Situated in the Shilong Industrial District of Mentougou, Beijing, the Beijing Dayuan Non-woven Fabric Co. Ltd is a Sino-foreign joint venture producing non-woven fabrics, set up in 1991.
Colorectal mucinous adenocarcinoma has the characteristics of high malignant degree,high mortality and low survival rate,easy to recurrence and metastasis after operation,and the prognosis of survival patients is also...Colorectal mucinous adenocarcinoma has the characteristics of high malignant degree,high mortality and low survival rate,easy to recurrence and metastasis after operation,and the prognosis of survival patients is also very poor.The research on immune-related therapy and tumor microenvironment has become a research hotspot.From the point of view of traditional Chinese medicine,Chaihu Dayuan Yin can dispel dampness,resolve phlegm and regulate Sanjiao membrane.It is suitable for tumors with phlegm-dampness and qi stagnation,especially those with abdominal and pelvic dampness heavier than heat,such as colorectal mucinous adenocarcinoma and so on.The concept of resolving phlegm and dredging Sanjiao based on the addition and subtraction of Chaihu Dayuan Yin is of great value to the clinical treatment and basic scientific research of tumors.展开更多
OBJECTIVE:To study the anti-inflammatory and anti-tussive effects of Qingfei Dayuan granules(清肺达原颗粒,QFDY),and to evaluate the acute and sub-chronic toxicity of QFDY.METHODS:Anti-inflammatory effects were evaluat...OBJECTIVE:To study the anti-inflammatory and anti-tussive effects of Qingfei Dayuan granules(清肺达原颗粒,QFDY),and to evaluate the acute and sub-chronic toxicity of QFDY.METHODS:Anti-inflammatory effects were evaluated by murine model of xylene induced ear edema in mice.Ear swelling degree was calculated and tumor necrosis factor-α,interleukin-1βand interleukin-6 were determined.Anti-tussive evaluations were carried out in the mouse cough model induced by ammonia liquor.Latent period cough and number of cough within 3 min were counted.In acute toxicity study,the rats were randomly divided into test group and solvent control group.Body weighs,food intakes and general clinical signs were monitored.In the sub-chronic toxicity study,QFDY was administered to rats at 0,4,8 and 16 g/kg per day for 28 and 30 d of post treatment was conducted.Mortalities,clinical signs,body weight changes,food intakes,ophthalmological examinations,hematological parameters,biochemical indicators,electrolyte indicators,urinalyses and histopathological examinations were monitored.RESULTS:QFDY significantly inhibited the development of ear edema in anti-inflammatory assay and decreased cough frequency caused by ammonia liquor.The results presented a dose-effect relationship.In acute toxicity study,no abnormality exhibited at dose of 24.0 g/kg per day during the 14-d observation period.In the sub-chronic toxicity study,higher reticulocyte count,lymphocyte and lower Cl-,blood urea nitrogen were analyzed compared with the solvent control group.But the differences were considered to be incidental and not clinically toxic.Obvious dose-effect relationship of urine color was observed,and the three test groups at the end of the experiments resulted in significant increase in urobilinogen,bilirubin,ketone body and urine leukocyte.However,all the positive indicators returned to normal in the recovery period.Therefore,no toxicological changes were found during the study period.CONCLUSION:QFDY showed significant anti-inflammatory and anti-tussive effects in mice.The lethal dose(LD50)of per oral QFDY in rats was estimated to be more than 24.0 g/kg per day and the no observed adverse effect level was over 16 g/kg per day,which suggested that QFDY is relatively safe for oral medication at the present dose on rats.Our experimental results provide a reference for the further development and research of QFDY.展开更多
OBJECTIVE:To observe the effects of New Dayuan powder(NDYP)on Methicillin-resistant Staphylococcus aureus(MRSA)biofilms and the embedded bacteria in vitro.METHODS:2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazol...OBJECTIVE:To observe the effects of New Dayuan powder(NDYP)on Methicillin-resistant Staphylococcus aureus(MRSA)biofilms and the embedded bacteria in vitro.METHODS:2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide(XTT)assays were used to study the effects of NDYP on developing MRSA biofilms:100μL of bacterial culture and100μL drug solution were added to wells of96-well plates.After 24 h of incubation,the plates were washed and XTT-phenazine methyl sulfate(PMS)was added to enable counting of the number of live bacteria in biofilms using a microplate reader.XTT assays were also used to explore the effects of NDYP on mature MRSA biofilms:100μL of bacterial culture were added to wells of 96-well plates.Bacteria were cultured in the plates for 24 h,and then drug solution was added.The plates were cultured for another 24 h,and then XTT-PMS was added to detect the number of live bacteria in the biofilms.Scanning electron microscopy(SEM)was used to observe the effects of NDYP on mature MRSA biofilms:washed and sterilized glass coverslips were added to 24-well plates.Bacterial culture was added.After 24 h of incubation,drug solution was added.After another 24 h of incubation,the samples were observed by SEM.RESULTS:XTT assays showed that the number of live bacteria in both developing and mature MRSA biofilms decreased significantly(P<0.01)after the administration of NDYP.SEM images showed that NDYP could destroy the structure of the bacteria and resulted in uneven thickness of MRSA biofilms.CONCLUSION:In vitro,NDYP has obvious inhibitory effects on the formation of MRSA biofilms and on mature biofilms.展开更多
目的运用网络药理学方法对达原饮进行分析,探索达原饮治疗发热的相关靶点与通路,明确其作用机制与潜在治疗疾病,为后续研究提供指导。方法通过中药系统药理学数据库与分析平台(TCMSP)检索达原饮各组成药物的主要化学成分及作用靶点,参...目的运用网络药理学方法对达原饮进行分析,探索达原饮治疗发热的相关靶点与通路,明确其作用机制与潜在治疗疾病,为后续研究提供指导。方法通过中药系统药理学数据库与分析平台(TCMSP)检索达原饮各组成药物的主要化学成分及作用靶点,参考类药五原则并结合口服生物利用度对成分靶点数据进行筛选,通过String数据库检索筛选后的靶点,得到达原饮作用靶点基因,在CTD数据库中检索发热(fever)相关作用靶点基因,对二者进行比较分析,取重复值,得到达原饮治疗发热相关靶点;在String数据库中检索达原饮治疗发热相关靶点,得到达原饮治疗发热相关通路;利用Cytoscape3.7.0软件对通路进行模块化分析,选择分值最高者,在WEB-based GEne SeT AnaLysis Toolkit中进行可视化分析。结果得到达原饮治疗发热相关靶点432个,相关通路75条,其中特异性疾病相关通路21条,非特异性疾病相关通路54条。结论达原饮治疗发热的作用机制涉及以代谢为中心的多种途径,可能通过多靶点复杂通路的共同干预起效。本研究就达原饮对于癌性发热的治疗补充了数据支持,并推测达原饮治疗神经退行性疾病具有可能性。展开更多
文摘Situated in the Shilong Industrial District of Mentougou, Beijing, the Beijing Dayuan Non-woven Fabric Co. Ltd is a Sino-foreign joint venture producing non-woven fabrics, set up in 1991.
文摘Colorectal mucinous adenocarcinoma has the characteristics of high malignant degree,high mortality and low survival rate,easy to recurrence and metastasis after operation,and the prognosis of survival patients is also very poor.The research on immune-related therapy and tumor microenvironment has become a research hotspot.From the point of view of traditional Chinese medicine,Chaihu Dayuan Yin can dispel dampness,resolve phlegm and regulate Sanjiao membrane.It is suitable for tumors with phlegm-dampness and qi stagnation,especially those with abdominal and pelvic dampness heavier than heat,such as colorectal mucinous adenocarcinoma and so on.The concept of resolving phlegm and dredging Sanjiao based on the addition and subtraction of Chaihu Dayuan Yin is of great value to the clinical treatment and basic scientific research of tumors.
基金Department of Science and Technology of Hubei Province:Preclinical Study on Qingfei Dayuan granules (No.2021BGE017)
文摘OBJECTIVE:To study the anti-inflammatory and anti-tussive effects of Qingfei Dayuan granules(清肺达原颗粒,QFDY),and to evaluate the acute and sub-chronic toxicity of QFDY.METHODS:Anti-inflammatory effects were evaluated by murine model of xylene induced ear edema in mice.Ear swelling degree was calculated and tumor necrosis factor-α,interleukin-1βand interleukin-6 were determined.Anti-tussive evaluations were carried out in the mouse cough model induced by ammonia liquor.Latent period cough and number of cough within 3 min were counted.In acute toxicity study,the rats were randomly divided into test group and solvent control group.Body weighs,food intakes and general clinical signs were monitored.In the sub-chronic toxicity study,QFDY was administered to rats at 0,4,8 and 16 g/kg per day for 28 and 30 d of post treatment was conducted.Mortalities,clinical signs,body weight changes,food intakes,ophthalmological examinations,hematological parameters,biochemical indicators,electrolyte indicators,urinalyses and histopathological examinations were monitored.RESULTS:QFDY significantly inhibited the development of ear edema in anti-inflammatory assay and decreased cough frequency caused by ammonia liquor.The results presented a dose-effect relationship.In acute toxicity study,no abnormality exhibited at dose of 24.0 g/kg per day during the 14-d observation period.In the sub-chronic toxicity study,higher reticulocyte count,lymphocyte and lower Cl-,blood urea nitrogen were analyzed compared with the solvent control group.But the differences were considered to be incidental and not clinically toxic.Obvious dose-effect relationship of urine color was observed,and the three test groups at the end of the experiments resulted in significant increase in urobilinogen,bilirubin,ketone body and urine leukocyte.However,all the positive indicators returned to normal in the recovery period.Therefore,no toxicological changes were found during the study period.CONCLUSION:QFDY showed significant anti-inflammatory and anti-tussive effects in mice.The lethal dose(LD50)of per oral QFDY in rats was estimated to be more than 24.0 g/kg per day and the no observed adverse effect level was over 16 g/kg per day,which suggested that QFDY is relatively safe for oral medication at the present dose on rats.Our experimental results provide a reference for the further development and research of QFDY.
基金Support by the Fundamental Scientific Research Funds for China Academy of Chinese Medical Sciences(Independent Topic Selection Project)
文摘OBJECTIVE:To observe the effects of New Dayuan powder(NDYP)on Methicillin-resistant Staphylococcus aureus(MRSA)biofilms and the embedded bacteria in vitro.METHODS:2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide(XTT)assays were used to study the effects of NDYP on developing MRSA biofilms:100μL of bacterial culture and100μL drug solution were added to wells of96-well plates.After 24 h of incubation,the plates were washed and XTT-phenazine methyl sulfate(PMS)was added to enable counting of the number of live bacteria in biofilms using a microplate reader.XTT assays were also used to explore the effects of NDYP on mature MRSA biofilms:100μL of bacterial culture were added to wells of 96-well plates.Bacteria were cultured in the plates for 24 h,and then drug solution was added.The plates were cultured for another 24 h,and then XTT-PMS was added to detect the number of live bacteria in the biofilms.Scanning electron microscopy(SEM)was used to observe the effects of NDYP on mature MRSA biofilms:washed and sterilized glass coverslips were added to 24-well plates.Bacterial culture was added.After 24 h of incubation,drug solution was added.After another 24 h of incubation,the samples were observed by SEM.RESULTS:XTT assays showed that the number of live bacteria in both developing and mature MRSA biofilms decreased significantly(P<0.01)after the administration of NDYP.SEM images showed that NDYP could destroy the structure of the bacteria and resulted in uneven thickness of MRSA biofilms.CONCLUSION:In vitro,NDYP has obvious inhibitory effects on the formation of MRSA biofilms and on mature biofilms.
文摘目的运用网络药理学方法对达原饮进行分析,探索达原饮治疗发热的相关靶点与通路,明确其作用机制与潜在治疗疾病,为后续研究提供指导。方法通过中药系统药理学数据库与分析平台(TCMSP)检索达原饮各组成药物的主要化学成分及作用靶点,参考类药五原则并结合口服生物利用度对成分靶点数据进行筛选,通过String数据库检索筛选后的靶点,得到达原饮作用靶点基因,在CTD数据库中检索发热(fever)相关作用靶点基因,对二者进行比较分析,取重复值,得到达原饮治疗发热相关靶点;在String数据库中检索达原饮治疗发热相关靶点,得到达原饮治疗发热相关通路;利用Cytoscape3.7.0软件对通路进行模块化分析,选择分值最高者,在WEB-based GEne SeT AnaLysis Toolkit中进行可视化分析。结果得到达原饮治疗发热相关靶点432个,相关通路75条,其中特异性疾病相关通路21条,非特异性疾病相关通路54条。结论达原饮治疗发热的作用机制涉及以代谢为中心的多种途径,可能通过多靶点复杂通路的共同干预起效。本研究就达原饮对于癌性发热的治疗补充了数据支持,并推测达原饮治疗神经退行性疾病具有可能性。
