To enhance the anesthetic efficacy and reduce toxic side effects,a strategy is proposed involving the utilization of general anesthetics of Propofol(Pro)and Eto-midate(Eto)to synergistic inhibition GABA receptors simul...To enhance the anesthetic efficacy and reduce toxic side effects,a strategy is proposed involving the utilization of general anesthetics of Propofol(Pro)and Eto-midate(Eto)to synergistic inhibition GABA receptors simultaneously.Four-in-one molecular aggregates were prepared to implement this strategy,which comprised of Pro and Eto with the bridging molecule monoglyceride monooleate(GMO)and sur-factant F127 through intermolecular forces.The blood-brain barrier(BBB)targeted lactoferrin(LF)is affixed to their surface,obtaining thefinal molecular aggregates.By employing lactoferrin enrich aggregates to the BBB,followed by ultrasound combine microbubbles to open the BBB,a remarkable 4.5-fold enhancement in brain drug delivery was achieved.The molecular aggregates group maintained sta-ble parameters of heart rate,diastolic blood pressure,and systolic blood pressure.A notable increase of more than twice therapeutic index(TI)value was observed,implying their higher anesthesia efficiency and reduced toxicity.Electroencephalo-gram(EEG)experiments demonstrate a significant elevation in the proportion of θ waves from 28%to 80%for aggregates,accompanied by a nearlyfivefold reduc-tion in the proportion ofθwaves,meaning a significant improvement in synergistic anesthesia effectiveness(interaction index 0.289)with lower drug dosage.Further-more,mouse immunofluorescence brain slice experiments suggest Pro and Eto enter the GABA receptor simultaneously,resulting in synergistic inhibition of GABA receptors.展开更多
目的探讨磁共振成像(MRI)技术在乳腺良恶性病变确定方面的应用。方法选取2017年2月至2018年12月我院收治的乳腺疾病患者150例,所有患者均接受手术治疗,且在手术治疗前接受MRI检查。在手术过程中取所有患者的病灶样本组织进行病理诊断,...目的探讨磁共振成像(MRI)技术在乳腺良恶性病变确定方面的应用。方法选取2017年2月至2018年12月我院收治的乳腺疾病患者150例,所有患者均接受手术治疗,且在手术治疗前接受MRI检查。在手术过程中取所有患者的病灶样本组织进行病理诊断,并将所有的MRI检查结果与病理诊断结果相比。观察MRI在乳腺疾病患者良性及恶性评估方面的诊断效能。结果本文共128例乳腺病变患者当中,恶性疾病患者96例,共存在112个恶性疾病病灶;良性疾病患者32例,共存在38个良性疾病病灶。所有患者的平均病灶大小为(4.7±1.2)cm。3.0 T MRI增强扫描共检出111个恶性疾病病灶,39个良性疾病病灶,其跟病理诊断结果相比,准确性为:88.7%(133/150),灵敏性为:90.2%(101/112),特异性为:84.2%(32/38)。结论给予乳腺病变患者进行MRI检查,能够有效提高乳腺病变患者在良性及恶性方面的鉴别诊断效果。展开更多
Irregular plagues of house mice,Mus musculus,incur major economic impacts on agricultural production in Australia.The efficacy of zinc phosphide(ZnP),the only registered broadacre control agent for mice,is reported as ...Irregular plagues of house mice,Mus musculus,incur major economic impacts on agricultural production in Australia.The efficacy of zinc phosphide(ZnP),the only registered broadacre control agent for mice,is reported as increasingly variable.Have mice become less sensitive over time or are they taking a sub-lethal dose and develop-ing aversion?In this laboratory study,the sensitivity of mice(wild caught;outbred laboratory strain)was assessed using oral gavage of a range of ZnP concentrations.The estimated LD50 values(72–79 mg ZnP/kg body weight)were similar for each mouse group but are significantly higher than previously reported.The willingness of mice to consume ZnP-coated grains was determined.ZnP-coated grains(50 g ZnP/kg grain)presented in the absence of alternative food were consumed and 94%of wild mice died.Mice provided with alternative food and ZnP-coated wheat grains(either 25 or 50 g ZnP/kg grain)consumed toxic and non-toxic grains,and mortality was lower(33–55%).If a sublethal amount of ZnP-coated grain was consumed,aversion occurred,mostly when alternative food was present.The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed.Under laboratory conditions,ZnP-coated grains coated with a new higher dose(50 g ZnP/kg grain)were readily consumed.Consumption of toxic grain occurred when alternative food was available but was decreased.Our unambiguousfindings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.展开更多
基金Young Top-notch Talents of“High-level Talents Special Support Program”of Shaanxi ProvinceNational Natural Science Foundation of China,Grant/Award Numbers:81971290,82102074。
文摘To enhance the anesthetic efficacy and reduce toxic side effects,a strategy is proposed involving the utilization of general anesthetics of Propofol(Pro)and Eto-midate(Eto)to synergistic inhibition GABA receptors simultaneously.Four-in-one molecular aggregates were prepared to implement this strategy,which comprised of Pro and Eto with the bridging molecule monoglyceride monooleate(GMO)and sur-factant F127 through intermolecular forces.The blood-brain barrier(BBB)targeted lactoferrin(LF)is affixed to their surface,obtaining thefinal molecular aggregates.By employing lactoferrin enrich aggregates to the BBB,followed by ultrasound combine microbubbles to open the BBB,a remarkable 4.5-fold enhancement in brain drug delivery was achieved.The molecular aggregates group maintained sta-ble parameters of heart rate,diastolic blood pressure,and systolic blood pressure.A notable increase of more than twice therapeutic index(TI)value was observed,implying their higher anesthesia efficiency and reduced toxicity.Electroencephalo-gram(EEG)experiments demonstrate a significant elevation in the proportion of θ waves from 28%to 80%for aggregates,accompanied by a nearlyfivefold reduc-tion in the proportion ofθwaves,meaning a significant improvement in synergistic anesthesia effectiveness(interaction index 0.289)with lower drug dosage.Further-more,mouse immunofluorescence brain slice experiments suggest Pro and Eto enter the GABA receptor simultaneously,resulting in synergistic inhibition of GABA receptors.
文摘目的探讨磁共振成像(MRI)技术在乳腺良恶性病变确定方面的应用。方法选取2017年2月至2018年12月我院收治的乳腺疾病患者150例,所有患者均接受手术治疗,且在手术治疗前接受MRI检查。在手术过程中取所有患者的病灶样本组织进行病理诊断,并将所有的MRI检查结果与病理诊断结果相比。观察MRI在乳腺疾病患者良性及恶性评估方面的诊断效能。结果本文共128例乳腺病变患者当中,恶性疾病患者96例,共存在112个恶性疾病病灶;良性疾病患者32例,共存在38个良性疾病病灶。所有患者的平均病灶大小为(4.7±1.2)cm。3.0 T MRI增强扫描共检出111个恶性疾病病灶,39个良性疾病病灶,其跟病理诊断结果相比,准确性为:88.7%(133/150),灵敏性为:90.2%(101/112),特异性为:84.2%(32/38)。结论给予乳腺病变患者进行MRI检查,能够有效提高乳腺病变患者在良性及恶性方面的鉴别诊断效果。
基金supported by the Grains Research and Development Corporation(GRDC)through project CSP1804-012RTX,and CSIRO Health and Biosecurityapproved by the CSIRO Wildlife and Large Animal Ethics Committee(Approval No 2019-23)conform to the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes.
文摘Irregular plagues of house mice,Mus musculus,incur major economic impacts on agricultural production in Australia.The efficacy of zinc phosphide(ZnP),the only registered broadacre control agent for mice,is reported as increasingly variable.Have mice become less sensitive over time or are they taking a sub-lethal dose and develop-ing aversion?In this laboratory study,the sensitivity of mice(wild caught;outbred laboratory strain)was assessed using oral gavage of a range of ZnP concentrations.The estimated LD50 values(72–79 mg ZnP/kg body weight)were similar for each mouse group but are significantly higher than previously reported.The willingness of mice to consume ZnP-coated grains was determined.ZnP-coated grains(50 g ZnP/kg grain)presented in the absence of alternative food were consumed and 94%of wild mice died.Mice provided with alternative food and ZnP-coated wheat grains(either 25 or 50 g ZnP/kg grain)consumed toxic and non-toxic grains,and mortality was lower(33–55%).If a sublethal amount of ZnP-coated grain was consumed,aversion occurred,mostly when alternative food was present.The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed.Under laboratory conditions,ZnP-coated grains coated with a new higher dose(50 g ZnP/kg grain)were readily consumed.Consumption of toxic grain occurred when alternative food was available but was decreased.Our unambiguousfindings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.
