Effect of germicidal UV-C light(254 nm)on eggs and adult of house dustmites,Dermatophagoides pteronyssinus and Dermatophagoides farinae(Astigmata:Pyroglyhidae)

Objective:To examined the immediate and 24 hours post-irradiation germicidal effects of UV-C lamp on.eggs and adults of house dust mites Dermatophagoides pteronyssinus(D.pteronyssinus)and Dermatophagoides farinae(D.farinae).Methods:This study investigated the immediate and24 hours post irradiation mortalities of adult mites exposed to UV-C at different exposure times(5mins,10 mins,15 mins,20 mins,30 mins and 60 mins)and distances(10 cm,25 cm,33 cm,45 cm and 55 cm).Fresh eggs of the 2 dust mites were also irradiated at 10,35 and 55 cm for 0.5,1,2,3,and 5 minutes,and observed daily post-irradiation for up to 7 days.Results:Highest immediate mortality of 100%occurred with direct irradiation al 10 cm distance from UV-C lamp and for 60mins,for both species of mites.The post 24 hours mean mortality rates were(58.4±17.4)%for D.pteronyssinus and(27.7±9.7)%for D.fitrinae when irradiated for 1 hour at 55 cm distance under UV-C lamp.When mites were irradiated in the presence of culture media,the highest mortality rates were lower compared to the direct irradiation;at 10 cm distance and 60 mins exposure,the mean mortality was(74.0±6.8)%for D.pteronyssinus and(70.3±6.7)%for D.farinae.Egg hatchability for both species of mites was also notably reduced by greater than 50%following irradiation.Conclusions:Ultraviolet C irradiation is lethal to an array of organisms by damaging their nucleic acids(DNA and RNA).This study demonstrates the increasing mite mortalities with increasing exposure times and decreasing distances.

Effect of a commercial air ionizer on dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae(Acari:Pyroglyphidae) in the laboratory

Objective:To investigate the short and long tenn efficacy of a commercial air ionizer in killing Dermatophagoides pteronyssinus(D.pteronyssinus)and Dermalophagoides farinae(D.farinae)mites.Methods:The effect of a commercial ionizer on D.pteronyssinus and D.farinae was evaluated in the laboratory,using a specially designed test.Mortality was assessed after 6,16and 24 hours for direct exposure and after 24,36,48,60 and 72 hours for exposure in simulated mattress.New batches of mites were used for each exposure time.Results:LT_(50)for direct exposure of ionizer was 10 hours for D.pteronyssinus and 18 hours for D.farinae.The LT_(50)for exposure in simulated mattress was 132 hours or 5.5 days for D.pteronyssinus and 72 hours or 3days for D.farinae.LT_(95)for direct exposure of ionizer was 36 hours for D.pteronyssinus and D.farinae.Meanwhile,the LT_(95)for exposure in simulated mattress was 956 hours or 39.8 days for D.pteronyssinus and 403 hours or 16.8 days for D.farinae.Conclusions:This study demonstrates the increasing mite mortalities with increasing exposure time of a commercial ionizer and suggests that negative ions produced by an ionizer kill dust mites and can be used to reduce natural mile populations on exposed surfaces such as floors,clothes,curtains,etc.However,there is reduced efficacy on mites inside stuffed materials as in mattresses and furniture.

Effects of dermatophagoides farinae drops combined with pidotimod on inflammatory response and immune response in children with allergic rhinitis combined with asthma

Objective: To investigate the effects of dermatophagoides farinae drops combined with pidotimod on inflammatory response and immune response in children with allergic rhinitis combined with asthma. Methods: A total of 118 children with allergic rhinitis combined with asthma who were treated in our hospital between December 2014 and May 2017 were selected as the research subjects and divided into the control group (n=59) and the dermatophagoides farinae drops group (n=59) by random number table method. Control group received both conventional therapy and pidotimod therapy, and dermatophagoides farinae drops group received both conventional therapy and dermatophagoides farinae drops combined with pidotimod therapy. The differences in inflammatory factor, cellular immunity index and humoral immunity index levels were compared between the two groups before treatment (T0), after 3 months of treatment (T1), after 6 months of treatment (T2) and after 12 months of treatment (T3). Results: At T0, inflammatory factor, cellular immunity index and humoral immunity index levels were not significantly different between the two groups. At T1, T2 and T3, serum inflammatory factor IL-2 contents of dermatophagoides farinae drops group were higher than those of control group at the corresponding time points whereas IL-4, IL-5, IL-9 and IL-18 contents were lower than those of control group at the corresponding time points;peripheral blood cellular immunity indexes CD3+, CD4+, CD8+ and CD4+/CD8+levels were higher than those of control group at the corresponding time points;serum humoral immunity indexes IgA, IgM and IgG contents were higher than those of control group at the corresponding time points whereas IgE contents were lower than those of control group at the corresponding time points. Conclusion: Dermatophagoides farinae drops combined with pidotimod therapy can effectively reduce the inflammatory response degree and optimize the cellular and humoral immune function of the children with allergic rhinitis combined with asthma.

Assessment of the ventilation function and serum biochemical indexes after sublingual dermatophagoides farinae drops combined with loratadine treatment of children with asthma and allergic rhinitis

Objective:To study the ventilation function after sublingual dermatophagoides farinae drops combined with loratadine treatment of children with asthma and allergic rhinitis and the influence on serum biochemical indexes.Methods: A total of 40 children with asthma and allergic rhinitis treated in our hospital between September 2013 and March 2015 were collected and divided into the control group (n=22) who accepted loratadine therapy alone and the observation group (n=18) who accepted sublingual dermatophagoides farinae drops combined with loratadine therapy after the treatment was reviewed. Before treatment and after 6 months and 1 year of treatment, spirometer was used to test ventilation function indexes;ELISA method was used to determine the contents of inflammatory mediators;RIA method was used to determine the contents of airway remodeling indicators.Results:Before treatment, differences in ventilation function index levels as well as inflammatory mediator and airway remodeling index contents were not statistically significant between two groups of children. After 6 months and 1 year of treatment, FEV1, FVC and PEF levels of observation group were higher than those of control group;serum IL-2 content was higher than that of control group while IL-5, IL-17 and IL-33 contents were lower than those of control group;serum PDGF-BB, TGF-β1 and NF-κB contents were lower than those of control group.Conclusion:Sublingual dermatophagoides farinae drops combined with loratadine therapy can optimize the ventilation function, reduce the systemic inflammatory response and inhibit airway remodeling in children with asthma and allergic rhinitis.

粉尘螨Ⅰ类变应原(Der fⅠ)的克隆表达、纯化及免疫学特性

粉尘螨Ⅰ类变应原(Der fⅠ)是粉尘螨Dermatophagoidesfarinae主要变应原,可引发Ⅰ型变态反应。挑取纯培养的粉尘螨,提取总RNA,采用RT-PCR方法有效地扩增出DerfⅠ片段,产物连接入T载体(pMD18)。经扩增后,用NdeⅠ和XhoⅠ双酶切后将目的片段连接到pET24a表达载体上,得到重组质粒pET24a-DerfⅠ,工程菌经IPTG诱导培养,高效表达出DerfⅠ蛋白,该蛋白主要以包涵体形式存在。表达产物经亲和层析纯化,用Westernblot及ELISA方法检测免疫学活性,结果表明目的蛋白具有良好的免疫原性。

粉尘螨Ⅰ类变应原(Der fⅠ)的cDNA克隆及序列分析

目的获得我国广州地区粉尘螨 类变应原 (Der f ) c DNA片段 ,为构建 DNA疫苗或表达重组蛋白打下基础。方法挑取经选择鉴定的活粉尘螨 ,提取总 RNA,采用 RT- PCR的方法扩增 Der f 片断 ,进行克隆、测序和分析。结果获得长度为 6 32个碱基对的核苷酸片断 ,序列分析结果和 Genebank上去除内含子后的基因序列 (em b| X6 5 196 .1| )同源性为99% ,其中有 6个碱基不同 ,推导的编码氨基酸序列同源性为 10 0 %。结论我们首次获得广州地区的 Der f 的 c DNA克隆 ,该克隆 c DNA序列与 Genebank上已公布的 Der f 序列高度同源。

粉尘螨Ⅰ类变应原的cDNA克隆测序及亚克隆

目的 获得粉尘螨I类变应原 (Derf 1)cDNA克隆及亚克隆 ,并进行测序。 方法 设计合成引物 ,从粉尘螨体内提取RNA ,经逆转录聚合酶链反应 (RT PCR)获得cDNA ,PCR扩增目的片段经纯化回收后克隆至pMD 18T ,转化大肠埃希菌 (E .coli)JM10 9,经PCR初筛挑选阳性克隆并测序 ,将PCR筛检阳性重组子及pET 3 2a( +)表达载体分别用BamHⅠ和SacⅠ双酶切 ,连接转化至E .coli感受态细胞JM10 9中过夜培养 ,挑选菌落进行酶切鉴定。 结果 从粉尘螨基因组RNA中扩增出Derf 1基因 ,获得pET 3 2a( +) Derf 1亚克隆 ,酶切产物的大小与预期相符。 结论 对粉尘螨Derf 1基因进行体外扩增并获得pET 3 2a( +) Derf 1亚克隆。

粉尘螨滴剂对Ⅰ型变态反应的影响

目的考察粉尘螨滴剂时Ⅰ型变态反应的影响。方法2，5，10倍临床拟使用剂量的粉尘螨滴剂于大鼠或豚鼠舌下给药4～5周，进行大鼠的同种被动皮肤过敏反应（PCA反应）实验、豚鼠支气管哮喘实验、致敏豚鼠离体回肠平滑肌过敏性收缩实验（Sehultz Dale test）、大鼠肥大细胞释放组胺实验和致敏大鼠肺中白细胞浸润实验。结果粉尘螨滴剂按拟定的临床给药方案给药4-5周能够明显减小大鼠PCA反应的蓝斑直径和染料的渗出，明显延长卵白蛋白致豚鼠死亡时间，降低豚鼠死亡率，抑制豚鼠回肠Schultz Dale反应时的收缩（P〈0．05），抑制抗原再次刺激时大鼠肥大细胞释放组胺，明显减少致敏大鼠抗原再次刺激时肺内过敏性白细胞渗出（P〈0．01）。结论粉尘螨滴剂舌下给药4—5周能抑制Ⅰ型变态反应。

粉尘螨Ⅰ类抗原cDNA的克隆表达和初步鉴定

目的 构建粉尘螨Ⅰ类抗原 (Derf1)cDNA基因的重组表达质粒 ,并于E .coli表达。方法 用BamHⅠ和SacⅠ从重组质粒pMD 18T Derf 1上切下Derf1基因 ,插入表达载体pET32a(+)质粒 ,转化大肠杆菌BL2 1,在氨苄青霉素阳性的LB平板上筛选阳性重组子 ,并经双酶切及PCR扩增鉴定。重组质粒pET32a(+) Derf 1转化大肠杆菌 ,IPTG诱导表达后进行SDS PAGE电泳和薄层凝胶扫描定量分析。结果 对重组质粒进行酶切和PCR鉴定 ,与预期结果相符 ,证明已成功构建携带Derf1基因的重组原核表达质粒pET32a(+) Derf 1。核酸序列测定及同源性分析证实所构建的原核表达质粒pET32a(+) Derf1中所含的Derf1基因与GenBank中的Derf1序列同源性达到 99.5 %。Derf 1基因在大肠杆菌诱导表达后获得Mr 约4 5 0 0 0的蛋白 ,蛋白含量占全菌体蛋白含量的 15 %。结论 成功构建了粉尘螨Ⅰ类抗原cDNA基因的重组表达质粒pET32a(+) Derf 1,并在大肠杆菌中获得高效表达 ,为获得重组纯化Derf

粉尘螨Ⅰ、Ⅱ类抗原(Derf1,Derf2)的cDNA克隆测序

粉尘螨Derf1、Derf2cDNA的克隆及测序分析。设计合成引物,常规抽提粉尘螨螨体总RNA,RT-PCR获得cDNA,经纯化回收后分别克隆至pMD-18T,转化大肠杆菌E.coliJM109,经PCR初筛挑选阳性克隆并测序。从粉尘螨基因组RNA中扩增出Derf1、Derf2cDNA片段,分别测得其核苷酸序列。Derf1cDNA测序结果与GenBank提供的Derf1基因(emb|X65196.1)去掉内含子后的序列经Blast分析,发现它们核苷酸同源性为99.52%,而Derf2cDNA序列与郝敏麒等报道的广州地区粉尘螨Derf2的cDNA序列(AF346905)相比,未发现有插入序列。成功地对粉尘螨Derf1、Derf2cDNA进行克隆测序,发现不同动物区系粉尘螨的Derf1、Derf2cDNA序列存在差异。

粉尘螨Ⅰ类变应原瞬时表达载体的构建及其在烟草中的表达

目的构建粉尘螨I类变应原瞬时表达载体TRBO-Der f1并观察其在烟草中的表达。方法以粉尘螨总RNA为模板,采用RT-PCR方法扩增Der f1基因并定向克隆到瞬时表达载体TRBO中,转化根癌农杆菌(Agrobacterium tumefa-ciens)GV1301株后,抽提质粒进行酶切及PCR鉴定。将含TRBO-Der f1重组质粒的GV1301注射本氏烟叶片,SDS-PAGE电泳检测注射3、4、5、6d后Der f 1的表达,并进行Western blot鉴定。结果电泳及测序表明Der f1基因克隆成功,大小为627bp。经PCR及酶切结果证实重组质粒TRBO-Der f1成功转入根癌农杆菌。SDS-PAGE电泳检测表明,该蛋白于第5和6d在烟草叶片中高表达,并通过Western blot得到验证。结论粉尘螨I类变应原Der f 1成功表达于烟草叶片,为进一步研究源于植物的粉尘螨疫苗奠定了基础。

粉尘螨Ⅰ类变应原成熟肽段基因的克隆和生物信息学分析

目的了解不同地区粉尘螨Ⅰ类变应原(Der fⅠ)成熟肽段基因序列的变异性及其编码蛋白的化学及免疫学特征。方法从皖南地区分离鉴定的活粉尘螨,提取总RNA,采用RT-PCR扩增Der fⅠ成熟肽段基因,克隆到T载体测序确认。应用生物信息学软件对该基因进行初步分析。结果获得皖南地区两种不同基因型的Der fⅠ成熟肽段基因,与GenBank公布的其他地区Der fⅠ基因序列比较同源性在98.73%～99.84%之间;相应编码氨基酸序列的同源性在99.04%～100%之间,存在6个氨基酸残基变异位点,并影响其抗原表位的改变。结论不同地区Der fⅠ成熟肽段基因序列及其编码氨基酸序列存在差异,并对其免疫原性产生影响。克隆并分析研究我国各地的尘螨变应原基因序列及氨基酸序列变化,对于临床变应性疾病的诊治有重要的意义。

Cloning, sequence analysis and expression in E. coli of the group 3 allergen of Dermatophagoides farinae

Background The dust mites, which are mostly represented by Dermatophagoides spp. （Acari： Pyroglyphidae）, are the major sources of indoor allergens. Identification and characterization of these mite allergen molecules are an important step in the development of new effective diagnostic procedures and possible therapeutic strategies for allergic disorders associated with dust mites. Methods Total RNA was extracted from Dermatophagoides farinae. The gene coding for Der f 3 was amplified by RT-PCR with the primers designed based on previous sequence published in GenBank. The target gene was cloned intermediately into pMD19-T plasmid and finally into plasmid pET28a （＋）, expressed in E. coil BL21 at the aid of the inducer isopropyI-D-thiogalactopyranoside （IPTG）. The physicochemical properties, spatial structure of the allergen were analyzed with bioinformatics software. Results The cDNA coding for group 3 allergen of Dermatophagoides farinae from China was cloned and expressed successfully. Sequencing analysis showed that there were nineteen mismatched nucleotides in five Der f3 cDNA clones in comparison with the reference （GenBank Accession No. AY283291）, which resulted in deduced amino acid sequence incompatibility in eleven residues. Bioinformatics analysis revealed that the Der f 3 pro-protein was an extracellular hydrophobic protein, consisting of 259 amino acids with a 16 amino acid signal peptide. The protein was deduced to have three chymotrypsin active sites （53-68 AA, 108-122 AA and 205-217 AA）, one N-glycosylation site, one cAMP- and cGMP-dependent protein kinase phosphorylation site, four protein kinase C phosphorylation sites, two casein kinase II phosphorylation sites, and five N-myristoylation sites. Conclusions Der f3 is an extracellular hydrophobic protein which possesses multiple activation and phosphorylation sites. Polymorphism may exist in the Der f3 gene but this needs to be further confirmed in the future.

Allergenicity assay of allergen from Dermatophagoides farinae in transgenic tobacco

Derf2 gene for one of mite allergens in Dermatophagoides farinae has been cloned and expressed under regulation of 35S promoter in transgenic tobacco. The tran-scriptional analysis showed that this mite complete gene structure in genomic sequence could be spliced at prediction site. Allergenicity assay with immunological sera indicated that the extracts from the transgenic tobacco gave obvious positive IgE binding reaction with specific serum pool. This work would be of potential use in allergenicity assessment of genetically modified food.

粉尘螨滴剂治疗单一与多重致敏的变应性鼻炎的临床疗效及对嗜酸粒细胞影响的研究

目的探讨粉尘螨滴剂治疗单一与多重致敏的变应性鼻炎(AR)的临床疗效及对嗜酸粒细胞的影响。方法回顾性分析2020年9月~2022年9月江苏省如皋市人民医院收治的142例AR患者的临床资料,均实施粉尘螨滴剂治疗,依据患者皮肤点刺试验变应原数量将单一致敏者68例纳入单一组,将多重致敏者74例纳入多重组。比较治疗前后鼻部症状及药物评分、治疗1年后疗效、鼻灌洗液中嗜酸粒细胞阳性率及不良反应。结果两组治疗6个月、治疗12个月后鼻塞、鼻痒、流涕、喷嚏、鼻部症状总分、药物总评分均明显降低(P<0.05),两组间比较差异无统计学意义(P>0.05);两组治疗1年后总有效率、鼻灌洗液中嗜酸粒细胞阳性率比较,差异无统计学意义(P>0.05);两组不良反应总发生率比较,差异无统计学意义(P>0.05)。结论粉尘螨滴剂治疗单一与多重致敏的AR具有相似疗效,可明显减轻患者临床症状,安全可靠。

不同年龄段患者经舌下含服粉尘螨滴剂治疗变应性鼻炎的疗效评估

目的评价不同年龄段患者舌下含服标准化粉尘螨滴剂免疫治疗(sublingual immunotherapy,SLIT)尘螨引起的变应性鼻炎(allergic rhinitis,AR)的疗效。方法回顾性分析了199例接受舌下含服粉尘螨滴剂治疗(sublingual immunotherapy,SLIT)的变应性鼻炎患者的病例资料。其中有163例,年龄4~65岁,平均年龄(19.4±14.6)岁的患者接受了完整3年的治疗。将上述163例患者按照年龄段分为4~13岁年龄组(低龄组,107例,平均年龄为9.1±2.5)和14~65岁年龄组(高龄组,56例,平均年龄为30.7±14.1)。采用组间以及组内前后自身对照的方法,分别在治疗前和治疗后1年、3年评估患者的鼻部症状总评分(total nasal symptom score,TNSS)、药物评分(total medication score,TMS)、视觉模拟量表(visual analogue scale,VAS)评分以及体征评分的变化。采用SPSS 26.0软件对数据进行统计学分析。结果两组患者在经过3年的治疗后TNSS、TMS、VAS评分以及体征评分均无显著性差异。与基线相比,两组患者的TNSS、TMS、VAS评分以及体征评分在治疗1年和3年后均显著降低,差异具有统计学意义(低龄组:治疗1年时Z值分别为-4.750、-3.000、-5.188、-4.125,治疗3年时Z值分别为-6.688、-3.562、-6.812、-4.312;高龄组:治疗1年时Z值分别为-4.990、-4.267、-5.187、-3.414;治疗3年时Z值分别为-6.040、-4.793、-6.237、-4.464,P值均<0.05)。163例患者中有6例发生了轻微不良反应,都出现在免疫治疗的第一个月,且未经治疗自行好转。结论无论对于低龄组还是高龄组,在经过1年或3年舌下含服标准化粉尘螨滴剂治疗尘螨引起的AR均安全有效、耐受性良好。

粉尘螨滴剂联合维生素D滴剂辅助治疗儿童哮喘伴过敏性鼻炎临床观察

目的探讨粉尘螨滴剂联合维生素D滴剂辅助治疗儿童哮喘伴过敏性鼻炎的临床疗效。方法选取江苏省徐州市儿童医院2019年1月至2021年1月收治的哮喘伴过敏性鼻炎患儿112例,按随机数字表法分为对照组和观察组,各56例。两组患儿均予氯雷他定颗粒口服,观察组患儿加用粉尘螨滴剂联合维生素D滴剂(胶囊型)治疗。两组均持续治疗6个月。结果观察组患儿近期总有效率为73.21%,与对照组的67.86%相当(P>0.05);观察组患儿远期总有效率为91.07%,显著高于对照组的71.43%(P<0.05)。两组患儿治疗3个月、6个月喘息、鼻塞、喷嚏、鼻痒评分均显著降低,肺功能指标(用力肺活量,第1秒用力呼气容积,呼气峰流速,用力呼出25%、50%、75%肺活量的呼气流速,以及最大呼气中段流量占预计值百分比)均显著升高,且观察组患儿治疗6个月的喷嚏、鼻痒评分及肺功能指标均改善更显著(P<0.05)。观察组患儿治疗6个月的血清白细胞介素4、白细胞介素5、白细胞介素12及肿瘤坏死因子-α水平均显著低于对照组(P<0.05)。两组患儿均无严重不良反应,血、尿等常规均未检出异常;仅对照组中2例患儿出现喉部不适,3 d后自行缓解。结论粉尘螨滴剂联合维生素D滴剂辅助治疗儿童哮喘伴过敏性鼻炎,可缓解患儿的临床症状,并改善其肺功能。

玉屏风颗粒联合粉尘螨滴剂舌下脱敏治疗小儿变应性鼻炎肺脾气虚证临床研究

目的:观察玉屏风颗粒联合粉尘螨滴剂舌下脱敏治疗小儿变应性鼻炎肺脾气虚证的临床疗效。方法:选择90例变应性鼻炎肺脾气虚证患儿为研究对象,按随机数字表法分为观察组和对照组各45例。对照组给予粉尘螨滴剂舌下脱敏治疗,观察组在对照组基础上给予玉屏风颗粒冲服。1周为1个疗程,连续治疗6个疗程。比较2组临床疗效、鼻部症状评分、体液免疫功能指标及炎性因子水平。结果:观察组总有效率为97.78%,高于对照组86.67%(P<0.05)。治疗后,2组鼻塞、鼻痒、喷嚏、流涕评分均较治疗前降低(P<0.05),且观察组各项评分均低于对照组(P<0.05)。治疗后,2组血清免疫球蛋白M (IgM)、免疫球蛋白G (IgG)水平高于治疗前,免疫球蛋白E (IgE)水平低于治疗前(P<0.05);且观察组IgM、IgG水平高于对照组,IgE水平低于对照组(P<0.05)。治疗后,2组血清白细胞介素-4 (IL-4)、白细胞介素-13 (IL-13)、血管细胞黏附因子-1 (VCAM-1)水平均较治疗前降低(P<0.05),且观察组血清IL-4、IL-13、VCAM-1水平均低于对照组(P<0.05)。结论:玉屏风颗粒联合粉尘螨滴剂舌下脱敏治疗小儿变应性鼻炎肺脾气虚证的疗效显著,有利于改善患儿临床症状,提高机体免疫力,减轻炎症反应。

重组粉尘螨变应原rDer f 27制备及其活性鉴定

目的:制备粉尘螨变应原第27组分(Der f 27)重组蛋白,鉴定其免疫活性。方法:构建pET-28a(+)-Der f 27质粒,转化至E.coli BL21(DE3)感受态细胞,经诱导表达和纯化后,获得重组变应原rDer f 27。IgE-ELISA和IgE-Western blot检测rDer f 27与粉尘螨变应性鼻炎患者血清IgE结合率,分别将变应性鼻炎患者PBMC、人支气管上皮细胞BESA-2B与rDer f 27共孵育24 h后测定细胞因子表达;生物信息学软件分析Der f 27的理化性质和结构。结果:成功构建pET-28a(+)-Der f 27质粒并转化至BL21(DE3)细胞,经IPTG诱导表达和纯化后,SDS-PAGE和Western blot在约48 kD处见特异性条带;IgE-ELISA和IgEWestern blot检测rDer f 27与粉尘螨变应性鼻炎患者血清IgE结合率分别为39.5%和45.5%;与对照组相比,变应性鼻炎患者PBMC与rDer f 27共孵育后,IL-6和IL-8表达升高(P<0.05);BESA-2B细胞与rDer f 27共孵育后,IL-10和TGF-β表达降低,IL-17A和IL-23A表达升高(P<0.05);生物信息学结果显示Der f 27属于丝氨酸蛋白酶抑制物家族,具有此家族通用结构及功能,二级结构主要为α螺旋(42.62%)和无规则卷曲(35.60%)。结论:成功制备了具有较好免疫反应性和免疫原性的重组变应原rDer f 27,并揭示其为变应性鼻炎的重要变应原之一。

变应性鼻炎患者粉尘螨滴剂舌下免疫治疗的依从性及脱落原因分析

目的 探讨变应性鼻炎(AR)患者接受粉尘螨滴剂舌下免疫治疗(SLIT)的依从性及脱落因素。方法 回顾性分析粉尘满滴剂舌下免疫治疗的变应性鼻炎患者652例的资料,记录患者脱落时间及脱落原因。结果 652例患者中,成功随访516例,中途脱落291例,整体依从率为43.60%。随访患者中,<14岁患者依从性较≥14岁患者依从性好,性别因素对脱落率的影响无显著差异,脱落最多的月份为用药的第5、6、7个月。结论 AR患者行SLIT治疗的整体依从性较差,<14岁儿童依从性较≥14岁人群好,加强患者教育,提高随访频率,构建有效的沟通平台有助于提高患者依从性。