A potential impact of A Disintegrin and Metalloproteinase Domain-Like Protein Decysin-1(ADAMDEC1)on clear cell renal cell carcinoma propagation

Clear cell renal cell carcinoma(KIRC)is the most common and aggressivemalignancy subtype of renal neoplasm that arises from proximal convoluted tubules.It is characterized by poor clinical outcomes and high mortality of patients due to the lack of specific biomarkers for varying stages of the disease and no effective treatment.Proteases are associated with the development of several malignant tumors in humans by their ability to degrade extracellular matrices,facilitating metastasis.Herein,differentially expressed genes in KIRC cases compared to healthy kidneys were screened out from the Gene Expression Profiling Interactive Analysis(GEPIA)database.This data was applied to determine the most elevated protease in KIRC and as a result,A Disintegrin and Metalloproteinase Domain-Like Protein Decysin-1(ADAMDEC1)was selected.This expression pattern was exclusive for KIRC and not observed for papillary and chromophobe renal cell carcinomas,in which ADAMDEC1 was at the same level in tumors and non-cancer specimens.Furthermore,the ADAMDEC1 significant increase was detected in the fourteen other human malignancies compared to healthy samples,which suggested its strong involvement in cancer development.Next,GEPIA and Pathology Atlas correlated ADAMDEC1 high expression with more advanced tumor grade and shorter survival of KIRC patients.Xena Functional Genomics Explorer presented that ADAMDEC1 could be hypermethylated in some tumor cases and one somatic mutation in the gene sequence was detected.Finally,a Search Tool for the Retrieval of Interacting Genes/Proteins;STRING base was utilized to predict the interactions of ADAMDEC1 with other molecules and construct the signaling network.In summary,ADAMDEC1 showed the tremendous potential to be the predictive marker for the KIRC and its development.Therefore,this review with data analysis can be a good base for further in vitro and in vivo research that experimentally can confirm the ADAMDEC1 as prognostic biomarkers and therapeutic target of KIRC.

脑膜瘤组织中Ki-67蛋白、ADAMDEC1蛋白表达及其与临床病理特征相关性分析

目的:探讨脑膜瘤组织中增殖细胞核抗原(Ki-67)蛋白、解聚素金属蛋白酶癸蛋白1(ADAMDEC1)蛋白表达及与临床病理特征间的关系。方法:选取2018年1月至2021年1月本院的82例脑膜瘤患者作为观察组,另选同期因外伤行颅脑手术的患者60例作为对照组。所有患者均行手术治疗,术中采集脑膜瘤标本及正常脑组织标本,均行免疫组化染色法检测Ki-67、ADAMDEC1表达,比较两组Ki-67、ADAMDEC1表达差异;并比较不同Ki-67蛋白、ADAMDEC1蛋白表达患者年龄、性别、肿瘤大小、WHO分级、瘤周水肿、脑膜尾征、骨侵犯等病理特征方面差异;所有脑膜瘤患者均随访2年,记录疾病复发情况,并分析疾病复发与Ki-67蛋白、ADAMDEC1蛋白表达间的关系。结果:观察组Ki-67蛋白、ADAMDEC1蛋白阳性表达率高于对照组,差异有统计学意义(P<0.05);Ki-67蛋白阳性组、ADAMDEC1蛋白阳性组WHO分级Ⅱ~Ⅲ级、瘤周水肿、骨侵犯占比高于Ki-67蛋白阴性组、ADAMDEC1蛋白阴性组,差异有统计学意义(P<0.05);82例患者随访2年,共出现11例复发,复发率为13.41%(11/82);Ki-67蛋白阳性组、ADAMDEC1蛋白阳性组复发率略高于Ki-67蛋白阴性组、ADAMDEC1蛋白阴性组,差异无统计学意义(P>0.05)。结论:脑膜瘤组织内Ki-67蛋白、ADAMDEC1蛋白表达水平高,且与WHO分级、瘤周水肿、骨侵犯存在密切关系,可辅助临床完善早期治疗方案,改善患者预后。

ADAMDEC1蛋白在脑膜瘤中的表达及意义

目的检测脑膜瘤组织中崩解素和金属蛋白酶结构域样蛋白decysin-1(a disintegrin and metalloproteases-like decysin-1,ADAMDEC1)的表达,探讨其与脑膜瘤病理特征的相关性。方法收集2015年7月至2017年12月在西南医科大学附属中医医院神经外科住院并行手术切除治疗、病理诊断结果为脑膜瘤的脑膜瘤组织标本32例,设为脑膜瘤组;同期取自重型颅脑外伤行内外减压术的正常脑组织标本12例,设为对照组。应用免疫组织化学法检测ADAMDEC1的表达。结果ADAMDEC1蛋白在脑膜瘤组织中的表达显著高于正常脑膜组织,差异均有统计学意义(P<0.05)。脑膜瘤WHO分级与ADAMDEC1的表达呈正相关,差异均有统计学意义(P<0.05)。脑膜瘤的侵袭性与ADAMDEC1蛋白表达呈正相关,差异均有统计学意义(P<0.05)。结论ADAMDEC1在脑膜瘤组织中高表达,其阳性表达高低与脑膜瘤WHO分级、脑膜瘤的侵袭性存在关联,ADAMDEC1可作为脑膜瘤恶性程度分级评判指标,ADAMDEC1蛋白检测在脑膜瘤患者的诊断和预后中具有重要意义。