Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats

Delonix elata L.is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases,liver diseases and rheumatic problems.However,systematic evaluation of its wound healing activity is lacking.Thus,in the present study,we aimed to assess the wound healing activity of D.elata stem bark extract(DSE) and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside(QRPG) in rats.The formulations effects on wound healing were assessed by the wound contraction rate,epithelialization period,tensile strength,content of the hydroxyproline,hexosamine and uronic acid in granulation tissue,histopathological studies and Col 1 α(I) expression level in wound tissue by reverse transcription polymerase chain reaction(RT-PCR) study.The topical application of DSE ointment caused faster epithelialization,significant wound contraction(100%),and better tensile strength(710.5 ± 10.5 g/cm^2),while QRPG showed wound epithelialization with 98.2%contraction,better than that of the control group(78.18%).The biochemical analysis of granulation tissue revealed that DSE and QRPG significantly increased hydroxyproline,hexosamine and uronic acid content.A significant increase in the expression of Col 1 α(I) was observed in the wound tissue of DSE and QRPG treated rats.DSE and QRPG were shown to enhance wound healing by increasing collagen synthesis through upregulation of Col 1 α(I),thus validating ethnomedicinal uses.

Adulticidal efficacy of Delonix elata against filariasis vector mosquito,Culex quinquefasciatus(Diptera:Culicidae)

Objective:To evaluate the adulticidal activity and toxicity of different solvent crude extracts of Delonix elata(D.elata)against filariasis vector mosquito Culex quinquefasciatus(Cx.quinquefasciatus).Methods:The adulticidal activities of crude hexane,benzene,ethyl acetate,chloroform and methanol leaf and seed extracts of D.elata were assayed for their toxicity against vector mosquitoCx.quinquefasciatus.Bioassay was carried out by WHO method for determination of adulticidal activity against mosquitoes.The adult mortality was observed after 24 h of exposure.Results:All extracts showed moderate adulticidal effects;however,the highest adult mortality was found in methanol extract of D.elata leaf against the adults of Cx.quinquefasciatus with the LC_(50) and LC_(90) values at 197.28 and 347.45mg/L,respectively.Conclusions:These results suggest that the leaf solvent plant extracts have the potential to be used as an ideal eco-friendly approach for the control of mosquitoes.This study provides first report on the mosquito adulticidal activity of D.elata plant extracts against filariasis vector mosquito,Cx.quinquefasciatus.

Mosquito adulticidal properties of Delonix elata(Family:Fabaceae)against dengue vector,Aedes aegypti(Diptera:Culicidae)

Objective:To determine the adulticidal activity of hexane,benzene,chloroform,ethyl acetate and methanol leaf and seed extracts of Delonix elata(D.elata)against Aedes aegypti(Ae.aegypti).Methods:The bioassay was conducted in an experimental kit consisting of two cylindrical plastic tubes both measuring 125 mm×44 mm following the WHO method;mortality of the mosquitoes was recorded after 24 h.Results:The adulticidal activity of plant leaf and seed extracts showed moderate toxic effect on the adult mosquitoes after 24 h of exposure period.However,the highest adulticidal activity was observed in the leaf methanol extract of D.elata against Ae.aegypti with the LC_(50) and LC_(90) values 162.87 and 309.32 mg/L,respectively.Conclusions:From this result,it can be concluded the crude extract of D.elata was an excellent potential for controlling Ae.aegypti mosquitoes.