One specimen was collected from the main course of Lancangjiang River （upper reach of Mekong） in Guanlei Port, Xishuangbanna, Yunnan, China in April, 2008. It is identified as Dasyatis laosensis, a new record of Das...One specimen was collected from the main course of Lancangjiang River （upper reach of Mekong） in Guanlei Port, Xishuangbanna, Yunnan, China in April, 2008. It is identified as Dasyatis laosensis, a new record of Dasyatidae species in China. It could be distinguished from other Dasyatis species by a combination of the following characteristics： one enlarged venomous spine on the tail, ventral surface of body with orange marginal coloration, tail length greater than body length.展开更多
AIM:Dasyatis jenkinsii is used traditionally to treat inflammatory complaints and arthritis by the fisherman community and local population.The present study was designed to scientifically investigate the traditional ...AIM:Dasyatis jenkinsii is used traditionally to treat inflammatory complaints and arthritis by the fisherman community and local population.The present study was designed to scientifically investigate the traditional practice through the analgesic,anti-inflammatory,and organ toxicity studies and characterization of bioactive compounds of crude extracts of D.jenkinsii.METHOD: Solvent extract of homogenized fresh fish was prepared using petroleum ether and diethyl ether.The chemical and spectral analyses of extracts were carried out using FT-IR and GC-MS.Analgesic and anti-inflammatory activities were assessed by hot plate,tail clip,and carrageenan induced rat paw edema methods.The organ toxicity of each extract was assessed on brain,liver,and kidney of mice.RESULTS:The IR spectrum indicated the presence of aromatic and aliphatic compounds.GC-MS analysis revealed the presence of 1-(4-carboxy)phenylnona-2,5-diene and 3-hydroxymono-glyceryl hydrogen phthalate in the petroleum ether extract and carboxyl serine,dihydrotryptophan,and indolyl carboxylic acid in the diethyl ether extract.Both extracts showed significant analgesic and anti-inflammatory effects in all the methods tested.The vital organs of the test animals were not affected by the crude extracts significantly.CONCLUSIONS:The presence of biologically active compounds in the crude extracts with analgesic and anti-inflammatory properties justifies the traditional knowledge and paves the way for isolation of these compounds for further experimentation.展开更多
基金This study was supported by the National Science Foundation of China (30970326, U0936602, 30870291)the Scientific Research Foundation of Yunnan University (2008YB004)
文摘One specimen was collected from the main course of Lancangjiang River （upper reach of Mekong） in Guanlei Port, Xishuangbanna, Yunnan, China in April, 2008. It is identified as Dasyatis laosensis, a new record of Dasyatidae species in China. It could be distinguished from other Dasyatis species by a combination of the following characteristics： one enlarged venomous spine on the tail, ventral surface of body with orange marginal coloration, tail length greater than body length.
文摘AIM:Dasyatis jenkinsii is used traditionally to treat inflammatory complaints and arthritis by the fisherman community and local population.The present study was designed to scientifically investigate the traditional practice through the analgesic,anti-inflammatory,and organ toxicity studies and characterization of bioactive compounds of crude extracts of D.jenkinsii.METHOD: Solvent extract of homogenized fresh fish was prepared using petroleum ether and diethyl ether.The chemical and spectral analyses of extracts were carried out using FT-IR and GC-MS.Analgesic and anti-inflammatory activities were assessed by hot plate,tail clip,and carrageenan induced rat paw edema methods.The organ toxicity of each extract was assessed on brain,liver,and kidney of mice.RESULTS:The IR spectrum indicated the presence of aromatic and aliphatic compounds.GC-MS analysis revealed the presence of 1-(4-carboxy)phenylnona-2,5-diene and 3-hydroxymono-glyceryl hydrogen phthalate in the petroleum ether extract and carboxyl serine,dihydrotryptophan,and indolyl carboxylic acid in the diethyl ether extract.Both extracts showed significant analgesic and anti-inflammatory effects in all the methods tested.The vital organs of the test animals were not affected by the crude extracts significantly.CONCLUSIONS:The presence of biologically active compounds in the crude extracts with analgesic and anti-inflammatory properties justifies the traditional knowledge and paves the way for isolation of these compounds for further experimentation.
