Two new rotenoids 4＇,5＇-dihydroxy-6a,12a-dehydrodeguelin （1）,11.4t,5＇-tdhydroxy-6a,12a-dehydrodeguelin （2）, along with two known rotenoids rotenone and deguelin were isolated from the root of Derris elliptica c...Two new rotenoids 4＇,5＇-dihydroxy-6a,12a-dehydrodeguelin （1）,11.4t,5＇-tdhydroxy-6a,12a-dehydrodeguelin （2）, along with two known rotenoids rotenone and deguelin were isolated from the root of Derris elliptica collected in Guangdong Province, China. Their structures were established by extensive spectroscopic analysis.展开更多
OBJECTIVE The emerging role of chronic inflammation is the major degenerative diseases of modern society such as periodontitis,atherosclerosis,rheumatoid arthritis,Parkinson′s disease and even cancer.Eight components...OBJECTIVE The emerging role of chronic inflammation is the major degenerative diseases of modern society such as periodontitis,atherosclerosis,rheumatoid arthritis,Parkinson′s disease and even cancer.Eight components were isolated from Derris laxiflora Benth.,In this study,we found these compounds from Derris laxiflora Benth suppress lipopolysaccharide-induced inflammatory response in murine macrophage(RAW 264.7)cells.METHODS RAW 264.7cells were cultured in DMEM media supplemented with 10%(V/V)heated-inactivated FBS,penicillin 100U·mL-1 and streptomycin 100μg·mL-1.The cells were incubated at 37℃in a humidified atmosphere of 5%CO2in air.RAW264.7cells were seeded in a 24-well plate at a density of 2×105 mL-1 and then incubated with or without LPS(100ng·mL-1)in the absence or presence of compounds for 24 h.Effects of these isolates on NO production were measured indirectly by analysis of nitrite levels using the Griess reaction.Quercetin was used as a positive control.RESULTS ight components were isolated from Derris laxiflora Benth.,including three new pterocarpans 7,6′-dihydroxy-3′-methoxypterocarpan(1),derrispisatin(2),derriscoumaronochromone(3)and three new flavonoids cis-3,4′-dihydroxy-5,7-dimethoxyflavan(4),derriflavanone B(5),iso-lupinenol(6)as well as two known ones,lonchocarpol A(7)and lonchocarpol D(8).The structures of these new compounds were determined by analysis of their spectroscopic data.Raw264.7 cells were treated with the compounds from Derris laxiflora Benth for 24 h.Among them,compounds 5,7 and 8 significantly suppressed the NO production in LPS-treated RAW264.7 cells with IC50 values<10μg·mL-1.CONCLUSION In this study,we found that compounds from Derris laxiflora Benth suppresses lipopolysaccharide-induced inflammatory response in murine Raw264.7 cells.展开更多
Four hitherto unknown prenylated isoflavonoids,named derrisisoflavones H-K(1-4)and one new isoflavan,namely 6-hydroxyisosativan(5),were isolated from the ethanol extract of Derris robusta.Their structures were elucida...Four hitherto unknown prenylated isoflavonoids,named derrisisoflavones H-K(1-4)and one new isoflavan,namely 6-hydroxyisosativan(5),were isolated from the ethanol extract of Derris robusta.Their structures were elucidated on the basis of extensive spectroscopic studies.To our knowledge,derrisisoflavones J(3)and K(4)are the first examples of hydroxyethylated isoflavonoid.展开更多
Four hitherto unknown 6a,11b-dihydroxypterocarpans,namely pterocarpadiols A–D(1–4),were isolated from the ethanol extract of the twigs and leaves of Derris robusta.Their structures were elucidated on the basis of ex...Four hitherto unknown 6a,11b-dihydroxypterocarpans,namely pterocarpadiols A–D(1–4),were isolated from the ethanol extract of the twigs and leaves of Derris robusta.Their structures were elucidated on the basis of extensive spectroscopic analysis.Pterocarpadiols A–D are a kind of very rare 6a,11b-dihydroxypterocarpans,and their presence as markers may be helpful in chemotaxonomical classification.展开更多
基金Research Foundation for University Ph.D.Program,Ministry of Education of P.R.China(No.20070316003).
文摘Two new rotenoids 4＇,5＇-dihydroxy-6a,12a-dehydrodeguelin （1）,11.4t,5＇-tdhydroxy-6a,12a-dehydrodeguelin （2）, along with two known rotenoids rotenone and deguelin were isolated from the root of Derris elliptica collected in Guangdong Province, China. Their structures were established by extensive spectroscopic analysis.
基金The project supported by Department of Industrial Technology,Ministry of Economic Affairs,Chinese TaipeiMedical and Pharmaceutical Industry Technology and Development Center
文摘OBJECTIVE The emerging role of chronic inflammation is the major degenerative diseases of modern society such as periodontitis,atherosclerosis,rheumatoid arthritis,Parkinson′s disease and even cancer.Eight components were isolated from Derris laxiflora Benth.,In this study,we found these compounds from Derris laxiflora Benth suppress lipopolysaccharide-induced inflammatory response in murine macrophage(RAW 264.7)cells.METHODS RAW 264.7cells were cultured in DMEM media supplemented with 10%(V/V)heated-inactivated FBS,penicillin 100U·mL-1 and streptomycin 100μg·mL-1.The cells were incubated at 37℃in a humidified atmosphere of 5%CO2in air.RAW264.7cells were seeded in a 24-well plate at a density of 2×105 mL-1 and then incubated with or without LPS(100ng·mL-1)in the absence or presence of compounds for 24 h.Effects of these isolates on NO production were measured indirectly by analysis of nitrite levels using the Griess reaction.Quercetin was used as a positive control.RESULTS ight components were isolated from Derris laxiflora Benth.,including three new pterocarpans 7,6′-dihydroxy-3′-methoxypterocarpan(1),derrispisatin(2),derriscoumaronochromone(3)and three new flavonoids cis-3,4′-dihydroxy-5,7-dimethoxyflavan(4),derriflavanone B(5),iso-lupinenol(6)as well as two known ones,lonchocarpol A(7)and lonchocarpol D(8).The structures of these new compounds were determined by analysis of their spectroscopic data.Raw264.7 cells were treated with the compounds from Derris laxiflora Benth for 24 h.Among them,compounds 5,7 and 8 significantly suppressed the NO production in LPS-treated RAW264.7 cells with IC50 values<10μg·mL-1.CONCLUSION In this study,we found that compounds from Derris laxiflora Benth suppresses lipopolysaccharide-induced inflammatory response in murine Raw264.7 cells.
基金This work was financially supported by the “Large-scale Compound Library”project of National Development and Reform Commission of China.
文摘Four hitherto unknown prenylated isoflavonoids,named derrisisoflavones H-K(1-4)and one new isoflavan,namely 6-hydroxyisosativan(5),were isolated from the ethanol extract of Derris robusta.Their structures were elucidated on the basis of extensive spectroscopic studies.To our knowledge,derrisisoflavones J(3)and K(4)are the first examples of hydroxyethylated isoflavonoid.
基金This work was financially supported by the“Large-scale Compound Library”project of National Development and Reform Commission of China.
文摘Four hitherto unknown 6a,11b-dihydroxypterocarpans,namely pterocarpadiols A–D(1–4),were isolated from the ethanol extract of the twigs and leaves of Derris robusta.Their structures were elucidated on the basis of extensive spectroscopic analysis.Pterocarpadiols A–D are a kind of very rare 6a,11b-dihydroxypterocarpans,and their presence as markers may be helpful in chemotaxonomical classification.
