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A novel molybdenite depressant for efficient selective flotation separation of chalcopyrite and molybdenite
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作者 Mengyao Qi Weijun Peng +3 位作者 Wei Wang Yijun Cao Longyu Zhang Yukun Huang 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第8期1179-1196,共18页
A novel small molecule depressant(M-DEP)was used to separate chalcopyrite and molybdenite via flotation.The results showed that M-DEP had an excellent selective depression on molybdenite,while had little effect on the... A novel small molecule depressant(M-DEP)was used to separate chalcopyrite and molybdenite via flotation.The results showed that M-DEP had an excellent selective depression on molybdenite,while had little effect on the flotation of chalcopyrite.The adsorption capacity of M-DEP on the surface of molybdenite was greater than that on chalcopyrite surface.The adsorption of M-DEP reduced the floatability of molybdenite and had less effect on the floatability of chalcopyrite,which was due to its different adsorption modes on the surface of the two minerals.Furthermore,the interaction between chalcopyrite and M-DEP was mainly chemical interaction,and almost all of the adsorbed M-DEP molecules were removed and replaced by sodium butyl xanthate(SBX).By contrast,hydrophobic interaction was the main way in which M-DEP was adsorbed on the molybdenite surface with little chemical interaction,which was less interfered by SBX addition.Therefore,M-DEP had a super selective depression on molybdenite.The study provided a novel depressant and approach for the deep separation of chalcopyrite and molybdenite via flotation. 展开更多
关键词 novel molecule depressant MOLYBDENITE CHaLCOPYRITE Flotation separation Selective depression
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Functional analysis of the novel mitochondrial tRNA^(Trp)and tRNA^(Ser(AGY))variants associated with type 2 diabetes mellitus
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作者 Yu Ding Xue-Jiao Yu +1 位作者 Qin-Xian Guo Jian-Hang Leng 《World Journal of Diabetes》 SCIE 2024年第8期1753-1763,共11页
BACKGROUND Mutations in mitochondrial tRNA(mt-tRNA)genes that result in mitochondrial dysfunction play important roles in type 2 diabetes mellitus(T2DM).We previously reported a large Chinese pedigree with maternally ... BACKGROUND Mutations in mitochondrial tRNA(mt-tRNA)genes that result in mitochondrial dysfunction play important roles in type 2 diabetes mellitus(T2DM).We previously reported a large Chinese pedigree with maternally inherited T2DM that harbors novel mt-tRNA^(Trp)A5514G and tRNA^(Ser(AGY))C12237T variants,however,the effects of these mt-tRNA variants on T2DM progression are largely unknown.AIM To assess the potential pathogenicity of T2DM-associated m.A5514G and m.C12237T variants at genetic,molecular,and biochemical levels.METHODS Cytoplasmic hybrid(cybrid)cells carrying both m.A5514G and m.C12237T variants,and healthy control cells without these mitochondrial DNA(mtDNA)variants were generated using trans-mitochondrial technology.Mitochondrial features,including mt-tRNA steady-state level,levels of adenosine triphosphate(ATP),mitochondrial membrane potential(MMP),reactive oxygen species(ROS),mtDNA copy number,nicotinamide adenine dinucleotide(NAD+)/NADH ratio,enzymatic activities of respiratory chain complexes(RCCs),8-hydroxy-deoxyguanine(8-OhdG),malondialdehyde(MDA),and superoxide dismutase(SOD)were examined in cell lines with and without these mt-tRNA variants.RESULTS Compared with control cells,the m.A5514G variant caused an approximately 35%reduction in the steady-state level of mt-tRNA^(Trp)(P<0.0001);however,the m.C12237T variant did not affect the mt-tRNA^(Ser(AGY))steady-state level(P=0.5849).Biochemical analysis revealed that cells with both m.A5514G and m.C12237T variants exhibited more severe mitochondrial dysfunctions and elevated oxidative stress than control cells:ATP,MMP,NAD+/NADH ratio,enzyme activities of RCCs and SOD levels were markedly decreased in mutant cells(P<0.05 for all measures).By contrast,the levels of ROS,8-OhdG and MDA were significantly increased(P<0.05 for all measures),but mtDNA copy number was not affected by m.A5514G and m.C12237T variants(P=0.5942).CONCLUSION The m.A5514G variant impaired mt-tRNA^(Trp)metabolism,which subsequently caused mitochondrial dysfunction.The m.C12237T variant did not alter the steady-state level of mt-tRNA^(Ser(AGY)),indicating that it may be a modifier of the m.A5514G variant.The m.A5514G variant may exacerbate the pathogenesis and progression of T2DM in this Chinese pedigree. 展开更多
关键词 Type 2 diabetes mellitus Mitochondrial tRNa genes novel variants Oxidative stress Mitochondrial dysfunctions
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Fatty acid binding protein 5 is a novel therapeutic target for hepatocellular carcinoma
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作者 Yan Li William Lee +3 位作者 Zhen-Gang Zhao Yi Liu Hao Cui Hao-Yu Wang 《World Journal of Clinical Oncology》 2024年第1期130-144,共15页
BACKGROUND Hepatocellular carcinoma(HCC)is an aggressive subtype of liver cancer and is one of the most common cancers with high mortality worldwide.Reprogrammed lipid metabolism plays crucial roles in HCC cancer cell... BACKGROUND Hepatocellular carcinoma(HCC)is an aggressive subtype of liver cancer and is one of the most common cancers with high mortality worldwide.Reprogrammed lipid metabolism plays crucial roles in HCC cancer cell survival,growth,and evolution.Emerging evidence suggests the importance of fatty acid binding proteins(FABPs)in contribution to cancer progression and metastasis;however,how these FABPs are dysregulated in cancer cells,especially in HCC,and the roles of FABPs in cancer progression have not been well defined.AIM To understand the genetic alterations and expression of FABPs and their associated cancer hallmarks and oncogenes in contributing to cancer malignancies.METHODS We used The Cancer Genome Atlas datasets of pan cancer and liver hepatocellular carcinoma(LIHC)as well as patient cohorts with other cancer types in this study.We investigated genetic alterations of FABPs in various cancer types.mRNA expression was used to determine if FABPs are abnormally expressed in tumor tissues compared to non-tumor controls and to investigate whether their expression correlates with patient clinical outcome,enriched cancer hallmarks and oncogenes previously reported for patients with HCC.We determined the protein levels of FABP5 and its correlated genes in two HCC cell lines and assessed the potential of FABP5 inhibition in treating HCC cells.RESULTS We discovered that a gene cluster including five FABP family members(FABP4,FABP5,FABP8,FABP9 and FABP12)is frequently co-amplified in cancer.Amplification,in fact,is the most common genetic alteration for FABPs,leading to overexpression of FABPs.FABP5 showed the greatest differential mRNA expression comparing tumor with non-tumor tissues.High FABP5 expression correlates well with worse patient outcomes(P<0.05).FABP5 expression highly correlates with enrichment of G2M checkpoint(r=0.33,P=1.1e-10),TP53 signaling pathway(r=0.22,P=1.7e-5)and many genes in the gene sets such as CDK1(r=0.56,P=0),CDK4(r=0.49,P=0),and TP53(r=0.22,P=1.6e-5).Furthermore,FABP5 also correlates well with two co-expressed oncogenes PLK1 and BIRC5 in pan cancer especially in LIHC patients(r=0.58,P=0;r=0.58,P=0;respectively).FABP5high Huh7 cells also expressed higher protein levels of p53,BIRC5,CDK1,CDK2,and CDK4 than FABP5low HepG2 cells.FABP5 inhibition more potently inhibited the tumor cell growth in Huh7 cells than in HepG2 cells.CONCLUSION We discovered that FABP5 gene is frequently amplified in cancer,especially in HCC,leading to its significant elevated expression in HCC.Its high expression correlates well with worse patient outcome,enriched cancer hallmarks and oncogenes in HCC.FABP5 inhibition impaired the cell viability of FABP5high Huh7 cells.All these support that FABP5 is a novel therapeutic target for treating FABP5high HCC. 展开更多
关键词 Hepatocellular carcinoma Fatty acid binding protein novel target aMPLIFICaTION Correlated expression
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Quantitative hepatitis B core antibody and quantitative hepatitis B surface antigen:Novel viral biomarkers for chronic hepatitis B management
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作者 Wattana Leowattana Pathomthep Leowattana Tawithep Leowattana 《World Journal of Hepatology》 2024年第4期550-565,共16页
The management of hepatitis B virus(HBV)infection now involves regular and appropriate monitoring of viral activity,disease progression,and treatment response.Traditional HBV infection biomarkers are limited in their ... The management of hepatitis B virus(HBV)infection now involves regular and appropriate monitoring of viral activity,disease progression,and treatment response.Traditional HBV infection biomarkers are limited in their ability to predict clinical outcomes or therapeutic effectiveness.Quantitation of HBV core antibodies(qAnti-HBc)is a novel non-invasive biomarker that may help with a variety of diagnostic issues.It was shown to correlate strongly with infection stages,hepatic inflammation and fibrosis,chronic infection exacerbations,and the presence of occult infection.Furthermore,qAnti-HBc levels were shown to be predictive of spontaneous or treatment-induced HBeAg and HBsAg seroclearance,relapse after medication termination,re-infection following liver transplantation,and viral reactivation in the presence of immunosuppression.qAnti-HBc,on the other hand,cannot be relied on as a single diagnostic test to address all problems,and its diagnostic and prognostic potential may be greatly increased when paired with qHBsAg.Commercial qAnti-HBc diagnostic kits are currently not widely available.Because many methodologies are only semi-quantitative,comparing data from various studies and defining universal cut-off values remains difficult.This review focuses on the clinical utility of qAnti-HBc and qHBsAg in chronic hepatitis B management. 展开更多
关键词 Quantitative hepatitis B core antibody Quantitative hepatitis B surface antigen Chronic hepatitis B management novels viral biomarkers
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Analyzing the Adaptation of Rebecca from the Novel to the Film in Light of the Black-and-White
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作者 Jing Wang 《Review of Educational Theory》 2024年第1期20-26,共7页
Classical Chinese aesthetics is based on Chinese philosophy.The characteristic of classical Chinese aesthetics regards“artistic conception”as one of the basic aesthetic categories.Chinese art speaks highly of the ch... Classical Chinese aesthetics is based on Chinese philosophy.The characteristic of classical Chinese aesthetics regards“artistic conception”as one of the basic aesthetic categories.Chinese art speaks highly of the charm of the black-and-white.During the adaptation,there is the same beauty in the black-and-white photography and the“color without color”of Chinese paintings.The“artistic conception”is similar between them from the aesthetic perspective.Therefore,the unique“artistic conception”of the novel should be mastered,if the adapter aims to retain the“soul”of it.In other words,film adaptation not only considers“similarity in form”,but also draws much attention to“similarity in spirit”.The latter is the essence of film adaptation. 展开更多
关键词 REBECCa the black-and white novel FILM artistic conception
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Prevention and Nursing of Adverse Reactions of Novel Coronavirus Inactivated Vaccine (Vero Cells)
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作者 Jingyu He Muyang Bian +1 位作者 Jianan Du Liping Wang 《Journal of Clinical and Nursing Research》 2024年第5期100-107,共8页
Objective:To discuss and analyze the causes of adverse reactions caused by the inactivated novel coronavirus vaccine(Vero cells),and to propose methods of prevention and care.Methods:A questionnaire was used to random... Objective:To discuss and analyze the causes of adverse reactions caused by the inactivated novel coronavirus vaccine(Vero cells),and to propose methods of prevention and care.Methods:A questionnaire was used to randomly select 229 adults who were vaccinated with the inactivated novel coronavirus vaccine(Vero cells)at Xi’an People’s Hospital(Xi’an Fourth Hospital).The adverse reactions were statistically analyzed.Results:Among the 229 adults vaccinated with the inactivated novel coronavirus vaccine(Vero cells),30 experienced vaccination reactions.The main reaction was local induration at the inoculation site,and dizziness was the primary systemic symptom.Conclusion:To reduce the incidence of adverse reactions to the inactivated novel coronavirus vaccine(Vero cells),it is necessary to effectively evaluate the health status of adults before vaccination,select the correct vaccination site,and strictly implement the rules of 3-inspections,7-checks,and 1-verification.Standardizing the operation process and providing thorough health education after vaccination can effectively reduce the occurrence of adverse reactions. 展开更多
关键词 novel coronavirus inactivated vaccine(Vero cells) VaCCINaTION adverse reactions nursing
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The Application of Newmark’s Metaphor Theory in Novel Translation--A Case Study of“the Jade King:History of a Chinese Muslim Family”
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作者 Yuchen Xiao 《教育研究前沿(中英文版)》 2024年第3期100-112,共13页
This paper examines the metaphorical part of“The Jade King:History of a Chinese Muslim Family”,Hoda’s novel,under the guidance of Newmark’s theory of metaphor.Newmark proposes six categories of metaphors,namely De... This paper examines the metaphorical part of“The Jade King:History of a Chinese Muslim Family”,Hoda’s novel,under the guidance of Newmark’s theory of metaphor.Newmark proposes six categories of metaphors,namely Dead Metaphor,ClichéMetaphor,Stock or Standard Metaphor,Adapted Metaphor,Recent Metaphor,Original Metaphor;and seven strategies of metaphor translation,including reproducing the same image in the target language,replacing the image in the source language with a standard target language image,translation of metaphor by simile,translation of metaphor(or simile)by simile plus sense,conversion of metaphor to sense,deletion and same metaphor combined with sense.They can provide a strong theoretical support for analyzing the expressions of metaphors in novels and their translation methods.By deeply analyzing the expressions of metaphor and its translation methods in novels,it not only helps to understand the metaphorical meaning and cultural connotation of the original works,but also helps to explore the cultural differences and challenges faced by English and Chinese bilingualism in the process of metaphor translation,thereby significantly improving the translation level and promoting the development of translation research. 展开更多
关键词 METaPHOR novel Translation “the Jade King:History of a Chinese Muslim Family”
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基于Transformer的多尺度遥感语义分割网络 被引量:1
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作者 邵凯 王明政 王光宇 《智能系统学报》 CSCD 北大核心 2024年第4期920-929,共10页
为了提升遥感图像语义分割效果,本文针对分割目标类间方差小、类内方差大的特点,从全局上下文信息和多尺度语义特征2个关键点提出一种基于Transformer的多尺度遥感语义分割网络(muliti-scale Transformer network,MSTNet)。其由编码器... 为了提升遥感图像语义分割效果,本文针对分割目标类间方差小、类内方差大的特点,从全局上下文信息和多尺度语义特征2个关键点提出一种基于Transformer的多尺度遥感语义分割网络(muliti-scale Transformer network,MSTNet)。其由编码器和解码器2个部分组成,编码器包含基于Transformer改进的视觉注意网络(visual attention network,VAN)主干和基于空洞空间金字塔池化(atrous spatial pyramid pooling, ASPP)结构改进的多尺度语义特征提取模块(multi-scale semantic feature extraction module, MSFEM)。解码器采用轻量级多层感知器(multi-layer perception,MLP)配合编码器设计,充分分析所提取的包含全局上下文信息和多尺度表示的语义特征。MSTNet在2个高分辨率遥感语义分割数据集ISPRS Potsdam和LoveDA上进行验证,平均交并比(mIoU)分别达到79.50%和54.12%,平均F1-score(m F1)分别达到87.46%和69.34%,实验结果验证了本文所提方法有效提升了遥感图像语义分割的效果。 展开更多
关键词 遥感图像 语义分割 卷积神经网络 TRaNSFORMER 全局上下文信息 多尺度感受野 编码器 解码器
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线性分组码的GRAND算法研究
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作者 史治平 杨娟 +1 位作者 李坤阳 王玥 《移动通信》 2024年第5期49-54,共6页
随着5G通信技术的发展与应用,高可靠低时延通信场景下的短包信道编译码技术受到了广泛关注。GRAND是一种通用的最大似然译码算法,与传统译码算法不同,GRAND算法通过噪声猜测和码本查询来确定发送码字,具有高效的硬件实现结构。介绍了GR... 随着5G通信技术的发展与应用,高可靠低时延通信场景下的短包信道编译码技术受到了广泛关注。GRAND是一种通用的最大似然译码算法,与传统译码算法不同,GRAND算法通过噪声猜测和码本查询来确定发送码字,具有高效的硬件实现结构。介绍了GRAND及其改进算法的基本原理,通过仿真分析了不同GRAND算法的误码率性能;针对5G NR的LDPC短码以及PAC短码给出了GRAND算法的设计方案,为未来移动通信的短码设计提供参考。 展开更多
关键词 短码 GRaND算法 线性分组码
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血清MG7-Ag水平检测与新型胃癌评分系统在胃癌高危人群风险评估中的价值
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作者 张鹏飞 杨小飞 +5 位作者 瞿焱 张萌 张强林 李梦龙 金靓 许怀利 《现代肿瘤医学》 CAS 2024年第8期1458-1461,共4页
目的:研究血清MG7-Ag与新型胃癌评分系统在胃癌高危人群风险评估中的临床价值。方法:纳入2021年1月至2022年12月在本院接受胃镜检查及活检的163例胃癌癌前病变患者和42例胃癌患者作为研究对象。收集一般资料,包括年龄、性别、血清幽门... 目的:研究血清MG7-Ag与新型胃癌评分系统在胃癌高危人群风险评估中的临床价值。方法:纳入2021年1月至2022年12月在本院接受胃镜检查及活检的163例胃癌癌前病变患者和42例胃癌患者作为研究对象。收集一般资料,包括年龄、性别、血清幽门螺杆菌抗体(呼气试验C13/C14)、胃蛋白酶原I/胃蛋白酶原II比值(PGR)、胃泌素17(G17)、内镜检查及病理诊断结果:根据新型胃癌筛查评分系统将入组患者分为低危组、中危组、高危组,采用酶联免疫吸附法进行MG7-Ag检测,分析影响胃癌诊断的影响因素,评价MG7-Ag和新型胃癌评分系统在胃癌高危人群风险评估中的价值。结果:胃癌癌前病变和胃癌组患者一般资料差异无统计学意义,具有可比性。所有205例患者中,新型胃癌评分系统分为高危34例、中危63例和低危108例,其中胃癌检出率分别为24(73.53%)例、13(20.63%)例、5(4.63%)例。以MG7-Ag表达的中位数分为高表达组和低表达组,其中高表达组41例、低表达组164例,其中胃癌检出率分别为33(80.49%)例、9(5.49%)例。单因素和多因素分析显示,新型胃癌评分系统和MG7-Ag检测均为影响胃癌诊断的影响因素之一。通过ROC曲线分析新型胃癌评分系统和MG7-Ag检测在癌前病变和胃癌中的诊断价值,结果显示,新型胃癌评分系统的曲线下面积(AUC)为0.71(95%CI:0.65~0.74),MG7-Ag检测的AUC为0.78(95%CI:0.75~0.80),两者联合诊断的AUC为0.86(95%CI:0.81~0.90),两者联合诊断的价值明显优于单独诊断。结论:血清MG7-Ag与新型胃癌评分系统联合诊断在筛选胃癌高危人群中的价值明显优于单独检测,对未来胃癌癌前病变和胃癌的诊断具有很高的临床指导价值。 展开更多
关键词 新型胃癌筛查评分 MG7-aG 胃癌前病变
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AI绘画软件的创作特征研究——以绘画软件Novel AI生成的动漫人物形象为例 被引量:10
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作者 余青龙 《信阳师范学院学报(哲学社会科学版)》 2023年第3期127-132,共6页
近年来,随着扩散模型、Lora模型、Controlnet等技术应用于绘画领域,现今的AI绘画软件已经能够生成完整的动漫人物,具备了较高的可控性和精确性。文章通过分析AI绘画软件“Novel AI”生成的动漫人物形象,总结了这一AI软件表现出的绘画创... 近年来,随着扩散模型、Lora模型、Controlnet等技术应用于绘画领域,现今的AI绘画软件已经能够生成完整的动漫人物,具备了较高的可控性和精确性。文章通过分析AI绘画软件“Novel AI”生成的动漫人物形象,总结了这一AI软件表现出的绘画创作特征,并在此基础上评估了现今人工智能技术对于动漫创作产生的影响。 展开更多
关键词 aI绘画 novel aI 动漫人物
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新型查尔酮衍生物抗乳腺癌活性的3D-QSAR模型构建及分子设计
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作者 陈艳 冯惠 +1 位作者 冯长君 堵锡华 《南京理工大学学报》 CAS CSCD 北大核心 2024年第2期248-252,共5页
为了获得较高抗乳腺癌活性的新型化合物,对18个新型查尔酮衍生物抗乳腺癌活性(pIC_(50))进行了三维定量构效关系(3D-QSAR)研究。其中14个化合物作为训练集用于构建3D-QSAR模型,其余化合物(含模板分子)作为测试集对所建模型进行验证。所... 为了获得较高抗乳腺癌活性的新型化合物,对18个新型查尔酮衍生物抗乳腺癌活性(pIC_(50))进行了三维定量构效关系(3D-QSAR)研究。其中14个化合物作为训练集用于构建3D-QSAR模型,其余化合物(含模板分子)作为测试集对所建模型进行验证。所建3D-QSAR模型的交叉验证系数R^(2)_(CV)为0.569,非交叉验证系数R^(2)为0.974,说明所建模型具有良好的稳定性和预测能力。该模型中立体场、静电场对pIC_(50)的贡献分别为58.8%和41.2%,表明影响该类化合物抗肿瘤活性的主要因素是取代基的疏水性、空间位阻和电荷分布。通过对模型的分析,设计了5个具有较高pIC_(50)的新化合物,有待通过后续医学实验加以验证。 展开更多
关键词 新型查尔酮衍生物 抗乳腺癌活性 三维定量构效关系 分子设计
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基于视觉Transformer和双解码器的红外小目标检测方法
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作者 代少升 刘科生 +3 位作者 黄炼 贺自强 毛兴华 任汶皓 《红外技术》 CSCD 北大核心 2024年第9期1070-1080,共11页
当前基于卷积神经网络的红外小目标检测方法在编码器阶段受限于感受野,且解码器在多尺度特征融合中缺乏有效的特征交互。本文提出了一种基于编码器-解码器结构的新方法,针对现有红外小目标检测方法中的问题进行改进。该方法使用视觉Tran... 当前基于卷积神经网络的红外小目标检测方法在编码器阶段受限于感受野,且解码器在多尺度特征融合中缺乏有效的特征交互。本文提出了一种基于编码器-解码器结构的新方法,针对现有红外小目标检测方法中的问题进行改进。该方法使用视觉Transformer作为编码器,能够有效地提取红外小目标图像的多尺度特征。视觉Transformer是一种新兴的深度学习架构,其通过自注意力机制捕捉图像中像素之间的全局关系,以处理长程依赖性和上下文信息。此外,本文还设计了一个由交互式解码器和辅助解码器组成的双解码器模块,旨在提高解码器对红外小目标的重构能力。该双解码器模块能够充分利用不同特征之间的互补信息,促进深层特征和浅层特征之间的交互,并通过将两个解码器的结果进行叠加,以更好地重构红外小目标。在广泛使用的公共数据集上的实验结果表明,本文提出的方法在F1和mIoU两个评价指标上的性能优于其他对比方法。 展开更多
关键词 红外小目标检测 视觉Transformer 多尺度特征融合 编解码结构
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一种基于Polar译码度量选择的第三方高效PDCCH盲检方法
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作者 王霄峻 马晓静 黄钰华 《Journal of Southeast University(English Edition)》 EI CAS 2024年第1期97-104,共8页
为研究和设计面向第三方场景的高效盲检方法,提出了一种基于Polar译码度量选择的第三方高效PDCCH盲检方法,其技术路线包括Polar译码算法与物理的下行控制信道(PDCCH)盲检算法2个板块.基于Polar译码盲检算法,引入一种基于下行控制信息(D... 为研究和设计面向第三方场景的高效盲检方法,提出了一种基于Polar译码度量选择的第三方高效PDCCH盲检方法,其技术路线包括Polar译码算法与物理的下行控制信道(PDCCH)盲检算法2个板块.基于Polar译码盲检算法,引入一种基于下行控制信息(DCI)长度的Polar译码度量,提出了改进的基于Polar译码度量选择的第三方盲检方法.基于PDCCH盲检算法,引入一种重排序盲检算法.将改进的Polar译码算法与重排序盲检算法有机结合,提出面向第三方场景的高效PDCCH盲检方法.基于MATLAB平台搭建5G PDCCH盲检仿真链路,对所提方法进行验证与分析.结果表明,该方法能够同时有效减小PDCCH盲检次数与DCI候选长度数量,在保证目标捕获准确率的前提下提高盲检效率. 展开更多
关键词 Polar译码 物理的下行控制信道 盲检 下行控制信息
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Clinical and genetic characteristics of retinoblastoma patients in a single center with four novel RB1 variants 被引量:1
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作者 Özge Vural Hatice Tuba Atalay +5 位作者 Gulsum Kayhan Bercin Tarlan Merve Oral Arzu Okur Faruk GüçlüPınarlı Ceyda Karadeniz 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第8期1274-1279,共6页
AIM:To assess the clinical and genetic characteristics of children diagnosed with retinoblastoma(RB)at Gazi University Faculty of Medicine’s Department of Pediatric Oncology.METHODS:All cases diagnosed with RB and re... AIM:To assess the clinical and genetic characteristics of children diagnosed with retinoblastoma(RB)at Gazi University Faculty of Medicine’s Department of Pediatric Oncology.METHODS:All cases diagnosed with RB and received treatment and follow-up in the Ophthalmology and Pediatric Oncology Department,October 2016 to May 2021 were evaluated retrospectively.The RB1 gene was analyzed by next-generation sequencing(NGS)technique in DNAs obtained from peripheral blood samples of the patients.RESULTS:This study included 53 cases with 67 RBaffected eyes during the study period.The mean age was 24.6(median:18.5,range:3–151)mo.There were 15(22.3%)Group D eyes and 39(58.2%)Group E eyes.The RB1 gene was sequenced by the NGS method in 19 patients.Heterozygous RB1:NM_000321.3:c.54_76del(p.Glu19AlafsTer4)variant was detected in a 15-month-old female with bilateral RB.Heterozygous RB1:NM_000321.3:c.1814+3A>T variant was detected in a 5.5-month-old male with bilateral RB.The intronic RB1:NM_000321.3:c.1332+4A>G variant was detected in patient 14,a 13-month-old male with unilateral RB.The RB1:NM_000321.3:c.575_576del(p.Lys192SerfsTer10)variant was found in an 18-month-old female with an allele frequency of 37%.These variants have not been reported in the literature and mutation databases.CONCLUSION:Four novel variants are described and one of them is found in two different patients.This data is crucial for assessing prognosis.It serves as a guide for estimating the long-term risk of secondary malignancy as well as the short-term risk of developing additional malignancies in the same eye and the other eye. 展开更多
关键词 RETINOBLaSTOMa RB1 novel mutations next generation sequencing
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Identification and functional analyses of a novel FOXL2 pathogenic variant causing blepharophimosis, ptosis, and epicanthus inversus syndrome 被引量:1
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作者 Yu-Cheng Yan Lu Zhou Jin-Cai Fan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第5期680-686,共7页
AIM: To discover the molecular pathogenic basis of the blepharophimosis, ptosis, and epicanthus inversus syndrome(BPES), and to predict the clinical subtype according to in vitro experiments, which is significant to t... AIM: To discover the molecular pathogenic basis of the blepharophimosis, ptosis, and epicanthus inversus syndrome(BPES), and to predict the clinical subtype according to in vitro experiments, which is significant to the prognosis.METHODS: A 3-year-old sporadic female patient with typical clinical manifestations of BPES was enrolled. The coding region of forkhead box L2(FOXL2) gene was sequenced, and the functional assays were performed in vitro by Western blotting, subcellular localization experiment, luciferase reporter assay, and quantitative realtime polymerase chain reaction.RESULTS: A novel FOXL2 point pathogenic variant(c.274G>T) was detected, resulting in a truncated protein(p.E92*). Functional studies demonstrated that the FOXL2 pathogenic variant induced the subcellular mislocalization and the abnormal transcriptional activity on promoters of the steroidogenic acute regulatory protein(StAR or STARD1) gene and the odd-skipped related 2 transcription factor(OSR2) gene.CONCLUSION: A novel pathogenic variant is identified to expand the spectrum of the known FOXL2 mutations. The in vitro experiments provide reference data and more insights to the molecular pathogenesis of BPES. The predicted high risk of ovarian insufficiency makes it significant for the patient enrolled to have further follow-up and therapy concerning female endocrinology. 展开更多
关键词 novel mutation pathogenic variant blepharophimosis-ptosis-epicanthus inversus syndrome FOXL2
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A novel Ti-5.55Al-6.70Zr-1.50V-0.70Mo-3.41Nb-0.21Si alloy designed using cluster-plus-glue-atom model for laser additive manufacturing 被引量:1
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作者 Tian-yu Liu Yan-chun Lou +5 位作者 Shuang Zhang Zhi-hao Zhu Jun Zhao Shi-bing Liu Kun Shi Ning Zhao 《China Foundry》 SCIE CAS CSCD 2023年第5期414-422,共9页
A novel Ti-5.55Al-6.70Zr-1.50V-0.70Mo-3.41Nb-0.21Si alloy was designed using the cluster formula approach(cluster-plus-glue-atom model)and prepared by laser melting deposition(LMD).Its composition formula 12[Al-Ti_(12... A novel Ti-5.55Al-6.70Zr-1.50V-0.70Mo-3.41Nb-0.21Si alloy was designed using the cluster formula approach(cluster-plus-glue-atom model)and prepared by laser melting deposition(LMD).Its composition formula 12[Al-Ti_(12)](AlTi_(2))+5[Al_(0.8)Si_(0.2)-Ti_(12)Zr_(2)](V_(0.8)Mo_(0.2)Nb_(1)Ti)features an enhancedβ-Ti via co-alloying of Zr,V,Mo,Nb and Si.The experimental results show that the cluster formula ofαandβphases in the novel alloy are respectivelyα-[Al-Ti_(11.5)Zr_(0.5)](Al_(1)Ti_(2))andβ-[Al_(0.8)Si_(0.2)-Ti_(13.2)Zr_(0.8)](V_(1)Mo_(0.4)Nb_(1.6)),both containing Zr elements.The fitted composition via the α andβphase cluster formulas has little difference with the actual alloy composition,suggesting that the validity of cluster-plus-glue-atom model in the alloy composition design.After hot isostatic pressing(HIP),both the Ti-6Al-4V and the novel alloy by LMD are characterized by prior-βcolumnar grains,while the typical<100>texture disappears.Compared with Ti-6Al-4V,Ti-5.55Al-6.70Zr-1.50V-0.70Mo-3.41Nb-0.21Si alloy exhibits a combination of higher strength(1,056 MPa)and higher ductility(14%)at room temperature and higher strength(580 MPa)at 550℃ after HIP,and can potentially serves as LMD materials. 展开更多
关键词 additive manufacturing laser melting deposition novel titanium alloy composition design cluster-plus-glue-atom model
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Novel combined endoscopic and laparoscopic surgery for advanced T2 gastric cancer:Two case reports 被引量:1
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作者 Jian-Hua Dai Feng Qian +7 位作者 Lei Chen Sen-Lin Xu Xiao-Feng Feng Hong-Bo Wu Yao Chen Zhi-Hong Peng Pei-Wu Yu Gui-Yong Peng 《World Journal of Clinical Cases》 SCIE 2023年第9期2029-2035,共7页
BACKGROUND The standard treatment for advanced T2 gastric cancer(GC)is laparoscopic or surgical gastrectomy(either partial or total)and D2 lymphadenectomy.A novel combined endoscopic and laparoscopic surgery(NCELS)has... BACKGROUND The standard treatment for advanced T2 gastric cancer(GC)is laparoscopic or surgical gastrectomy(either partial or total)and D2 lymphadenectomy.A novel combined endoscopic and laparoscopic surgery(NCELS)has recently been proposed as a better option for T2 GC.Here we describe two case studies demonstrating the efficacy and safety of NCELS.CASE SUMMARY Two T2 GC cases were both resected by endoscopic submucosal dissection and full-thickness resection and laparoscopic lymph nodes dissection.This method has the advantage of being more precise and minimally invasive compared to current methods.The treatment of these 2 patients was safe and effective with no complications.These cases were followed up for nearly 4 years without recurrence or metastasis.CONCLUSION This novel method provides a minimally invasive treatment option for T2 GC,and its potential indications,effectiveness and safety needs to be further evaluated in controlled studies. 展开更多
关键词 novel combined endoscopic and laparoscopic surgery T2 gastric cancer Endoscopic submucosal dissection and full-thickness resection Laparoscopic lymph nodes
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犬新孢子虫miRNAs的鉴定与分析
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作者 李京宇 陈金铭 +6 位作者 张明一 赵姗姗 陶德良 宋军科 杨新 樊莹莹 赵光辉 《畜牧兽医学报》 CAS CSCD 北大核心 2024年第7期3085-3093,共9页
为鉴定犬新孢子虫(Neospora caninum)特异表达的miRNAs,并研究其在宿主细胞中的表达情况,本研究收集N.caninum速殖子感染的山羊子宫内膜上皮细胞(caprine endometrial epithelial cells,EECs)24和48 h的样本,用Illumina高通量测序平台... 为鉴定犬新孢子虫(Neospora caninum)特异表达的miRNAs,并研究其在宿主细胞中的表达情况,本研究收集N.caninum速殖子感染的山羊子宫内膜上皮细胞(caprine endometrial epithelial cells,EECs)24和48 h的样本,用Illumina高通量测序平台和生物信息学分析犬新孢子虫特异表达的miRNAs及其体外表达情况,并研究了一个上调的miRNA novel3_mature对犬新孢子虫在山羊EECs增殖的影响。结果发现,共鉴定出3404个犬新孢子虫特异表达的miRNAs(155个新预测miRNAs),其中77个(47个上调和30个下调)miRNAs显著差异表达;随机选取7个显著差异表达的miRNAs进行实时荧光定量PCR分析,结果与高通量测序结果一致;预测分析发现61个miRNAs的3978个靶基因,功能富集分析发现这些靶基因可能主要参与MAPK信号通路、突触导向、黏着斑、cMAP信号通路、钙信号通路等;转染novel3_mature的模拟物显著促进犬新孢子虫在山羊EECs中的增殖。犬新孢子虫存在多个特异表达的miRNAs,其在虫体增殖中发挥重要作用,研究成果为深入理解犬新孢子虫胞内生存提供了新的思路。 展开更多
关键词 犬新孢子虫 山羊子宫内膜上皮细胞 MIRNaS novel3_mature 增殖
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动态扰动辅助的串行抵消双比特翻转Polar译码算法
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作者 曾俏丽 陈海强 +3 位作者 周泉 刘远博 孙友明 黎相成 《电讯技术》 北大核心 2024年第1期126-131,共6页
针对串行抵消翻转译码算法(Successive Cancellation Flip,SCF)受限于单比特翻转而性能提升有限问题,提出了一种双比特翻转译码算法(Successive Cancellation Flip with 2 Bits,SCF2)。针对SCP算法扰动方差初始值固定的问题,设计了一种... 针对串行抵消翻转译码算法(Successive Cancellation Flip,SCF)受限于单比特翻转而性能提升有限问题,提出了一种双比特翻转译码算法(Successive Cancellation Flip with 2 Bits,SCF2)。针对SCP算法扰动方差初始值固定的问题,设计了一种扰动方差可随码长和码率变化的改进SCP算法。在此基础上,结合翻转和扰动机制,提出了一种动态扰动辅助的串行抵消双比特翻转(Dynamic Perturbation-Aided SCF2,DPA-SCF2)译码算法,并对其译码复杂度和性能进行了分析。仿真结果显示,相比于列表长度为4的循环冗余校验辅助串行抵消列表(Cyclic Redundancy Check Aided Successive Cancellation List,CA-SCL)译码算法,所提算法最大可获得约0.5 dB的性能增益。 展开更多
关键词 Polar码 串行抵消翻转(SCF)译码 串行抵消扰动(SCP)译码 动态扰动
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