Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

The environment,especially the minimum temperature,affects summer maize grain yield by regulating ear differentiation and grain development

Ear differentiation,grain development and their interaction with factors in the growing environment,such as temperature,solar radiation and precipitation,greatly influence grain number and grain weight,and ultimately affect summer maize production.In this study,field experiments involving different sowing dates were conducted over three years to evaluate the effects of temperature factors,average solar radiation and total precipitation on the growth process,ear differentiation,fertilization characteristics,grain filling and yield of summer maize varieties with different growth durations.Four hybrids were evaluated in Huang-Huai-Hai Plain(HHHP),China from 2018 to 2020 with five different sowing dates.The results showed that the grain yield formation of summer maize was strongly impacted by the environment from the silking(R1)to milking(R3)stage.Average minimum temperature(AT_(min))was the key environmental factor that determined yield.Reductions in the length of the growing season(r=–0.556,P<0.01)and the total floret number on ear(R^(2)=0.200,P<0.001)were found when AT_(min) was elevated from the emerging(VE)to R1 stage.Both grain-filling rate(R^(2)=0.520,P<0.001)and the floret abortion rate on ear(R^(2)=0.437,P<0.001)showed quadratic relationships with AT_(min) from the R1 to physiological maturity(R6)stage,while the number of days after the R1 stage(r=–0.756,P<0.01)was negatively correlated with AT_(min).An increase in AT_(min) was beneficial for the promotion of yield when it did not exceeded a certain level(above 23°C during the R1–R3 stage and 20–21°C during the R1-R6 stage).Enhanced solar radiation and precipitation during R1–R6 increased the grain-filling rate(R^(2)=0.562,P<0.001 and R^(2)=0.229,P<0.05,respectively).Compared with short-season hybrids,full-season hybrids showed much greater suitability for a critical environment.The coordinated regulation of AT_(min),ear differentiation and grain development at the pre-and post-silking stages improved maize yield by increasing total floret number and grain-filling rate,and by reducing the floret abortion rate on ear.

Environment Development and Health Safety System in Rural Areas of Bangladesh (Noakhali and Lakshmipur Districts)

At present time, Bangladesh is developing its position in all sectors of world prosperity. Economic development of Bangladesh depends on working ability of whole population of this country, although Bangladesh is not developed in health status of its whole population. A large percentage of people live in rural areas without any proper shelter. This study revealed the environmental development and health safety system in rural areas of Bangladesh, which is located in Noakhali and Lakshmipur districts of Bangladesh. Many sections were studied, which are the main structure of environmental development and health safety system of southeastern region of Bangladesh. The study found that there were three types of handicraft enterprises in the surveyed area such as handicraft input, like palm leaf, factories, Betel nut businesses, and Coconut businesses were headed and owned by the women and they were unaware of their health, environment and waste management or reduction of pollution. This study also reveals that microentrepreneurs did not ensure purified water supply and hygienic toilets within enterprises in the study area. The study also found that a significant portion of the microentrepreneurs are workers, and their family members were always fewer users of protective equipment during the COVID-19 pandemic. This study identified that most MEs faced transport or carrying facilities or big costing problems, including problems in available services for raw materials in the high category. In contrast, the high cost of transport facilities was very intensive in the study area. Moreover, it found that Microentrepreneurs (MEs) faced high product marketing costs for transport facilities, and a lack of proper pricing of the products in the study area. In addition, it explored that most of the microentrepreneurs did not receive sufficient credit support, and they faced negative attitudes and delayed responses from the credit services providers in the study area. It also found that the majority of the microentrepreneurs argued that capital support, loan, aid, technological assistance, infrastructural assistance, availability of skilled labor forces, availability of raw materials, and getting the government assistance and services were the major reasons for the highest growth of the enterprises in the study area. In addition, entire MEs had no knowledge and understanding of climate change in the study area. It is worth mentioning that MEs were not aware of climate change and its impacts on nature in the handicrafts sector and in their daily lives. A significant portion of the MEs took safety measures against risk and protected themselves and workers within the enterprises in the study area. MEs had various demands such as infrastructural development, economic assistance (/aid/loan/grants/financial), training and development, assistance for environment development, and machinery assistance for promoting their business. MEs argued that the implementation of their suggestion is the key to improve the current situation of the enterprises. The MEs suggested appointing expert workers, ensuring quality inputs, training workers and reserving products carefully.

The IDD Transcription Factors:Their Functions in Plant Development and Environmental Response

INDETERMINATE-DOMAIN proteins(IDDs)are a plant-specific transcription factor family characterized by a conserved ID domain with four zinc finger motifs.Previous studies have demonstrated that IDDs coordinate a diversity of physiological processes and functions in plant growth and development,including floral transition,plant architecture,seed and root development,and hormone signaling.In this review,we especially summarized the latest knowledge on the functions and working models of IDD members in Arabidopsis,rice,and maize,particularly focusing on their role in the regulatory network of biotic and abiotic environmental responses,such as gravity,temperature,water,and pathogens.Understanding these mechanisms underlying the function of IDD proteins in these processes is important for improving crop yields by manipulating their activity.Overall,the review offers valuable insights into the functions and mechanisms of IDD proteins in plants,providing a foundation for further research and potential applications in agriculture.

Impact of coupling coordination development of port and city environments on urban economic competitiveness:Evidence from the coastal port cities in eastern China

Promoting the coupling coordination development of port and its hinterland city environments is an important way to improve urban economic competitiveness.Based on relevant data of 13 coastal port cities in eastern China from 2000 to 2018,this study explores the coupling coordination development of port and city environments and its impact on urban economic competitiveness by constructing the coupling coordination degree model and the panel threshold model.The research results show that:(1)In terms of the coupling coordination development of port and city environments,most coastal ports and their hinterland cities are in a state of moderate or serious disorder.Overall,the degree of coupling coordination of port and city environments needs to be further improved;(2)The coupling coordination degree of port and city environments has a significant impact on urban economic competitiveness,and this effect gradually increases with the development of the ports and the urban economy.Among the variables that impact the urban economic competitiveness,fixed assets investment and foreign trade are significant factors that can enhance urban economic competitiveness.(3)At present,there is a“U-shaped”relationship between the coupling coordination degree of port-city environments and the urban economic competitiveness.This relationship lies on the right side of the inflection point of the“U-shaped”curve.Therefore,following the concept of assigning priority to ecological development,expanding fixed assets investment and actively developing foreign trade can further enhance the urban economic competitiveness.

New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

The Sustainable Development Pathway of the Biomedical Industry Based on Environmental,Social,and Governance(ESG)Concepts

There is a growing global awareness of environmental,social,and governance(ESG)concerns.The biopharmaceutical industry is an important field that affects human health and well-being,and its sustainable development is now the industry’s focus.Based on the current state of the green development of China’s biopharmaceutical industry,the article proposes suggestions and paths for promoting the industry to better fulfill its social responsibilities and protect the environment while pursuing economic benefits.By doing so,the industry can make a greater contribution to global public health and become an important factor in promoting human health and social prosperity.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Relation between Economic Development Level and Resource and Environment Carrying Capacity of Central Area of Yunnan Province Based on Decoupling Analysis

Based on the decoupiing theory and method, an indicator system was built for the relation between economic development level and resource and environment carrying capacity. And the study was carried out on decoupling degree and temporal changes of economic development level and resource and environment carrying ca- pacity in the central area of Yunnan Province. Results indicated that （i） the economic development level and resource and environment carrying capacity in the central area of Yunnan Province mainly experienced strong decoupling, weak decoupling, and ex- pansive negative coupling, and in general it was strong decoupling, and it took on re- verse ＂N＂ in temporal changes. （ii） Change rate of economic development level in the central area of Yunnan Province was greater than zero, but the amplitude of change was not large, while the change rate of resource and environment carrying capacity was negative in 2007-2008, and it was positive in the rest years; from 2007, it took on gradual expansion trend, and scissors difference gradually increased after experi- encing reverse ＂V＂ change. （iii） The strong decoupling was the main situation and it reached the peak value in T5 period and T6 pedod.

Eco-environment Evolution and Its Sustainable Development Strategies in Western China

Natural condition in Western China is relatively poor and regional economy level is low. The ecological environment has been seriously damaged by population growth and over-exploitation of natural resources. It is very important for coordinating the regional development and safeguarding ecological security to discuss the eco-environment evolution trend and its sustainable development strategies in Western China. Based on analyzing documents and relative research,the changes of main ecological and environmental problems in the western region,such as degradation of forest and grassland ecosystems,shrinkage of wetland,desertification,water and soil erosion,etc. were synthetically discussed. Then,according to the development trend,some countermeasures for eco-environment protection and rehabilitation and sustainable development were proposed.

Research on the Low-carbon-high-value Agriculture Technology in the Comprehensive Improvement of Intra-county Environment Pollution——Taking the Development of Cultivation and Breeding Industry in the Ecological Cyclic Agricultural Garden in Shaoshan Irri

The comprehensive improvement strategy of intra-county environment pollution in the city and countryside was searched.By the research method which combined the microscopic view,the macroscopic view with the dynamic perspective,the seriousness of rural water quality,soil and atmospheric pollution in Xiangxiang,Xiangtan and the surrounding areas in Shaoshan irrigated area was revealed.The control measure which was 'four-dimensional pollution in the city and countryside'—— low-carbon-high-value agriculture and the technology innovation was proposed.The low-carbon-high-value technology innovation industrialization demonstration in three parts which included the pre-production,mid-production and post-production deep-processing of cultivation and breeding industry in the ecological cyclic agricultural garden in Shaoshan irrigated area was the driving force.We tried to propel the low-carbon ecological cultivation and breeding industry which included the paddy rice,grass,tree,medicinal herbs and pig,cow,chick,duck,fish.We wanted to relieve the structural unbalance of previous cultivation and breeding industry,'cheap grain hurting the farmers' and the short-leg problem of social-economic-ecological benefit.The results showed that the low-carbon-high-value agricultural system was a poly-generation technology system which promoted the multi-level and grading utilization,saved the energy,reduced the consumption and cleaned the production based on the ecology.

Research on Environmental Problems during Island Tourism Development in China

Due to the weakness of island eco-environment and the dependence of tourism development on island environment,it urgently needs to make a study on environmental problems during island tourism development,so as to provide a reference for the sustainable development of island tourism.The paper has concluded Chinese island environmental characteristics,analyzed the current situation of island tourism development and discussed environmental problems during island tourism development.On this basis,it has proposed some suggestions for island tourism environment protection.It should take island eco-environment protection as the premise and complete relevant laws and regulations;take foreign island tourism development as the reference,plan scientifically and build island eco-tourism mode;improve island infrastructures,prevent environmental pollution and protect island tourism environment with the government as the guidance;establish island environment monitoring station and tourism information monitoring station;enhance eco-environment protection education and cultivate responsible tourists and tourism staff;and intensify island supervision and regularize island tourism order.

Study on Environmental Capacity and Sustainable Development Strategy of Scenic Areas ——Taking the Scenic Area of Slender West Lake in Yangzhou for an Example

Taking the scenic area of Slender West Lake in Yangzhou for an example, the quantitative evaluation model of environmental capacity and impact factors limiting sustainable development of scenic area were put forward, and targeted control measures were correspondingly proposed.

Development of Sedimentary Environment in the Northeastern South China Sea Since the Last Glacial Stage

Core D （21°23′02″N, 116°47′13″E, water depth 405 m） was sampled from the upper slope from the northeastern South China Sea （SCS） and applied to analyze the sedimentary environmental change in this sea area since the last glacial stage. The results of grain size analysis, diatom analysis and detrital mineral analysis were well matched. We divided the core D into two layers. The surface sand layer （0 - 2 cm） consisted of residual sediments, which might be originally the sediment in the late Pleistocene and later suffered from being transformed in the post glacial transgression. The lower layer （2 - 130 cm） was quite different from the surface one, which might mainly result from a neritic sedimentary environment in the last glacial stage. Two sedimentary cycles could be detected in the core D： regression during Marine isotope stage （MIS） 4 to transgression during MIS 3 and regression during MIS 2 to transgression during the post glacial.

Study of Coordinated Development of Urbanization and the Environment

With the rapid promotion of urbanization, urban environmental pollution has become increasingly serious. The urban environmental pollution is closely related to unilateral pursuit of economic growth, insufficient investment in urban environmental control, lack of environmental protection mechanism, etc . To promote urbanization, it is necessary to set up the development concept ecological cities and towns, establish scientific assessment mechanism for local governments, increase the investment in environmental control in villages and towns, optimize urban distribution, and achieve the coordinated development of urbanization and the environment.

Current Situation and Future Development of“Affordance”in College English Learning Environment in Information Age

With the increasing development of economy and society,the 21st century will surely become an era of rapid development of information technology.Based on the macro and micro levels of education in China,this paper introduces the"affordance theory"to analyze and discuss the current situation of College English learning environment in China,and puts forward new goals and principles to promote the future development of College English learning environment in order to better promote its effective transformation.

Quantitative Evaluation of Sustainable Development and Eco-Environmental Carrying Capacity in Water-Deficient Regions:A Case Study in the Haihe River Basin,China

Quantitative assessment of development sustainability could be a challenge to regional management and planning, especially for areas facing great risks of water shortage. Surface-water decline and groundwater over-pumping have caused serious environmental problems and limited economic development in many regions all around the world. In this paper, a framework for quantitatively evaluating development sustainability was established with water-related eco-environmental carrying capacity （EECC） as the core measure. As a case study, the developed approach was applied to data of the Haihe River Basin, China, during 1998 through 2007. The overall sustainable development degree （SDD） is determined to be 0.39, suggesting that this rate of development is not sustainable. Results of scenario analysis revealed that overshoot, or resource over- exploitation, of the Basin＇s EECC is about 20% for both population and economy. Based on conditions in the study area in 2007, in order to achieve sustainable development, i.e., SDD〉0.70 in this study, the EECC could support a population of 108 million and gross domestic product （GDP） of 2.72 trillion CNY. The newly developed approach in quantifying ecoenvironmental carrying capacity is anticipated to facilitate sustainable development oriented resource management in waterdeficient areas.

A STUDY ON THE FORECAST AND REGULATION OF COORDINATED DEVELOPMENT OF URBAN ENVIRONMENT AND ECONOMY IN GUANGZHOU

This article introduces the concepts of coordinated development of economy and environment and formulas of coordinated degree. Results obtained are that the coordinated degree in 1992 is over 0.87.The variation tendencies on coordinated development of urb

Issues of Agricultural Sustainable Development in Environment with Limited Resources——A Case Study of Xinjiang

Based on natural resources in Xinjiang, some problems existing in agricul- tural sustainable development in Xinjiang were explored, such as simple agricultural structure （unbalanced development in different regions）, dispersed production pattern, and lower efficient water use for agriculture. Furthermore, some countermeasures for Xinjiang agricultural sustainable development were proposed as well, including ad- justment of agricultural structure for optimization of resource allocation, strict control of population for improvement of people education, strengthening of S＆T innovation for popularization of sustainable development, and construction of macro-adjustment mechanism of resources use for virtuous environment.