Objective: To study the relationship between the expression of muscarinic receptor subtype M3R and the decomposition of phosphatidylinositol (PI). Methods: The [3H]-IP contents were detected with [3H] incorporation in...Objective: To study the relationship between the expression of muscarinic receptor subtype M3R and the decomposition of phosphatidylinositol (PI). Methods: The [3H]-IP contents were detected with [3H] incorporation in human cultured detrusor muscle cells after being stimulated by carbachol, atropine, methoctramine, and 4-DAMP, respectively. Results: The [3H]-IP contents were increased with the elevation of carbachol concentration. Both atropine and 4-DAMP significantly inhibited the effect of carbachol on PI decomposition (P<0.01), but methoctramine did not. No significant difference was seen in the effect of atropine and 4-DAMP on PI decomposition. Conclusion: M3R is closely related to the decomposition of PI, the second messenger molecule IP3.展开更多
文摘Objective: To study the relationship between the expression of muscarinic receptor subtype M3R and the decomposition of phosphatidylinositol (PI). Methods: The [3H]-IP contents were detected with [3H] incorporation in human cultured detrusor muscle cells after being stimulated by carbachol, atropine, methoctramine, and 4-DAMP, respectively. Results: The [3H]-IP contents were increased with the elevation of carbachol concentration. Both atropine and 4-DAMP significantly inhibited the effect of carbachol on PI decomposition (P<0.01), but methoctramine did not. No significant difference was seen in the effect of atropine and 4-DAMP on PI decomposition. Conclusion: M3R is closely related to the decomposition of PI, the second messenger molecule IP3.
