Transcriptomic and metabolomic analysis of the effects of Zhenwu decoction on kidney yang deficiency pattern in chronic kidney disease

Objective:To explore the kidney yang deficiency pattern(KYDP)in a chronic kidney disease(CKD)rat model and the mechanisms underlying the effects of Zhenwu decoction(ZWD)by conducting tran-scriptomic and metabolomic analyses.Methods:Adriamycin(ADR)combined with hydrocortisone(HC)was used to induce CKD with KYDP in rats.ADR was injected into the tail vein twice.HC was injected intramuscularly for 8 weeks.ZWD was administered by gavage for 8 weeks.The general condition was observed,24-h urine protein was detected,serum corticosterone,triiodothyronine,thyroxine,TSH,testosterone,cAMP,and cGMP levels were determined,and pathological analysis was conducted.Transcriptomic and metabolomic analyses were conducted to screen differentially expressed genes(DEGs),differentially expressed metabolites(DEMs),and differentially expressed pathways(DEPs).The core DEMs and DEGs were input to Metab-oanalyst 5.0 to identify the pathways affected by ZWD.Results:In the HC group,KYDP symptoms were observed.Compared with control group,the levels of 24-h urine protein,TSH,and cGMP significantly increased(all P<0.01),and corticosterone,triiodothyronine,thyroxine,and cAMP significantly decreased(all P<0.01)in the HC group.After ZWD intervention,the levels of above-mentioned indicators could be reversed to some extent.Pathological analysis in the HC group revealed kidney lesions.DEGs in the ZWD group were mainly associated with pathways such as nucleotide synthesis and endocrine pathways.In the ZWD group,differences in biosynthesis of unsat-urated fatty acids and butanoate metabolism were observed.The following pathways were significantly affected by ZWD:arachidonic acid metabolism,valine,leucine,and isoleucine biosynthesis,linoleic acid metabolism,and alpha-linolenic acid metabolism.Conclusion:ZWD can be used to treat KYDP in CKD through regulating arachidonic acid metabolism,valine,leucine,and isoleucine biosynthesis,linoleic acid metabolism,and alpha-linolenic acid metabolism.

Clinical Study of Acupotomy Trinity Lysis on Cervical Spondylotic Myelopathy with Liver and Kidney Deficiency Syndrome

Objective:To compare the therapeutic effects of acupotomy trinity lysis and traditional acupotomy on cervical spondylotic myelopathy.Methods:A total of 205 patients with cervical spondylotic myelopathy of liver and kidney deficiency syndrome were randomly divided into the experimental group(105 cases)and the control group(100 cases).The experimental group was relaxed with acupotomy in three positions:Heaven(tian),Human(ren)and Earth(di).Traditional acupotomy was used to relax Ashi acupoints of the affected vertebra in the control group.One treatment was conducted in one week,and the duration of one course of treatment was three weeks.The VAS,JOA score and NDI index were observed after treatment.Results:Before and after treatment,the total treatment efficiency of the treatment group was 95.23%,and that of the control group was 80.00%,there was significant difference between the two groups,P<0.05;Before operation,there was no significant difference in JOA score,NDI index score,and VAS score between the treatment group and the control group(P>0.05);there was no significant difference after 1 week(P>0.05),but there were significant differences between the two groups 2 weeks and 3 weeks after operation(P<0.05).Conclusion:Acupotomy trinity lysis is a safe,effective and economical treatment for cervical spondylotic myelopathy.

Effect of Huqian Wan on liver-Yin and kidney-Yin deficiency patterns in patients with knee osteoarthritis

OBJECTIVE: To observe the curative effect of Huqian Wan on liver and kidney-Yin deficiency knee osteoarthritis(KOA).METHODS: One hundred patients were randomly divided into a treatment(50 patients) and control group(50 patients). In the treatment group, patients orally took the Chinese medicine Huqian Wan. Control group patients orally took Votalin, 75 mg, once a day, for 8 weeks. The visual analog scale(VAS), Western Ontario and Mc Master University Osteoarthritis Index(WOMAC), and Medical Outcomes Study Short Form 36-Item Health Survey(SF36) were used to evaluate the curative effect before treatment and after 8 and 16 weeks of treatment.RESULTS: VAS and WOMAC scores significantly decreased and SF 36 scores significantly increased after treatment in both groups compared with before treatment(P < 0.05). There were significant differences in VAS, WOMAC, and SF 36 score changes between the two groups at week 16(P < 0.05).There was a significant increase in VAS and WOMAC scores in the control groups(P < 0.05) between weeks 8 and 16, but no significant difference was found in the treatment group(P > 0.05).CONCLUSION: Huqian Wan could effectively improve the clinical symptoms and quality of life in patients with KOA. It could also have a better and longer lasting curative effect without obvious adverse events compared with Votalin.

Therapeutic observation of 'warming-unblocking needling technique'for knee osteoarthritis due to deficiency of liver and kidney

Objective:To compare the clinical efficacy between'warming-unblocking needling technique7 and reinforcing method by twirling in treating knee osteoarthritis(KOA)due to deficiency of liver and kidney.Methods:A total of 72 patients with KOA were divided into a warming-unblocking group and a reinforcing group by complete randomized method,with 36 cases in each group.Xuehai(SP 10),Heding(EX-LE 2),Liangqiu(ST 34);Neixiyan(EX-LE 4),Xizhong(Extra),Dubi(ST 35),Yinlingquan(SP 9),Zusanli(ST 36)and Yanglingquan(GB 34)were the main points in both groups.Taichong(LR 3),Taixi(Kl 3)and Sanyinjiao(SP 6)were the combined points.'Warming-unblocking needling technique'was applied in the warming-unblocking group,and reinforcing method by twirling was applied in the reinforcing group.The treatments were performed once a day,10 times as a treatment course,at a 2-day interval between two courses,for 3 courses in total.The scores of Western Ontario and McMaster Universities osteoarthritis index(WOMAC)in the two groups were observed before and after the treatment,and the clinical efficacy was compared.Results:After treatment,the total effective rate was 94.3%in the warming-unblocking group and 87.9%in the reinforcing group,and the difference between the two groups was statistically significant(P<0.05).The component scores of pain,stiffness and daily activities in WOMAC and total WOMAC scores in both groups decreased after treatment(all P<0.01).The component and total WOMAC scores in the warming-unblocking group were lower than those in the reinforcing group(P<0.05 or P<0.01).Conclusion:'Warming-unblocking needling technique' has significant efficacy than reinforcing method by twirling in treating KOA due to deficiency of liver and kidney.It can significantly improve pain,stiffness and daily functional activities of patients.

独活桂辛方对腰椎间盘突出症(肝肾亏虚证)患者疼痛及腰椎功能的影响

目的观察独活桂辛方联合针刺、布洛芬缓释胶囊治疗腰椎间盘突出症(肝肾亏虚证)的临床疗效。方法将85例患者随机分为两组,观察组43例,予以独活桂辛方联合针刺、布洛芬缓释胶囊口服,对照组42例,予以针刺联合布洛芬缓释胶囊口服。疗程3周。结果观察组总有效率为92.68%,高于对照组的78.05%(P<0.05)。治疗后及随访时,两组患者中医症状积分、视觉模拟量表(VAS)评分、Roland-Morris功能障碍调查表(RMDQ)均较治疗前明显降低(P<0.05),观察组下降更明显(P<0.05);两组患者日本骨科协会腰椎(JOA)评分均较治疗前明显升高(P<0.05),观察组升高更明显(P<0.05)。治疗后,两组患者血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、超敏C反应蛋白(hs-CRP)水平均较治疗前明显下降,观察组下降更明显(P<0.05)。结论独活桂辛方联合针刺、布洛芬缓释胶囊可减轻腰椎间盘突出症(肝肾亏虚证)患者腰腿疼痛症状,降低血清IL-1β、IL-6、h-CRP水平,改善腰椎功能。

基于“肝-脑-目”轴探讨中医药论治干眼

“肝”“脑”“目”三者在生理及病理上相互联系,形成了“肝-脑-目”轴交互体系。其关系存在多向交互串扰,影响干眼的发生发展。应用脏腑辨证理论从生理和病理两个层面对“肝-脑-目”轴的内涵进行阐述,并提出“肝-脑-目”轴的3种失衡状态及其与干眼发病的关系,且肝、肾与目的关系为“肝-脑-目”轴理论之关键。提出清肝疏肝以畅“肝-脑-目”轴、益精生髓以充“肝-脑-目”轴、补肝肾阴以理“肝-脑-目”轴的中医药治疗干眼思路,以期为临床治疗干眼提供参考。

健脾疏肝熄风汤治疗脾虚肝亢型小儿多发性抽动症临床研究

目的:探讨健脾疏肝熄风汤治疗脾虚肝亢型小儿多发性抽动症(multiple tourette disease,MTD)的临床疗效。方法:将62例MTD患儿随机分为对照组和观察组,每组各31例。对照组予硫必利片治疗,观察组在此基础上予健脾疏肝熄风汤内服治疗,两组均连续治疗12周。治疗结束后,比较两组耶鲁抽动症整体严重程度量表(yale global tic severity scale,YGTSS)评分、脾虚肝亢证单项症状评分的变化情况,以及临床疗效、中医证候疗效和不良反应发生情况。结果:治疗后两组YGTSS评分、脾虚肝亢证单项症状评分均降低(P<0.05),且观察组降低程度大于对照组(P<0.05);临床总有效率观察组[96.77%(30/31)]高于对照组[74.19%(23/31)](P<0.05);中医证候疗效总有效率观察组[100.00%(31/31)]高于对照组[80.65%(25/31)](P<0.05);不良反应发生率观察组[9.68%(3/31)]低于对照组[22.58%(7/31)](P<0.05)。结论:健脾疏肝熄风汤治疗脾虚肝亢型小儿MTD疗效明显,能控制疾病发展,改善中医证候,降低不良反应发生率。

穴位注射红花黄色素联合四逆酸枣仁合方在首发中风后不寐(肝郁血虚型)患者中的应用效果

目的 研究穴位注射红花黄色素联合四逆酸枣仁合方在首发中风后不寐(肝郁血虚型)患者中的应用效果。方法 选取158例首发中风后不寐(肝郁血虚型)患者作为研究对象,将其随机分为常规组、观察组,每组各79例。在常规西药治疗的基础上,常规组行穴位注射红花黄色素治疗,观察组行穴位注射红花黄色素联合四逆酸枣仁合方治疗,两组均治疗4周。比较两组治疗4周后的临床疗效和治疗前、治疗4周后中医证候积分、匹兹堡睡眠质量指数(PSQI)、脑卒中专用生活质量(SS-QOL)量表评分、血清神经递质水平、血清核转录因子-κB(NF-κB)、促食欲素A水平,以及不良反应发生率。结果 治疗4周后,观察组临床总有效率高于常规组(P<0.05);观察组中医证候积分、PSQI和血清去甲肾上腺素、P物质、NF-κB、促食欲素A水平均低于常规组,SS-QOL量表评分和血清多巴胺、5-羟色胺水平高于常规组(均P<0.05);两组不良反应总发生率差异无统计学意义(P>0.05)。结论 穴位注射红花黄色素联合四逆酸枣仁合方治疗首发中风后不寐(肝郁血虚型)患者疗效显著,可有效地缓解临床症状,调节神经递质水平,促进病情恢复。

补肾疏肝宁心汤联合针刺治疗肾虚肝郁型绝经综合征

目的 探讨补肾疏肝宁心汤联合针刺疗法在治疗肾虚肝郁型绝经综合征中的临床效果。方法 选取2021年1月-2023年3月铜陵市中医院肾虚肝郁型绝经综合征患者60例作为研究对象,将患者随机分为观察组与对照组,2组患者各30例。观察组患者予补肾疏肝宁心汤联合针刺治疗,对照组患者予西医常规治疗。对治疗前后各个患者Kupperman症状评分、PSQI睡眠指数以及性激素水平的变化进行统计学分析。结果 治疗3个月时、停药3个月时,2组的Kupperman症状评分及PSQI睡眠指数均较治疗前下降,观察组的Kupperman症状评分及PSQI睡眠指数与对照组比较,差异具有统计学意义(P <0.05)。在治疗3个月以及停药3个月时各组患者性激素水平较治疗前均有改善(P <0.05)。对照组患者停药3个月后LH、FSH上升,E2下降;观察组患者各项指标相对更稳定,与对照组比较,差异具有统计学意义(P <0.05)。结论 补肾疏肝宁心汤联合针刺疗法能显著改善肾虚肝郁型绝经综合征患者的潮热出汗、失眠、焦虑等症状,改善绝经综合征患者的Kupperman症状评分及PSQI睡眠指数,提高生活质量,具有临床意义,值得推广使用。

《辅行诀》与《脏腑标本虚实寒热用药式》中肝木疾病临床表现及治疗用药的异同点分析

目的《辅行诀》与《脏腑标本虚实寒热用药式》均采用五脏虚实辨证作为理论架构,本文比较两者关于肝木疾病辨证用药的异同点,为“汤液经法图”的现代应用和理论融入提供依据。方法收集《辅行诀》与《脏腑标本虚实寒热用药式》关于肝木疾病辨证论治章节的临床症状表现和治疗药物信息,数据标准化后拆分成独立的语义概念进行比较,计算相符度。最终拆分成24个独立症状与《辅行诀》比较并计算相符度,用药药味依据“汤液经法图”五味补泻五脏规律进行定味和标记,并计算与补肝泻肝缓肝的“辛酸甘”味相符度。结果《脏腑标本虚实寒热用药式》肝木疾病包含24个症状,其中17个症状与《辅行诀》相符,相符度为70.8%,共有症状包括头痛、多怒、两胁肿痛等,《脏腑标本虚实寒热用药式》独有症状如卵缩、寒热虐等。同时,《脏腑标本虚实寒热用药式》包含76个肝木病治疗中药,其中辛味、酸味和甘味药总和占比为71.05%。结论《脏腑标本虚实寒热用药式》与《辅行诀》关于肝木疾病临床症状和治疗药物的认识有许多相同之处,相似度高达70%,两者可能具有相同的起源。五脏虚实+五味补泻辨证论治体系具有真实历史背景和临床实践价值。

Exploring pathogenesis in subjects with subjective Tinnitus having kidney deficiency pattern in terms of Traditional Chinese Medicine based on serum metabolic profiles

OBJECTIVE: To investigate the metabolic pathogenesis in subjects with subjective tinnitus(ST)having kidney deficiency pattern(KDP)(ST/KDP) in terms of Traditional Chinese Medicine.METHODS: Three groups of subjects, including healthy individuals, subjects with ST/KDP, and subjects who were healthy initially and then developed ST/KDP one year later(healthy → ST/KDP),were recruited for this study. Serum metabolic profiles of all subjects were analyzed using ultra-performance liquid chromatography coupled with quadruple-time-of-flight mass spectrometry. The metabolic characteristics of the ST/KDP subjects were determined, and the corresponding biomarkers were predicted. The metabolomics data from the healthy → ST/KDP subjects were collected for further verification.RESULTS: Twelve metabolites in the ST/KDP subjects were different from those of the healthy control subjects. Of these metabolites, according to the prediction, except for octanoic acid, other metabolites might characterize ST/KDP. Ten metabolites at the outcome ST/KDP stage were different from those at the initial(control) stage. Through the comparison of these metabolites with the predicted metabolites, five common metabolites, including upregulated glutamate, serotonin, oroticacid and 8-oxoguanine, as well as downregulated taurine, were found. These common metabolites were significantly associated with canonical pathways including calcium signaling, γ-aminobutyric acid(GABA) receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.CONCLUSION: The metabolic pathogenesis in ST/KDP subjects was characterized by upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, additionally, perturbations of calcium signaling, GABA receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.

热敏灸、热敏穴位贴敷联合独活寄生汤三联方案治疗膝骨性关节炎(肝肾亏虚证)的临床研究

目的探讨热敏灸、热敏穴位贴敷联合独活寄生汤三联方案治疗膝骨性关节炎(knee osteoarthritis,KOA)肝肾亏虚证患者的临床疗效和安全性。方法招募2022年5月至2023年6月江西中医药大学附属医院风湿病科、特色治疗部门诊及住院部确诊的KOA肝肾亏虚证患者84例,按随机数字表法分为对照组和观察组,每组42例。对照组采用传统艾灸+常规康复训练治疗,观察组采用热敏灸、热敏穴位贴敷联合独活寄生汤三联方案治疗。比较治疗前后数字评估量表(numerical rating scale,NRS)、西部安大略麦克马斯特大学骨关节炎指数可视化量表(Western Ontario and McMaster university osteoarthritis index visualization scale,WOMAC)、健康调查简表(the MOS item short from health survey,SF-36)的评分,以及基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)和透明质酸(hyaluronic acid,HA)的含量;记录治疗期间的不良反应,比较治疗后的临床疗效。结果试验期间有3例脱落,实际完成81例(对照组40例、观察组41例)。两组患者治疗后NRS、WOMAC评分均较治疗前明显下降(P<0.05);观察组治疗后NRS、WOMAC评分较对照组更低(P<0.05)。两组患者治疗后SF-36评分(躯体状况和精神状况)均较治疗前升高(P<0.05);观察组治疗后在躯体状况改善优于对照组(P<0.05),而精神状况改善程度两组差异无统计学意义(P>0.05)。与治疗前相比,两组患者在治疗后MMP-3含量均下降(P<0.05)、HA含量均升高(P<0.05);观察组治疗前后的变化情况明显比对照组更高(P<0.05)。观察组总有效率高于对照组(P<0.05),两组患者不良反应发生率差异无统计学意义(P>0.05)。结论热敏灸、热敏穴位贴敷结合独活寄生汤三联方案对KOA肝肾亏虚证患者治疗效果显著,能有效减轻疼痛程度,改善膝关节功能,调节血清和关节腔内炎症因子水平,有效缓解炎性病理状态,提高生活质量,值得临床推广应用。

辨证取穴温针灸联合揿针治疗肝肾亏虚型膝骨关节炎的临床研究

目的观察辨证取穴温针灸联合揿针治疗肝肾亏虚型膝骨关节炎(knee osteoarthritis,KOA)的临床疗效。方法将来自河北省唐山市第二医院的80例肝肾亏虚型KOA患者随机分为观察组和对照组,每组40例。对照组采用局部取穴温针灸治疗,观察组在对照组的基础上采用血海、梁丘、肝俞、肾俞穴温针灸配合揿针,每周(周一、周四)治疗2次,共治疗8周。观察两组患者治疗前后的中医证候评分、KOA功能(Lequesne)指数评分、视觉疼痛评分(visual analogue scale,VAS)、日常生活能力评价(Barthel评分)变化,ELISA法检测血清Toll样受体4(toll-like receptor,4,TLR4)、核因子κB(nuclear factorκB,NF-κB)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin 1β,IL-1β)、总抗氧化能力(total antioxidant capacity,TAOC)、过氧化脂质(lipid peroxide,LPO)水平变化,并比较临床疗效。结果(1)两组患者治疗后的各项中医证候评分均较治疗前下降(P<0.05),观察组治疗后的各项中医证候评分均较对照组下降(P<0.05)。(2)两组患者治疗后Lequesne指数评分均较治疗前下降(P<0.05),观察组治疗后Lequesne指数评分较对照组下降(P<0.05)。(3)两组患者治疗后的VAS评分均较治疗前下降(P<0.05),Barthel评分均较治疗前升高(P<0.05);观察组治疗后的VAS评分较对照组下降(P<0.05),Barthel评分较对照组升高(P<0.05)。(4)两组患者治疗后TLR4、NF-κB p65、TNF-α、IL-1β、TAOC、LPO水平均较治疗前下降(P<0.05),观察组治疗后上述指标均较对照组下降(P<0.05)。(5)对照组KOA患者临床总有效率为72.5%,观察组为85.0%,差异无统计学意义(P>0.05)。结论辨证取穴温针灸配合揿针治疗肝肾亏虚型KOA患者能够显著改善临床症状,降低血清炎症因子和氧化应激指标水平,且优于局部取穴温针灸治疗。

猫传染性腹膜炎的诊断与治疗研究进展

猫传染性腹膜炎(FIP)是由猫传染性腹膜炎病毒(FIPV)引起的高致死性疾病。目前,FIP确诊依然存在较大困难,西兽医诊断该病需将问诊、临床症状观察、实验室检查等结果进行综合分析,从而尽可能提高患猫生前诊断的准确性。在治疗方面主要采用抗病毒治疗和对症治疗。目前国外临床上抗FIPV的药物主要为蛋白酶抑制剂和核苷类似物两大类,国内尚无合规且有效的治疗药物。根据中兽医理论,依据八纲辨证和脏腑辨证对FIP的病例进行初步分型,可将其分为气虚伤脾、湿热壅盛和肝肾阴亏3种证型。近年来利用中药复方制剂治疗或辅助治疗FIP效果良好,能够弥补西兽医当下诊治该病的不足。总结归纳西兽医和中兽医对FIP的诊断和治疗现状,以期为该病的临床诊疗提供参考。

壮医药线点灸治疗肝肾亏虚型膝骨关节炎的疗效观察及对血清TGF-β1、OPG、5-HT水平及Th1/Th2细胞因子平衡的影响

目的 观察壮医药线点灸治疗肝肾亏虚型膝骨关节炎的临床疗效及对血清转化生长因子-β1(transforming growth factor-β, TGF-β1)、护骨素(osteoprotegerin, OPG)、5-羟色胺(5-hydroxytryptamine,5-HT)水平及Th1/Th2细胞因子平衡的影响。方法 将116例肝肾亏虚型膝骨关节炎患者随机分为观察组和对照组,每组58例。对照组采用西医常规治疗,观察组在对照组治疗基础上联合壮医药线点灸治疗。比较两组治疗前后血清TGF-β1、环氧合酶-2(cyclooxygenase-2, COX-2)、OPG、白介素-6(interleukin-6, IL-6)、白介素-10(interleukin-10, IL-10)、5-HT和前列腺素E2(Prostaglandin E2, PGE2)水平,观察两组治疗前后健康调查简表(36-item short-form health survey, SF-36)、视觉模拟量表(visual analog scale, VAS)评分和西安大略和麦克马斯特大学骨关节炎指数(Western Ontario and Mc Master Universities osteoarthritis index, WOMAC)评分变化,并比较两组临床疗效。结果 治疗后,两组血清TGF-β1、COX-2、IL-6、PGE2和5-HT水平较治疗前降低(P<0.05),血清OPG、IL-10水平较治疗前升高(P<0.05);观察组血清TGF-β1、COX-2、IL-6、PGE2和5-HT水平高于对照组(P<0.05),血清OPG、IL-10水平低于对照组(P<0.05)。治疗后,两组VAS评分、WOMAC评分较治疗前降低(P<0.05),SF-36评分较治疗前升高(P<0.05);观察组VAS评分、WOMAC评分低于对照组(P<0.05),SF-36评分高于对照组(P<0.05)。观察组总有效率为94.8%,高于对照组的79.3%(P<0.05)。结论 在西医常规治疗基础上,采用壮医药线点灸治疗肝肾亏虚型膝骨关节炎效果显著,可减轻炎症反应,促进Th1/Th2平衡恢复,调节骨质代谢。

Evaluation of a granulated formula for the nerve root type and vertebral artery type of cervical spondylosis：a multicenter,single-blind,randomized,controlled,phase Ⅲ clinical trial

OBJECTIVE:To evaluate the effectiveness and safety of cervical spondylosis formula granules in reducing the symptoms of patients with the nerve root type and the vertebral artery type of cervical spondylosis.METHODS:This was a multicenter, single-blind,randomized, controlled trial.From April 2002 to November 2003, 499 patients were randomly assigned to either the treatment or the control group.The treatment group was orally administered granules prepared with a formula for cervical spondylosis, while the control group was given Jingfukang granules.The treatment course was 1 month for both groups.RESULTS:In patients with the nerve root type of cervical spondylosis, the total effect rate in the treatment group(87.21%) was significantly higher than that in the control group(80.70%, P < 0.01).After the treatment period in both groups, the treatment group had a significantly greater rate of resolution of pain, numbness of the upper limbs, muscle strength of the upper limbs, and fatigue than the control group(all P < 0.05).In patients with the vertebral artery type of cervical spondylosis, the total effect rate in the treatment group(82.07%) was similar to that in the control group(71.21%, P >0.05).After the treatment period in both groups,the treatment group had a significantly greater rate of resolution of weakness of the waist and knees than the control group(P < 0.05).CONCLUSION:The cervical spondylosis formula granules significantly improve numbness, muscle strength, and fatigue, and reduce pain in patients with the nerve root type of cervical spondylosis,and improve the weakness of the waist and knees in patients with the vertebral artery type of cervical spondylosis.

Comparative observation of the effect of electroacupuncture combined with heat-sensitive moxibustion and western medicine for premature ovarian failure

Objective To compare the clinical efficacy between electroacupuncture combined with heat-sensitive moxibustion and western medicine for treatment of premature ovarian failure. Methods Eighty patients were randomly divided into an electroacupuncture combined with heat-sensitive moxibustion group（group A, n=40） and a western medicine group（group B, n=40）. Zǐgōng（子宫 EX-CA 1）, Xuèhǎi（血海 SP 10）, Gānshū（肝俞 BL 18） and Shènshū（肾俞 BL 23） were selected in group A, and acupuncture combined with heat-sensitive moxibustion were carried out. Climen was taken orally in group B. The score changes of menstrual cycle, tidal fever and sweatiness, emotional excitement, soreness and weakness of waist and knees, dizziness and tinnitus, as well as the cured and markedly effective rate of the patients in the two groups before and after two courses of treatment were compared. Results The cured and markedly effective rate of group A was 72.5%（29/40）, which was superior to that of group B（37.5%, 15/40）（ P〈0.05）. The symptom scores were improved significantly in the two groups after treatment（all P〈0.05）, and the improvement in group A was superior to that in group B（all P〈0.05）. Conclusion The efficacy of electroacupuncture combined with heat-sensitive moxibustion is superior to that of conventional western medicine in treatment of premature ovarian failure.

Curative effect of assisted reproduction technology by Traditional Chinese Medicine multi-channel interventional therapy on 95 cases of in vitro fertilization and embryo transfer failure

OBJECTIVE: To evaluate the curative effect of Traditional Chinese Medicine(TCM) multi-channel interventional therapy on women with Assisted Reproductive Technology(ART) failure, to compare the curative effect of the dual therapy and triple therapy on women with ART Failure, and to choose the best TCM interventional therapeutic plan.METHODS: The 95 cases with ART Failure from West China second University Hospital of Sichuan University meeting the inclusion criteria were randomly divided into three groups:dual therapy group(31 cases), triple therapy group(33 cases)and control group(31 cases). According to the intervene treatment of in vitro Fertilization and Embryo Transfer(IVF-ET) long cycle scheme, the control group wait naturally for 3 months before IVFET. The dual therapy group take TCM prescriptionⅡ of cultivating emotion and assisting reproduction and auricular acupoint therapy for 3 months before IVF-ET, then Western Medicine treatment progestin supporting as well as auricular application and Antai Recipe after IVF-ET transplantation.The triple therapy group take TCM prescription Ⅱof cultivated emotion and assisted reproduction,auricular acupoint therapy and retention enema of TCM, and combination treatment the same as dual therapy group after transplantation. The natural pregnancy number, the period condition of secondary IVF-ET and the improvement of the kidney deficiency, liver depression and blood stasis syndrome among those three groups were compared.RESULTS: It was showed from analysis in 95 cases with ART failure that the number of natural preg-nancy was as followings: 3 patients from the dual therapy group, 10 patients from the triple therapy group, and no patient from the control group. The comparison among three groups have statistical significance(P < 0.05). The treatment group is superior to the control group, while the triple therapy is superior to the dual therapy. The comparison of the condition of the fertility rate, clinical pregnancy rate, biochemical pregnancy rate and early abortion rate during the period of secondary IVF-ET between pre-therapy and post-treatment of both the dual therapy group and the triple therapy group have statistical significance(P < 0.05). The comparison of the improvement of the kidney deficiency,liver depression and blood stasis syndrome between pre-therapy and post-treatment of both the dual therapy group and the triple therapy group have statistical significance(P < 0.05). The comparison between three groups after treatment have statistical significance(P < 0.05).CONCLUSION: TCM multi-channel interventional therapy can increase the natural pregnancy rate of patients with ART Failure(the triple therapy is superior to the dual therapy); it can increase the fertility rate, clinical pregnancy rate, and decrease the early abortion rate during the period of secondary IVF-ET; it can improve syndromes of kidney deficiency, liver depression and blood stasis.

Effect of traditional Chinese comprehensive therapy on gestation in patients with previously failed in vitro fertilization and embryo transfer

OBJECTIVE: To explore the effect of traditional Chinese comprehensive therapy(TCCT) on promoting gestation in patients with previously failed in vitro fertilization and embryo transfer(IVF-ET) because of kidney deficiency, liver stagnation, and blood stasis(KLB).METHODS: Sixty-seven patients were enrolled in this study and divided into two groups: a trial group with 35 patients and a control group with 32 patients. The trial group was given TCCT for 3months, then administered IVF-ET or awaited natural pregnancy. The control group was administered IVF-ET without TCCT 3 months after the previous IVF-ET or natural pregnancy attempt. The patterns of KLB were observed both before and after treatment. The natural pregnancy rate of the two groups was calculated after treatment. Differences between the two groups were compared after using IVF-ET treatment again in fertilized egg number and fertilization rate were evaluated.RESULTS: After treatment with TCM comprehensive therapy, seven patients in the treatment group became pregnant, while there were no successful conceptions in the control group. The difference in clinical pregnancy rate in the initial cycle and transfer cycle of IVF were significantly different(P <0.05). The trial group had a significantly higher conception rate than that of the control group(P <0.05).CONCLUSION: TCCT can promote the natural pregnancy rate in patients with previously failed IVF-ET.TCCT could increase patients' fertilized egg number, fertilization rate, pregnancy rate, and clinical pregnancy rate after another IVF-ET treatment.TCCT can also relieve the symptoms of KLB.

慢性萎缩性胃炎大鼠胃黏膜细胞凋亡与中医证型的相关性

目的探讨大鼠不同证型慢性萎缩性胃炎(CAG)证候发生时胃黏膜细胞凋亡及调控蛋白表达水平的变化,从细胞凋亡的角度揭示CAG各证候的实质。方法复制大鼠脾虚证、肝郁证和湿热证CAG病证结合模型,检测胃黏膜上皮细胞凋亡水平和胃黏膜细胞凋亡调控蛋白Bcl-2、Fas抗原的表达水平。结果各模型组大鼠胃黏膜细胞凋亡指数均显著高于正常对照组,各证候模型组中以脾虚CAG组最高。各模型组大鼠胃黏膜Bcl-2、Fas蛋白表达量均显著高于正常对照组,各证候模型组中以脾虚CAG组最高。结论CAG大鼠胃黏膜细胞凋亡指数、Bcl-2、Fas蛋白表达与CAG证候有一定的相关性,以脾虚CAG组大鼠胃黏膜细胞凋亡指数最高和Bcl-2、Fas表达量最高。