The rapid cooling of a metallic liquid(ML)results in short-range order(SRO)among the atomic arrangements and a disordered structure in the resulting metallic glass(MG).These phenomena cause various possible features i...The rapid cooling of a metallic liquid(ML)results in short-range order(SRO)among the atomic arrangements and a disordered structure in the resulting metallic glass(MG).These phenomena cause various possible features in the microscopic structure of the MG,presenting a puzzle about the nature of the MGs’microscopic structure beyond SRO.In this study,the nanoscale density gradient(NDG)originating from a sequential arrangement of clusters with different atomic packing densities(APDs),representing the medium-range structural heterogeneity in Zr_(60)Cu_(30)Al_(10)MG,was characterized using electron tomography(ET)combined with image simulations based on structure modeling.The coarse polyhedrons with distinct facets identified in the three-dimensional images coincide with icosahedron-like clusters and represent the spatial positions of clusters with high APDs.Rearrangements of the different clusters according to descending APD order in the glass-forming process are responsible for the NDG that stabilizes both the supercooled ML and the amorphous states and acts as a hidden rule in the transition from ML to MG.展开更多
Aim: To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSper...Aim: To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate. Methods: This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up method. These specimens were then cryopreserved. Thirteen specimens were processed by both PureSperm (40 % and 80 %) and Isolate (50 % and 90 %) double density gradient techniques. The two fractions processed by both PureSperm and swim-up were analyzed for post-wash sperm characteristics. Post-thaw analysis was done after 24 hours. Sperm fractions obtained after processing with PureSperm and ISolate were compared for post-wash sperm characteristics and ROS levels. Results: Specimens prepared with PureSperm had significantly higher median total motile sperm counts (TMSC) (32.2 x 10~6 vs. 17.6 x 10~6), recovery rates (69.2 % vs. 50.0 %), and longevity at 4 hours (83.0 % vs. 55.0 %) compared to specimen prepared by swim-up. Post-thaw specimens also had a higher recovery and longevity at 4 hours with PureSperm as compared to the swim-up. Semen specimens processed by PureSperm had significantly higher total sperm count, TMSC, and percentage recovery rates (30.0 % vs. 19.7 %) than ISolate. Conclusion: Semen quality is better preserved in fresh and cryopreserved semen prepared with PureSperm density gradient compared to swim-up. A significant enrichment of sperm is observed with PureSperm compared to ISolate. Higher recovery rates of mature motile sperm obtained after PureSperm sperm preparation may be beneficial for successful ART. i展开更多
We modeled the effect of the deformation of a Density Gradient Zone (DGZ) on a local gravity field using a cubical model and introduced a new method to simulate a complex DGZ (CDGZ). Then, we analyzed the features...We modeled the effect of the deformation of a Density Gradient Zone (DGZ) on a local gravity field using a cubical model and introduced a new method to simulate a complex DGZ (CDGZ). Then, we analyzed the features of the model for the influence of the deformation of the DGZ on the local gravity field. We concluded that land-based gravity is not sensitive to the thickness of the DGZ and that the magnitude of the contribution of the DGZ is one order less than that of the volume strain with the same displacement.展开更多
A general solution of the electrostatic potential that determines the maximum light-ion energy is derived for the test-particle acceleration model by taking into account the influence of the substrate-ion density grad...A general solution of the electrostatic potential that determines the maximum light-ion energy is derived for the test-particle acceleration model by taking into account the influence of the substrate-ion density gradient. It is shown that the substrate-ion density structure is also dependent on laser pulse duration. In the picosecond or sub-picosecond regime, the decreasing density gradient of the substrate-ions leads to an evident reduction in the acceleration efficiency of the light-ions. However, this kind of influence is negligible in the ultrashort regime.展开更多
Objective:Sperm preparation techniques and cryopreservation are widely used in assisted reproductive techniques(ART).How to improve the quality of sperm management is a matter of great concern.Phospholipase C-zeta(PLC...Objective:Sperm preparation techniques and cryopreservation are widely used in assisted reproductive techniques(ART).How to improve the quality of sperm management is a matter of great concern.Phospholipase C-zeta(PLCζ)is considered a sperm-specific agent that activates oocyte activation and thus playing a crucial role in male fertility.However,the potential mechanisms by which semen processing and cryopreservation on PLCζcontribute to keyhole have not been addressed.Methods:In this study,semen samples were taken from have not been addressed 10 normozoospermic men.Each semen sample was assigned to the following groups:density gradient centrifugation(DGC)as control,microfluidic sorting,and cryopreservation.Sperm parameters of molity,viability,membrane integrity,and intracellular ROS were evaluated during sperm preparation and cryopreservation.The expression of PLCζin human sperm was determined by immunofluorescence and western blotting.Results:The results showed that molity,viability,and membrane integrity decreased in cryopreservation group.Intracellular ROS were also significantly increased compared to the the control group.There was no significant difference between DGC and microfluidic sorting group.Our investigation revealed that total levels of PLCζwere comparable between DGC and microfluidic sorting,but there were significantly reduced levels of PLCζafter cryopreservation as quantified by both immunofluorescenceand immunoblotting.PLCζimmunofluorescence in sperm revealed different PLCζlocalization patterns around the acrosomal(Ac),equatorial(Eq),post-acrosomal(PA)areas of sperm heads,and their combination.The predominant patterns of PLCζlocalization in DGC were similar to that of microfluidic sorting,with strong,with staining.In contrast,PLCζstaining in freeze-thawed sperm was considerably weaker fluorescence intensity.Conclusion:This study clarified the mechanism of sperm preparation and cryopreservation underlying effect on sperm characteristic,accompanied with PLCζexpresion.We demonstrated that microfluidic sorting provides a highly efficient preparation method for clinical selection of PLCζ-expressing sperm comparable to DGC gene expression.It is suggested that the cryopreservation of sperm has a significant detrimental effect on PLCζ.展开更多
Based on the dynamic shock response of the material and structure,the hypervelocity impact processes and mechanisms of long composite rods with axial density/impedance gradients penetration into fourlayer targets were...Based on the dynamic shock response of the material and structure,the hypervelocity impact processes and mechanisms of long composite rods with axial density/impedance gradients penetration into fourlayer targets were studied through experiments and numerical simulation methods.The propagation law of the shock waves,together with the structural responses of the projectiles and targets,the formation and evolution of the fragment groups formed during the processes and their distributions were described.The damage of each target plate was quantitatively analysed by comparing the results of the experiment and numerical simulation.The results showed that the axial density/impedance gradient projectiles could decrease the impact pressure to a certain extent,and the degree of damage to the target plate decreased layer by layer when the head density/impedance of the projectile was high.When the head density/impedance of the projectile was low,the degree of target damage first increased layer by layer until the projectile was completely eroded and then it decreased.The results can provide a reference for the design and application of long rods with axial composite structure for velocities ranging from 6 to 10 Ma or greater.展开更多
Objective:The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocy...Objective:The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocytes by intra-cytoplasmic sperm injection(ICSI)following density gradient centrifugation(DGC)and swim-up(SU)procedures.Methods:Semen samples were collected from 30 outpatients who visited the Center for Reproductive Medicine for semen analyses.Following sperm selection by DGC and SU procedures,the liquified semen samples were divided into three groups and incubated at 4,25,and 37°C,respectively.Following incubation for 24,48,and 72 hours,the sperm motility and sperm DNA fragmentation index(DFI)were analyzed.Results:Following the combination of DGC and SU procedures,the sperm motility(91.8%±8.6%vs.50.8%±13.1%)and DFI(5.1%±7.9%vs.13.0%±11.6%)were significantly improved(P<0.01)compared to those without any treatment.The sperm motility of the 3 groups significantly declined(P<0.05)post-incubation compared to that of the groups prior incubation.However,sperm motility significantly increased(76.9%±10.4%)(P<0.05)at 25°C compared to that of the other 2 groups(53.5%±11.0%and 47.6%±10.2%).Sperm DFI significantly increased(P<0.05)at 37°C following incubation for 24 and 72 hours in comparison to that of the other 2 groups.However,the sperm DFI did not significantly increase when the sperm samples were incubated at 4(5.7%±5.9%)and 25°C(6.8%±5.6%)for 24 hours compared to that before incubation(5.1%±7.9%).Conclusions:These results indicate that the sperm quality,in terms of motility and DFI,can be efficiently improved by DGC in combination with SU.Following which,the sperm samples can be incubated at 25°C and be used on the second day for insemination of in vitro matured oocytes by ICSI.展开更多
A three-dimensional density model of the crust and uppermost mantle is determined by the inversion of a set of GOCE gravity and gradients residual anomalies beneath the eastern margin of the Tibetan Plateau and its su...A three-dimensional density model of the crust and uppermost mantle is determined by the inversion of a set of GOCE gravity and gradients residual anomalies beneath the eastern margin of the Tibetan Plateau and its surrounding areas. In our work, we choose five independent gravity gradients (Txx, Tzz, Txy, Txz, Tyz) to perform density inversion. Objective function is given based on Tikhonov regularization theory. Seismic S-wave velocities play the role of initial constraint for the inversion based on a relationship between density and S-wave velocity. Damped Least Square method is used during the inversion. The final density results offer some insights into understanding the underlying geodynamic processes: (1) Low densities in the margin of the Tibet, along with low wave velocity and resistivity results, yield conversions from soft and weak Tibet to the hard and rigid cratons. (2)The lowest densities are found in the boundary of the plateau, instead of the whole Tibet indicates that the effects of extrusion stress environment in the margin affect the changes of the substance there. The substances and environments conditioning for the earthquake preparations and strong deformation in this transitional zone. (3) Evident low-D anomaly in the upper and middle crust in the Lasha terrane and Songpan-Ganzi terrane illustrated the eastward sub-ducted of southeastern Tibet, which could be accounts for the frequent volcano and earthouakes there.展开更多
The effect of the radial density configuration in terms of width, edge gradient and volume gradient on the wave field and energy flow in an axially uniform helicon plasma is studied in detail. A three-parameter functi...The effect of the radial density configuration in terms of width, edge gradient and volume gradient on the wave field and energy flow in an axially uniform helicon plasma is studied in detail. A three-parameter function is employed to describe the density, covering uniform,parabolic, linear and Gaussian profiles. It finds that the fraction of power deposition near the plasma edge increases with density width and edge gradient, and decays in exponential and "bumpon-tail" profiles, respectively, away from the surface. The existence of a positive second-order derivative in the volume density configuration promotes the power deposition near the plasma core, which to our best knowledge has not been pointed out before. The transverse structures of wave field and current density remain almost the same during the variation of density width and gradient, confirming the robustness of the m=1 mode observed previously. However, the structure of the electric wave field changes significantly from a uniform density configuration, for which the coupling between the Trivelpiece-Gould(TG) mode and the helicon mode is very strong, to non-uniform ones. The energy flow in the cross section of helicon plasma is presented for the first time, and behaves sensitive to the density width and edge gradient but insensitive to the volume gradient. Interestingly, the radial distribution of power deposition resembles the radial profile of the axial component of current density, suggesting the control of the power deposition profile in the experiment by particularly designing the antenna geometry to excite a required axial current distribution.展开更多
Density-graded cellular materials have tremendous potential in structural applications where impact resistance is required.Cellular materials subjected to high impact loading result in a compaction type deformation,us...Density-graded cellular materials have tremendous potential in structural applications where impact resistance is required.Cellular materials subjected to high impact loading result in a compaction type deformation,usually modeled using continuum-based shock theory.The resulting governing differential equation of the shock model is nonlinear,and the density gradient further complicates the problem.Earlier studies have employed numerical methods to obtain the solution.In this study,an analytical closed-form solution is proposed to predict the response of density-graded cellular materials subjected to a rigid body impact.Solutions for the velocity of the impinging rigid body mass,energy absorption capacity of the cellular material,and the incident stress are obtained for a single shock propagation.The results obtained are in excellent agreement with the existing numerical solutions found in the literature.The proposed analytical solution can be potentially used for parametric studies and for effectively designing graded structures to mitigate impact.展开更多
Critical properties of metallic materials,such as the yield stress,corrosion resistance and ductility depend on the microstructure and its grain size and size distribution.Solute atoms that favorably segregate to grai...Critical properties of metallic materials,such as the yield stress,corrosion resistance and ductility depend on the microstructure and its grain size and size distribution.Solute atoms that favorably segregate to grain boundaries produce a pinning atmosphere that exerts a drag pressure on the boundary motion,which strongly affects the grain growth behavior during annealing.In the current work,the characteristics of grain growth in an annealed Mg-1 wt.%Mn-1 wt.%Nd magnesium alloy were investigated by advanced experimental and modeling techniques.Systematic quasi in-situ orientation mappings with a scanning electron microscope were performed to track the evolution of local and global microstructural characteristics as a function of annealing time.Solute segregation at targeted grain boundaries was measured using three-dimensional atom probe tomography.Level-set computer simulations were carried with different setups of driving forces to explore their contribution to the microstructure development with and without solute drag.The results showed that the favorable growth advantage for some grains leading to a transient stage of abnormal grain growth is controlled by several drivers with varying importance at different stages of annealing.For longer annealing times,residual dislocation density gradients between large and smaller grains are no longer important,which leads to microstructure stability due to predominant solute drag.Local fluctuations in residual dislocation energy and solute concentration near grain boundaries cause different boundary segments to migrate at different rates,which affects the average growth rate of large grains and their evolved shape.展开更多
Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A tot...Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A total of 153 infertile men were involved. Conventional semen parameters and sperm chromatin structure assay (SCSA) parameters, that is, DNA fragmentation index (%DFI) and high DNA stainability (%HDS), were assessed on the flesh ejaculated semen samples, which were treated and incubated under different conditions. Negative correlations were identified between the %DFI and sperm concentration, motility, progressive motility and morphology. A lower percentage of DFI was detected in spermatozoa when density gradient centrifugation (DGC) was followed by swimup treatment in comparison with DGC alone (P 〈 0.01). Although the %DFI increased in a time-dependent manner with incubation both at room temperature (RT) and at 37℃ in air, the %DFI after 24 h at RT was significantly lower than that at 37℃ (P 〈 0.05). Incubation with 5% CO2 was effective in maintaining sperm motility (P 〈 0.01); however, it induced further elevation of %DFI (P 〈 0.001). Thus, sperm DNA damage was associated with longer incubation periods. Interestingly, common culture conditions, such as maintaining pH and temperature, compromised the sperm DNA integrity.展开更多
The hybrid finite analytic(HFA) method is a kind of numerical scheme in rectangular element. In order to simulate the shallow circulation in irregular bathymetry by HFA scheme, the model in sigma coordinate system was...The hybrid finite analytic(HFA) method is a kind of numerical scheme in rectangular element. In order to simulate the shallow circulation in irregular bathymetry by HFA scheme, the model in sigma coordinate system was obtained. The model has been tested against three cases: 1) Wind induced circulation; 2) Density driven circulation and 3) Seiche oscillation. The results obtained in the present study compare well with those obtained from the corresponding analytical solutions under idealized for the above three cases. The hybrid finite analytic method and the circulation model in sigma coordinate system can be used calculate the flow and water quality in estuaries and coastal waters.展开更多
Engineering-oriented simulations of quantum mechanical tunneling are often based on density-gradient (DG) theory. This paper presents an analytical solution to the DG equation for quantum tunneling through an ultra-...Engineering-oriented simulations of quantum mechanical tunneling are often based on density-gradient (DG) theory. This paper presents an analytical solution to the DG equation for quantum tunneling through an ultra-thin oxide in a MOS capacitor with an n+ poly-silicon gate obtained using the method of matched asymptotic expansions. Tunneling boundary conditions extend the approximation into the entire region of the poty-silicon gate, oxide barrier, and substrate. An analytical solution in the form of an asymptotic series is obtained in each region by treating each part of the domain as a separate singular perturbation problem. The solutions are then combined through 'matching' to obtain an approximate solution for the whole domain. Analytical formulae are given for the electrostatic potential and the electron density profiles. The results capture the features of the quantum effects which are quite different from classical physics pre- dictions. The analytical results compare well with exact numerical solutions over a broad range of voltages and different oxide thicknesses. The analytical results predict the enhancement of the quantum tunneling effect as the oxide thickness is reduced.展开更多
The influence of a molybdenum dust buildup on plasma edge turbulence has been studied in the EAST tokamak.The motion of the dust from the upper divertor region is detected by a fast visible CCD camera,the XUV spectrom...The influence of a molybdenum dust buildup on plasma edge turbulence has been studied in the EAST tokamak.The motion of the dust from the upper divertor region is detected by a fast visible CCD camera,the XUV spectrometer arrays,and the EUV spectrometer.The MoXV emission intensity sharply increases compared with the spectral lines of various ionization states of other elements,which implies that the dust particles are the molybdenum impurities.The radial distribution of Mo^(14+)ion simulated by a simplified 1 D transport model indicates that the molybdenum dust mainly deposits in the pedestal bottom region.Moreover,it is observed that the coherent mode(CM)appears atρ=0.94 after the molybdenum impurities enter the main plasma region.The influx of molybdenum impurities results in increasing pedestal electron density and decreasing pedestal electron temperature in contrast to that before the event of impurities dropping.It is also found that the electron density gradient in the pedestal increases when the ablation of the molybdenum impurities is observed in the pedestal region.The qualitative experimental results indicate that the onset of CM is likely related to the increase of the density gradient and edge collisionality in the pedestal.In comparison to the density gradient,the enhancement of CM amplitude largely depends on the increase of the edge collisionality.展开更多
Background: Density gradient centrifugation was reported as a technique of semen preparation in assisted reproductive techniques in humans and animals. This technique was found to be efficient in improving semen qual...Background: Density gradient centrifugation was reported as a technique of semen preparation in assisted reproductive techniques in humans and animals. This technique was found to be efficient in improving semen quality after harmful techniques such as cryopreservation. Recently a modified technique, single layer centrifugation,was proposed as a technique providing a large amount of high quality spermatozoa, and this treatment was performed before conservation. Single layer centrifugation has been studied prevalently in stallions and in boars,but limited data were available for bulls. Occasionally bulls are known to experience a transient reduction in semen quality, thus techniques that allow improvement in semen quality could be applied in this context. The aim of this study was the evaluation of single layer and double layer centrifugation by the use of iodixanol, compared with conventional centrifugation and non-centrifuged semen, on the sperm characteristics during the cryopreservation process in bulls with normal and poor semen quality.Results: Single layer centrifugation and double layer centrifugation both significantly increased the percentage of normal spermatozoa and decreased the percentage of non-sperm cells in poor quality samples, while both were ineffective in those of normal quality. Sperm characteristics in poor quality samples increased after single layer centrifugation and double layer centrifugation, reaching values similar to those recorded in normal samples, and this trend is maintained after equilibration and after cryopreservation. On the other hand, SLC and DLC resulted in a consistent reduction in the spermatozoa recovered, and this resulted in a reduction of the absolute amount of spermatozoa cryopreserved in the normal samples, without a clear improvement in sperm characteristics in this type of sample.Conclusions: These data suggested that both SLC and DLC could be performed in practice, but their application should be limited to the cases in which the quality of the spermatozoa recovered is more important than the total amount of spermatozoa.展开更多
Testicular sperm extraction is a common procedure used to find spermatogenic cells in men with nonobstructive azoospermia. The laboratory processing of biopsied testicular tissues needs to be performed meticulously to...Testicular sperm extraction is a common procedure used to find spermatogenic cells in men with nonobstructive azoospermia. The laboratory processing of biopsied testicular tissues needs to be performed meticulously to acquire a high yield of cells. In this study, the effectiveness of mincing the tissues after testicular biopsy was assessed using histological evaluation, as was the possible adverse effect of residual tissue on the migration of spermatogenic cells during density gradient centrifugation. Our results indicate that testicular residual tissue, when laid on the density gradient medium along with the sperm wash, hinders the spermatogenic cells' forming a pellet during centrifugation, and therefore impairs the intracytoplasmic sperm injection procedure. Whereas the mean number of recovered cells from the sperm wash medium (SWM) with residual tissue is 39.435 ~ 24.849, it was notably higher (60.189 ~ 28.214 cells) in the SWM without minced tissues. The remaining tissue contained no functional seminiferous tubules or spermatogenic cells in histological sections. In conclusion, the remaining residual tissue after mincing biopsied testicular tissue does not add any functional or cellular contribution to spermatogenic cell retrieval; in fact, it may block the cellular elements in the accompanying cell suspension from migrating through the gradient layers to form a pellet during centrifugation and cause loss of spermatogenic cells.展开更多
The impurity iron in silicon material will seriously affect the photoelectric conversion efficiency of silicon solar cells.However,the traditional silicon purification method has the disadvantages of long cycle,high e...The impurity iron in silicon material will seriously affect the photoelectric conversion efficiency of silicon solar cells.However,the traditional silicon purification method has the disadvantages of long cycle,high energy consumption and serious pollution.In this study,an efficient and green pulsed electric current purification technology is proposed.The electromigration effect of iron elements,the current density gradient driving of iron phase,and the gravity of iron phase all affect the migration behavior of iron phase in silicon melt under pulsed electric current.Regardless of the depth of electrode insertion into the silicon melt,the solubility of iron in silicon decreases under the pulsed electric current,which helps to form the iron phase.At the same time,the iron phase tends to sink toward the bottom under the influence of gravity.When the electrode is shallowly inserted,a non-uniform electric field is formed in the silicon melt,and the iron phase is mainly driven by the current density gradient to accelerate sink toward the bottom.When the electrode is fully inserted,an approximately uniform electric field is formed in the silicon melt,and iron elements are preferentially migrated to the cathode by electromigration,forming iron phase sinking at the cathode.The study of impurity iron migration behavior in silicon melt under pulsed electric current provides a new approach for the purification of polycrystalline silicon.展开更多
Both density gradient centrifugation and gel electrophoresis have been reported to allow high throughput separation of metallic from semiconducting single-walled carbon nanotubes(SWNTs)when using aqueous sodium dodecy...Both density gradient centrifugation and gel electrophoresis have been reported to allow high throughput separation of metallic from semiconducting single-walled carbon nanotubes(SWNTs)when using aqueous sodium dodecyl sulphate(SDS)suspensions.We show here that both methods rely on an initial dispersion-by-sonication step,which is already selective with respect to electronic structure type.The corresponding aqueous SDS“starting”suspensions obtained after sonication and purifi cation by simple centrifugation(70,000 g,1 h)contain semiconducting SWNTs primarily in the form of small bundles whereas metallic SWNTs are predominantly suspended as individual tubes.Density gradient centrifugation then separates the bundles from the individual tubes on the basis of differences in their overall buoyant densities.Gel electrophoresis separates the longer bundles from the shorter individual tubes on the basis of their different mobilities.We also demonstrate that such starting suspensions can be fractionated according to electronic structure type by even simpler techniques such as size exclusion chromatography or gel fi ltration,thus opening the way for simple scale-up.展开更多
An equation of state (EOS) applicable for both the uniform and non-uniform fluids was established by using the density-gradient expansion, in which the influence parameter x[ρ(r),T] was obtained by the use of dir...An equation of state (EOS) applicable for both the uniform and non-uniform fluids was established by using the density-gradient expansion, in which the influence parameter x[ρ(r),T] was obtained by the use of direct correlation function. The density functional theory (DFT) provides a framework under which both the phase equilibria and interfacial properties can be investigated within a single set of molecular parameters. The phase equilibria inside the critical region can be improved by the renormalization group theory (RGT). However, the correction of interfacial properties by DFT and RGT is computationally difficult. In the present work, the density gradient theory (DGT) in which κ[ρ(r),T] is treated as a constant is used to combine with the RGT for interfacial properties inside the critical region.展开更多
基金supported by the National Natural Science Foundation of China(51971093,52192603,and 51501043)。
文摘The rapid cooling of a metallic liquid(ML)results in short-range order(SRO)among the atomic arrangements and a disordered structure in the resulting metallic glass(MG).These phenomena cause various possible features in the microscopic structure of the MG,presenting a puzzle about the nature of the MGs’microscopic structure beyond SRO.In this study,the nanoscale density gradient(NDG)originating from a sequential arrangement of clusters with different atomic packing densities(APDs),representing the medium-range structural heterogeneity in Zr_(60)Cu_(30)Al_(10)MG,was characterized using electron tomography(ET)combined with image simulations based on structure modeling.The coarse polyhedrons with distinct facets identified in the three-dimensional images coincide with icosahedron-like clusters and represent the spatial positions of clusters with high APDs.Rearrangements of the different clusters according to descending APD order in the glass-forming process are responsible for the NDG that stabilizes both the supercooled ML and the amorphous states and acts as a hidden rule in the transition from ML to MG.
文摘Aim: To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate. Methods: This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up method. These specimens were then cryopreserved. Thirteen specimens were processed by both PureSperm (40 % and 80 %) and Isolate (50 % and 90 %) double density gradient techniques. The two fractions processed by both PureSperm and swim-up were analyzed for post-wash sperm characteristics. Post-thaw analysis was done after 24 hours. Sperm fractions obtained after processing with PureSperm and ISolate were compared for post-wash sperm characteristics and ROS levels. Results: Specimens prepared with PureSperm had significantly higher median total motile sperm counts (TMSC) (32.2 x 10~6 vs. 17.6 x 10~6), recovery rates (69.2 % vs. 50.0 %), and longevity at 4 hours (83.0 % vs. 55.0 %) compared to specimen prepared by swim-up. Post-thaw specimens also had a higher recovery and longevity at 4 hours with PureSperm as compared to the swim-up. Semen specimens processed by PureSperm had significantly higher total sperm count, TMSC, and percentage recovery rates (30.0 % vs. 19.7 %) than ISolate. Conclusion: Semen quality is better preserved in fresh and cryopreserved semen prepared with PureSperm density gradient compared to swim-up. A significant enrichment of sperm is observed with PureSperm compared to ISolate. Higher recovery rates of mature motile sperm obtained after PureSperm sperm preparation may be beneficial for successful ART. i
基金supported by the Special Earthquake Research Project of China Earthquake Administration(201208009)and the National Natural Science Foundation of China(41274083)
文摘We modeled the effect of the deformation of a Density Gradient Zone (DGZ) on a local gravity field using a cubical model and introduced a new method to simulate a complex DGZ (CDGZ). Then, we analyzed the features of the model for the influence of the deformation of the DGZ on the local gravity field. We concluded that land-based gravity is not sensitive to the thickness of the DGZ and that the magnitude of the contribution of the DGZ is one order less than that of the volume strain with the same displacement.
基金supported by the National Natural Science Foundation of China (Grant No. 10734080)the National Basic Research Program of China (Grant No. 2006CB806000)+1 种基金the Chinese Academy of Sciences,the Shanghai Commission of Science and Technology (Grant Nos. 06DZ22015 and 0652nm005)the Hunan Provincial Natural Science Foundation of China (GrantNo. 09JJ3012)
文摘A general solution of the electrostatic potential that determines the maximum light-ion energy is derived for the test-particle acceleration model by taking into account the influence of the substrate-ion density gradient. It is shown that the substrate-ion density structure is also dependent on laser pulse duration. In the picosecond or sub-picosecond regime, the decreasing density gradient of the substrate-ions leads to an evident reduction in the acceleration efficiency of the light-ions. However, this kind of influence is negligible in the ultrashort regime.
基金Grant sponsor:the Science and Technology Projects of Quanzhou,grant number:2019N085Sgrant sponsor:Startup Fund for Scientific Research,Fujian Medical University,grant number:2018QH1100
文摘Objective:Sperm preparation techniques and cryopreservation are widely used in assisted reproductive techniques(ART).How to improve the quality of sperm management is a matter of great concern.Phospholipase C-zeta(PLCζ)is considered a sperm-specific agent that activates oocyte activation and thus playing a crucial role in male fertility.However,the potential mechanisms by which semen processing and cryopreservation on PLCζcontribute to keyhole have not been addressed.Methods:In this study,semen samples were taken from have not been addressed 10 normozoospermic men.Each semen sample was assigned to the following groups:density gradient centrifugation(DGC)as control,microfluidic sorting,and cryopreservation.Sperm parameters of molity,viability,membrane integrity,and intracellular ROS were evaluated during sperm preparation and cryopreservation.The expression of PLCζin human sperm was determined by immunofluorescence and western blotting.Results:The results showed that molity,viability,and membrane integrity decreased in cryopreservation group.Intracellular ROS were also significantly increased compared to the the control group.There was no significant difference between DGC and microfluidic sorting group.Our investigation revealed that total levels of PLCζwere comparable between DGC and microfluidic sorting,but there were significantly reduced levels of PLCζafter cryopreservation as quantified by both immunofluorescenceand immunoblotting.PLCζimmunofluorescence in sperm revealed different PLCζlocalization patterns around the acrosomal(Ac),equatorial(Eq),post-acrosomal(PA)areas of sperm heads,and their combination.The predominant patterns of PLCζlocalization in DGC were similar to that of microfluidic sorting,with strong,with staining.In contrast,PLCζstaining in freeze-thawed sperm was considerably weaker fluorescence intensity.Conclusion:This study clarified the mechanism of sperm preparation and cryopreservation underlying effect on sperm characteristic,accompanied with PLCζexpresion.We demonstrated that microfluidic sorting provides a highly efficient preparation method for clinical selection of PLCζ-expressing sperm comparable to DGC gene expression.It is suggested that the cryopreservation of sperm has a significant detrimental effect on PLCζ.
基金supported by the National Natural Science Foundation of China(Grant No.11772269)。
文摘Based on the dynamic shock response of the material and structure,the hypervelocity impact processes and mechanisms of long composite rods with axial density/impedance gradients penetration into fourlayer targets were studied through experiments and numerical simulation methods.The propagation law of the shock waves,together with the structural responses of the projectiles and targets,the formation and evolution of the fragment groups formed during the processes and their distributions were described.The damage of each target plate was quantitatively analysed by comparing the results of the experiment and numerical simulation.The results showed that the axial density/impedance gradient projectiles could decrease the impact pressure to a certain extent,and the degree of damage to the target plate decreased layer by layer when the head density/impedance of the projectile was high.When the head density/impedance of the projectile was low,the degree of target damage first increased layer by layer until the projectile was completely eroded and then it decreased.The results can provide a reference for the design and application of long rods with axial composite structure for velocities ranging from 6 to 10 Ma or greater.
基金Ministry of Science and Technology of China,National Key R&D Program of China(No.2017YFC1002003 and No.2017YFC1001601)
文摘Objective:The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocytes by intra-cytoplasmic sperm injection(ICSI)following density gradient centrifugation(DGC)and swim-up(SU)procedures.Methods:Semen samples were collected from 30 outpatients who visited the Center for Reproductive Medicine for semen analyses.Following sperm selection by DGC and SU procedures,the liquified semen samples were divided into three groups and incubated at 4,25,and 37°C,respectively.Following incubation for 24,48,and 72 hours,the sperm motility and sperm DNA fragmentation index(DFI)were analyzed.Results:Following the combination of DGC and SU procedures,the sperm motility(91.8%±8.6%vs.50.8%±13.1%)and DFI(5.1%±7.9%vs.13.0%±11.6%)were significantly improved(P<0.01)compared to those without any treatment.The sperm motility of the 3 groups significantly declined(P<0.05)post-incubation compared to that of the groups prior incubation.However,sperm motility significantly increased(76.9%±10.4%)(P<0.05)at 25°C compared to that of the other 2 groups(53.5%±11.0%and 47.6%±10.2%).Sperm DFI significantly increased(P<0.05)at 37°C following incubation for 24 and 72 hours in comparison to that of the other 2 groups.However,the sperm DFI did not significantly increase when the sperm samples were incubated at 4(5.7%±5.9%)and 25°C(6.8%±5.6%)for 24 hours compared to that before incubation(5.1%±7.9%).Conclusions:These results indicate that the sperm quality,in terms of motility and DFI,can be efficiently improved by DGC in combination with SU.Following which,the sperm samples can be incubated at 25°C and be used on the second day for insemination of in vitro matured oocytes by ICSI.
基金the Major State Basic Research Development Program of China 973 Program(2013CB733301)the National Natural Science Fund(41274025) for supporting the work
文摘A three-dimensional density model of the crust and uppermost mantle is determined by the inversion of a set of GOCE gravity and gradients residual anomalies beneath the eastern margin of the Tibetan Plateau and its surrounding areas. In our work, we choose five independent gravity gradients (Txx, Tzz, Txy, Txz, Tyz) to perform density inversion. Objective function is given based on Tikhonov regularization theory. Seismic S-wave velocities play the role of initial constraint for the inversion based on a relationship between density and S-wave velocity. Damped Least Square method is used during the inversion. The final density results offer some insights into understanding the underlying geodynamic processes: (1) Low densities in the margin of the Tibet, along with low wave velocity and resistivity results, yield conversions from soft and weak Tibet to the hard and rigid cratons. (2)The lowest densities are found in the boundary of the plateau, instead of the whole Tibet indicates that the effects of extrusion stress environment in the margin affect the changes of the substance there. The substances and environments conditioning for the earthquake preparations and strong deformation in this transitional zone. (3) Evident low-D anomaly in the upper and middle crust in the Lasha terrane and Songpan-Ganzi terrane illustrated the eastward sub-ducted of southeastern Tibet, which could be accounts for the frequent volcano and earthouakes there.
基金supported by National Natural Science Foundation of China(No.11405271)
文摘The effect of the radial density configuration in terms of width, edge gradient and volume gradient on the wave field and energy flow in an axially uniform helicon plasma is studied in detail. A three-parameter function is employed to describe the density, covering uniform,parabolic, linear and Gaussian profiles. It finds that the fraction of power deposition near the plasma edge increases with density width and edge gradient, and decays in exponential and "bumpon-tail" profiles, respectively, away from the surface. The existence of a positive second-order derivative in the volume density configuration promotes the power deposition near the plasma core, which to our best knowledge has not been pointed out before. The transverse structures of wave field and current density remain almost the same during the variation of density width and gradient, confirming the robustness of the m=1 mode observed previously. However, the structure of the electric wave field changes significantly from a uniform density configuration, for which the coupling between the Trivelpiece-Gould(TG) mode and the helicon mode is very strong, to non-uniform ones. The energy flow in the cross section of helicon plasma is presented for the first time, and behaves sensitive to the density width and edge gradient but insensitive to the volume gradient. Interestingly, the radial distribution of power deposition resembles the radial profile of the axial component of current density, suggesting the control of the power deposition profile in the experiment by particularly designing the antenna geometry to excite a required axial current distribution.
基金the financial support provided by the US Army Research Office under grant number W911NF-18-1-0023.
文摘Density-graded cellular materials have tremendous potential in structural applications where impact resistance is required.Cellular materials subjected to high impact loading result in a compaction type deformation,usually modeled using continuum-based shock theory.The resulting governing differential equation of the shock model is nonlinear,and the density gradient further complicates the problem.Earlier studies have employed numerical methods to obtain the solution.In this study,an analytical closed-form solution is proposed to predict the response of density-graded cellular materials subjected to a rigid body impact.Solutions for the velocity of the impinging rigid body mass,energy absorption capacity of the cellular material,and the incident stress are obtained for a single shock propagation.The results obtained are in excellent agreement with the existing numerical solutions found in the literature.The proposed analytical solution can be potentially used for parametric studies and for effectively designing graded structures to mitigate impact.
基金support of the Deutsche Forschungsgemeinschaft(DFG),Grant no.AL 1343/7–1,AL 1343/8–1,Yi 103/3–1。
文摘Critical properties of metallic materials,such as the yield stress,corrosion resistance and ductility depend on the microstructure and its grain size and size distribution.Solute atoms that favorably segregate to grain boundaries produce a pinning atmosphere that exerts a drag pressure on the boundary motion,which strongly affects the grain growth behavior during annealing.In the current work,the characteristics of grain growth in an annealed Mg-1 wt.%Mn-1 wt.%Nd magnesium alloy were investigated by advanced experimental and modeling techniques.Systematic quasi in-situ orientation mappings with a scanning electron microscope were performed to track the evolution of local and global microstructural characteristics as a function of annealing time.Solute segregation at targeted grain boundaries was measured using three-dimensional atom probe tomography.Level-set computer simulations were carried with different setups of driving forces to explore their contribution to the microstructure development with and without solute drag.The results showed that the favorable growth advantage for some grains leading to a transient stage of abnormal grain growth is controlled by several drivers with varying importance at different stages of annealing.For longer annealing times,residual dislocation density gradients between large and smaller grains are no longer important,which leads to microstructure stability due to predominant solute drag.Local fluctuations in residual dislocation energy and solute concentration near grain boundaries cause different boundary segments to migrate at different rates,which affects the average growth rate of large grains and their evolved shape.
文摘Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A total of 153 infertile men were involved. Conventional semen parameters and sperm chromatin structure assay (SCSA) parameters, that is, DNA fragmentation index (%DFI) and high DNA stainability (%HDS), were assessed on the flesh ejaculated semen samples, which were treated and incubated under different conditions. Negative correlations were identified between the %DFI and sperm concentration, motility, progressive motility and morphology. A lower percentage of DFI was detected in spermatozoa when density gradient centrifugation (DGC) was followed by swimup treatment in comparison with DGC alone (P 〈 0.01). Although the %DFI increased in a time-dependent manner with incubation both at room temperature (RT) and at 37℃ in air, the %DFI after 24 h at RT was significantly lower than that at 37℃ (P 〈 0.05). Incubation with 5% CO2 was effective in maintaining sperm motility (P 〈 0.01); however, it induced further elevation of %DFI (P 〈 0.001). Thus, sperm DNA damage was associated with longer incubation periods. Interestingly, common culture conditions, such as maintaining pH and temperature, compromised the sperm DNA integrity.
文摘The hybrid finite analytic(HFA) method is a kind of numerical scheme in rectangular element. In order to simulate the shallow circulation in irregular bathymetry by HFA scheme, the model in sigma coordinate system was obtained. The model has been tested against three cases: 1) Wind induced circulation; 2) Density driven circulation and 3) Seiche oscillation. The results obtained in the present study compare well with those obtained from the corresponding analytical solutions under idealized for the above three cases. The hybrid finite analytic method and the circulation model in sigma coordinate system can be used calculate the flow and water quality in estuaries and coastal waters.
文摘Engineering-oriented simulations of quantum mechanical tunneling are often based on density-gradient (DG) theory. This paper presents an analytical solution to the DG equation for quantum tunneling through an ultra-thin oxide in a MOS capacitor with an n+ poly-silicon gate obtained using the method of matched asymptotic expansions. Tunneling boundary conditions extend the approximation into the entire region of the poty-silicon gate, oxide barrier, and substrate. An analytical solution in the form of an asymptotic series is obtained in each region by treating each part of the domain as a separate singular perturbation problem. The solutions are then combined through 'matching' to obtain an approximate solution for the whole domain. Analytical formulae are given for the electrostatic potential and the electron density profiles. The results capture the features of the quantum effects which are quite different from classical physics pre- dictions. The analytical results compare well with exact numerical solutions over a broad range of voltages and different oxide thicknesses. The analytical results predict the enhancement of the quantum tunneling effect as the oxide thickness is reduced.
基金supported by Shenzhen Clean Energy Research Institutesupported by the National Key R&D Program of China (No. 2017YFE0301205)National Natural Science Foundation of China (Nos. 11875289, 11975271, 11605244, 11675211, 12075284, 12075283, 12075155 and 11875294)
文摘The influence of a molybdenum dust buildup on plasma edge turbulence has been studied in the EAST tokamak.The motion of the dust from the upper divertor region is detected by a fast visible CCD camera,the XUV spectrometer arrays,and the EUV spectrometer.The MoXV emission intensity sharply increases compared with the spectral lines of various ionization states of other elements,which implies that the dust particles are the molybdenum impurities.The radial distribution of Mo^(14+)ion simulated by a simplified 1 D transport model indicates that the molybdenum dust mainly deposits in the pedestal bottom region.Moreover,it is observed that the coherent mode(CM)appears atρ=0.94 after the molybdenum impurities enter the main plasma region.The influx of molybdenum impurities results in increasing pedestal electron density and decreasing pedestal electron temperature in contrast to that before the event of impurities dropping.It is also found that the electron density gradient in the pedestal increases when the ablation of the molybdenum impurities is observed in the pedestal region.The qualitative experimental results indicate that the onset of CM is likely related to the increase of the density gradient and edge collisionality in the pedestal.In comparison to the density gradient,the enhancement of CM amplitude largely depends on the increase of the edge collisionality.
基金supported by a grant from Regione Abruzzo (P.O. FSE Abruzzo 2007 – 2013, Action 4, Type 4.1)the Provincial Breeders Federation of Trento, especially Dr. Claudio Valorz, for their support
文摘Background: Density gradient centrifugation was reported as a technique of semen preparation in assisted reproductive techniques in humans and animals. This technique was found to be efficient in improving semen quality after harmful techniques such as cryopreservation. Recently a modified technique, single layer centrifugation,was proposed as a technique providing a large amount of high quality spermatozoa, and this treatment was performed before conservation. Single layer centrifugation has been studied prevalently in stallions and in boars,but limited data were available for bulls. Occasionally bulls are known to experience a transient reduction in semen quality, thus techniques that allow improvement in semen quality could be applied in this context. The aim of this study was the evaluation of single layer and double layer centrifugation by the use of iodixanol, compared with conventional centrifugation and non-centrifuged semen, on the sperm characteristics during the cryopreservation process in bulls with normal and poor semen quality.Results: Single layer centrifugation and double layer centrifugation both significantly increased the percentage of normal spermatozoa and decreased the percentage of non-sperm cells in poor quality samples, while both were ineffective in those of normal quality. Sperm characteristics in poor quality samples increased after single layer centrifugation and double layer centrifugation, reaching values similar to those recorded in normal samples, and this trend is maintained after equilibration and after cryopreservation. On the other hand, SLC and DLC resulted in a consistent reduction in the spermatozoa recovered, and this resulted in a reduction of the absolute amount of spermatozoa cryopreserved in the normal samples, without a clear improvement in sperm characteristics in this type of sample.Conclusions: These data suggested that both SLC and DLC could be performed in practice, but their application should be limited to the cases in which the quality of the spermatozoa recovered is more important than the total amount of spermatozoa.
文摘Testicular sperm extraction is a common procedure used to find spermatogenic cells in men with nonobstructive azoospermia. The laboratory processing of biopsied testicular tissues needs to be performed meticulously to acquire a high yield of cells. In this study, the effectiveness of mincing the tissues after testicular biopsy was assessed using histological evaluation, as was the possible adverse effect of residual tissue on the migration of spermatogenic cells during density gradient centrifugation. Our results indicate that testicular residual tissue, when laid on the density gradient medium along with the sperm wash, hinders the spermatogenic cells' forming a pellet during centrifugation, and therefore impairs the intracytoplasmic sperm injection procedure. Whereas the mean number of recovered cells from the sperm wash medium (SWM) with residual tissue is 39.435 ~ 24.849, it was notably higher (60.189 ~ 28.214 cells) in the SWM without minced tissues. The remaining tissue contained no functional seminiferous tubules or spermatogenic cells in histological sections. In conclusion, the remaining residual tissue after mincing biopsied testicular tissue does not add any functional or cellular contribution to spermatogenic cell retrieval; in fact, it may block the cellular elements in the accompanying cell suspension from migrating through the gradient layers to form a pellet during centrifugation and cause loss of spermatogenic cells.
基金financially supported by the National Natural Science Foundation of China(No.U21B2082)Natural Science Foundation of Beijing Municipality(No.2222065)and Fundamental Research Funds for the Central Universities(No.FRF-TP-22-02C2).
文摘The impurity iron in silicon material will seriously affect the photoelectric conversion efficiency of silicon solar cells.However,the traditional silicon purification method has the disadvantages of long cycle,high energy consumption and serious pollution.In this study,an efficient and green pulsed electric current purification technology is proposed.The electromigration effect of iron elements,the current density gradient driving of iron phase,and the gravity of iron phase all affect the migration behavior of iron phase in silicon melt under pulsed electric current.Regardless of the depth of electrode insertion into the silicon melt,the solubility of iron in silicon decreases under the pulsed electric current,which helps to form the iron phase.At the same time,the iron phase tends to sink toward the bottom under the influence of gravity.When the electrode is shallowly inserted,a non-uniform electric field is formed in the silicon melt,and the iron phase is mainly driven by the current density gradient to accelerate sink toward the bottom.When the electrode is fully inserted,an approximately uniform electric field is formed in the silicon melt,and iron elements are preferentially migrated to the cathode by electromigration,forming iron phase sinking at the cathode.The study of impurity iron migration behavior in silicon melt under pulsed electric current provides a new approach for the purification of polycrystalline silicon.
基金by the Bundes-ministerium für Bildung und Forschung(BMBF)by the Deutsche Forschungsgemeinschaft(DFG).
文摘Both density gradient centrifugation and gel electrophoresis have been reported to allow high throughput separation of metallic from semiconducting single-walled carbon nanotubes(SWNTs)when using aqueous sodium dodecyl sulphate(SDS)suspensions.We show here that both methods rely on an initial dispersion-by-sonication step,which is already selective with respect to electronic structure type.The corresponding aqueous SDS“starting”suspensions obtained after sonication and purifi cation by simple centrifugation(70,000 g,1 h)contain semiconducting SWNTs primarily in the form of small bundles whereas metallic SWNTs are predominantly suspended as individual tubes.Density gradient centrifugation then separates the bundles from the individual tubes on the basis of differences in their overall buoyant densities.Gel electrophoresis separates the longer bundles from the shorter individual tubes on the basis of their different mobilities.We also demonstrate that such starting suspensions can be fractionated according to electronic structure type by even simpler techniques such as size exclusion chromatography or gel fi ltration,thus opening the way for simple scale-up.
基金Project Supported by the National Naturai Science Foundation of'C-hina (No. 20576030) and the Key Program Foundation from the North China Electric Power University.
文摘An equation of state (EOS) applicable for both the uniform and non-uniform fluids was established by using the density-gradient expansion, in which the influence parameter x[ρ(r),T] was obtained by the use of direct correlation function. The density functional theory (DFT) provides a framework under which both the phase equilibria and interfacial properties can be investigated within a single set of molecular parameters. The phase equilibria inside the critical region can be improved by the renormalization group theory (RGT). However, the correction of interfacial properties by DFT and RGT is computationally difficult. In the present work, the density gradient theory (DGT) in which κ[ρ(r),T] is treated as a constant is used to combine with the RGT for interfacial properties inside the critical region.