Objective:To study the aphrodisiac potential of Polyalthia(P.)bullata in fowl.Methods:In this study,testosterone,as an indicator of the aphrodisiac potential of P.bullata,was investigated for its release from TM3 Leyd...Objective:To study the aphrodisiac potential of Polyalthia(P.)bullata in fowl.Methods:In this study,testosterone,as an indicator of the aphrodisiac potential of P.bullata,was investigated for its release from TM3 Leydig cells grown in vitro and in 4 fowls given capsules containing P.bullata at a dose of 10 mg in each capsule twice a day,for 50 days.In the latter in vivo evaluation,mating behaviours were additionally determined after the treated fowls were released to the individual hens,and their testes and liver were dissected for histological examinations.Blood drawn from the fowls was assessed for any changes in diagnostic parameters.Results:In the in vitro test(TM3 Leydig cells),P.bullata was able to increase testosterone to 0.48 nmol/L within 72 h of incubation,compared to the untreated control with only 0.18 nmol/L,i.e.,an increase of 170%.In the in vivo test,outcomes in the fowls dosed with P.bullata showed similar positive elevations of testosterone to(9.72±1.10)nmol/L in comparison to the controls that showed a level of only(4.05±0.84)nmol/L.Total frequencies of mating behaviours were observed(wing flapping,body shakes,crowing and beak pecking)to be 23 counts for the test compared to only 15 for the control fowls.Histological examination of the male reproductive organs provided evidence of testosterone boosting based on an observable increase in the activity at the seminiferous tubules of testis tissues without any damaging effects,compared to the controls.In the nine diagnostic blood parameters assessed,including alanine aminotransferase,aspartate aminotransferase,and gamma glutamyltransferase,none was remarkably elevated compared to the controls.The histological changes in the liver were not severe and mainly consisted of only localized moderate but recoverable obstructions and swellings of the vessels and tubules.Conclusions:P.bullata is able to boost testosterone both in vitro and in vivo,with no acute toxicities.展开更多
采用走访和实地调查相结合的方法对广西优势药材骨碎补(Davallia mariesii Moore ex Bak.)种质资源种类与分布、生物生态学特性、资源及开发利用状况、有效成分含量等进行调查研究。结果表明,广西骨碎补种质资源丰富,基源植物共3科5属7...采用走访和实地调查相结合的方法对广西优势药材骨碎补(Davallia mariesii Moore ex Bak.)种质资源种类与分布、生物生态学特性、资源及开发利用状况、有效成分含量等进行调查研究。结果表明,广西骨碎补种质资源丰富,基源植物共3科5属7种。骨碎补原植物喜温暖湿润的气候环境,多附生于林中岩石或树干上,偶生于墙上,土生较少。广西骨碎补药材品质佳,质量好,有较好的利用和开发价值。骨碎补生境受到一定程度破坏,资源逐渐减少,建议加强对骨碎补种质资源收集和原生境保护,开展种苗繁育和人工栽培技术研究,建立较大规模的人工种植与繁育基地,为资源可持续利用提供保障。展开更多
基金funded by grants awarded by Malaysian Technological University Network(MTUN)with grant number of UIC191201grants awarded by Universiti Malaysia Pahang with grant numbers of PDU203209(product development grant)PGRS1903203(postgraduate grant).
文摘Objective:To study the aphrodisiac potential of Polyalthia(P.)bullata in fowl.Methods:In this study,testosterone,as an indicator of the aphrodisiac potential of P.bullata,was investigated for its release from TM3 Leydig cells grown in vitro and in 4 fowls given capsules containing P.bullata at a dose of 10 mg in each capsule twice a day,for 50 days.In the latter in vivo evaluation,mating behaviours were additionally determined after the treated fowls were released to the individual hens,and their testes and liver were dissected for histological examinations.Blood drawn from the fowls was assessed for any changes in diagnostic parameters.Results:In the in vitro test(TM3 Leydig cells),P.bullata was able to increase testosterone to 0.48 nmol/L within 72 h of incubation,compared to the untreated control with only 0.18 nmol/L,i.e.,an increase of 170%.In the in vivo test,outcomes in the fowls dosed with P.bullata showed similar positive elevations of testosterone to(9.72±1.10)nmol/L in comparison to the controls that showed a level of only(4.05±0.84)nmol/L.Total frequencies of mating behaviours were observed(wing flapping,body shakes,crowing and beak pecking)to be 23 counts for the test compared to only 15 for the control fowls.Histological examination of the male reproductive organs provided evidence of testosterone boosting based on an observable increase in the activity at the seminiferous tubules of testis tissues without any damaging effects,compared to the controls.In the nine diagnostic blood parameters assessed,including alanine aminotransferase,aspartate aminotransferase,and gamma glutamyltransferase,none was remarkably elevated compared to the controls.The histological changes in the liver were not severe and mainly consisted of only localized moderate but recoverable obstructions and swellings of the vessels and tubules.Conclusions:P.bullata is able to boost testosterone both in vitro and in vivo,with no acute toxicities.
文摘采用走访和实地调查相结合的方法对广西优势药材骨碎补(Davallia mariesii Moore ex Bak.)种质资源种类与分布、生物生态学特性、资源及开发利用状况、有效成分含量等进行调查研究。结果表明,广西骨碎补种质资源丰富,基源植物共3科5属7种。骨碎补原植物喜温暖湿润的气候环境,多附生于林中岩石或树干上,偶生于墙上,土生较少。广西骨碎补药材品质佳,质量好,有较好的利用和开发价值。骨碎补生境受到一定程度破坏,资源逐渐减少,建议加强对骨碎补种质资源收集和原生境保护,开展种苗繁育和人工栽培技术研究,建立较大规模的人工种植与繁育基地,为资源可持续利用提供保障。
