A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Target Detection Algorithm in Foggy Scenes Based on Dual Subnets

Under the influence of air humidity,dust,aerosols,etc.,in real scenes,haze presents an uneven state.In this way,the image quality and contrast will decrease.In this case,It is difficult to detect the target in the image by the universal detection network.Thus,a dual subnet based on multi-task collaborative training(DSMCT)is proposed in this paper.Firstly,in the training phase,the Gated Context Aggregation Network(GCANet)is used as the supervisory network of YOLOX to promote the extraction of clean information in foggy scenes.In the test phase,only the YOLOX branch needs to be activated to ensure the detection speed of the model.Secondly,the deformable convolution module is used to improve GCANet to enhance the model’s ability to capture details of non-homogeneous fog.Finally,the Coordinate Attention mechanism is introduced into the Vision Transformer and the backbone network of YOLOX is redesigned.In this way,the feature extraction ability of the network for deep-level information can be enhanced.The experimental results on artificial fog data set FOG_VOC and real fog data set RTTS show that the map value of DSMCT reached 86.56%and 62.39%,respectively,which was 2.27%and 4.41%higher than the current most advanced detection model.The DSMCT network has high practicality and effectiveness for target detection in real foggy scenes.

Artificial Immune Detection for Network Intrusion Data Based on Quantitative Matching Method

Artificial immune detection can be used to detect network intrusions in an adaptive approach and proper matching methods can improve the accuracy of immune detection methods.This paper proposes an artificial immune detection model for network intrusion data based on a quantitative matching method.The proposed model defines the detection process by using network data and decimal values to express features and artificial immune mechanisms are simulated to define immune elements.Then,to improve the accuracy of similarity calculation,a quantitative matching method is proposed.The model uses mathematical methods to train and evolve immune elements,increasing the diversity of immune recognition and allowing for the successful detection of unknown intrusions.The proposed model’s objective is to accurately identify known intrusions and expand the identification of unknown intrusions through signature detection and immune detection,overcoming the disadvantages of traditional methods.The experiment results show that the proposed model can detect intrusions effectively.It has a detection rate of more than 99.6%on average and a false alarm rate of 0.0264%.It outperforms existing immune intrusion detection methods in terms of comprehensive detection performance.

A self-organization formation configuration based assignment probability and collision detection

The formation control of multiple unmanned aerial vehicles(multi-UAVs)has always been a research hotspot.Based on the straight line trajectory,a multi-UAVs target point assignment algorithm based on the assignment probability is proposed to achieve the shortest overall formation path of multi-UAVs with low complexity and reduce the energy consumption.In order to avoid the collision between UAVs in the formation process,the concept of safety ball is introduced,and the collision detection based on continuous motion of two time slots and the lane occupation detection after motion is proposed to avoid collision between UAVs.Based on the idea of game theory,a method of UAV motion form setting based on the maximization of interests is proposed,including the maximization of self-interest and the maximization of formation interest is proposed,so that multi-UAVs can complete the formation task quickly and reasonably with the linear trajectory assigned in advance.Finally,through simulation verification,the multi-UAVs target assignment algorithm based on the assignment probability proposed in this paper can effectively reduce the total path length,and the UAV motion selection method based on the maximization interests can effectively complete the task formation.

Strengthening Network Security: Deep Learning Models for Intrusion Detectionwith Optimized Feature Subset and Effective Imbalance Handling

In recent years,frequent network attacks have highlighted the importance of efficient detection methods for ensuring cyberspace security.This paper presents a novel intrusion detection system consisting of a data prepro-cessing stage and a deep learning model for accurately identifying network attacks.We have proposed four deep neural network models,which are constructed using architectures such as Convolutional Neural Networks(CNN),Bi-directional Long Short-Term Memory(BiLSTM),Bidirectional Gate Recurrent Unit(BiGRU),and Attention mechanism.These models have been evaluated for their detection performance on the NSL-KDD dataset.To enhance the compatibility between the data and the models,we apply various preprocessing techniques and employ the particle swarm optimization algorithm to perform feature selection on the NSL-KDD dataset,resulting in an optimized feature subset.Moreover,we address class imbalance in the dataset using focal loss.Finally,we employ the BO-TPE algorithm to optimize the hyperparameters of the four models,maximizing their detection performance.The test results demonstrate that the proposed model is capable of extracting the spatiotemporal features of network traffic data effectively.In binary and multiclass experiments,it achieved accuracy rates of 0.999158 and 0.999091,respectively,surpassing other state-of-the-art methods.

Microstrip Patch Antenna with an Inverted T-Type Notch in the Partial Ground for Breast Cancer Detections

This study designs a microstrip patch antenna with an inverted T-type notch in the partial ground to detect tumorcells inside the human breast.The size of the current antenna is small enough(18mm×21mm×1.6mm)todistribute around the breast phantom.The operating frequency has been observed from6–14GHzwith a minimumreturn loss of−61.18 dB and themaximumgain of current proposed antenna is 5.8 dBiwhich is flexiblewith respectto the size of antenna.After the distribution of eight antennas around the breast phantom,the return loss curveswere observed in the presence and absence of tumor cells inside the breast phantom,and these observations showa sharp difference between the presence and absence of tumor cells.The simulated results show that this proposedantenna is suitable for early detection of cancerous cells inside the breast.

A Review of Deep Learning-Based Vulnerability Detection Tools for Ethernet Smart Contracts

In recent years,the number of smart contracts deployed on blockchain has exploded.However,the issue of vulnerability has caused incalculable losses.Due to the irreversible and immutability of smart contracts,vulnerability detection has become particularly important.With the popular use of neural network model,there has been a growing utilization of deep learning-based methods and tools for the identification of vulnerabilities within smart contracts.This paper commences by providing a succinct overview of prevalent categories of vulnerabilities found in smart contracts.Subsequently,it categorizes and presents an overview of contemporary deep learning-based tools developed for smart contract detection.These tools are categorized based on their open-source status,the data format and the type of feature extraction they employ.Then we conduct a comprehensive comparative analysis of these tools,selecting representative tools for experimental validation and comparing them with traditional tools in terms of detection coverage and accuracy.Finally,Based on the insights gained from the experimental results and the current state of research in the field of smart contract vulnerability detection tools,we suppose to provide a reference standard for developers of contract vulnerability detection tools.Meanwhile,forward-looking research directions are also proposed for deep learning-based smart contract vulnerability detection.

Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laboratory diagnosis of viral encephalitis is a worldwide challenge.Recently,high-throughput sequencing technology has provided new tools for diagnosing central nervous system infections.Thus,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon sequencing.Methods We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant literature.The detection ability of the primers was verified by software simulation and the detection of known positive samples.Amplicon sequencing was used to validate the samples,and consistency was compared with Sanger sequencing.Results The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted amplicons.The sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger sequencing.Conclusion Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral encephalitis.It is also a useful tool for the high-volume screening of clinical samples.

YOLO-DD:Improved YOLOv5 for Defect Detection

As computer technology continues to advance,factories have increasingly higher demands for detecting defects.However,detecting defects in a plant environment remains a challenging task due to the presence of complex backgrounds and defects of varying shapes and sizes.To address this issue,this paper proposes YOLO-DD,a defect detectionmodel based on YOLOv5 that is effective and robust.To improve the feature extraction process and better capture global information,the vanilla YOLOv5 is augmented with a new module called Relative-Distance-Aware Transformer(RDAT).Additionally,an Information Gap Filling Strategy(IGFS)is proposed to improve the fusion of features at different scales.The classic lightweight attention mechanism Squeeze-and-Excitation(SE)module is also incorporated into the neck section to enhance feature expression and improve the model’s performance.Experimental results on the NEU-DET dataset demonstrate that YOLO-DDachieves competitive results compared to state-of-the-art methods,with a 2.0% increase in accuracy compared to the original YOLOv5,achieving 82.41% accuracy and38.25FPS(framesper second).Themodel is also testedon a self-constructed fabric defect dataset,and the results show that YOLO-DD is more stable and has higher accuracy than the original YOLOv5,demonstrating its stability and generalization ability.The high efficiency of YOLO-DD enables it to meet the requirements of industrial high accuracy and real-time detection.

Real-Time Detection and Instance Segmentation of Strawberry in Unstructured Environment

The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-ronment is a challenging task.Current instance segmentation algorithms for strawberries suffer from issues such as poor real-time performance and low accuracy.To this end,the present study proposes an Efficient YOLACT(E-YOLACT)algorithm for strawberry detection and segmentation based on the YOLACT framework.The key enhancements of the E-YOLACT encompass the development of a lightweight attention mechanism,pyramid squeeze shuffle attention(PSSA),for efficient feature extraction.Additionally,an attention-guided context-feature pyramid network(AC-FPN)is employed instead of FPN to optimize the architecture’s performance.Furthermore,a feature-enhanced model(FEM)is introduced to enhance the prediction head’s capabilities,while efficient fast non-maximum suppression(EF-NMS)is devised to improve non-maximum suppression.The experimental results demonstrate that the E-YOLACT achieves a Box-mAP and Mask-mAP of 77.9 and 76.6,respectively,on the custom dataset.Moreover,it exhibits an impressive category accuracy of 93.5%.Notably,the E-YOLACT also demonstrates a remarkable real-time detection capability with a speed of 34.8 FPS.The method proposed in this article presents an efficient approach for the vision system of a strawberry-picking robot.

Learning Discriminatory Information for Object Detection on Urine Sediment Image

In clinical practice,the microscopic examination of urine sediment is considered an important in vitro examination with many broad applications.Measuring the amount of each type of urine sediment allows for screening,diagnosis and evaluation of kidney and urinary tract disease,providing insight into the specific type and severity.However,manual urine sediment examination is labor-intensive,time-consuming,and subjective.Traditional machine learning based object detection methods require hand-crafted features for localization and classification,which have poor generalization capabilities and are difficult to quickly and accurately detect the number of urine sediments.Deep learning based object detection methods have the potential to address the challenges mentioned above,but these methods require access to large urine sediment image datasets.Unfortunately,only a limited number of publicly available urine sediment datasets are currently available.To alleviate the lack of urine sediment datasets in medical image analysis,we propose a new dataset named UriSed2K,which contains 2465 high-quality images annotated with expert guidance.Two main challenges are associated with our dataset:a large number of small objects and the occlusion between these small objects.Our manuscript focuses on applying deep learning object detection methods to the urine sediment dataset and addressing the challenges presented by this dataset.Specifically,our goal is to improve the accuracy and efficiency of the detection algorithm and,in doing so,provide medical professionals with an automatic detector that saves time and effort.We propose an improved lightweight one-stage object detection algorithm called Discriminatory-YOLO.The proposed algorithm comprises a local context attention module and a global background suppression module,which aid the detector in distinguishing urine sediment features in the image.The local context attention module captures context information beyond the object region,while the global background suppression module emphasizes objects in uninformative backgrounds.We comprehensively evaluate our method on the UriSed2K dataset,which includes seven categories of urine sediments,such as erythrocytes(red blood cells),leukocytes(white blood cells),epithelial cells,crystals,mycetes,broken erythrocytes,and broken leukocytes,achieving the best average precision(AP)of 95.3%while taking only 10 ms per image.The source code and dataset are available at https://github.com/binghuiwu98/discriminatoryyolov5.

Detection accuracy of target accelerations based on vortex electromagnetic wave in keyhole space

The influence of the longitudinal acceleration and the angular acceleration of detecting target based on vortex electromagnetic waves in keyhole space are analyzed.The spectrum spreads of different orbital angular momentum(OAM)modes in different non-line-of-sight situations are simulated.The errors of target accelerations in detection are calculated and compared based on the OAM spectra spreading by using two combinations of composite OAM modes in the keyhole space.According to the research,the effects about spectrum spreads of higher OAM modes are more obvious.The error in detection is mainly affected by OAM spectrum spreading,which can be reduced by reasonably using different combinations of OAM modes in different practical situations.The above results provide a reference idea for investigating keyhole effect when vortex electromagnetic wave is used to detect accelerations.

Local saliency consistency-based label inference for weakly supervised salient object detection using scribble annotations

Recently,weak supervision has received growing attention in the field of salient object detection due to the convenience of labelling.However,there is a large performance gap between weakly supervised and fully supervised salient object detectors because the scribble annotation can only provide very limited foreground/background information.Therefore,an intuitive idea is to infer annotations that cover more complete object and background regions for training.To this end,a label inference strategy is proposed based on the assumption that pixels with similar colours and close positions should have consistent labels.Specifically,k-means clustering algorithm was first performed on both colours and coordinates of original annotations,and then assigned the same labels to points having similar colours with colour cluster centres and near coordinate cluster centres.Next,the same annotations for pixels with similar colours within each kernel neighbourhood was set further.Extensive experiments on six benchmarks demonstrate that our method can significantly improve the performance and achieve the state-of-the-art results.

Enhancing Dense Small Object Detection in UAV Images Based on Hybrid Transformer

Transformer-based models have facilitated significant advances in object detection.However,their extensive computational consumption and suboptimal detection of dense small objects curtail their applicability in unmanned aerial vehicle(UAV)imagery.Addressing these limitations,we propose a hybrid transformer-based detector,H-DETR,and enhance it for dense small objects,leading to an accurate and efficient model.Firstly,we introduce a hybrid transformer encoder,which integrates a convolutional neural network-based cross-scale fusion module with the original encoder to handle multi-scale feature sequences more efficiently.Furthermore,we propose two novel strategies to enhance detection performance without incurring additional inference computation.Query filter is designed to cope with the dense clustering inherent in drone-captured images by counteracting similar queries with a training-aware non-maximum suppression.Adversarial denoising learning is a novel enhancement method inspired by adversarial learning,which improves the detection of numerous small targets by counteracting the effects of artificial spatial and semantic noise.Extensive experiments on the VisDrone and UAVDT datasets substantiate the effectiveness of our approach,achieving a significant improvement in accuracy with a reduction in computational complexity.Our method achieves 31.9%and 21.1%AP on the VisDrone and UAVDT datasets,respectively,and has a faster inference speed,making it a competitive model in UAV image object detection.

Ionically Imprinting-Based Copper(Ⅱ)Label-Free Detection for Preventing Hearing Loss

Copper is a microelement with important physiological functions in the body.However,the excess copper ion(Cu^(2+))may cause severe health problems,such as hair cell apoptosis and the resultant hearing loss.Therefore,the assay of Cu^(2+)is important.We integrate ionic imprinting technology(IIT)and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads(IIHBs)as a low-cost and high-specificity platform for Cu^(2+)detection.The IIHBs have a macroporous microstructure,uniform size,vivid structural color,and magnetic responsiveness.When incubated in solution,IIHBs recognize Cu^(2+)and exhibit a reflective peak change,thereby achieving label-free detection.In addition,benefiting from the IIT,the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100μmol·L^(-1).These features indicated that the developed IIHBs are promising candidates for Cu^(2+)detection,particularly for the prevention of hearing loss.

Improving Federated Learning through Abnormal Client Detection and Incentive

Data sharing and privacy protection are made possible by federated learning,which allows for continuous model parameter sharing between several clients and a central server.Multiple reliable and high-quality clients must participate in practical applications for the federated learning global model to be accurate,but because the clients are independent,the central server cannot fully control their behavior.The central server has no way of knowing the correctness of the model parameters provided by each client in this round,so clients may purposefully or unwittingly submit anomalous data,leading to abnormal behavior,such as becoming malicious attackers or defective clients.To reduce their negative consequences,it is crucial to quickly detect these abnormalities and incentivize them.In this paper,we propose a Federated Learning framework for Detecting and Incentivizing Abnormal Clients(FL-DIAC)to accomplish efficient and security federated learning.We build a detector that introduces an auto-encoder for anomaly detection and use it to perform anomaly identification and prevent the involvement of abnormal clients,in particular for the anomaly client detection problem.Among them,before the model parameters are input to the detector,we propose a Fourier transform-based anomaly data detectionmethod for dimensionality reduction in order to reduce the computational complexity.Additionally,we create a credit scorebased incentive structure to encourage clients to participate in training in order tomake clients actively participate.Three training models(CNN,MLP,and ResNet-18)and three datasets(MNIST,Fashion MNIST,and CIFAR-10)have been used in experiments.According to theoretical analysis and experimental findings,the FL-DIAC is superior to other federated learning schemes of the same type in terms of effectiveness.

An Efficient Modelling of Oversampling with Optimal Deep Learning Enabled Anomaly Detection in Streaming Data

Recently,anomaly detection(AD)in streaming data gained significant attention among research communities due to its applicability in finance,business,healthcare,education,etc.The recent developments of deep learning(DL)models find helpful in the detection and classification of anomalies.This article designs an oversampling with an optimal deep learning-based streaming data classification(OS-ODLSDC)model.The aim of the OSODLSDC model is to recognize and classify the presence of anomalies in the streaming data.The proposed OS-ODLSDC model initially undergoes preprocessing step.Since streaming data is unbalanced,support vector machine(SVM)-Synthetic Minority Over-sampling Technique(SVM-SMOTE)is applied for oversampling process.Besides,the OS-ODLSDC model employs bidirectional long short-term memory(Bi LSTM)for AD and classification.Finally,the root means square propagation(RMSProp)optimizer is applied for optimal hyperparameter tuning of the Bi LSTM model.For ensuring the promising performance of the OS-ODLSDC model,a wide-ranging experimental analysis is performed using three benchmark datasets such as CICIDS 2018,KDD-Cup 1999,and NSL-KDD datasets.

A Deep Learning Approach for Landmines Detection Based on Airborne Magnetometry Imaging and Edge Computing

Landmines continue to pose an ongoing threat in various regions around the world,with countless buried landmines affecting numerous human lives.The detonation of these landmines results in thousands of casualties reported worldwide annually.Therefore,there is a pressing need to employ diverse landmine detection techniques for their removal.One effective approach for landmine detection is UAV(Unmanned Aerial Vehicle)based AirborneMagnetometry,which identifies magnetic anomalies in the local terrestrial magnetic field.It can generate a contour plot or heat map that visually represents the magnetic field strength.Despite the effectiveness of this approach,landmine removal remains a challenging and resource-intensive task,fraughtwith risks.Edge computing,on the other hand,can play a crucial role in critical drone monitoring applications like landmine detection.By processing data locally on a nearby edge server,edge computing can reduce communication latency and bandwidth requirements,allowing real-time analysis of magnetic field data.It enables faster decision-making and more efficient landmine detection,potentially saving lives and minimizing the risks involved in the process.Furthermore,edge computing can provide enhanced security and privacy by keeping sensitive data close to the source,reducing the chances of data exposure during transmission.This paper introduces the MAGnetometry Imaging based Classification System(MAGICS),a fully automated UAV-based system designed for landmine and buried object detection and localization.We have developed an efficient deep learning-based strategy for automatic image classification using magnetometry dataset traces.By simulating the proposal in various network scenarios,we have successfully detected landmine signatures present in themagnetometry images.The trained models exhibit significant performance improvements,achieving a maximum mean average precision value of 97.8%.

Highly Differentiated Target Detection under Extremely Low-Light Conditions Based on Improved YOLOX Model

This paper expounds upon a novel target detection methodology distinguished by its elevated discriminatory efficacy,specifically tailored for environments characterized by markedly low luminance levels.Conventional methodologies struggle with the challenges posed by luminosity fluctuations,especially in settings characterized by diminished radiance,further exacerbated by the utilization of suboptimal imaging instrumentation.The envisioned approach mandates a departure from the conventional YOLOX model,which exhibits inadequacies in mitigating these challenges.To enhance the efficacy of this approach in low-light conditions,the dehazing algorithm undergoes refinement,effecting a discerning regulation of the transmission rate at the pixel level,reducing it to values below 0.5,thereby resulting in an augmentation of image contrast.Subsequently,the coiflet wavelet transform is employed to discern and isolate high-discriminatory attributes by dismantling low-frequency image attributes and extracting high-frequency attributes across divergent axes.The utilization of CycleGAN serves to elevate the features of low-light imagery across an array of stylistic variances.Advanced computational methodologies are then employed to amalgamate and conflate intricate attributes originating from images characterized by distinct stylistic orientations,thereby augmenting the model’s erudition potential.Empirical validation conducted on the PASCAL VOC and MS COCO 2017 datasets substantiates pronounced advancements.The refined low-light enhancement algorithm yields a discernible 5.9%augmentation in the target detection evaluation index when compared to the original imagery.Mean Average Precision(mAP)undergoes enhancements of 9.45%and 0.052%in low-light visual renditions relative to conventional YOLOX outcomes.The envisaged approach presents a myriad of advantages over prevailing benchmark methodologies in the realm of target detection within environments marked by an acute scarcity of luminosity.

A New Vehicle Detection Framework Based on Feature-Guided in the Road Scene

Vehicle detection plays a crucial role in the field of autonomous driving technology.However,directly applying deep learning-based object detection algorithms to complex road scene images often leads to subpar performance and slow inference speeds in vehicle detection.Achieving a balance between accuracy and detection speed is crucial for real-time object detection in real-world road scenes.This paper proposes a high-precision and fast vehicle detector called the feature-guided bidirectional pyramid network(FBPN).Firstly,to tackle challenges like vehicle occlusion and significant background interference,the efficient feature filtering module(EFFM)is introduced into the deep network,which amplifies the disparities between the features of the vehicle and the background.Secondly,the proposed global attention localization module(GALM)in the model neck effectively perceives the detailed position information of the target,improving both the accuracy and inference speed of themodel.Finally,the detection accuracy of small-scale vehicles is further enhanced through the utilization of a four-layer feature pyramid structure.Experimental results show that FBPN achieves an average precision of 60.8% and 97.8% on the BDD100K and KITTI datasets,respectively,with inference speeds reaching 344.83 frames/s and 357.14 frames/s.FBPN demonstrates its effectiveness and superiority by striking a balance between detection accuracy and inference speed,outperforming several state-of-the-art methods.