等技术,形成了一个开放的、通用性强的、跨平台的Web管理框架,为嵌入式系统管理员在任何时间和任何地点提供了友好的、平台无关的、易于使用的远程监控嵌入式设备的方法。展开更多
基金Supported by the Post-doctoral Science Funds of China(No. 20070410128)CHEN Ke-ji Development Fund of Integrative Medicine(No.CKJ2006001)Science Fund of Health Department of Fujian Province,China(No.WZY0618)
文摘Objective： To study the toxicity features of high glucose on the endothelial cell cycle and the influence of Dan Gua-Fang , a Chinese herbal compound prescription, on the reproductive cycle of vascular endothelial cells cultivated under a high glucose condition; to reveal the partial mechanisms of Dan Gua- Fang in the prevention and treatment of endothelial injury caused by hyperglycemia in diabetes mellitus （DM）; and offer a reference for dealing with the vascular complications of DM patients with long-term high blood glucose. Methods： Based on the previous 3-（4,5）-dimethylthiahiazo （z-yl）-3-5-diphenytetrazoliumromide （MI-I-） experiment, under different medium concentrations of glucose and Dangua liquor, the endothelial cells of vein-304 （ECV-304） were divided into 6 groups as follows： standard culture group （Group A, 5.56 mmol/L glucose）; 11300 herb-standard group （Group B）; high glucose culture group （Group C, 16.67 mmol/L glucose）; 1/150 herb-high glucose group （Group D）; 1/300 herb-high glucose group （Group E）; and 1/600 herb-high glucose group （Group F）. The cell cycle was assayed using flow cytometry after cells were cultivated for 36, 72 and 108 h, respectively. Results： （1） The percentage of cells in the Go/G1 phase was significantly increased in Group C compared with that in Group A （P〈0.05）, while the percentage of S-phase （S%） cells in Group C was significantly reduced compared with Group A （P〈0.05）; the latter difference was dynamically related to the length of growing time of the endothelial cells in a high glucose environment. （2） The S% cells in Group A was decreased by 30.25% （from 40.23% to 28.06%） from 36 h to 72 h, and 12.33% （from 28.06% to 24.60%） from 72 h to 108 h; while in Group C, the corresponding decreases were 23.05% and 21.87%, respectively. The difference of S% cells between the two groups reached statistical significance at 108 h （P〈0.05）, （3） The percentage difference of cells in the Gz/M phase between Group C and Group A was statistically significant at 72 h （P〈0.01）. （4） 1/300 Dan Gua-Fang completely reversed the harmful effect caused by 16.67 mmol/L high glucose on the cell cycle; moreover it did not disturb the cell cycle when the cell was cultivated in a glucose concentration of 5.56 mmol/L. Conclusions： High glucose produces an independent impact on the cell cycle. Persistent blocking of the cell cycle and its arrest at the Go/G1 phase are toxic effects of high glucose on the endothelial cell cycle. The corresponding variation of the arrest appears in the S phase. 1/300 Dan Gua-Fang completely eliminates the blockage of high glucose on the endothelial cell cycle.
基金Supported by the China Postdoctoral Science Foundation(No. 20070410128)CHEN Ke-ji Development Fund of Integrative Medicine(No.CKJ2006001)Science Foundation of Health Department,Fujian Province(No.WZY0618)
文摘Objective：To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang（丹瓜方）,a Chinese herbal compound prescription on endothelial cells of vein（ECV304） cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions,in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus. Methods：The research was designed as three stages.The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums,which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine.Results：（1） Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums.（2） Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting ＂pseudopod-like＂ structure formation in cultivated ECV304,but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang.（3） Colchicine reduced and even vanished the ＂pseudopod-like＂ structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang.The ＂pseudopod- like＂ structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed, but it was not the case in hyperglycemia only without Dan Gua-Fang groups.（4） Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine.The cell revived to its normal state fast after colchicine was removed.Conclusion：Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.
文摘基于嵌入式Internet技术Web管理技术,针对各种设备域网络DAN(Device Area Network)的一致化网络管理,提出了一个对异类设备域网络DAN进行一致管理的Web模型,解决了家庭、企业设备环境中异类网络的一致性管理、Web化管理等问题。该模型是一个以嵌入式设备网关和嵌入式网络网关为主框架的四层结构,集成了Java技术、SNMP协议(Simple Network Management Protocol)、WBDM(Web-based Device Management)管理模型中的EWS(Embedded Web Server)等技术,形成了一个开放的、通用性强的、跨平台的Web管理框架,为嵌入式系统管理员在任何时间和任何地点提供了友好的、平台无关的、易于使用的远程监控嵌入式设备的方法。
