GC/GCMS analysis of the petroleum ether and dichloromethane extracts of Moringa oleifera roots

Objective:To explore the phytochemical constituents from petroleum ether and diehloromethane extracts of Moringa oleifera(M.oleufera)roots using GC/GC—MS.Methods:A total of 5.11 kg fresh and undried crashed root of M.oleifera were cut into small pieces and extracted with petroleum ether and diehloromethane(20 L.each) at room temperature for 2 d.The concentrated extracts were subjected to their GC—MS analysis.Results:The GC-MS analysis of the petroleum ether and diehloromethane extracts of M.oleifern roots,which showed promising biological activities,has resulted in the identification 102 compounds.These constituents belong to 15 classes of compounds including hydrocarbons,fatty acids,esters,alcohols,isolhioeyanate.thiocyanale,pyrazine,aromalics.alkamides.cyanides,steroids,halocompounds.urea and N-hydroxyimine derivatives,unsaturated alkenamides.alkyne and indole.GC/GC-MS studies on petroleum ether extraet of the roots revealed that it contained 39 compounds,belonging to nine classes.Cyclooctasulfur S8 has been isolated as a pure compound from the extract.The major compounds identified from petroleum ether extract were trans-13-clocosene(37.9%).nonacosane(32.6%).cycloartenol(28.6%) nonadecanoic acid(13.9%) and cyclooctasulfur S8(13.9%).Dichloromethane extract of the roots was composed of 63 compounds of which nasimizinol(58.8%) along with oleic acid(46.5%),N—benzyl-N-(7—cyanato heptanamide(38.3%),N—benzyl-N—(1—chlorononyl) amide(30.3%),bis[3—benzyl prop-2-ene]-1-one(19.5%) and N.N-dibeuzyl—2-ene pent 1.5—diamide(11.6%) were the main constituents.Conclusions:This study helps to predict the formula and structure of active molecules which can be used as drugs.This result also enhances the traditional usage of M.oleifera which possesses a number of bioactive compounds.

Bounds for Polynomial’s Roots from Fiedler and Sparse Companion Matrices for Submultiplicative Matrix Norms

We use submultiplicative companion matrix norms to provide new bounds for roots for a given polynomial P(X) over the field C[X]. From a n×n Fiedler companion matrix C, sparse companion matrices and triangular Hessenberg matrices are introduced. Then, we identify a special triangular Hessenberg matrix Lr, supposed to provide a good estimation of the roots. By application of Gershgorin’s theorems to this special matrix in case of submultiplicative matrix norms, some estimations of bounds for roots are made. The obtained bounds have been compared to known ones from the literature precisely Cauchy’s bounds, Montel’s bounds and Carmichel-Mason’s bounds. According to the starting formel of Lr, we see that the more we have coefficients closed to zero with a norm less than 1, the more the Sparse method is useful.

EXTRACTION OF CHARACTERISTIC ROOTS ON BESSEL-NEUMANN’S MIXED EQUATIONS

This paper discusses the problem of the extraction of characteristic roots on Bessel-Neumann's mixed equations. It gives the expressions and the evaluation of the minimum root. The advantage of the method has no use for the table of the multi-figure number Bessel function and it does not need computer but can calculate all the characteristic roots The precision of these roots is still high.

S型控释氮肥运筹对水稻产量形成及氮素利用特征的影响

缓控释肥具有施用次数少、肥料利用率高的特点,S型热固性树脂包膜控释氮肥(S型控释氮肥)受土壤pH影响小、肥料稳定性好。本研究以S型控释氮肥为试材,分别设置了6个水稻品种的响应比较、4种氮肥施用量、5种氮肥施用时期及7种氮肥配施比例的4个盆栽试验,研究其对水稻产量形成、氮素吸收利用等的影响。结果表明:1)品种比较试验中,S型控释氮肥提高了6个水稻品种的产量,增幅在2.95%~18.57%,平均增加12.60%;产量提高的原因是穗数或每穗颖花数的增加;S型缓控释氮肥可显著提高供试水稻成熟期的氮素积累量、氮肥利用率,但氮素籽粒生产效率有所下降。2)控释肥运筹试验中,随着S型控释氮肥施用量和施用比例的增加,武运粳24号的产量呈先增加后下降的趋势,分别以每盆3g纯氮处理和控释氮肥占比80%处理产量增幅最大,作基肥时增产效应更显著;成熟期氮素积累量呈增加和先增加后下降的趋势,分别以每盆4.5 g纯氮处理和控释氮肥占比80%处理增幅最大,作基肥时增幅效应更显著;氮素籽粒生产效率和氮肥利用率呈降低趋势,氮素籽粒生产效率分别以控释氮肥占比0%和控释肥作保花肥最大,氮肥利用率分别以控释氮肥占比80%和控释肥作基肥时最大。3)控释肥对根系性状影响试验中,S型控释氮肥处理提高了武运粳24号水稻栽后30天单株不定根总长、根干重与吸收面积,提高了抽穗期单株不定根总长、根干重和根活力;单株不定根数、不定根总长、根干重、根活力的增加是产量、氮素积累量和氮肥利用率协同提高的根系基础。总之,S型控释氮肥可显著提高水稻的产量和氮肥利用率,作为基肥时最优。每盆3 g纯氮处理、控释氮肥占比80%左右时产量、成熟期氮素积累量、氮肥利用率协同增加,与其栽后30天、抽穗期根系性状优化有密切关系。

Culture Street—The Hub of Tianjin's Cultural Roots

Culture Street is the place which no visitor to Tianjin will want to miss. There one can find Qing-style mansions stretching for more than six hundred metres, displays of antiques and per-formances of well-preserved cultural activities. The street is located at the northeastern corner of old Tianjin city where the roots of the city’s history and culture lie. Two hundred years ago when Tianjin was a small port, Tianhou (Goddess of the Temple) was already there. Merchants and those who were traveling away from home by sea would all go to the temple to ask for the god-

基于S-CAD方法的安全生产治本攻坚政策评估

国务院安委会牵头发布《安全生产治本攻坚三年行动方案(2024—2026年)》(简称“总政策”),为此,各部门制定了31个部门子方案及部分省级行动实施方案(简称“子政策”),从而形成“1+31+N”的安全生产治本攻坚总体方案。论文运用S-CAD方法和NVivo12 Plus软件,对上述政策文本开展量化政策评估。通过系统梳理从目标到结果的逻辑链条,研究发现,总政策与子政策在目标与手段、手段与价值观/立场存在逻辑上的高度一致性,手段对目标的实现上存在较强充分性。在行动方案实施过程中,需要聚焦总政策与子政策的衔接性、政策工具选择的多样性、治理目标的一致性,树立本质安全理念,坚持政府间多部门以及政府、市场与社会多主体双向协同治理,采取行政、市场、法律、社会等多种政策工具,建立事前预防型公共安全治理模式,推进高质量发展和高水平安全良性互动,实现安全生产治理体系和治理能力现代化。

Molecular regulatory mechanism of tooth root development

The root is crucial for the physiological function of the tooth, and a healthy root allows an artificial crown to function as required clinically. Tooth crown development has been studied intensively during the last few decades, but root development remains not well understood. Here we review the root development processes, including cell fate determination, induction of odontoblast and cementoblast differentiation, interaction of root epithelium and mesenchyme, and other molecular mechanisms. This review summarizes our current understanding of the signaling cascades and mechanisms involved in root development. It also sets the stage for de novo tooth regeneration.

Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease Effects and a comparison with donepezil

After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.

The therapeutic effect of Jiawei Danshen Decoction on myocardial ischemia-reperfusion injury by inhibiting H_(2)S-mediated autophagy signaling pathway

Objective To investigate the protective effects of Jiawei Danshen Decoction(加味丹参饮,JWDSD)on myocardial ischemia-reperfusion injury(MIRI)via the regulation of serum Hydrogen sulfide(H2S)and cardiac Beclin1,light Chain 3 A/B(LC3 A/B),p62,and autophagy protein5(ATG5).Methods Seventy specific pathogen free(SPF)Sprague-Dawley(SD)rats were randomly assigned to seven groups(n=10 in each group),including normal control,sham operation,MIRI model(model),ischemic preconditioning,Na HS,JWDSD,and JWDSD+CSE inhibitor(JWDSD+PPG)groups,and orally administered the indicated drugs for 14 d.Two hours after the last administration,the left anterior decreased branch of the coronary artery of each rat in model,Na HS,JWDSD,and JWDSD+PPG groups was ligated for 30 min and subsequently reperfused for 90 min to establish the MIRI model,and the rats in the sham operation group were only exposed to the thorax after surgery without coronary ligation.Blood samples were collected to detect H2S levels using an enzyme-linked immunosorbent assay(ELISA).Heart tissues were harvested for histopathological and immunohistochemical examination and quantitative reverse transcription polymerase chain reaction analysis of Beclin1 and ATG5 m RNA expression and Western blot analysis of Beclin1,LC3 A/B,and p62 protein expression.Results(1)The serum H2S content in model group rats was significantly reduced(P<0.01),JWDSD significantly increased the serum H2S content of model group rats(P<0.01),and the CSE inhibitor(PPG)significantly reduced H2S levels in the JWDSD group rats(P<0.01).(2)Compared with the normal control group,the myocardial tissue necrosis and cell destruction occurred in the MIRI model group,and JWDSD could alleviate the myocardial tissue necrosis of model rats,but the ameliorative effect of JWDSD could be reversed by PPG.(3)Beclin1,LC3 A/B,and p62 expression levels in the heart tissues of the model group were significantly increased(P<0.001),whereas decreased by JWDSD(P<0.05,P<0.01,and P<0.001,respectively),and the inhibitory effects of JWDSD on Beclin1,LC3 A/B,and p62 expression were partially reversed by PPG(P<0.01,P<0.05,and P<0.01,respectively).(4)The expression levels of autophagy-related genes Beclin1 and ATG5 were significantly increased in the model group(P<0.001).JWDSD clearly downregulated the expression levels of Beclin1 and ATG5(P<0.05 and P<0.001,respectively),which were reversed by PPG(P<0.001).Conclusion Our experimental data show that JWDSD can exhibit an anti-MIRI role by increasing endogenous H2S generation,and downregulating the expression of Beclin1,LC3 A/B,p62 and ATG5,which are related to inhibiting autophagy signaling.

外源H_(2)S对番茄双断根嫁接砧木及嫁接苗生长的影响

为筛选出适宜番茄双断根嫁接砧木根系再生及嫁接苗生长的硫化氢(H_(2)S)浓度,以金钻为砧木,以粉都金冠王为接穗,研究不同浓度NaHS(0.1、0.2、0.3、0.4、0.5 mmol·L^(-1),H_(2)S供体)对双断根番茄砧木及嫁接苗生长、根系形态的影响。结果表明,0.2 mmol·L^(-1) NaHS处理的断根番茄砧木株高、茎粗、叶面积、单株鲜质量和干质量、再生根系活力均达到最大值,且显著提高了砧木内源H_(2)S含量及D-半胱氨酸脱巯基酶(DCD)活性;亦显著促进了双断根嫁接苗的生长及根系发育。

The change of HERS cell number and gene expression profile by occlusion during root development in rat molars

Occlusion is commenced by contact of a tooth with an opposing tooth and is the mechanical force working against the periodontal ligament (PDL). Our recent study indicated that occlusion regulated tooth root elongation occurs during root development in rat molars. Using a non-occlusal model established to directly examine the effects of the absence of occlusion in developing first molars of upper jaw, histological analysis was performed to count the number of HERS cells, with Microarray used to analyse gene expression profiles. HERS cell numbers in normal molars decreased significantly more than those in experimental molars. In microarray data, a total of 59 genes showed significant differences (fold change > 2.0). Expressions of 55 genes in the experimental molars, which included PLAP-1/asporin and periostin, were significantly decreased than those in normal molars. These data indicate that occlusion during root development leads to a decrease in the number of HERS cells, and that the aforementioned genes may play an essential role in normal root formation.

Primary extraskeletal Ewing’s sarcoma of the lumbar nerve root:A case report

BACKGROUND Ewing’s sarcoma(ES)is a highly aggressive bone malignancy.Extraskeletal ES(EES)originating in the spinal canal is extremely rare.Herein,we report on a rare case of EES with a primary lumbar spinal nerve root including the complete diagnosis and treatment.CASE SUMMARY A young female patient presented with a complaint of right lower limb pain for 1 mo.Magnetic resonance imaging(MRI)revealed an 11 mm×14 mm×31 mm mass in the lumbar epidural region extending at the fifth lumbar spine(L5)level toward the right L5 neural foramen.Our initial diagnosis was an epidural schwannoma.The patient underwent total laminectomy,tumor resection and pedicle screw internal fixation and the L5 root tumor was found to have been completely removed intraoperatively.Histopathological examination of the lesion showed a typical ES with a large number of small,round cells.Immunohistochemistry analysis indicated positive CD99 and S100.After surgery,the patient received chemotherapy and radiotherapy with a 1 year of follow-up and no recurrent tumors or new lesions were found upon spine MRI and positron emission tomography/computed tomography reexamination.CONCLUSION Clinically,ES outside the bone should be considered when nerve root tumors are encountered inside and outside the spinal canal and the diagnosis should be determined by pathological biopsy.After surgical resection,chemotherapy and radiotherapy should be performed.After treatment,active follow-up and regular review should be completed.

Localization of P2X_7 Receptor Immunoreactivity in the Dorsal Root Ganglia of Guinea Pig

The P2X7 receptor mRNA and proteins in guinea-pig dorsal root ganglia （DRG） were studied by using RT-PCR and immunohistochemistry. The co-localization of P2X7 receptor with four cytochemical markers, the neurofilament protein NF200, S100, substance P and isolectin t34 （IB4） binding glyco-conjugates, were also examined. It was found that P2X7 receptor immunoreactivity （P2X7 R-IR） was present mostly in large-and medium-sized DRG neurons （62%±9% and 36%±6% respectively in all P2X7 R-IR neurons）. All the P2X7 R-IR neurons were also NF200 and S100 immunopositive. However, in a small number of NF200 or S100 immunopositive neurons no P2XTR-IR was detectable. All the IB4-positive or substance P-immunopositive neurons had no P2X7 R-IR. These results demonstrate that P2X7 receptors are expressed in a large subpopulation of DRG neurons and they may play a role in the transduction of specific peripheral sensory signals.

不同根管冲洗针头对S形弯曲根管冲洗效果的影响

目的:比较临床常用的2种冲洗针头对S形弯曲根管冲洗效果的影响。方法:采用80个树脂模拟弯曲S形根管,随机分成两组:IrriFlex组(A组)和NaviTip FX组(B组),分别预备至25^(#)0.06锥度和30^(#)0.04锥度各40个。根据根管冲洗针头、冲洗位置和预备形态分为A1组(IrriFlex,最大冲洗深度,25^(#)0.06锥度)、B1组(NaviTip FX,最大冲洗深度,25^(#)0.06锥度)、A2组(IrriFlex,最大冲洗深度,30^(#)0.04锥度)、B2组(NaviTip FX,最大冲洗深度,30^(#)0.04锥度)、A3组(IrriFlex,距根尖孔4 mm,25^(#)0.06锥度)、B3组(NaviTip FX,距根尖孔4 mm,25^(#)0.06锥度)、A4组(IrriFlex,距根尖孔4 mm,30^(#)0.04锥度)、B4组(NaviTip FX,距根尖孔4 mm,30^(#)0.04锥度),每组10个样本。先将1 mL亚甲蓝染色剂匀速注入树脂根管内,每根管使用3 mL 3%NaClO溶液匀速冲洗。在针头进入根管但尚未注射亚甲蓝时和注射亚甲蓝并冲洗完成后,使用牙科显微镜分别拍照,测量针头到根尖孔的距离及根尖部残留亚甲蓝的长度。采用SPSS 25.0软件对数据进行统计学分析。结果:在最大冲洗深度进行冲洗时,IrriFlex组均较NaviTip FX组深入距离根尖孔更近的位置(P<0.001),根尖部残余亚甲蓝的长度均显著减短(P<0.001);IrriFlex组中,两种终末预备号数对冲洗效果无显著影响(P>0.05),而NaviTip FX组中,25^(#)0.06锥度较30^(#)0.04锥度残余亚甲蓝的长度稍短(P<0.05)。当距根尖孔4 mm进行冲洗时,IrriFlex组与NaviTip FX组残留亚甲蓝长度无显著差异(P>0.05),25^(#)0.06锥度亚组较30^(#)0.04锥度亚组残余亚甲蓝的长度稍短(P<0.05)。结论:S形弯曲根管中,IrriFlex较NaviTip FX更有利于深入根管内进行冲洗。在选择使用NaviTip FX时,可考虑适当加大根管锥度以达到更好的冲洗效果。

Root Analogous Solid Waste Management System (RA-MSW for Biocells)

Root Analogous Municipal Solid Waste System “RA-MSW” is a novel management system that manages waste, biogas, and leachate in bioreactor landfill. Different than conventional disposal at landfills, waste is penetrated by synthetic roots that serve dual mission for biogas collection and leachate recirculation. RA-MSW roots are permeable hydropho- bic polymer material that are formed on-place or prefabricated at factory from recyclable material available on the dumping area. Since the material is hydrophobic, it does not contain water in the voids providing more space for gas transport and collection. The roots are also interchangeably used for leachate recirculation where the parameters of wa- ter content, nutrients, pH, and temperature are optimized to enhance biogas production simultaneously with leachate treatment. RA-MSW provides new approach of waste management and control;it has new virtues of landfill operation;new material medium for biogas collection;new biocell design configuration;new manipulation for leachate and biogas. The approach adds new merits for solid waste management, bio-energy utilization, and the concept of 5 R’s cycle for MSW management.

Effects of Raspberry Root decoction on cytokine level and thymus and spleen index in S180 model mice

Objective: To observe the effects of RRD on serum levels of cytokines interleukin-2 (IL-2), interleukin-10 (IL-10) and thymus and spleen index in S180 mice, and to explore the mechanism of tumor inhibition by RRD. Methods: Fifty Kunming healthy mice, half male and half female, were randomly divided into five groups: normal control group, model control group, cyclophosphamide group (CTX group), red raspberry group (RRD group) and combined administration of red raspberry and cyclophosphamide group (RRD + CTX group), with 10 mice in each group only. The other 40 mice were injected with 0.2 mLS180 tumor suspension at the right axilla to make the model experiment, except 10 mice in the normal control group. The next day, the normal control group and model control group were given intragastric administration of 0.02 mL/g/d saline, CTX group was given intragastric administration of 0.4 mL/20 g/d saline and 20 mg/kg/d CTX, RRD group was given intragastric administration of 0.4 mL/20 g/d RRD, RRD+CTX group was given intragastric administration of 0.4 mL/20 g/d RRD and 20 mg/kg/d CTX for 10 d, once a day. Serum levels of IL-2 and IL-10 were measured by ELISA, and thymus and spleen indexes were measured. Results: Red raspberry rhizome decoction could increase serum IL-2 level (P < 0.05), decrease IL-10 level (P < 0.05), increase thymus index (P < 0.05) and decrease spleen index (P < 0.05) in S180 mice. Conclusion:The anti-tumor effect of the water decoction of red raspberry rhizome may be related to the regulation of immune suppression and the improvement of immune organ function of the tumor-bearing organism.

Aortic Root and Ascending Aortic Aneurysm Related to One Case. Reimplantation of the Right Coronary Artery by 8 mm Dacron Tube (Cabrol Hemi Mustache) and Review of the Literature

The aortic aneurysm is the 13th leading cause of death in Western countries. The incidence of thoracic aortic aneurysms is estimated at 4.5 cases per 100,000. The diagnosis is often made on a chest x-ray or other imaging tests, such as an echocardiogram done for other heart diseases. Echocardiography is the first test to assess the diameter of the ascending aorta and its progression over time. Most patients are first assessed and followed up with spiral thoracic computed tomography with injection of contrast medium, supplemented by 3-dimensional reconstruction of the aneurysm in order to improve the accuracy of measurements, identification of its proximal part and distal. When dilation of the ascending aorta reaches the critical diameter of 50 mm, there is a risk of aortic dissection or rupture. Supravalvular aneurysms are treated by replacing the ectatic portion with a Dacron® tube in the supracoronary position. Aortic root aneurysms, including coronary ostia, require tube replacement, reimplantation of coronary ostia, as well as surgery on the aortic valve. In this article, we report a case of aneurysm of the aortic root and the ascending aorta treated by aortic valve replacement and the ascending aorta associated with the Cabrol hemi-mustache technique and we review the literature.

S变换在不稳定滑坡体变形监测中的应用

为了对不稳定滑坡GNSS(Global Navigation Satellite System)监测数据进行有效处理,应提取GNSS监测数据中的有用信息,从而准确掌握滑坡体的变形特征及趋势,本文将S变换引入滑坡体GNSS监测数据去噪中。首先,为了检验S变换的去噪性能,将含噪声仿真信号作为实验数据,使用S变换对实验数据进行去噪;其次,将S变换应用于滑坡体实测GNSS监测数据去噪中,结果表明,S变换能够将含噪声模拟信号及实测GNSS监测数据中的噪声有效剔除,提取真实的有用信息,将S变换去噪结果与小波变换去噪结果对比得出S变换去噪后的均方根误差RMSE、信噪比SNR均优于小波变换,验证了S变换去噪的有效性及优越性。

数控系统中S型曲线加减速控制算法研究

为了解决数控系统中S型加减速算法解析式方法缺乏的问题,提出一种7段式S型曲线加减速控制算法。该算法将所有7段式S型曲线可能出现的情况归纳为11个一元多次方程,再利用求根公式得到加减速时间。自主研发一种多轴运动控制卡,验证了7段式S型曲线加减速控制算法的所有情况。验证结果表明:该算法简单有效、运行稳定,能够满足高速高精度数控要求。

欠发达地区县级公共图书馆法治建设任重道远——对鄂、渝、湘、黔若干县级图书馆的调研分析

文章选取了我国欠发达地区25个县级公共图书馆,围绕《中华人民共和国公共图书馆法》的具体实施情况开展深入调查。发现当前我国欠发达地区县级公共图书馆的法治化治理水平有所提升,但地方政府依法保障的情况仍然堪忧。为此提出了4个基层公共图书馆法治建设着力点,为新时代基层公共图书馆的法治化建设和高质量发展提供参考。