A survey of 35 tree species （belonging to 28 genera in 19 families） in Aliyar, South India was carried out to ascertain their arbuscular mycorrhizal （AM） and dark septate endophyte （DSE） fungal status. All the t...A survey of 35 tree species （belonging to 28 genera in 19 families） in Aliyar, South India was carried out to ascertain their arbuscular mycorrhizal （AM） and dark septate endophyte （DSE） fungal status. All the tree species examined had AM association. AM and DSE coloni- zation is reported for the first time in 20 and 14 species respectively. Co- occurrence of AM and DSE was observed in 14 （40%） tree species. The extent of DSE colonization was inversely related to the extent of AM fungal colonization. Six tree species had Arum-type, 18 had intermediate- type and 11 had typical Paris-type AM morphology. AM fungal spore morphotypes belonging to 11 species in two genera were isolated from the rhizosphere soil. AM fungal spore numbers were not related to the ex- tent of AM colonization and Glomus dominated spore diversity. AM association individually and along with DSE were found respectively in the 63% and 44% of the economically important tree species. The occur- rence of AM and DSE fungal association in economically important indigenous tree species indicates the possibility of exploiting this asso- ciation in future conservation programmes of these species.展开更多
Sweet cherry is one of the most popular fresh fruits in the world.Previously,we isolated a soilborne dark septate endophyte(DSE)strain,S16,which promoted the growth of Gisela 5 sweet cherry rootstock.However,little is...Sweet cherry is one of the most popular fresh fruits in the world.Previously,we isolated a soilborne dark septate endophyte(DSE)strain,S16,which promoted the growth of Gisela 5 sweet cherry rootstock.However,little is known about the molecular mechanism of the effect of S16 on the growth of sweet cherry.In this study,the physiological parameters and transcript profiles of sweet cherry roots were analyzed under S16 treatment compared with a control to elucidate the molecular mechanisms of the effect of this strain on sweet cherry growth.After inoculation with S16,sweet cherry seedlings exhibited more vigorous growth.Moreover,we identified 4249 differentially expressed genes(DEGs)between S16-treated plants and the control.Many of the DEGs are involved in pathways related to plant growth,such as cellular metabolic and plant hormone pathways.Additionally,some genes involved in nitrate regulation were also enriched;and these genes may be involved in the regulation of nitrate uptake in plants.Physiological index detection demonstrated that S16 could improve the nitrate assimilation of sweet cherry via NO3–transporters.This RNA-seq dataset provides comprehensive insight into the transcriptomic landscape to reveal the molecular mechanisms whereby the DSE influences the growth of sweet cherry.展开更多
通过平皿和盆栽共生对抗法,筛选到1株对香蕉枯萎病具有防治作用的深色有隔内生真菌L-14,2种方法的防效分别达到72.4%和56.5%,接种该菌株可显著提高香蕉幼苗的鲜重。形态观察和28S r DNA序列比对分析结果表明,该菌株为裂壳菌(Schizotheci...通过平皿和盆栽共生对抗法,筛选到1株对香蕉枯萎病具有防治作用的深色有隔内生真菌L-14,2种方法的防效分别达到72.4%和56.5%,接种该菌株可显著提高香蕉幼苗的鲜重。形态观察和28S r DNA序列比对分析结果表明,该菌株为裂壳菌(Schizothecium sp.)。使用该菌株浸根处理接种香蕉苗后,植株系列抗氧化保护酶如苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)及超氧化物歧化酶(SOD)的活性均显著高于对照,而菌株L-14与香蕉枯萎病菌混合接种处理样品的PPO和SOD活性显著高于单独接种处理,表明接种该生防菌能增强香蕉的抗氧化防护系统,提高其对香蕉枯萎病的抗性。展开更多
文摘A survey of 35 tree species （belonging to 28 genera in 19 families） in Aliyar, South India was carried out to ascertain their arbuscular mycorrhizal （AM） and dark septate endophyte （DSE） fungal status. All the tree species examined had AM association. AM and DSE coloni- zation is reported for the first time in 20 and 14 species respectively. Co- occurrence of AM and DSE was observed in 14 （40%） tree species. The extent of DSE colonization was inversely related to the extent of AM fungal colonization. Six tree species had Arum-type, 18 had intermediate- type and 11 had typical Paris-type AM morphology. AM fungal spore morphotypes belonging to 11 species in two genera were isolated from the rhizosphere soil. AM fungal spore numbers were not related to the ex- tent of AM colonization and Glomus dominated spore diversity. AM association individually and along with DSE were found respectively in the 63% and 44% of the economically important tree species. The occur- rence of AM and DSE fungal association in economically important indigenous tree species indicates the possibility of exploiting this asso- ciation in future conservation programmes of these species.
基金supported by the National Natural Science Foundation of China(31570649)the Key Research and Development Program of Yantai City,Shandong Province,China(2019XDHZ089)+1 种基金the Natural Science Foundation of Shandong Province,China(ZR2019PC052 and 2017C03)the Key Research and Development Program of Shandong Province,China(2019GSF107091,2019GSF109114,2018YYSP004 and 2018GNC110028)。
文摘Sweet cherry is one of the most popular fresh fruits in the world.Previously,we isolated a soilborne dark septate endophyte(DSE)strain,S16,which promoted the growth of Gisela 5 sweet cherry rootstock.However,little is known about the molecular mechanism of the effect of S16 on the growth of sweet cherry.In this study,the physiological parameters and transcript profiles of sweet cherry roots were analyzed under S16 treatment compared with a control to elucidate the molecular mechanisms of the effect of this strain on sweet cherry growth.After inoculation with S16,sweet cherry seedlings exhibited more vigorous growth.Moreover,we identified 4249 differentially expressed genes(DEGs)between S16-treated plants and the control.Many of the DEGs are involved in pathways related to plant growth,such as cellular metabolic and plant hormone pathways.Additionally,some genes involved in nitrate regulation were also enriched;and these genes may be involved in the regulation of nitrate uptake in plants.Physiological index detection demonstrated that S16 could improve the nitrate assimilation of sweet cherry via NO3–transporters.This RNA-seq dataset provides comprehensive insight into the transcriptomic landscape to reveal the molecular mechanisms whereby the DSE influences the growth of sweet cherry.
文摘通过平皿和盆栽共生对抗法,筛选到1株对香蕉枯萎病具有防治作用的深色有隔内生真菌L-14,2种方法的防效分别达到72.4%和56.5%,接种该菌株可显著提高香蕉幼苗的鲜重。形态观察和28S r DNA序列比对分析结果表明,该菌株为裂壳菌(Schizothecium sp.)。使用该菌株浸根处理接种香蕉苗后,植株系列抗氧化保护酶如苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)及超氧化物歧化酶(SOD)的活性均显著高于对照,而菌株L-14与香蕉枯萎病菌混合接种处理样品的PPO和SOD活性显著高于单独接种处理,表明接种该生防菌能增强香蕉的抗氧化防护系统,提高其对香蕉枯萎病的抗性。
