The present article covers a simple approach to detect and subsequently identify in vivo metabolites of brodimoprim, using high performance liquid chromatography coupled to ion trap mass spectrometer（LC/ESI-MS）, whi...The present article covers a simple approach to detect and subsequently identify in vivo metabolites of brodimoprim, using high performance liquid chromatography coupled to ion trap mass spectrometer（LC/ESI-MS）, which is based on a data-dependent acquisition of isotope ions and result verified by full scan mass spectrum. The distinguished advantage of data-dependent scan is rapidness because it requires minimum sample preparation, and all the necessary data can be obtained in one chromatographic run. In addition, it is highly sensitive and selective, allowing detection of trace metabolites even in the presence of complex biomatrix. As a result, four phase-Ⅰ（M1--M4） and four Phase-Ⅱ（M5--M8） metabolites of brodimoprim were identified in urine after the oral administration of hrodimoprim to Wistar rats. Their chemical structures were proposed based on the interpretation of their CID fragmentation characterizations and the metabolic pathway was exhibited in this article.展开更多
【目的】林木参数是森林蓄积量、森林生物量估算的基础指标,传统的人工调查方式费时费力,已难以适应新形势下数字化森林资源监测技术的要求。地面激光雷达扫描技术能够获取小尺度高分辨率的林分内部结构信息,为林分环境条件下林木胸径...【目的】林木参数是森林蓄积量、森林生物量估算的基础指标,传统的人工调查方式费时费力,已难以适应新形势下数字化森林资源监测技术的要求。地面激光雷达扫描技术能够获取小尺度高分辨率的林分内部结构信息,为林分环境条件下林木胸径、树高提取提供一种新的思路。【方法】以芦头实验林场杉木林样地为研究对象,针对FARO Focus 3D X330三维激光扫描仪设计了7种不同的扫描组合方式对样地进行扫描,提出象限角点云简化思路进行参数提取和精度评价,探究不同扫描组合方式对林木胸径、树高参数提取精度与效率的影响。【结果】1)当扫描分辨率为1/2、质量为4X时,胸径参数提取精度最高;当扫描分辨率为1/4、质量为4X时,树高参数提取精度最高。2)在林木参数提取结果没有显著性差异的前提下,扫描分辨率为1/4、质量为4X的扫描参数工作效率最高。3)选取同时兼顾精度和效率的1/4扫描分辨率、质量4X的扫描结果,进行象限角点云简化,简化的点云能够准确地提取出林木胸径参数。【结论】研究结果对于具有相同或相似地理条件和树种的林地选择扫描参数和点云简化方式具有重要参考价值,可以提高内业工作效率,同时也为地面激光雷达野外样地调查提供方法和技术参考。展开更多
基金the National Natural Science Foundation of China(Nos.30630075 and 20675056)Natural Science Founda-tion of Tianjin City,China(No.07JCYBJC01600)
文摘The present article covers a simple approach to detect and subsequently identify in vivo metabolites of brodimoprim, using high performance liquid chromatography coupled to ion trap mass spectrometer（LC/ESI-MS）, which is based on a data-dependent acquisition of isotope ions and result verified by full scan mass spectrum. The distinguished advantage of data-dependent scan is rapidness because it requires minimum sample preparation, and all the necessary data can be obtained in one chromatographic run. In addition, it is highly sensitive and selective, allowing detection of trace metabolites even in the presence of complex biomatrix. As a result, four phase-Ⅰ（M1--M4） and four Phase-Ⅱ（M5--M8） metabolites of brodimoprim were identified in urine after the oral administration of hrodimoprim to Wistar rats. Their chemical structures were proposed based on the interpretation of their CID fragmentation characterizations and the metabolic pathway was exhibited in this article.
文摘【目的】林木参数是森林蓄积量、森林生物量估算的基础指标,传统的人工调查方式费时费力,已难以适应新形势下数字化森林资源监测技术的要求。地面激光雷达扫描技术能够获取小尺度高分辨率的林分内部结构信息,为林分环境条件下林木胸径、树高提取提供一种新的思路。【方法】以芦头实验林场杉木林样地为研究对象,针对FARO Focus 3D X330三维激光扫描仪设计了7种不同的扫描组合方式对样地进行扫描,提出象限角点云简化思路进行参数提取和精度评价,探究不同扫描组合方式对林木胸径、树高参数提取精度与效率的影响。【结果】1)当扫描分辨率为1/2、质量为4X时,胸径参数提取精度最高;当扫描分辨率为1/4、质量为4X时,树高参数提取精度最高。2)在林木参数提取结果没有显著性差异的前提下,扫描分辨率为1/4、质量为4X的扫描参数工作效率最高。3)选取同时兼顾精度和效率的1/4扫描分辨率、质量4X的扫描结果,进行象限角点云简化,简化的点云能够准确地提取出林木胸径参数。【结论】研究结果对于具有相同或相似地理条件和树种的林地选择扫描参数和点云简化方式具有重要参考价值,可以提高内业工作效率,同时也为地面激光雷达野外样地调查提供方法和技术参考。
