Objective:To observe the effect of Icariin on diabetic myocardial hypertrophy and explore its molecular mechanism.Methods:C57BL/6 mice were randomly divided into Ctrl group(normal control group),DM group(STZ intraperi...Objective:To observe the effect of Icariin on diabetic myocardial hypertrophy and explore its molecular mechanism.Methods:C57BL/6 mice were randomly divided into Ctrl group(normal control group),DM group(STZ intraperitoneal injection model),and DM+ICA group(diabetic C57BL/6 mice by intragastric Icariin solution 80mg/kg/d,for 3 consecutive weeks).Real-time quantitative PCR was used to detect myocardial hypertrophy markers BNP andβ-MHC.Western blotting was used to detect myocardial AMPK,p-AMPK,mTOR,p-mTOR,LC3B and Beclin1 protein expression.Echocardiogram was used to detect left ventricular mass and ejection fraction.Results:Compared with the normal control group,the expression of myocardial hypertrophy markers BNP andβ-MHC mRNA in diabetic mice were significantly increased;the expression of phosphorylated AMPK protein,autophagy-related protein LC3B and Beclin1 were significantly increased,and the expression of phosphorylated mTOR protein is significantly reduced;the left ventricular mass is significantly increased.The above changes can be reversed after treatment with Icariin,but the effect of Icariin is blocked by the autophagy inhibitor rapamycin.Conclusion:Icariin may inhibit autophagy and reduce diabetic myocardial hypertrophy through AMPK-mTOR signaling pathway.展开更多
基金Project of Medical and Health Research,Gansu Province(No.GSWSKY2020-13)。
文摘Objective:To observe the effect of Icariin on diabetic myocardial hypertrophy and explore its molecular mechanism.Methods:C57BL/6 mice were randomly divided into Ctrl group(normal control group),DM group(STZ intraperitoneal injection model),and DM+ICA group(diabetic C57BL/6 mice by intragastric Icariin solution 80mg/kg/d,for 3 consecutive weeks).Real-time quantitative PCR was used to detect myocardial hypertrophy markers BNP andβ-MHC.Western blotting was used to detect myocardial AMPK,p-AMPK,mTOR,p-mTOR,LC3B and Beclin1 protein expression.Echocardiogram was used to detect left ventricular mass and ejection fraction.Results:Compared with the normal control group,the expression of myocardial hypertrophy markers BNP andβ-MHC mRNA in diabetic mice were significantly increased;the expression of phosphorylated AMPK protein,autophagy-related protein LC3B and Beclin1 were significantly increased,and the expression of phosphorylated mTOR protein is significantly reduced;the left ventricular mass is significantly increased.The above changes can be reversed after treatment with Icariin,but the effect of Icariin is blocked by the autophagy inhibitor rapamycin.Conclusion:Icariin may inhibit autophagy and reduce diabetic myocardial hypertrophy through AMPK-mTOR signaling pathway.
