Informatics-based prediction of molecular networks targeted by active ingredients in Danshen (Salvia miltiorrhiza)

Salvia miltiorrhiza (Chinese, Danshen) is one of the most famous traditional herbs, and has been used to treat cardiovascular disease. Despite the wide application of Danshen in China, the mechanisms of its bioactive components are poorly understood. The present study used bioinformatics to identify possible mechanisms by which Danshen treats cardiovascular disease. Possible human protein targets of Danshen were identified in the PubChem database, possible human gene targets of cardiovascular disease were identified in the NCBI database, and then both sets of targets were analyzed using Ingenuity Pathway Analysis to predict molecular networks affected by Danshen in cardiovascular disease. The results suggest that signaling proteins affected by Danshen in cardiovascular disease, which include FASN, PAFAH1B2, PLA2G7, PAFAH1B3 and IL1B, are involved primarily in LXR/RXR activation, atherosclerosis signaling, hepatic fibrosis/hepatic stellate cell activation, acute phase response signaling. The main networks affected by Danshen are predicted to involve in cellular movement, immune cell trafficking, hematological system development and function;DNA replication, recombination, and repair, cancer, hematological disease;cardiovascular disease, organismal injury and abnormalities, tissue morphology.These results identify several specific proteins and pathways that may be affected by Danshen in cardiovascular disease, and they illustrate the power of integrative bioinformatics and chemical fragment analysis for focusing mechanistic studies.

Tyrosine Aminotransferase Gene (SmTAT) Revealed Genetic Diversity and Phylogeny of Cultivated Danshen (Salvia miltiorrhiza) Populations

Chinese traditional medicine Danshen is the radix of the perennial herbs of Salvia miltiorrhiza Bunge, which has a variety of pharmacological effects and is traditionally and extensively applied clinically to treat cardiovascular disorders. In this research, the genomic genes for tyrosine aminotransferase (TAT) of 38 cultivated populations of Danshen in China were cloned and bioinformatic analyses were conducted to reveal its genetic diversity and phylogeny. The full-length SmTAT was 2296 - 2444 bp including 6 exons (encoding 411 amino acids) and 5 introns. Overall, the SmTAT genes in cultivated Danshen populations are highly conserved with a relative low level of genetic diversity. The spliced exons (1236 bp) had 23 SNP variations with a rate of 1.86%, of which 22 occurred in the white flower S. miltiorrhiza Bge.f.alba population (W-SCHY-W-1) and led to 5 amino acid variations. The entire 290 SNP variations with a rate of 24% in the 5 introns occurred exclusively in W-SCHY-W-1. Phylogenetic trees based on the full-length, combined introns, the spliced exons, and the deduced amino acid sequences of SmTAT all showed a two-clade basic structure with W-SCHY-W-1 uniquely standing alone. The SmTAT gene of the white flower population (W-SCHY-W-1) is unique and especially rich in variations. The first time clarified genomic SmTAT gene structure and genetic diversity in cultivated Danshen populations laid an excellent foundation for further studies on the biosynthesis of bioactives and the molecular breeding of Danshen as well as in plant tyrosine metabolism.

丹参(Salvia miltiorrhiza)中一对具有新奇结构的立体异构体的结构测定

为了进一步弄清丹参的化学成分，应用化学和色谱分离方法从丹参中分离到一个白色粉末固体，经综合运用先进的波谱分析手段，包括１Ｈ－１ＨＣＯＳＹ、１Ｈ－１３ＣＣＯＳＹ、ＣＯＬＯＣ、ＮＯＥ等二维核磁共振谱确定了它们的化学结构为一对具有螺环缩酮内酯结构的新二萜类立体异构体化合物．

Progress in research into the mechanism of Radix salviae miltiorrhizae in treatment of acute pancreatitis

BACKGROUND: Acute pancreatitis (AP) is a common acute abdomen with high mortality, multiple complications and complicated causes. There is no effective therapy for AP. Radix salviae miltiorrhizae (Danshen), a traditional herbal medicine, has a low price and a wide range of clinical applications. It is effective to promote blood flow, eliminate stagnancy, and relieve pain. It is also found to be effective in treating AP. We reviewed the progress in research into the mechanism of Radix salviae miltiorrhizae in treatment of AP. DATA SOURCES: An english-language literature search was conducted using MEDLINE (1988-2005) on Radix salviae miltiorrhizae (Danshen) and acute pancreatitis. RESULTS: The mechanisms of Danshen in the treatment of AP include improvement of microcirculatory disturbances; elimination of oxygen free radicals; modulation of the metabolism of lipid inflammatory mediator; and blocking of calcium inflow and prevention of calcium overload.CONCLUSION: Danshen can effectively reduce the mortality and complications of AP.

Short term effect of Salvia miltiorrhiza in treating rat acetic acid chronic gastric ulcer and long term effect in preventing recurrence

AIM To study the short term effect of Danshen ( Salvia miltiorrhiza ) on acetic acid induced chronic gastric ulcer in rats and its long term effect in preventing recurrence. METHODS Rats with acetic acid induced gastric ulcer were treated with Danshen and cimetidine for 30 days. Traditional gastric mucosal auto radiography and 3H TdR incorporation into gastric mucosa in vitro were employed to study the effects of Danshen in rat acetic acid induced chronic gastric ulcer, including ulcer index (UI), ulcer inhibitory rate (IR) and label rate (LR). RESULTS On the day 5, 30 and 126 of ulcer making, the UI in the Danshen group was obviously lower than that in the cimetidine group and the control group (42 3±3 9, 3 6±1 2, 4 4±2 3; 49 1±3 6, 5 9±1 4, 9 2±1 3; 61 0±3 8, 8 9±2 5, 12 4±2 4, respectively, P <0 01), the IR (%) in the Danshen group was obviously higher than that in the cimetidine group (31, 59, 64 8; 19, 33, 26, respectively), and the LR in the Danshen group was obviously higher than that in the cimetidine group and the control group (10 0±0 5, 16 2±0 8, 15 0±0 6; 9 0±0 5, 13 9±0 6, 10 8±0 7; 6 5±0 7, 10 1±0 5, 8 0±0 7, respectively, P <0 01). There was no obvious difference in UI in the Danshen group on day 30 as compared with that on day 126. CONCLUSION Danshen is effective in promoting ulcer healing and preventing recurrence. The mechanism of action is to strengthen the gastric mucosal barrier and to promote the gastric mucosal cell proliferation along the edge of the ulcer.

Effect of Salvia Miltiorrhiza on Expression of the MMP-2 9 in Tissue of Early Stage Acute Lung Injury in Rats with Severe Acute Pancreatitis

Objective:To investigate the effect of salvia miltiorrhiza on expression of the MMP-2、9 and TIMP-1、TIMP-2 in tissue of acute lung injury of severe acute pancreatitis(SAP).Methods:MMP-2、9 expression and changes of the lung were measured after the SAP rats were induced by retrograde injection of 5%sodium tauocholate into hepatopancreatic duct.The changes of those parameters were also measured after salvia miltiorrhiza was injected intramuscularly just after induction of SAP.Results:The level of MMP-2、9 in pancreas and lung in SAP group were significantly higher than those in sham;The level of MMP-2、9 in salvia miltiorrhiza group were significantly lower than those in SAP group. Conclusion:MMP-2、9 were overexpressed in Acute lung injury (ALI) induced by SAP, salvia miltiorrhiza downregulates MMP-2、9 expression and decreased injury of lung tissue.

丹参及其制剂治疗冠心病的药理及机制研究新进展

传统中药丹参及其制剂在临床应用范围广泛,对于冠心病(coronary heart disease,CHD)有良好疗效,其脂溶性成分丹参酮ⅡA具有抗动脉粥样硬化、抗心肌缺血、抗心肌纤维化和抑制心肌肥厚等作用,通过作用于Toll样受体/核转录因子-κB(toll like receptor 4/nuclear transcription factor-κB,TLR/NF-κB)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)/NF-κB、磷脂酰肌醇3-激酶/蛋白激酶B(phosphoinositide 3-kinase/protein kinase B,PI3 K/AKT)、雷帕霉素靶蛋白/内皮型一氧化氮合酶(mammalian target of rapamycin/endothelial nitric oxide synthase,mTOR/eNOS)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)/Smad2/3、应激激活蛋白激酶(stressac-tivated pro-tein kinase,SAPK)/MAPK、胞外信号调节蛋白激酶(extracellular regulated protein kinases,ERK)/核转录因子红细胞系2相关因子2(nuclear factor erythroid-2-related factor 2,Nrf2)、胱抑素C/Wnt(cystatin C/Wnt,Cys-C/Wnt)等信号转导通路发挥效应;隐丹参酮通过抑制炎性介质预防早期冠脉粥样硬化。水溶性成分丹酚酸A通过调节PI3 K/Akt、丝氨酸/苏氨酸激酶(serine/threonine kinase,GSK-3β)、c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)和ERK1/2等通路抑制心肌细胞凋亡,降低炎症水平,改善心肌梗死;丹酚酸B通过抑制TLR4和核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3)炎性体表达减轻心肌损伤;丹参素通过抑制Janus酪氨酸蛋白激酶(janus tyrosine protein kinase,JAK)/信号转导及转录激活因子(signal transduction and transcriptional activator,STAT)信号通路改善微循环、抗炎抗氧化、抗内皮损伤和抗血小板聚集。丹参水溶性成分制剂的主要靶点有前列腺素-内过氧化物合酶(prostaglandin-endoperoxidase synthase 2,PTGS2)、半胱氨酸天冬氨酸蛋白酶(Caspase-3)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP9)、MAPKs、TLR4、JUN、STAT3、TLR/NF-κB、PI3 K/Akt、JAK2/STAT3和Nrf2/血红素加氧酶-1(heme oxygenase-1,HO-1)等,具有抗炎、改善血液流变性、保护血管内皮、抗血小板聚集等药理作用;复方丹参滴丸具有扩张冠脉、减少心肌耗氧、增加冠脉流量、增强心肌收缩等作用,还具备用量小、不良反应少,对胃肠刺激小等优点;冠心宁片治疗CHD的靶点包括核受体共激活因子2(nuclear receptor coactivator 2,NCOA2)、一氧化氮合酶2(nitric oxide synthase 2,NOS2)等,主要调控PI3 K/Akt、白细胞介素-17(interleukin-17,IL-17)、低氧诱导因子-1(hypoxic inducible factor-1,HIF-1)等通路;新加丹参饮具有扩张冠脉、增加血流量、改善微循环等作用;冠心丹参方的主要药理活性在于增加冠脉血流量,降低心肌耗氧,从而改善心肌缺血,提高心功能。

Experimental study on the effect of Huangqi and Danshen ultramicro gel on vascular healing of wound in rats based on network pharmacology

Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of Huangqi and Danshen ultrafine granule gel on wound vascular healing in rats by network pharmacology and experimental verification.Methods:Using the TCMSP database to screen the active components of two herbs and their potential targets,to search the target related to vascular injury through the Gene Card database,and to select the gene target with correlation coefficient greater than 10%.Then map the disease related target to the potential target of the compound,to obtain the common target,and to import the information to the String online analysis platform and Cytoscape to make the PPI map,and to carry on the topological analysis,KEGG and GO enrichment analysis through Metascape platform.The expression analysis of the main target genes of network pharmacology was verified by pathological section staining and optical density method to determine the content of vascular endothelial growth factor(VEGF)in tissues,the number of tissue new capillaries,and the wound healing rate.Results:A total of 115 active components and 297 target genes were selected from Huangqi and Danshen,and 123 target genes were intersected with vascular injury,among which IL6,AKT1,INS,VEGFA,TNF,EGF,CASP3,MAPK1,TP53 were the active genes with the highest intersection value.The pathways involved are mainly related to AGE-RAGE signaling pathway in diabetic complications,MAPK signaling pathway,Pathways in cancer,human cytomegalovirus infection,Fluid shear stress and atherosclerosis,Endocrine resistance,foxo signaling pathway etc.We experimentally validated VEGFA based on the primary target of vascular regeneration,the experimental results showed that there was different expression of VEGFA proteins in wound vascular healing process,and the quercetin,daidzein,luteolin,apigenin,tanshinone i of 115 active components were selected as the main compounds acting on the VEGFA.Conclusion:This study systematically reveals the material basis and mechanism of Huangqi and Danshen regulating wound vascular healing through multi-component,multi-target and multi-pathway and provides theoretical and scientific basis for the clinical application of Huangqi and Danshen.

山楂丹参分散片的制备工艺研究

目的考察山楂丹参分散片最佳制备工艺。方法以丹参药材中丹参素钠、山楂药材中金丝桃苷为指标,采用L_(9)(3^(4))正交试验设计,对丹参、山楂提取工艺开展研究;通过对山楂丹参分散片中填充剂、润湿剂、崩解剂的考察,以分散均匀度为考察指标,对山楂丹参分散片处方和工艺进行筛选。结果山楂丹参分散片的最佳制备工艺为取丹参药材,加10倍量水回流提取3次,每次2 h,取山楂药材,用10倍量75%乙醇回流提取2次,每次1 h,分别将药液浓度至1.0 g/mL,并通过AB-8型大孔吸附树脂柱洗脱、浓缩、干燥,将上述丹参、山楂提取物混匀,过80目筛,再分别加入微晶纤维素、硫酸钙、羧甲基纤维素钠、交联羧甲基纤维素钠(过100目筛),混合均匀,以50%乙醇为黏合剂,24目筛制粒,60℃干燥。结论按最佳制备工艺制备的丹参、山楂提取物中丹参素钠、金丝桃苷含量较高;山楂丹参分散片在3 min内分散均匀,各项指标均符合2020年版《中华人民共和国药典》(四部)规定,表明山楂丹参分散片制备工艺稳定可行,筛选处方合理,可进一步研发。

丹参对糖尿病肾病保护作用及机制研究

糖尿病肾病(diabetes nephropathy,DN)是导致终末期肾脏病的首位致病因素,严重威胁患者生命健康。传统中医药在糖尿病肾病的治疗中具有重要潜力作用,丹参作为活血化瘀药代表,具有活血祛瘀、清心除烦之功。经研究发现,丹参含有丹酚酸、丹参酮、挥发油、多糖、含氮类化合物等多种成分,能够通过多途径多靶点改善肾小球超滤过状态和间质纤维化等病理改变,其中丹酚酸类和丹参酮类是丹参发挥糖尿病肾病保护作用的主要生物活性成分,具有降糖、调脂、抗炎、抗氧化应激、改善血流动力学、纠正肠道菌群紊乱、调节自噬水平等多种药理作用。该文参考近年来丹参相关制剂干预糖尿病肾病的临床研究和动物实验,对丹参在糖尿病肾病发生发展过程中起到的作用机制进行归纳总结,以期为临床应用丹参治疗糖尿病肾病提供更好的治疗策略和理论依据。

丹参及其有效成分对心血管系统的药理机制研究进展

中国传统药材丹参因极佳的活血祛瘀、通经止痛、清心除烦等功效历来被广泛用于医疗实践中,尤以在心血管系统中的应用突出。现代研究发现,丹参及其活性成分具有抗凝、降脂、抗血栓形成、抗炎抗氧化应激、降压、保护心肌细胞、减少心肌细胞凋亡率、保护血管和促进血管扩张、改善线粒体功能障碍和微循环、防治心室肥大等作用,使得其对心血管系统疾病有着良好的防治效果。综述丹参及其有效活性成分从抗动脉粥样硬化、保护心肌细胞、保护血管、改善心肌缺血和缺血再灌注损伤、防治高血压和心室肥大等内容,力求为丹参在心血管系统疾病的应用和研究提供参考。

丹参有效成分及药理作用研究进展

丹参是一种多年生植物,属于唇形科、丹参属,是一种遮荫草本植物。唇形科植物丹参的干燥根和根茎在中国通常被称为丹参,作为广泛使用的传统中药有着近2000年悠久历史,具有活血化瘀、通经止痛、清心除烦、凉血消痈之功效。丹参的有效成分复杂,并且具有多种药理作用。丹参的化学成分主要为水溶性的酚酸类以及脂溶性的丹参酮类化合物,该植物及其提取物具有抗炎、抗氧化、抗动脉粥样硬化、抗肿瘤、抗纤维化、糖尿病肾病保护等药理作用。文章参阅近些年文献以及相关研究深入阐述丹参有效成分以及药理作用,以期为丹参临床应用研究提供理论依据。目前关于丹参的药理作用研究虽取得了一定的成果,但对其化学成分及药理机制的研究尚不完善,后续可从细胞、组织、分子生物学、代谢学等多学科、多方面进行深入研究,以期为明确丹参的有效成分及开展丹参有效成分机制和临床应用研究提供理论依据。

丹参品种、产地及栽培历史本草考证

丹参为我国常用大宗中药材,历代本草均有记载,药用历史悠久,功效显著,具有活血止痛、宁心安神的作用,为常用活血化瘀药,临床医疗价值较高,在治疗心肌缺血、改善肝功能、保护肾脏、对抗肿瘤等方面具有较好的效果。查阅文献发现,近年来对丹参的研究大多集中在药理成分、临床等方面,鲜有从古籍文献出发对丹参品种、产地及栽培历史进行研究。通过系统查阅丹参古今文献发现,丹参近缘品种较多,在民间应用和地方入药中同属多种植物的根均作丹参用;在清代以前为野生丹参,清代以后才出现人工栽培丹参,以山东、四川丹参质量最好;各地虽有栽培,但是栽培品种混杂,品质退化严重,质量参差不齐,为此,有必要对丹参的品种、产地及栽培历史进行考证,为丹参正本清源及资源的进一步开发利用提供理论基础和现实依据。

丹参及其药对的药理研究进展

丹参为我国传统中药,常用大宗药材品种之一,用药历史久远,为活血祛瘀、通经止痛的良药,具有抗肿瘤、保护心脑血管、调节女性生殖、抗氧化等药理作用。在临床应用中,丹参常与川芎、红花、葛根、黄芪、大黄、当归等组成药对使用,可增强或扩大疗效。重点对丹参的药理作用及常用丹参药对的药理作用、临床应用进行综述,并对丹参药对使用规律、配伍理论进行探讨,以期为丹参及其药对进一步的开发利用提供参考。

自拟复方丹参方联合芦可替尼治疗骨髓纤维化患者的临床疗效及对其生活质量的影响

目的探究自拟复方丹参方联合芦可替尼治疗骨髓纤维化患者的临床疗效及对其生活质量的影响。方法选取2017年10月—2020年12月期间邢台市第一医院收治的90例骨髓纤维化患者,根据随机数字表法分为对照组和研究组,每组各45例。对照组给予磷酸芦可替尼片治疗,研究组在对照组基础上联合复方丹参方治疗,两组患者均治疗6个月。观察比较两组患者临床疗效、骨髓纤维化改善率、不良反应发生率,治疗前后骨髓增殖性肿瘤总症状评估量表(Myeloproliferative neoplasm symptom assessment form,MPN-SAF-TSS)评分、脾脏大小(脾脏的长径、厚径)、血常规指标[白细胞计数(White blood cell count,WBC)、血小板(Platelet,PLT)、血红蛋白(Hemoglobin,Hb)]及生活质量评分。结果治疗后研究组有效率75.56%(34/45)明显高于对照组55.56%(25/45),差异有统计学意义(P<0.05)。治疗后研究组骨髓纤维化改善率64.44%(29/45)明显高于对照组42.22%(19/45),差异有统计学意义(P<0.05)。治疗后两组患者MPN-SAF-TSS评分均较治疗前降低,差异有统计学意义(P<0.05);且研究组MPN-SAF-TSS评分明显低于对照组,差异有统计学意义(P<0.05)。治疗后两组患者脾脏的长径和厚径均较治疗前减小,差异有统计学意义(P<0.05);且研究组脾脏的长径和厚径均明显小于对照组,差异有统计学意义(P<0.05)。治疗后两组患者WBC、PLT水平均较治疗前降低,Hb水平均较治疗前升高,差异有统计学意义(P<0.05);且研究组WBC、PLT水平均明显低于对照组,Hb水平明显高于对照组,差异有统计学意义(P<0.05)。治疗后两组患者QOL评分均较治疗前升高,差异有统计学意义(P<0.05);且研究组QOL评分明显高于对照组,差异有统计学意义(P<0.05)。治疗期间,研究组不良反应发生率17.78%(8/45)与对照组20.00%(9/45)比较,差异无统计学意义(P>0.05)。结论自拟复方丹参方联合芦可替尼治疗骨髓纤维化可减轻患者临床症状,缩小脾脏,改善血常规指标,降低骨髓纤维化程度,提高生活质量,安全性高。

基于“肺虚络瘀”理论及网络药理学与分子对接探析黄芪-丹参药对治疗肺纤维化作用机制

目的基于“肺虚络瘀”理论及网络药理学与分子对接方法探析中药黄芪-丹参药对配伍治疗肺纤维化的作用机制。方法借助TCMSP,分别以“黄芪”“丹参”为关键词检索药物活性成分及对应靶点,在UniProt蛋白质数据库中将药物成分靶点名称转化为对应基因名;运用OMIM、GeneCards、TTD、DisGeNET等疾病基因数据库,分别检索并筛选肺纤维化疾病的靶点基因。使用venny 2.1在线软件作图工具平台,将药物成分靶点与肺纤维化疾病靶点取交集,获取药物-疾病共同靶点基因,并绘制韦恩图;使用Cytoscape 3.10.0作图软件构建中药-成分-共同靶点-疾病基因网络;再运用String数据库构建其蛋白互作(PPI)网络,同时筛选出核心靶点;运用Metascape数据库对药物-疾病交集靶点进行GO和KEGG富集分析,通过微生信平台对富集结果进行可视化,借助AutoDock 1.5.7软件进行分子对接验证。结果中药黄芪-丹参药对活性成分共85个,核心成分为槲皮素、山柰酚、丹参酮Ⅵ、丹参酮IIA;成分靶点1443个,药物-疾病交集靶点基因共85个,作用于疾病的核心靶点12个;GO富集分析结果分别得到生物过程(BP)1370条,分子功能(MF)91条,细胞组成(CC)60条;KEGG富集分析有169个信号通路;分子对接验证结果显示,小分子配体与蛋白受体之间均有相对稳定的结合力。结论“黄芪-丹参”药对通过槲皮素、山柰酚、丹参酮IIA等多种有效成分,可能通过调控MMP-9、TP-53、TNF、IL-1β、TGF-β1等蛋白表达,从而发挥其抗肺纤维化的作用。

靶细胞提取和高效液相飞行时间质谱联用分析预测丹参中的活性成分

建立靶细胞提取和与高效液相飞行时间质谱联用(HPLC-ESI/TOFMS)分析相结合进行丹参活性成分研究的方法,对丹参中可能的活性成分进行了推测。将靶细胞和丹参样品一起孵育培养,根据活性成分可以与靶细胞膜有特异性的结合或者进入靶细胞内的原理,用HPLC-MS方法对培养后细胞破碎液中的成分进行分析。从加药Raw264.7细胞和Ecv304细胞破碎液中检测到丹参素、原儿茶醛、咖啡酸、丹酚酸D、紫草酸、迷迭香酸及丹酚酸B7种丹参中成分,在加药的HL7702细胞破碎液中检测出丹参素、原儿茶醛、咖啡酸及丹酚酸B4种丹参中的水溶性成分。结果显示:细胞破碎液中检测的成分为丹参在靶细胞中有吸收的成分,本方法可用于预测中药中的活性成分。

替代对照品法用于丹参和复方丹参片含量测定的研究

目的:建立HPLC替代对照品法测定丹参和复方丹参片的含量。方法:选取对羟基苯甲酸甲酯作为丹参和复方丹参片含量测定对照品丹酚酸B的替代对照品,在不同的条件下测定替代对照品相对对照品的校正因子,利用校正因子和替代对照品对羟基苯甲酸甲酯进行丹参和复方丹参片中丹酚酸B的含量测定。结果:丹酚酸B和对羟基苯甲酸甲酯进样量分别在0.09～2.71μg(r=1.0000)和0.015～0.45μg(r=1.0000)内与峰面积呈良好的线性关系(n=7),测得校正因子f=5.2884,用替代对照品测定方法测得回收率为100.5%(n=9)。结论:本文首次在高效液相色谱仪上使用替代对照品测定了丹参和复方丹参片中丹酚酸B的含量,结果证明用替代对照品对于丹参和复方丹参片进行药品质量控制是可行的、实用的。

不同厂家生产的丹参注射液色谱指纹图谱的比对研究

目的:比较不同厂家丹参注射液的质量,并应用对照品对照和高效液相色谱-质谱联用技术对其主要成分进行定性分析。方法:采用Zorbax Extend C18色谱柱,以乙腈-0.05%三氟乙酸水溶液为流动相梯度洗脱,流速为0.8mL.min-1,检测波长为288nm,测定了18个不同厂家生产的丹参注射液的HPLC-UV指纹图谱以及代表性厂家注射液的HPLC-DAD-MS图谱。结果:18个不同厂家生产的丹参注射液在所含化学成分的数目和含量上差异显著;利用对照品对照和HPLC-DAD-MS技术指认了丹参注射液中的15个酚酸类成分。结论:本方法可用于测定不同来源的丹参注射液的色谱指纹图谱,并为丹参注射液的全面质量评价和生产工艺监控提供了可行的方法。

加味丹参饮预处理对心肌细胞内钙超载的延迟保护作用

目的观察加味丹参饮预处理对心肌细胞内钙超载的延迟保护作用。方法将培养72h的大鼠心肌细胞随机分6组,空白组正常培养;正常血清对照组加50%大鼠血清培养;含药血清组加50%含加味丹参饮的药物血清培养;缺氧再给氧组予缺氧3h,再给氧1h。缺氧预处理组、加味丹参饮预处理组分别给予缺氧预处理、加味丹参饮预处理,24h后再予缺氧3h,给氧1h。结果缺氧再给氧组乳酸脱氢酶(LDH)、肌酸激酶(CK)及细胞内钙离子浓度显著升高(P<0.01),缺氧预处理和加味丹参饮预处理组LDH、CK及细胞内钙离子浓度显著低于缺氧再给氧组(P<0.01),与空白组、血清对照组和含药血清对照组相似(P>0.05)。结论加味丹参饮预处理的延迟保护作用可防止心肌细胞内钙离子超载,从而发挥细胞保护作用。