Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of ...Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of Cangfu Daotan pills were searched in the database of traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).GeneCards database and OMIM database were used to search for disease targets of PCOS;the intersection of the target of Cangfu Daotan pill and the disease target of PCOS was drawn by using R to obtain the intersection gene;protein interaction network was constructed through String online database;Cytoscape(3.2.1)was used to construct a‘major component-disease-target’network diagram and analyze the important nodes therein;KEGG and GO enrichment analyses were carried out on the obtained intersection genes through org data packets in R.Results:Network pharmacology analysis showed that 151 active ingredients involved in pathways were screened out,140 of which acted on PCOS,and 105 potential targets were used to exert therapeutic effects.After enrichment,1900 GO biological signals and 143 KEGG signals were obtained,and the first 14 drug targets were screened out.Conclusion:Cangfu Daotan pill may mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation and other signals through regulating key targets of JUN,MAPK3,HSP90AA1,MAPK1,ESR1,NR3C1,APP,MAPK14,MAPK8,VEGFA,EGFR,RB1,AR,IL6,mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation,etc.,and regulate biological processes such as drug reaction,nutrition and lipid metabolism,active oxygen,sugar metabolism,steroid hormone,etc.to achieve the effect of treating polycystic ovary syndrome.展开更多
基金Shanghai“Xinglin New Star”project[ZY(2018-2020)-RCPY-3012]Clinical study on improving the endometrial receptivity of patients with polycystic ovary syndrome by the method of invigorating the kidney,removing phlegm and promoting blood circulation(Project within budget of Shanghai university of traditional Chinese medicine)+1 种基金The construction project of Shanghai famous old traditional Chinese medicine experience research studio(No.SHGZS-2017026)Shanghai university of traditional Chinese medicine affiliated Shuguang hospital Siming fund(In-hospital project)
文摘Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of Cangfu Daotan pills were searched in the database of traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).GeneCards database and OMIM database were used to search for disease targets of PCOS;the intersection of the target of Cangfu Daotan pill and the disease target of PCOS was drawn by using R to obtain the intersection gene;protein interaction network was constructed through String online database;Cytoscape(3.2.1)was used to construct a‘major component-disease-target’network diagram and analyze the important nodes therein;KEGG and GO enrichment analyses were carried out on the obtained intersection genes through org data packets in R.Results:Network pharmacology analysis showed that 151 active ingredients involved in pathways were screened out,140 of which acted on PCOS,and 105 potential targets were used to exert therapeutic effects.After enrichment,1900 GO biological signals and 143 KEGG signals were obtained,and the first 14 drug targets were screened out.Conclusion:Cangfu Daotan pill may mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation and other signals through regulating key targets of JUN,MAPK3,HSP90AA1,MAPK1,ESR1,NR3C1,APP,MAPK14,MAPK8,VEGFA,EGFR,RB1,AR,IL6,mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation,etc.,and regulate biological processes such as drug reaction,nutrition and lipid metabolism,active oxygen,sugar metabolism,steroid hormone,etc.to achieve the effect of treating polycystic ovary syndrome.
文摘目的 基于“百病皆为痰作祟”理论探讨祛风导痰汤对续发性全身强直-阵挛性发作癫痫患者的临床疗效。方法 选取2018年1月—2020年3月续发性全身强直-阵挛性发作癫痫患者92例,随机分两组,对照组采用电子生物反馈技术;联合组采用基于“百病皆为痰作祟”理论的祛风导痰汤联合电子生物反馈技术,持续3个月。治疗后评价患者临床疗效、癫痫生活质量评定量表(quality of life rating scale for patients with epilepsy, QOLIE-31)、连线测验A-B、数字符号转换测验(digital symbol conversion test, DSST)、词语流畅性测验(verbal fluency test, VFT)、数字广度测验(digit span test, DST)等,比较两组不良反应。结果 对照组的有效率78.26%(36/46)低于联合组93.48%(43/46),有统计学意义(P<0.05)。对照组治疗3个月综合质量、情绪、总体健康、药物影响、认知功能、社会功能、精力、对发作担忧评分均低于联合组,有统计学意义(P<0.05)。对照组治疗3个月连线测验A、连线测验B高于联合组,DSST评分、VFT评分、DST评分低于联合组,有统计学意义(P<0.05)。对照组总不良反应率23.91%(11/46)与联合组28.26%(13/46)比较,无统计学意义(P>0.05)。结论 基于“百病皆为痰作祟”理论的祛风导痰汤对续发性全身强直-阵挛性发作癫痫患者的临床疗效显著,可以改善认知功能,从而提高生活质量。
