Expression of Skin Barrier Protein Filaggrin in Skin Diseases without Atopic Dermatitis

The epidermis represents an essential barrier versus a broad range of exogenous stimuli. To form a functional epidermis, keratinocytes express filaggrin which plays a vital role in atopic dermatitis. However the relationship between filaggrin and other skin diseases remains unknown. In our study we chose 5 different common skin diseases and analyzed the expression of filaggrin in the skin using immunohistochemistry. Imiquimod (IMQ)-induced mouse model was used for detecting the filaggrin level and barrier function. The results indicated that the expression of filaggrin is reduced in psoriasis compared to the other skin diseases. Furthermore in vivo study showed that the skin barrier is defected with a decreased expression of filaggrin in IMQ-induced psoriasis mouse model, which is accompanied with an increased level of IL-17/IL-23. In conclusion, the defective skin barrier is involved in the development of psoriasis in human and mice with a reduced expression of filaggrin which may be regulated by the increased level of IL-17/IL-23 in the skin.

The effect of protective genital care protocol on preventing diaper dermatit development in 0 - 18 month old children using antibiotics

Diaper dermatit is among the widely seen inflamatory skin diseases in neonatals and small 0 - 18 month old children. Control of causative agents as well as providing good genital care may prevent or reduce the risk of the disease. The aim of this study is find out the effect of protective genital care on reducing the diaper dermatit development in 0 - 18 months old inpatient 0 - 18 month old children who are using antibiotic. The study was conducted as prospective half-experimetal using control-experimental groups. Our samples comprise of 0 - 18 month old children admitted at Dokuz Eylul University Practical and Research Hospital, pediatric clinic between January and May 2009, 41 0 - 18 month old children as a control group were not under protective genital care, and 42 0 - 18 month old children as an experimental group were under the protecion and using vaseline. The mothers of the experimental group 0 - 18 month old children were given education. All 0 - 18 month old children were followed-up 3 - 7 days and the collected data were evaluated in computer using Pearson’s chisquare, Fisher’s exact chi-square and Yatest-corrected chi-square tests. The diaper dermatit develop- ment ratio was 34.1% in the control group and 7.1% in the experimental group

湿疹样损害与银屑病样损害的免疫漂移

免疫漂移是指在用生物制剂等药物治疗银屑病(psoriasis,PsO)或特应性皮炎(atopic dermatitis,AD)时,导致Th1/Th17与Th2之间反应不平衡,细胞因子水平的变化可能反而有利于向相反的皮肤疾病转化,PsO患者出现湿疹样皮损,AD患者出现PsO样皮损。依那西普、英夫利昔单抗、阿达木单抗、司库奇尤单抗、依奇珠单抗、乌司奴单抗、古塞奇尤单抗、度普利尤单抗等生物制剂和JAK抑制剂如乌帕替尼均有诱发免疫漂移的报告。对于既往有特应性个人史或家族史的PsO患者,以及有PsO个人史及家族史的AD患者均应警惕此不良反应的发生。皮肤组织病理是诊断的金标准。对于轻度皮损,可外用糖皮质激素治疗;如果局部外用疗效不佳,或皮损严重程度为中度以上,难以控制时则应停用原药物,选择对两种疾病都有效的疗法如光疗、环孢素、甲氨蝶呤、霉酚酸酯。

培土清心颗粒的抗炎效果研究

目的:评价培土清心颗粒对实验性炎症动物模型的抗炎效果。方法:选择SPF级NIH小鼠及SPF级SD大鼠,采用区组随机实验方法进行分组,包括正常对照组、模型对照组、阳性药物对照组以及培土清心颗粒高、中、低剂量组,分别创建二甲苯致小鼠耳廓肿胀、冰醋酸致小鼠腹腔毛细血管通透性增高以及大鼠棉球肉芽肿3种动物模型,对造模成功后的动物进行灌胃,观察评价培土清心颗粒的抗炎作用。结果:培土清心颗粒对二甲苯所致小鼠耳廓肿胀、冰醋酸所致小鼠腹腔毛细血管通透性增高及棉球所致大鼠肉芽组织增生均有明显抑制作用。结论:培土清心颗粒具有良好的抗急慢性炎症效果,其抗炎作用与西药吲哚美辛肠溶片和中成药消风止痒颗粒的效果相当。

培土清心颗粒对豚鼠皮肤瘙痒模型的止痒效果研究

目的:观察评价培土清心颗粒对实验性瘙痒动物模型的止痒效果。方法:采用动物实验研究方法,观察培土清心颗粒对磷酸组胺所致豚鼠瘙痒模型、4-氨基吡啶所致小鼠瘙痒模型和右旋糖酐40诱发小鼠瘙痒模型的影响,观察培土清心颗粒的止痒作用。结果:培土清心颗粒可增加豚鼠涂抹磷酸组胺次数(P<0.01),升高豚鼠致痒阈值(P<0.01),减少4-氨基吡啶致瘙痒小鼠和右旋糖酐40诱发瘙痒小鼠的搔抓次数(P<0.01,P<0.05)。结论:培土清心颗粒可通过减少内源性和外源性组胺释放途径缓解皮肤瘙痒症状,对特应性皮炎具有良好的止痒效果。

奥美拉唑联合克拉霉素、阿莫西林治疗口周皮炎疗效观察

目的探讨奥美拉唑联合克拉霉素,阿莫西林治疗口周皮炎的疗效。方法 90例口周皮炎患者随机分为两组,两组均外涂氯硫霜,3次/d。同时,治疗组口服奥美拉唑及克拉霉素,阿莫西林,两组疗程均为10天,疗程结束后随访6个月,判定疗效并观察复发率。结果治疗组有效率为89.13%,对照组为63.64%;治疗组复发率为11.11%,对照组为83.33%。两组有效率及复发率差异均有统计学意义(P均<0.005)。结论奥美拉唑联合克拉霉素、阿莫西林治疗口周皮炎疗疗效显著,复发率低。

药物性皮炎100例临床资料分析

本文分析由1970～1988年收集的100例药物性皮炎。致病药物以抗生素居首位,其次为镇痛类药物和磺胺类药物,中草药致病不容忽视。其发病机理考虑为变态反应。全身反应有发热、肝炎样反应、肾炎样反应、心血管和血液学反应。皮肤反应多种多样,以固定性红斑、发疹型、荨麻疹型和剥脱性皮炎型多见。本组均采用皮质类固醇激素治疗。97%于1周至2月内痊愈,3%死于败血症。本文就病因、发病机理、临床特点、诊断及治疗等进行有关文献复习和讨论。

培土清心颗粒对特应性皮炎样动物模型的影响

目的:建立特应性皮炎样小鼠模型,探讨中药培土清心颗粒对特应性皮炎细胞免疫的作用机制。方法:以半抗原2-氟二硝基甲苯(DNFB)诱导C57BL/6小鼠建模,造模第1天开始灌胃给药,连续14d。根据局部皮肤症状与组织病理学进行模型评估,计算耳廓肿胀程度和流式细胞检测脾脏调节性B细胞表达变化。结果:模型组皮肤出现明显炎性改变,耳部肿胀明显,病理提示表皮增厚、真皮炎性细胞浸润。培土清心颗粒炎性反应抑制率为82.73%;培土清心颗粒组脾脏CD5+B细胞、CD1d+CD5+CD19+B细胞阳性表达率均高于模型组,组间比较差异具有统计学意义(P<0.01)。结论:中药培土清心颗粒能有效治疗特应性皮炎,其治疗作用与抗炎、刺激调节性B细胞增殖有关。