Objective： The Taiwan Residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components ...Objective： The Taiwan Residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components alter bone metabolism. Methods： The effects of low and high doses of D. formosana water extracts （DFW, 50 and 200 mg/（kg·d）） and （-）-epicatechin-3-O-D-allopyranoside isolates of DFW （ECAP, 0.06 and 2.5 mg/（kg·d）） on protein lev- els of important indicators of osteoclast and osteoblast activities were examined. Ovariectomized mice were used as a model of osteoporosis. Bone morphogenetic protein 2 （BMP-2）, collagen 1 （COL-1）, alka- line phosphatase （ALP）, runt-related transcription factor 2 （RUNX2）, osteocalcin （OCN）, and osteopontin （OPN） were analyzed using western blotting of femur tissue. Interleukin （IL）-1β and tumor necrosis fac- tor （TNF）-a were measured in the serum, and hematoxylin and eosin staining were used to assess bone morphology. Results： Treatment with DFW or ECAP significantly induced BMP-2, COL-l, ALP, RUNX2, OCN, and OPN protein expression, indicating stimulation of osteogenesis. The treatments also reduced IL-1β and TNF- expression, indicating inhibition of osteoclastogenesis. In histological examinations, mice treated with DFW or ECAP had more bone trabeculae. The results demonstrate that DFW and ECAP inhibit osteo- clast differentiation and promote osteoblast differentiation, and effectively ameliorate ovariectomy （OVX）- induced osteoporosis. Conclusion： ECAP is a major component of DFW, which may have therapeutic potential for the treatment of diseases associated with excessive osteoclastic and insufficient osteogenic activity. ECAP may not be the specific active molecule because it is unstable in the stomach. However, the major active molecule needs to be confirmed using a relevant pharmacological model.展开更多
采用走访和实地调查相结合的方法对广西优势药材骨碎补(Davallia mariesii Moore ex Bak.)种质资源种类与分布、生物生态学特性、资源及开发利用状况、有效成分含量等进行调查研究。结果表明,广西骨碎补种质资源丰富,基源植物共3科5属7...采用走访和实地调查相结合的方法对广西优势药材骨碎补(Davallia mariesii Moore ex Bak.)种质资源种类与分布、生物生态学特性、资源及开发利用状况、有效成分含量等进行调查研究。结果表明,广西骨碎补种质资源丰富,基源植物共3科5属7种。骨碎补原植物喜温暖湿润的气候环境,多附生于林中岩石或树干上,偶生于墙上,土生较少。广西骨碎补药材品质佳,质量好,有较好的利用和开发价值。骨碎补生境受到一定程度破坏,资源逐渐减少,建议加强对骨碎补种质资源收集和原生境保护,开展种苗繁育和人工栽培技术研究,建立较大规模的人工种植与繁育基地,为资源可持续利用提供保障。展开更多
基金Yupintang Traditional Chinese Medicine Foundation for continuously supporting me through my research
文摘Objective： The Taiwan Residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components alter bone metabolism. Methods： The effects of low and high doses of D. formosana water extracts （DFW, 50 and 200 mg/（kg·d）） and （-）-epicatechin-3-O-D-allopyranoside isolates of DFW （ECAP, 0.06 and 2.5 mg/（kg·d）） on protein lev- els of important indicators of osteoclast and osteoblast activities were examined. Ovariectomized mice were used as a model of osteoporosis. Bone morphogenetic protein 2 （BMP-2）, collagen 1 （COL-1）, alka- line phosphatase （ALP）, runt-related transcription factor 2 （RUNX2）, osteocalcin （OCN）, and osteopontin （OPN） were analyzed using western blotting of femur tissue. Interleukin （IL）-1β and tumor necrosis fac- tor （TNF）-a were measured in the serum, and hematoxylin and eosin staining were used to assess bone morphology. Results： Treatment with DFW or ECAP significantly induced BMP-2, COL-l, ALP, RUNX2, OCN, and OPN protein expression, indicating stimulation of osteogenesis. The treatments also reduced IL-1β and TNF- expression, indicating inhibition of osteoclastogenesis. In histological examinations, mice treated with DFW or ECAP had more bone trabeculae. The results demonstrate that DFW and ECAP inhibit osteo- clast differentiation and promote osteoblast differentiation, and effectively ameliorate ovariectomy （OVX）- induced osteoporosis. Conclusion： ECAP is a major component of DFW, which may have therapeutic potential for the treatment of diseases associated with excessive osteoclastic and insufficient osteogenic activity. ECAP may not be the specific active molecule because it is unstable in the stomach. However, the major active molecule needs to be confirmed using a relevant pharmacological model.
文摘采用走访和实地调查相结合的方法对广西优势药材骨碎补(Davallia mariesii Moore ex Bak.)种质资源种类与分布、生物生态学特性、资源及开发利用状况、有效成分含量等进行调查研究。结果表明,广西骨碎补种质资源丰富,基源植物共3科5属7种。骨碎补原植物喜温暖湿润的气候环境,多附生于林中岩石或树干上,偶生于墙上,土生较少。广西骨碎补药材品质佳,质量好,有较好的利用和开发价值。骨碎补生境受到一定程度破坏,资源逐渐减少,建议加强对骨碎补种质资源收集和原生境保护,开展种苗繁育和人工栽培技术研究,建立较大规模的人工种植与繁育基地,为资源可持续利用提供保障。
